• 전자공학회논문지C
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• 제35C권10호
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• pp.30-37
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• 1998

1.2㎛ 더블 폴리 부유게이트 트랜지스터로 구성된 아날로그 메모리가 CMOS 표준공정에서 제작되었다. 효율적인 프로그래밍을 위해 일반적인 아날로그 메모리에서 사용되었던 불필요한 초기 소거 동작을 제거하였으며 프로그래밍과 읽기의 경로를 동일하게 가져감으로서 읽기 동작 시에 발생하는 증폭기의 DC offset 문제를 근본적으로 제거하였다. 어레이의 구성에서 특정 셀을 주변의 다른 셀들로부터 격리시키는 패스 트랜지스터 대신에 Vmid라는 별도의 전압을 사용하였다. 실험 결과 아날로그 메모리가 디지털 메모리의 6비트에 해당하는 정밀도를 보였으며 프로그래밍 시에 선택되지 않은 주변의 셀들에 간섭 효과가 없는 것으로 확인되었다. 마지막으로, 아날로그 어레이를 구성하는 셀은 특이한 모양의 인젝터 구조를 가지고 있으며, 이것은 아날로그 메모리가 특별한 공정 없이도 트랜지스터의 breakdown 전압 아래에서 프로그래밍 되도록 하였다.

• Journal of Ginseng Research
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• 제44권1호
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• pp.79-85
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• 2020

Background: Helicobacter pylori increases reactive oxygen species (ROS) and induces oxidative DNA damage and apoptosis in gastric epithelial cells. DNA damage activates DNA damage response (DDR) which includes ataxia-telangiectasia-mutated (ATM) activation. ATM increases alternative reading frame (ARF) but decreases mouse double minute 2 (Mdm2). Because p53 interacts with Mdm2, H. pylori-induced loss of Mdm2 stabilizes p53 and induces apoptosis. Previous study showed that Korean Red Ginseng extract (KRG) reduces ROS and prevents cell death in H. pylori-infected gastric epithelial cells. Methods: We determined whether KRG inhibits apoptosis by suppressing DDRs and apoptotic indices in H. pylori-infected gastric epithelial AGS cells. The infected cells were treated with or without KRG or an ATM kinase inhibitor KU-55933. ROS levels, apoptotic indices (cell death, DNA fragmentation, Bax/Bcl-2 ratio, caspase-3 activity) and DDRs (activation and levels of ATM, checkpoint kinase 2, Mdm2, ARF, and p53) were determined. Results: H. pylori induced apoptosis by increasing apoptotic indices and ROS levels. H. pylori activated DDRs (increased p-ATM, p-checkpoint kinase 2, ARF, p-p53, and p53, but decreased Mdm2) in gastric epithelial cells. KRG reduced ROS and inhibited increase in apoptotic indices and DDRs in H. pylori-infected gastric epithelial cells. KU-55933 suppressed DDRs and apoptosis in H. pylori-infected gastric epithelial cells, similar to KRG. Conclusion: KRG suppressed ATM-mediated DDRs and apoptosis by reducing ROS in H. pylori-infected gastric epithelial cells. Supplementation with KRG may prevent the oxidative stress-mediated gastric impairment associated with H. pylori infection.

• Molecules and Cells
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• 제23권3호
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• pp.304-315
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• 2007

Fusarium graminearum causes a serious scab disease of small grains in Korea. The nucleotide sequence of the genomic RNA of a double-stranded RNA (dsRNA) virus, Fusarium graminearum virus-DK21 (FgV-DK21), from F. graminearum strain DK21, which is associated with hypovirulence in F. graminearum, was determined and compared to the genome sequences of other mycoviruses, including Cryponectria hypoviruses. The FgV-DK21 dsRNA consists of 6,624 nucleotides, excluding the 3'-terminal poly(A) tail. The viral genome has 53- and 46-nucleotide 5' and 3' untranslated regions (UTRs), respectively, and five putative open reading frames. A phylogenetic analysis of the deduced amino acid sequence of ORF1, which encodes a putative RNA-dependent RNA polymerase, and those of other mycoviruses revealed that this organism forms a distinct virus clade with other hypoviruses, and is more distantly related to other mycoviruses (3.8 to 24.0% identity). However, pairwise sequence comparisons of the nucleotide and deduced amino acid sequences of ORFs 2 through 5 revealed no close relationships to other protein sequences currently available in GenBank. Analyses of RNA accumulation by Northern blot and primer extension indicated that these putative gene products are expressed from at least two different subgenomic RNAs (sgRNAs), in contrast to the cases in other hypoviruses. This study suggests the existence of a new, as yet unassigned, genus of mycoviruses that exhibits a potex-like genome organization and sgRNA accumulation.

• 영어영문학
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• 제55권6호
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• pp.1013-1034
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• 2009

The space of the Other assumes the space of Barthes's multiplicity and Foucault's transdiscursive position, and, therefore, aims at becoming the locus in which the speaking subject and the hearing subjects are supposed to communicate and constitute as if they were situated in the pscychoanalytic session. However, the wall of untranslatibility across language and cultures still exist there in the space of the Other in the form of trauma and aggressivity, as Lacan demonstrate perceptively through the reading of Kant avec Sade. In short, Lacan regards the moral commandment (to love one's neighbor as oneself) as the obstacle in the Freud's myth of transgression, and interprets this in terms of the emergence of the Other. Freud understands that the aggressivity in the subject's own heart was inherent in all humans, and that one's neighbor would be evil. Lacan goes beyond Freud and articulates that the aggressivity in the imaginary relation with the Other in the mirror stage insures that an evil inheres in the very being of humanity. A global phenomenon of the diasporic identities and hybridity, the phenomenon which has been represented by the complicated intermixture of terms which span from diaspora, postcolonialism, postnationalism. and transnationalism can be clarified, if they are put in the context of the ethics of Othering or becoming the Other. The ethics of Othering presupposes the situation in which the diasporic subjects encounter the lack of the cross-cultural negotiation and communication. The purpose of this paper is to demonstrate how the poetics of Other and the logic of the ethics of Othering can explain the postmodern or transmodern world which has become deterritorialized, diasporic, and transnational as well as how one can encounter the results of diasporic and postcolonial double consciousness, a consciousness which is a discursive category for multicultural or cross-cultural, focusing on the concept of liminality/interstitiality

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 1997년도 Progress and Future Development of Sericultural Science and Technology 40th Anniversary Commemoration Symposium
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• pp.73-101
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• 1997

Baculoviruses are characterized by large double-stranded circular DNA genomes and rod-shaped enveloped virions. Bombyx mori nucleopolyhedrovirus(BmNPV) is a major pathogen, which causes severe damage in sericulture. Currently, BmNPV is recogtnized as an improtant tool in molecular biology, especially for expression of useful genes in B.mori cells and silkworm larvae. Our laboratories have focused on the studies of the molecular mechanisms of BmNPV replication and the application of BmNPV to agriculture and medicine. The entire nucleotide sequence of the BmNPV genome has recently determined. The BmNPV genome possessed 135 putative genes and 7 homologous repeated sequence (hrs) regions. Relatively little space, a few to a few hundred base-pairs, was observed between the open reading frames and hrs. Termination codons often overlapped. These results showed a compactly packde BmNPV genome. Based on comparative sequence analyses, we speculated that the ancestor of BmNPV was a baculovirus similar to Autographa californica NPV(AcNPV). The function of the BmNPV genes were characterized by gene deletion analysis; p35 was found to be involved in blocking apoptosis and cysteine proteinase was found to be involved in horizontal virus transmission by degrading viral-infected larval host. By AcNPV and BmNPV coinfection experiments, we identified a BmNPV gene involved in expanding host specificity of AcNPV. The identified gene was likely encoded a DNA helicase based on the amino acid sequence analysis; a few amino acid substitutions in the putative DNA helicase gene resulted in the expansion of host range of AcNPV. These findings indicate that BmNPV evolved within a short period from an AcNPV-like ancestral virus due to rapid evolution including specific amino acid substitutions and gene deletions/insertions.

• Journal of Microbiology and Biotechnology
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• 제29권5호
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• pp.696-703
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• 2019

Cronobacter sakazakii is an opportunistic pathogen causing serious infections in neonates. In this study, a bacteriophage ${\Phi}CS01$, which infects C. sakazakii, was isolated from swine feces and its morphology, growth parameters, and genomic analysis were investigated. Transmission electron microscopy revealed that ${\Phi}CS01$ has a spherical head and is 65.74 nm in diameter with a 98.75 nm contracted tail, suggesting that it belongs to the family Myoviridae. The major viral proteins are approximately 71 kDa and 64 kDa in size. The latent period of ${\Phi}CS01$ was shown to be 60 min, and the burst size was 90.7 pfu (plaque-forming units)/infected cell. Bacteriophage ${\Phi}CS01$ was stable at $4-60^{\circ}C$ for 1 h and lost infectivity after 1 h of heating at $70^{\circ}C$. Infectivity remained unaffected at pH 4-9 for 2 h, while the bacteriophage was inactivated at pH <3 or >10. The double-stranded ${\Phi}CS01$ DNA genome consists of 48,195 base pairs, with 75 predicted open reading frames. Phylogenetic analysis is closely related to that of the previously reported C. sakazakii phage ESP2949-1. The newly isolated ${\Phi}CS01$ shows infectivity in the host bacterium C. sakazakii, indicating that it may be a promising alternative to antibacterial agents for the removal of C. sakazakii from powdered infant formulas.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.272-279
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• 2021

Two genes encoding probable α-ʟ-arabinofuranosidase (E.C. 3.2.1.55) isozymes (ABFs) with 92.3% amino acid sequence identity, ABF51A and ABF51B, were found from chromosomes 3 and 5 of Saccharomycopsis fibuligera KJJ81, an amylolytic yeast isolated from Korean wheat-based nuruk, respectively. Each open reading frame consists of 1,551 nucleotides and encodes a protein of 517 amino acids with the molecular mass of approximately 59 kDa. These isozymes share approximately 49% amino acid sequence identity with eukaryotic ABFs from filamentous fungi. The corresponding genes were cloned, functionally expressed, and purified from Escherichia coli. SfABF51A and SfABF51B showed the highest activities on p-nitrophenyl arabinofuranoside at 40~45℃ and pH 7.0 in sodium phosphate buffer and at 50℃ and pH 6.0 in sodium acetate buffer, respectively. These exoacting enzymes belonging to the glycoside hydrolase (GH) family 51 could hydrolyze arabinoxylo-oligosaccharides (AXOS) and arabino-oligosaccharides (AOS) to produce only ʟ-arabinose, whereas they could hardly degrade any polymeric substrates including arabinans and arabinoxylans. The detailed product analyses revealed that both SfABF51 isozymes can catalyze the versatile hydrolysis of α-(1,2)- and α-(1,3)-ʟ-arabinofuranosidic linkages of AXOS, and α-(1,2)-, α-(1,3)-, and α-(1,5)-linkages of linear and branched AOS. On the contrary, they have much lower activity against the α-(1,2)- and α-(1,3)-double-substituted substrates than the single-substituted ones. These hydrolases could potentially play important roles in the degradation and utilization of hemicellulosic biomass by S. fibuligera.

• 대중서사연구
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• 제27권1호
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• pp.341-357
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• 2021

본 논문은 프랑스의 언론인이자 철학자인 디디에 에리봉이 미술사학자 에른스트 곰브리치를 대상으로 진행한 인터뷰를 기초로 1991년 처음 출간되었던 『이미지가 우리에게 말해주는 것』의 한국판 새 번역을 앞두고 이 책이 시사하는 학문의 대중적 서사로서의 가능성을 가늠해보는 것을 목표로 한다. 에리봉은 앞서 뒤메질, 레비스트로스와의 기념비적 대담을 출판한 바 있는데, 이들의 지적 사유와 문제의식을 명철한 질문을 통해 대화 형식(dialogue)으로 쉽게 풀어냈다. 곰브리치라는 인물이 학문으로서 미술사가 자리 잡은 과정에서 수행한 독보적인 역할과 고유의 업적뿐만 아니라 그의 인생을 가로지르는 시대적 상황과 지성사의 흐름을 짚어내는 해당 저서는 학문이 이용하는 특수한 방법론, 공식과 용어, 특정한 정신 상태를 대화라는 친근한 서사를 빌려 해설한다. 여기에 주목하여 본 논문은 역사가로서 곰브리치 특유의 언어를 간략히 분석하고 이를 통해 미술사라는 학문이 의존하는 서사적 방법론을 논한 후, 책에서 에리봉이 던지는 질문과 답변을 정리하는 방식을 탐구하여 아카데미아와 그 해설이라는 두 가지 영역의 융합을 학제적 측면에서 살펴본다. 이러한 분석은 에리봉이 곰브리치라는 학자에게서 찾은 학자이면서 대중을 끌어안은 이야기꾼으로서의 이중의 정체성과도 깊은 관련이 있다. 이로써 미술사라는 학문을 텍스트로서 재조명하고, 대화라는 새로운 장르와의 결합 가능성을 타진해보고자 한다.

• 식물병연구
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• 제30권2호
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• pp.157-164
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• 2024

Jack bean (Canavalia ensiformis) is one of healthy products for fermented or functional food in Korea and is widely distributed and cultivated worldwide. During August 2022, Jack bean plants showing symptoms of yellow flecks, chlorosis, necrotic spots and mosaic were observed in Jangheung-gun, South Korea. By transmission electron microscopy, flexuous filamentous virus particles of approximately 750×13 nm in size were observed in the symptomatic leaf samples. The infection of a Korean isolate of clover yellow vein virus (ClYVV-Ce-JH) was confirmed using double antibody sandwich enzyme-linked sorbent assay, reverse transcription polymerase chain reaction and high-throughput sequencing. The complete genome sequence of ClYVV-Ce-JH consists of 9,549 nucleotides (nt) excluding the poly (A) tail and encodes 3,072 amino acids (aa), with an AUG start and UAG stop codon, containing one open reading frame that is typical of a potyvirus polyprotein. The polyprotein of ClYVV-Ce-JH was divided into ten proteins and each protein's cleavage sites were determined. The coat protein (CP) and polyprotein of ClYVV-Ce-JH were compared at the nt and aa levels with those of the previously reported 14 ClYVV isolates. ClYVV-Ce-JH shared 92.62% to 99.63% and 93.39% to 98.05% at the CP and polyprotein homology. To our knowledge, this is the first report of a Korean isolate of ClYVV from Jack bean plants and the complete genome sequence of a ClYVV Jack bean isolate in the world.

• 동양고전연구
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• 제71호
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• pp.231-258
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• 2018

본고는 "천의소감언해"의 이본 간의 차이를 문체론적 측면에서 살펴보고 그 차이의 요인에 대하여 고찰하였다. 목판본과 필사본의 언해는 전제된 독자층에 따라 달라질 수 있고 이본 간의 문체적 차이는 독자층의 확대성에 따라 달라질 수 있다. 이에 본고에서는 언해자인 화자와 독자층인 수신자의 상관관계 속에서 화자의 의도에 따라 문체의 효과가 어떻게 나타나는지를 고찰하였다. II장에서는 이본 간에 나타나는 문체론적 차이를 수용자에 대한 언해자의 경의 표현, 정서 표현, 공적 관계 의식 표현으로 구분하여 살펴보았다. 경의 표현은 목판본에서보다 필사본에서 더 적극적으로 드러난다. 존칭 주어 표지인 '겨 오셔'와 화자 겸양의 표지인 '- -'은 필사본에서 훨씬 빈번하게 나타난다. 긍정적 정서 표현을 위해 필사본에서는 감탄형 어미가 사용되었는데 이는 수용자의 말이나 행동에 대하여 감정적으로 강하게 동조하기 위한 것으로 보인다. 반면 목판본에서는 역신이나 역모에 가담한 죄인을 하대하는 의미로 인명 뒤에 '-이'를 붙여 부르고 있다. 그리고 필사본에서의 긍정문이 목판본에서는 이중부정문으로 표현되었는데, 이는 목판본에서 임금의 왕위 계승에 대한 의의와 당위성을 더욱 강조하고자 한 것이다. 언해자의 공적 관계 의식은 고유어 지명의 훈독 표기 방식을 통해 살펴볼 수 있다. 또한 공적 관계를 드러내는 목판본에서는 고유어의 지명을 한자로 나타내는 것에 비해 필사본에서는 고유어로 표기하고 있다. III장에서는 이본 간의 문체론적 차이가 무엇에서 비롯되었는지에 대하여 살펴보았다. 차이의 요인을 언해의 목적에 따른 수용자의 계층과 범위, 수용자에 대한 언해자의 태도, 언해자와 수용자의 대면적 상황으로 구분하여 고찰하였다.