• BMB Reports
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• 제55권3호
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• pp.125-135
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• 2022

Continuously renewing the proteome, translation is exquisitely controlled by a number of dedicated factors that interact with the ribosome. The RNA helicase DDX3 belonging to the DEAD box family has emerged as one of the critical regulators of translation, the failure of which is frequently observed in a wide range of proliferative, degenerative, and infectious diseases in humans. DDX3 unwinds double-stranded RNA molecules with coupled ATP hydrolysis and thereby remodels complex RNA structures present in various protein-coding and noncoding RNAs. By interacting with specific features on messenger RNAs (mRNAs) and 18S ribosomal RNA (rRNA), DDX3 facilitates translation, while repressing it under certain conditions. We review recent findings underlying these properties of DDX3 in diverse modes of translation, such as cap-dependent and cap-independent translation initiation, usage of upstream open reading frames, and stress-induced ribonucleoprotein granule formation. We further discuss how disease-associated DDX3 variants alter the translation landscape in the cell.

• Clinical Endoscopy
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• 제55권4호
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• pp.473-479
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• 2022

Over the last few years, capsule endoscopy has been established as a fundamental device in the practicing gastroenterologist's toolbox. Its utilization in diagnostic algorithms for suspected small bowel bleeding, Crohn's disease, and small bowel tumors has been approved by several guidelines. The advent of double-balloon enteroscopy has significantly increased the therapeutic possibilities and release of multiple devices (single-balloon enteroscopy and spiral enteroscopy) aimed at improving the performance of small bowel enteroscopy. Recently, some important innovations have appeared in the small bowel endoscopy scene, providing further improvement to its evolution. Artificial intelligence in capsule endoscopy should increase diagnostic accuracy and reading efficiency, and the introduction of motorized spiral enteroscopy into clinical practice could also improve the therapeutic yield. This review focuses on the most recent studies on artificial-intelligence-assisted capsule endoscopy and motorized spiral enteroscopy.

• The Plant Pathology Journal
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• 제27권3호
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• pp.285-290
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• 2011

Fusarium graminearum virus 2 (FgV2) infects Fusarium graminearum strain 98-8-60 and has at least five segments of double-stranded RNAs (dsRNAs), denoted as dsRNA-1 to dsRNA-5. In this study, the genome of FgV2 was sequenced and its phylogenetic relationship with other mycoviruses was analyzed. The lengths of FgV2 dsRNAs 1-5 ranged from 2414 to 3580 base pairs (bp). The 5' and 3' untranslated regions (UTRs) are highly conserved, and each dsRNA segment had 78-105 and 84-306 bp of 5' and 3' UTRs, respectively. Each dsRNA segment contained a single open reading frame (ORF). Computer analysis of dsRNA-1 revealed a putative open reading frame (ORF) that shows high sequence identity with an RNA-dependent RNA polymerase (RdRp) containing eight conserved motifs. dsRNAs 2-5 also each contain one putative ORF coding for products of unknown function. The sequences of FgV2 dsRNA-2 and dsRNA-3 have significant sequence identity with Magnaporthe oryzae chrysovirus 1 (MoCV1) dsRNA-3 and -4, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis of the putative RdRp protein, FgV2 was found to form a distinct virus clade with Aspergillus mycovirus 1816 and MoCV1 in the family Chrysoviridae.

• 한국자기학회지
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• 제16권3호
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• pp.186-191
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• 2006

자기터널접합 기반의 MRAM(magnetic random access memory)은 자기저항효과를 응용하는 메모리소자로서 비휘발성과 고속 정보처리가 가능할 뿐만 아니라 고집적화 할 수 있는 차세대 통합형 비휘발성 메모리이다. 그러나 기존의 메모리 소자들에 비해 스위칭 산포가 크고, 기록마진(writing margin)이 확보되지 않아 아직까지는 고집적화가 어려운 실정이다. 최근 포화자화가 낮은 NiFeSiB 및 CoFeSiB과 같은 비정질 강자성체를 자기터널접합의 자유층 재료로 사용하여 스위칭 자기장의 거대화를 크게 감소시켜 MRAM의 기록마진을 높이는 연구결과에 관해 정리하여 보았다. 그리고 이러한 물질을 이용하여 자기터널접합의 재생마진(reading margin)과 관련된 터널자기저항비의 인가전압의존성을 저감시킬 수 있었다. 본고에서는 나노자기소자 기술의 중요한 분야인 MRAM의 기술발전 방향과 연구사례를 소개하고자 한다.

• 한국산학기술학회논문지
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• 제12권9호
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• pp.4133-4137
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• 2011

신뢰성 있는 다중태그 인식은 최근 다중태그 애플리케이션 이슈 중의 하나이다. 하지만, 데이터 확보 단계에서 다중태그 리더를 통한 신뢰성 있는 다중태그 인식은 리더간의 충돌, 소음, 태그가 부착된 물건들의 이동 등으로 발생하는 거짓양성인식, 거짓음성인식, 비 인식같은 신뢰성 없는 인식으로 인하여 신뢰서 있는 데이터를 확보하는데 어려움을 겪고 있다. 따라서 본 논문은 다중태그 리더를 통한 인식에서 발생되는 이러한 문제점들을 해결하기 위하여 먼저 성능평가 기준을 소개하고, 1) 수신된 신호 강도 표시기 (RSSI)을 이용한 최소 중첩인식공간 설정방식, 2)시-공간 분할 처리방식, 3) 큰 사이즈의 이중 태그 부착 방식등과 같은 3가지 해결방안을 제시하였다. 그리고 본 논문은 멀티 RFID 리더가 설치된 스마트 사무실에서 태그의 성공 인식률 계산을 통하여 제안된 방법의 성능개선을 보여주었다.

• 융합정보논문지
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• 제7권2호
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• pp.59-65
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• 2017

창의적 모듈형 완구의 동작 구현을 위하여 동작을 기록하고 또한 읽어서 반복 동작하는 방식의 움직임이 요구된다. 이 때 완구 동작용 모터 출력축에는 전위차계를 사용하여 절대 회전각을 읽어서 제어를 수행하게 된다. 하지만 전위차계의 감지 영역의 불안정한 부분이 일정 영역에 존재하게 되는데 이로 인한 모터 제어의 불안정을 가져올 수 있다. 본 논문에서는 2개의 전위차계를 한 축에 장착시켜 안정된 영역을 각각 읽어서 1회전 절대각을 찾는 알고리즘을 제안한다. 그리고 다중 회전을 수행 시 필요로 하는 보정 알고리즘에 대해서도 기술하였다. 제안된 방식은 실제 토포보 모듈라 완구에 적용하여 동작을 기록하고 반복 동작을 수행하여 효과적으로 동작됨을 보였다. 아울러 다 회전 동작을 기록하고 동작 시켜 제안된 방식의 유용성을 제시하였다. 향 후 다양한 동작을 통하여 기록과 재생의 기능을 확대해 나갈 것이다.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.563-569
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• 2004

The DNA sequence of the chitosanase gene (choK) from $\beta$-Proteobacterium KNU3 showed an 1,158-bp open reading frame that encodes a protein of 386 amino acids with a novel 74 signal peptide. The degenerated primers based on the partial deduced amino acid sequences from MALDI- TOF MS analyses yielded the 820 bp of the PCR product. Based on this information, double inverse PCR cloning experiments, which use the two specific sets of PCR primers rather than single set primers, identified the unknown 1.2 kb of the choK gene. Subsequently, a 1.8 kb of full choK gene was cloned from another PCR cloning experiment and it was then subcloned into pGEM T-easy and pUC18 vectors. The recombinant E. coli clone harboring recombinant pUC18 vector produced a clear halo around the colony in the glycol chitosan plates. The recombinant ChoK protein was secreted into medium in a mature form while the intracellular ChoK was produced without signal peptide cleavage. The activity staining of PAGE showed that the recombinant ChoK protein was identical to the chitosanase of wild-type. The comparison of deduced amino acid sequences of choK revealed that there is 92% identity with that of Sphingobacterium multivorum chitosanase. Judging from the conserved module in other bacterial chitosanases, chitosanase of KNU3 strain (ChoK) belongs to the family 80 of glycoside hydrolases.

• Parasites, Hosts and Diseases
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• 제45권2호
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• pp.87-94
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• 2007

In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slip-page heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV If-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus.

• 대한토목학회논문집
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• 제29권3A호
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• pp.251-258
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• 2009

이 논문은 정다각형 중실 단면을 갖는 최강보에 관한 연구이다. 이 연구에서 보의 체적은 항상 일정하다. 이러한 보에 집중하중과 만재 사다리꼴 분포하중이 작용하는 경우에 탄성곡선의 미분방정식을 유도하고 이를 중적분법을 이용하여 풀어 정적 거동을 산정하였다. 미분방정식의 정적분은 Simpson 공식을 이용하였다. 수치해석 예에서는 고정-고정 보 및 고정-회전보를 채택하였고, 단면깊이의 형상함수로는 선형, 포물선형 및 정현형의 함수를 채택하였다. 이 연구에서 얻은 수치해석의 결과로부터 보의 정적 최대거동값이 최소가 되는 단면형상 즉 최강단면비를 산정하였다.

• Parasites, Hosts and Diseases
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• 제62권2호
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• pp.226-237
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• 2024

Ticks, blood-sucking ectoparasites, spread diseases to humans and animals. Haemaphysalis longicornis is a significant vector for tick-borne diseases in medical and veterinary contexts. Identifying protective antigens in H. longicornis for an anti-tick vaccine is a key tick control strategy. Enolase, a multifunctional protein, significantly converts D-2-phosphoglycerate and phosphoenolpyruvate in glycolysis and gluconeogenesis in cell cytoplasm. This study cloned a complete open reading frame (ORF) of enolase from the H. longicornis tick and characterized its transcriptional and silencing effect. We amplified the full-length cDNA of the enolase gene using rapid amplification of cDNA ends. The complete cDNA, with an ORF of 1,297 nucleotides, encoded a 432-amino acid polypeptide. Enolase of the Jeju strain H. longicornis exhibited the highest sequence similarity with H. flava (98%), followed by Dermacentor silvarum (82%). The enolase motifs identified included N-terminal and C-terminal regions, magnesium binding sites, and several phosphorylation sites. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that enolase mRNA transcripts were expressed across all developmental stages of ticks and organs such as salivary gland and midgut. RT-PCR showed higher transcript levels in syn-ganglia, suggesting that synganglion nerves influence enolase's role in tick salivary glands. We injected enolase double-stranded RNA into adult unfed female ticks, after which they were subsequently fed with normal unfed males until they spontaneously dropped off. RNA interference significantly (P<0.05) reduced feeding and reproduction, along with abnormalities in eggs (no embryos) and hatching. These findings suggest enolase is a promising target for future tick control strategies.