• 유기물자원화
• /
• 제23권1호
• /
• pp.70-81
• /
• 2015

Helicase는 NTP 결합의 화학적 에너지를 이용하여 이중가닥의 DNA와 RNA를 단일가닥으로 분해하여 다양한 생체반응에 기여하는 단백질로 알려져 있으며, 이 중 DEAD-box의 단백질은 주로 RNA와 관련된 대부분의 생화학적 반응에 작용하는 ATP 의존성 helicase로 알려져 있다. 또한 이 단백질 부류에 속하는 DEAD-box3 (DDX3) gene은 척추동물뿐만 아니라 무척추동물에서의 유성 생식과 무성 생식에서 생식세포 발달 및 재생과정 중 줄기세포 분화에 중요한 역할을 하는 인자로 알려져 있다. 이에 본 연구는 강한 재생능력을 가진 것으로 알려져 있는 팔딱이 지렁이(Perionyx excavatus)에서 DDX3 gene을 동정하고 그 발현양상을 알아보고자 환대를 포함하는 성체 지렁이의 두부를 절단하여 total RNA를 추출하고, 이를 주형으로 RT-PCR을 수행하여 full length의 DDX3 gene인 Pe-DDX3를 검출하였다. Pe-DDX3는 607개 아미노산 서열로 이루어져 있으며, DEAD-box 단백질 그룹 내에서 특이적으로 보존되어 있는 9개의 motif가 존재하고 있다. 다른 분류군에 속하는 동물들과의 multiple alignment를 통해 서열 내에 보존되어 있는 아미노산 서열을 확인할 수 있었으며, 아미노산 차원에서의 계통수 분석을 통해 DDX3 (PL10) 하부그룹에 속하는 것을 알 수 있었으며, 또한, 같은 그룹에 속하는 동물 중 P. dumerilii의 PL10a, b 단백질과 가장 가까운 유연관계를 확인 할 수 있었다.

• 한국의류학회지
• /
• 제41권5호
• /
• pp.840-857
• /
• 2017

In 1995, the costumes were restored in an unknown woman's tomb at the Jeonju Lee's family cemetery in Gapyeong, Gyeonggi-Do, and there are currently nine items remaining. In this study, we first introduced these 9 relics to academia and analyzed the morphological characteristics of the costumes. We also estimated the time and person of burial compared with costumes unearthed from other burials. Jeogories (short jacket) are all four items. One of these items was unusual in shape, and the upper part of the seop (gusset) was wrinkled and shaped. This type of Jeogori appears only till the 1520s in other tombs. There are also two skirts, one of which is characterized by a superimposed pattern. The top and bottom / middle part of the skirt were rolled up, and the skirt, which was double-rolled up in this way, was first discovered. An analysis of the shape of Jeogori and skirt indicated that the person buried in the Jeonju Lee's family tomb was believed to have survived until the early 16th century. Based on these estimated periods, as a result of looking at the genealogy of Jeonju Lee's family, it was concluded that the tomb was probably a woman named Lee Geum Myeong (李金命) born in the mid-15C and died between the late 15C and the early 16C.

• Journal of Ginseng Research
• /
• 제42권2호
• /
• pp.192-198
• /
• 2018

Background: Ginseng has been used as an ergogenic agent, although evidence for its effectiveness is weak. A randomized, double-blind, placebo-controlled clinical trial was conducted to evaluate the effect of a ginsenoside complex (UG0712) on changes in exercise performance. Methods: Sedentary individuals (n = 117) were randomly assigned into one of three groups: low-dose ginsenoside supplementation (100 mg/d, n = 39), high-dose ginsenoside supplementation (500 mg/d, n = 39), or a placebo group (500 mg/d, n = 39). All participants underwent a supervised 12-wk aerobic and resistance exercise training course. To assess the effects of supplementation on physical performance, maximal oxygen consumption ($VO_2max$), anaerobic threshold (AT), lactic acid, and muscle strength of the dominant knee were measured at baseline, every visit, and after the training program. Results: Both ginsenoside groups showed significant increases in $VO_2max$ and muscular strength during exercise training. There were no definite changes in AT and lactic acid levels over time. After exercise training, there were definite differences in the $VO_2max$ (28.64.9 to $33.7{\pm}4.9ml/kg/min$ in high-dose group vs. $30.4{\pm}6.7$ to $32.8{\pm}6.6ml/kg/min$ in placebo, p = 0.029) and AT ($19.3{\pm}4.2$ to $20.9{\pm}3.5ml/kg/min$ in high-dose group vs. $20.0{\pm}5.1$ to $20.0{\pm}4.9ml/kg/min$ in placebo, p = 0.038) between the high-dose ginsenoside and placebo groups. However, there was no difference in $VO_2max$ between the low-dose ginsenoside and placebo groups (p = 0.254). There were no differences in muscular strength during exercise training among the three groups. Conclusion: High-dose ginsenoside supplementation (UG0712) augmented the improvement of aerobic capacity by exercise training.

• Journal of Ginseng Research
• /
• 제42권2호
• /
• pp.133-143
• /
• 2018

Background: AD-2 (20(R)-dammarane-3b, 12b, 20, 25-tetrol; 25-OH-PPD) is a ginsenoside and isolated from Panax ginseng, showing anticancer activity against extensive human cancer cell lines. In this study, effects and mechanisms of 1C ((20R)-3b-O-(L-alanyl)-dammarane-12b, 20, 25-triol), a modified version of AD-2, were evaluated for its development as a novel anticancer drug. Methods: MTT assay was performed to evaluate cell cytotoxic activity. Cell cycle and levels of reactive oxygen species (ROS) were determined using flow cytometry analysis. Western blotting was employed to analyze signaling pathways. Results: 1C concentration-dependently reduces prostate cancer cell viability without affecting normal human gastric epithelial cell line-1 viability. In LNCaP prostate cancer cells, 1C triggered apoptosis via Bcl-2 family-mediated mitochondria pathway, downregulated expression of mouse double minute 2, upregulated expression of p53 and stimulated ROS production. ROS scavenger, N-acetylcysteine, can attenuate 1C-induced apoptosis. 1C also inhibited the proliferation of LNCaP cells through inhibition on $Wnt/{\beta}-catenin$ signaling pathway. Conclusion: 1C shows obvious anticancer activity based on inducing cell apoptosis by Bcl-2 family-mediated mitochondria pathway and ROS production, inhibiting $Wnt/{\beta}-catenin$ signaling pathway. These findings demonstrate that 1C may provide leads as a potential agent for cancer therapy.

• 한국융합학회논문지
• /
• 제8권10호
• /
• pp.385-395
• /
• 2017

본 연구는 남한에 거주하고 있는 북한이탈여성의 일과 자녀돌봄 양립 경험을 총체적으로 이해하고 분석하고자 실시되었다. 연구자는 서울 및 수도권에 거주하고 있는 맞벌이 가정의 북한이탈여성 5명을 선택하고 심층면접을 실시하여 자료를 수집하였다. 자료는 현상학적 질적연구 방법으로 분석하여 구성요소 5개와 하위구성요소 14개가 도출되었다. 연구결과 <북한에서의 성장 배경>을 가진 연구참여자들은 <탈북과정에서의 가족 해체 및 재구성>의 복잡한 과정을 거치면서 남한에 입국했다. <남한 정착과정에서 자녀돌봄의 어려움>과 <진로 선택과 취업 지속의 어려움>사이에서 고군분투했으며 그와 관련하여 <조화로운 일-자녀돌봄의 양립을 위한 제안>을 제시하였다. 본 연구의 결과를 토대로 북한이탈여성의 일과 자녀돌봄을 균형있게 양립할 수 있는 실천적 서비스를 위한 제안으로서 부모교육, 경력경로 형성 프로그램, 성인지적 관점의 정책개발을 제시하였다. 또한 연구의 한계와 후속연구에 대한 제언을 제공하였다.

• 대한가정학회지
• /
• 제37권1호
• /
• pp.133-143
• /
• 1999

Originally, ancestral rites is a ceremony to pay filial piety to ancestors continuously even though they died, and this is a startingpoint based on the thought of paying respect to ancestors and 'root consciousness' which Korean people have. In the earliest days, Korean people worshipped nature and the sacrificial rituals were performed mainly for the fods of the skies and the earth. After the end-Koryeo Era introduced [Juja-Garye]ancestrial rites of forefather in home generalized. By the way, the foods for ancestrial rites on the original literature centering the [Sa-Rye-Pyon-Ram]are as follows: raw meat(saeng: 牲), rice and broth(ban, gaeng: 飯羹), fruits(果), slice meats(po: 脯), salted dry fish(jaban(佐飯), rice punch(sikhye: 食醯), soup(tang: 湯), fish and meat, roasts beef (jeok: 炙), roast vegetable(sookchae: 熟菜), rice cake(pyon: 餠), noodles(麵), soy(醬), Kimchi(沈菜), drink(酒), green tea(cha: 茶) etc. Today, ancestral rites, basically with this conception, has a function to bind the family and relatives as one. But as double income family are increasing, most housewives have heavy burden mentally and financially to prepare the food for the ancestral. The foods for ancestral rites can be said a way to express the internal true heart, and a basic medium to practice the filial piety. Many documents let us know that a few days before the ancestral rites, we should prepare the food for it with careful and pious attitude. And, they stress that our sincere attitude in preparing food is important rather than its quantity. In this industrial society, we have lots of difficulty preparing and observing the same service as it in the traditional socity. But I think that housewives can be freed from the burden they have to some degree when they realize what the true meaning of ancestral rites is and that the food for it plays a role an external expression.

• 한국가족복지학
• /
• 제57호
• /
• pp.39-67
• /
• 2017

본 연구는 한부모 이주여성의 삶의 의미와 본질을 자녀양육 경험을 중심으로 깊이 있게 살펴보는 것을 목적으로 한다. 이를 위해 한부모 이주여성 7명을 연구 참여자로 선정하여, 2015년12월부터 약 2개월간 일대일 심층면접을 진행하였다. 자료 분석은 연구 참여자의 고유하고 독특한 경험의 본질을 파악하기 위해 Giorgi의 현상학적 연구 방법을 적용했다. 분석 결과, 201 개의 의미단위, 29개의 하위 구성요소 및 13개의 구성요소를 도출했으며 이는 '기대와 달리힘들고 어려운 결혼 생활', '내면의 상처 때문에 자녀와 갈등', '이주여성이자 한부모로서 겪어내야 하는 이중고', '견디고 이겨내며 가장이 되어감', '어머니의 능동적 사랑과 그 원동력인자녀'의 5개 본질적 주제로 구조화되었다. 연구를 통해 파악한 한부모 이주여성의 삶과 경험의본질을 바탕으로 관련 정책의 개선 및 사회복지 실천방안을 제언하였다.

• The Plant Pathology Journal
• /
• 제37권2호
• /
• pp.162-172
• /
• 2021

Soybean mosaic virus (SMV) is the predominant viral pathogen that affects the yield and quality of soybean. The natural host range for SMV is very narrow, and generally limited to Leguminosae. However, we found that SMV can naturally infect Pinellia ternata and Atractylodes macrocephala. In order to clarify the molecular mechanisms underlying the cross-family infection of SMV, we used double-stranded RNA extraction, rapid amplification of cDNA ends polymerase chain reaction and Gibson assembly techniques to carry out SMV full-length genome amplification from susceptible soybeans and constructed an infectious cDNA clone for SMV. The genome of the SMV Shanxi isolate (SMV-SX) consists of 9,587 nt and encodes a polyprotein consisting of 3,067 aa. SMV-SX and SMV-XFQ008 had the highest nucleotide and amino acid sequence identities of 97.03% and 98.50%, respectively. A phylogenetic tree indicated that SMV-SX and SMV-XFQ018 were clustered together, sharing the closest relationship. We then constructed a pSMV-SX infectious cDNA clone by Gibson assembly technology and used this clone to inoculate soybean and Ailanthus altissima; the symptoms of these hosts were similar to those caused by the virus isolated from natural infected plant tissue. This method of construction not only makes up for the time-consuming and laborious defect of traditional methods used to construct infectious cDNA clones, but also avoids the toxicity of the Potyvirus special sequence to Escherichia coli, thus providing a useful cloning strategy for the construction of infectious cDNA clones for other viruses and laying down a foundation for the further investigation of SMV cross-family infection mechanisms.

• 한일민족문제연구
• /
• 제32호
• /
• pp.215-275
• /
• 2017

In the absence of the alternative public space of women in Japan, the experience of the "Bongseonhwa" was interpreted as the public domain of Japanese society as a public domain, a confession that focused on gender discrimination in the patriarchal system of Japan, Most of the enemy discourse is. These alienated discourses are the product of the efforts of women in Japan who do not want to forget about the traces and memories that can not be incorporated into the big narrative. It can not be denied that the women in the society of Japan have been excessively excluded and alienated by national ideology and patriarchal ideology. The meaning of presenting them through "Bongsinghwa" is the resistance of the minority, and it is the expression way of reconstructing and strengthening the identity of the women, and it is said to be a space of symbolic meaning. It is further clarified that it is based on a narrative that creates a new life area for coexistence with Japanese society, on the other hand, by constantly searching for the linkage with the motherland, held by women in Japan. As a result, between public social phenomena and private living space, confirmed that it conflicts with repetitive internal contradiction of controlling power and confirmed that complicated and detailed material of women living in Japan who undergo double discrimination What has been expressed over a period is considered to be a resistance expression and a will of expression of reconciliation to coexist with Japanese society. I have attempted to analyze the confessed alienated discourse of "Bongsinghwa" by classifying it as . As a result, it is confirmed that the public social phenomenon and the private life space are confronted with the repetitive internal contradictions of the power of domination, and the expression of the complex and detailed material of the discriminated women in Japan over a long period of time is a resistance to symbiosis with Japanese society And the will of the conversation.

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권7호
• /
• pp.928-935
• /
• 2008

The myostatin gene of seven important meat (Beltex (Australia), Beltex$\times$Huyang (F1), Meat and Multi-Prolific Chinese Merino Fine Wool, Meat Chinese Merino Fine Wool and Dorper (South Africa)) and non-meat (Huyang and Kazak) sheep breeds was analyzed to study the genetic basis of muscular hypertrophy (double muscling) phenotype in sheep. SNPs, four in regulatory regions and several in the introns in the myostatin gene, were identified, and the former four SNPs were used for further studies. Twelve haplotypes were predicted by PHASE program, of which four main haplotypes (1, 3, 7, 9) were present in 90% of the 364 sheep in the study. Haplotypes 1-4 were mainly present in meat breeds while haplotypes 7 and 9 dominated the non-meat breeds. The association between haplotypes and average daily gain (ADG) was analyzed among 116 sheep with production data, Haplo2 (CGAA) and Haplo8 (TGAA) were identified to have significant (p<0.05) effect on ADG by the model (JMP5.1 software) taking into account the effects of breed, family background, haplotype, birth weight and sex. ADG of these haplotype groups also correlated well (r = 0.82) with hypertrophic phenotype scores. In conclusion, the mutations -956 (T$\rightarrow$C), -41 (C$\rightarrow$A) and 6223 (G$\rightarrow$A) involved in Haplo2 and 8 may be associated with the double-muscling trait by influencing myostatin function and be suitable markers in selecting meat sheep.