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The Study of Application of Bio-Surfactant Producing Bacteria for Growing Crop in Oil Spilled Soil (기름으로 오염된 토양에서 작물생육을 위한 계면활성제 생산 Bacteria의 활용에 관한 연구)

  • Hwang, Cher-Won;Chang, Hae-Won;Choe, Yong-Rak
    • Journal of Life Science
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    • v.17 no.7 s.87
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    • pp.944-947
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    • 2007
  • Bacillus sp.LPO3 (producing emulsifying substances such as bio-surfactant) was used as a bio-control agent to degrade hydrocarbon (gasoline in oil spilled crop soil). The soil (brought from fertilizer store)was mixed with gasoline-spilled soil (made with Diatomaceous Earth, Sigma.U.S.A). The study was conducted for a period of 13 days, 13 days during which bacterial growth, hydrocarbon degradation and growth parameters of Bacillus sp.LP03 including shoot and root length were studied. We found that the effective of bacterial producing substance might bio-surfactants let the plants survive even more promote the growth of shoot and root length and showed antifungal activity against gray mold. Without the bacteria, they couldn't grow in oil-spilled soil not even survive. According to the results of the above experiments, we can see with following results, hydrocarbon in gasoline was reduced, day by day, then RNA dot blotting was done and it fit the results we had done. Finally, this Bacteria(producing bio-surfactant) were found to have effective bio-control agent for cropping in oil spilled soil and infected by gray mold.

An Improvement of User Interface for Design Idea Generation System based on WEB2.0 (WEB2.0 기반 디자인 아이디어 발상 시스템의 사용자 인터페이스 개선)

  • Choi, Eun-Suk;Chung, Seung-Ho;Kim, Dea-Yong
    • The Journal of the Korea Contents Association
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    • v.10 no.1
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    • pp.37-45
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    • 2010
  • WEB or Internet has given mankind an unprecedented experience in indefinite sharing of online content by original means of digitalized information, but it comes to face an empirical issue of unreasonable informational superfluity as well as a practical issue of collapsed dot.com bubble economy in 2001. Instead, a latest networking concept called 'WEB 2.0' or 'Semantic WEB' becomes embodied as a new approach to entities such as end users and content. The concept of WEB 2.0 for creating a platform on the basis of openness and collaboration has made such a technological setting that we can effectively resolve and manage unreasonable data maintenance and interface inherent in Creative Group Thinking System (CGTS), a WEB-based computer-aided idea generation system developed in 2003. Concerning decreased usability and difficulties with data maintenance due to certain issues of CGTS as a part of WEB R&D platform, such as complex display composition and inefficient data processing system, this study seeks to simplify and streamline data structure by means of AJAX and DOM as WEB2.0-based technologies, and integrate interface structure of WEB platform to focus on end users, so that it can improve interface of conventional CGTS for the purpose of realizing end user's participation through improving usability.

Stabilization of Pb Contaminated Army Firing Range Soil using Calcined Waste Oyster Shells (소성가공 굴껍질을 이용한 군부대 사격장내 고농도 납 오염토양의 안정화)

  • Moon, Deok-Hyun;Cheong, Kyung-Hoon;Kim, Tae-Sung;Khim, Jee-Hyeong;Choi, Su-Bin;Ok, Yong-Sik;Moon, Ok-Ran
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.2
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    • pp.185-192
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    • 2010
  • The objective of this study was to investigate the effectiveness of stabilization for army firing range soil highly contaminated with Pb (total Pb: 29,000 mg/kg) using calcined waste oyster shells. The calcination was conducted to activate quicklime from calcite. In order to evaluate the effectiveness of calcination, both natural oyster shells (NOS) and calcined oyster shells (COS) were applied to the Pb contaminated soil. Stabilization was conducted by mixing the contaminated soil with oyster shell media at 5-20 wt% and cured for 28 days. Following 28 days of curing, Pb leachability was measured based on the Korean Standard Test method (0.1 N HCl extraction). The treatment results showed that the COS treatment outperformed the NOS treatment. All of the NOS treatments failed to meet the Korean warning standard of 100 mg/kg. However, the Pb concentrations were significantly reduced to 47 mg/kg and 3 mg/kg upon 15 wt% and 20 wt% COS treatments, respectively which passed the Korean warning standard. Moreover, -#20 mesh materials were more effective than the -#10 mesh materials in effectively reducing Pb leachability. The scanning electron microscopy (SEM)-energy dispersive X-ray spectroscopy (EDX) results indicated that Pb immobilization was strongly linked to Al and Si.

Evaluation of Flow Experience by using Psychophysiological Visual Feedbacks

  • Kim, Jung Yong;Min, Seung Nam;Park, Yong Duck
    • Journal of the Ergonomics Society of Korea
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    • v.32 no.6
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    • pp.481-487
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    • 2013
  • Objective: The present study aims to evaluate the visual reactions of users when they are playing games of different flow levels, and to explore the visual variables that can sensitively reflect the different flow levels. Background: The flow is defined as a psychological state where interface users feel their actions in a virtual setting identical to those in real environment. To measure the flow states of users, the questionnaire-based FSS(Flow State Scale) has mostly been used. However, this method is a qualitative test that has limits in terms of the accuracy of users' flow experiences. Therefore, more accurate methods to measure users' flow experiences are required. Method: Ten subjects participated in the experiment, where the independent variables were three games with different flow levels(puzzle games, dot drawing and coloring) and the time frame(the first and last 10 seconds in game playing), whereas the dependent variables included the pupil size and the frequency and duration of eye blinking. This study was a within-subject design. Each participant performed three types of games with different flow levels 3 times for each for 10 minutes, and their visual reactions to each game were measured. Results: The higher the flow cause the bigger pupil size(p<0.01) and the lower eye blinking frequency(p<0.1), indicating that different types of games lead to different flow levels. The pupil size during the last 10 seconds when the flow level was higher was bigger by 2.1% compared with that during the first 10 seconds in game playing(p<0.1), and the eye blinking frequency decreased by 12%(p<0.01). Conclusion: It was found out that the pupil size and the frequency and duration of eye blinking were psychophysiological indices for evaluating users' flow experiences, which could quantify the flow states users go through. The psychophysiological variables capable of measuring diverse aspects of the flow need diversifying to be applicable to precise measurement of the flow. Application: These studies are warranted for both quantitative analysis of flow levels and qualitative improvement of cyber leisure in line with development of healthy games.

Syntheses and Properties of ZnS:Mn/ZnS Core-Shell Quantum Dots Prepared via Thermal Decomposition Reactions of Organometallic Precursors at Various Reaction Temperatures (다양한 온도 조건에서의 ZnS:Mn/ZnS 코어-쉘 양자점의 합성 및 광 특성에 관한 연구)

  • Lee, Jae-Woog;Hwang, Cheong-Soo
    • Journal of the Korean Chemical Society
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    • v.53 no.6
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    • pp.677-682
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    • 2009
  • ZnS:Mn/ZnS core-shell quantum dots (QDs), were synthesized via a thermal decomposition reaction of organometallic precursors in a hot solvent mixture. The synthetic conditions of the quantum dots were monitored at various reaction temperatures for the core formation, while the shell formation temperature was fixed at 135$^{\circ}C$. The obtained colloidal nanocrystals at corresponding temperatures were characterized by UV-Vis, solution photoluminescence (PL) spectroscopies, and further obtained powders were characterized by XRD, HR-TEM, and EDXS analyses. The synthetic temperature condition to obtain the best PL emission intensity for the core-shell QD was 135$^{\circ}C$, for both core and shell formation. At this temperature, solution PL spectrum showed a narrow emission peak at 583 nm with a relative PL quantum efficiency of 42.15%. In addition, the measured spherical particle sizes for the ZnS:Mn/ZnS nanocrystals via HR-TEM were in the range of 4.0 to 5.4 nm, while ellipsoidal particles were obtained at 150$^{\circ}C$.

Relationships between Selective Attention Bias for Fear Stimuli and Hallucination in Patients with Schizophrenia : A Preliminary Study (조현병 환자에서 불안자극에 대한 선택적 주의 편향과 환청과의 연관성 : 예비 연구)

  • Kim, Han-Suk;Han, Jin-Hee;Hong, Seung-Chul;Jeong, Jong-Hyun;Lim, Hyun-Kook;Kim, Tae-Won;Um, Yoo-Hyun;Chae, Jeong-Ho;Lee, Kyoung-Uk;Seo, Ho-Jun
    • Anxiety and mood
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    • v.12 no.1
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    • pp.7-12
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    • 2016
  • Objective : This study was conducted to evaluate the relationships between selective attention bias for fear stimuli and hallucination in patients with schizophrenia Method : A total of 66 patients with schizophrenia admitted to psychiatry clinics were included in the study. Selective attention bias was measured by the dot-probe task. Patient symptoms were measured using the Positive and Negative Symptom Scale, Psychotic Symptom Rating Scale (PSYRATS), Korean version of the Scale to Assess Unawareness of Mental Disorder, and Clinical Global Impression-Severity scale. Results : Selective attention bias was correlated with the hallucination subscale of PSYTATS (r=0.268, p=0.029). No correlation was found between selective attention bias and other clinical measures. There was no significant difference, but a statistical trend was found (p=0.092) in hallucination severities between the biased and non-biased groups. Conclusion : The results suggest that selective attention bias for fear stimuli is associated with auditory hallucination. This preliminary study suggests the possibility of correlation between auditory hallucination in the psychotic domain and anxiety of the affective component.

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Morphological Analyses of Natural Populations of Sedum kamtschaticum (Crassulaceae) and the Investigation of Their Vegetations (기린초(Sedum kamtschaticum Fisch.)의 자생지별 외부형태분석과 식생연구)

  • Ryu, Hye-Seon;Jeong, Ji-Hyeon;Kim, Sang-Tae;Paik, Weon-Ki
    • Korean Journal of Plant Resources
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    • v.24 no.4
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    • pp.370-378
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    • 2011
  • To address infra-specific relationships of Sedum kamtschaticum Fisch., and to provide the fundamental information for developing new horticultural variations, we analyzed the morphology of individuals in four natural populations (Mt. Gwangdeok, Mt. Samyeong, Mt. Yonghwa, Tongyeong) and investigated the vegetations of these area. Based on 50 morphological characters the principle component analysis (PCA) has been performed. Principle component axis 1, 2, and 3 explain 22.9%, 14.2%, and 7.4% of total variations, respectively. Dot plot of OTUs in PC2 by PC1 area showed that the areas of four populations are completely overlapped. The result of PCA and the statistics of each character indicate that all of morphological characters are overlapped in these four populations. The maximum deviations are found in the characters related in the size and shape of the leaf. In the vegetation analyses, eighteen community plots that we investigated were grouped into 10 subcommunities: subcomm. Boehmeria spicata, subcomm. Artemisia stolonifera, subcomm. Artemisia keiskeana, subcomm. Impatiens nolitangere, subcomm. Crepidiastrum chelidoniifolium, subcomm. Urtica thunbergiana, subcomm. Artemisia gmelini, subcomm. Commelina communis, subcomm. Erigeron annuus-Artemisia princeps, and typical subcommunity.

Expression Patterns of Bacillus subtilis Diacylglycerol Kinase Gene Induced by Physiological Stimuli (Bacillus subtilis dgk (diacylglycerol kinase) 유전자의 생리적 자극에 의한 유도발현)

  • Lee, Mi-Young;Suh, Seok-Jong;Lee, Jin-Hyung;Song, Bang-Ho;Kim, Jong-Cuk
    • Microbiology and Biotechnology Letters
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    • v.30 no.1
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    • pp.15-20
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    • 2002
  • Diacylglycerol kinase (DGK) phosphorylates the second messenger diacylglycerol (DAG) to phosphatidic acid and it may play a role in signal transduction in Escherichia coli as well as in eukaryotic cells. In addition, DGK is important for microorganisms to adapt to several physiological stimuli. In Bacillus subtilis, the effect of stress on dgk transcription was examined by northern hybridization. The high level of dgk transcription was induced against high osmolarity, low pH value and low temperature. Transcriptional analysis revealed that the dgk gene and dgk upstream locus (ORF2, ORF3 and ORF4) were transcribed as a polycistronic mRNA to form an approximately 2.5 kb transcript.

The differences in Constellation drawings among different countries

  • Karimova, Ulkar;Yi, Yu;Oh, Suyeon
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.95-95
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    • 2012
  • Constellations are formed of bright stars which appear close to each other on the sky, but are really far apart in space. The shapes you see all depend on your point of view. Back before people had televisions and electricity to light their homes at night, they spent a lot more time looking at the stars. People all over the world used their imaginations to draw pictures in the sky, as if it were a giant connect-the-dot game. The patterns they imagined are called constellations. People usually saw patterns that reflected their different cultures. Native Americans in North America imagined many animals and shapes from the natural world. The ancient Greeks found images of gods and goddesses in the stars. Sometimes people from very different parts of the world even imagined the same animal or shape in the same stars. Most of the constellations we recognize today were made up by the ancient Greeks around 6,000 years ago. Different constellations are visible at different times of year, so the first appearance of these patterns told farmers of the changing seasons and reminded them to plant or harvest their crops. The constellations also help us to find our way around the night sky and to remember which stars are which. The star names we use today are mostly from Greek and Arabic, but many are changed a bit from the original, as often happens when words are passed from one language to another. It can be difficult to picture just what those folks long ago were seeing in the stars, so don't be discouraged if you have trouble seeing their patterns. You can even make up your own! In ancient world all the countries or regions had their own way to name things up in the sky, make up stories and draw different shapes for constellations. Today there are 88 official constellations, but you may find that different books show their stars connected in slightly different ways. The official constellations are specific regions of the sky, so the exact patterns are not all that important. However in various cultures there are some famous star patterns that use stars from only apart of a constellation, or even connect stars from different constellations. These patterns of stars that are not official constellations are called asterisms. The Big Dipper is a very famous asterism, found in the constellation Ursa Major, or Great Bear.

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Photoemission Electron Micro-spectroscopic Study of the Conductive Layer of a CVD Diamond (001)$2{\times}1$ Surface

  • Kono, S.;Saitou, T.;Kawata, H.;Goto, T.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.7-8
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    • 2010
  • The surface conductive layer (SCL) of chemical vapor deposition (CVD) diamonds has attracting much interest. However, neither photoemission electron microscopic (PEEM) nor micro-spectroscopic (PEEMS) information is available so far. Since SCL retains in an ultra-high vacuum (UHV) condition, PEEM or PEEMS study will give an insight of SCL, which is the subject of the present study. The sample was made on a Ib-type HTHP diamond (001) substrate by non-doping CVD growthin a DC-plasma deposition chamber. The SCL properties of the sample in air were; a few tens K/Sq. in sheet resistance, ${\sim}180\;cm^2/vs$ in Hall mobility, ${\sim}2{\times}10^{12}/cm^2$ in carrier concentration. The root-square-mean surface roughness (Rq) of the sample was ~0.2nm as checked by AFM. A $2{\times}1$ LEED pattern and a sheet resistance of several hundreds K/Sq. in UHV were checked in a UHV chamber with an in-situ resist-meter [1]. The sample was then installed in a commercial PEEM/S apparatus (Omicron FOCUS IS-PEEM) which was composed of electro-static-lens optics together with an electron energy-analyzer. The presence of SCL was regularly monitored by measuring resistance between two electrodes (colloidal graphite) pasted on the two ends of sample surface. Figure 1 shows two PEEM images of a same area of the sample; a) is excited with a Hg-lamp and b) with a Xe-lamp. The maximum photon energy of the Hg-lamp is ~4.9 eV which is smaller that the band gap energy ($E_G=5.5\;eV$) of diamond and the maximum photon energy of the Xe-lamp is ~6.2 eV which is larger than $E_G$. The image that appear with the Hg-lamp can be due to photo-excitation to unoccupied states of the hydrogen-terminated negative electron affinity (NEA) diamond surface [2]. Secondary electron energy distribution of the white background of Figs.1a) and b) indeed shows that the whole surface is NEA except a large black dot on the upper center. However, Figs.1a) and 1b) show several features that are qualitatively different from each other. Some of the differences are the followings: the two main dark lines A and B in Fig.1b) are not at all obvious and the white lines B and C in Fig.1b) appear to be dark lines in Fig.1a). A PEEMS analysis of secondary electron energy distribution showed that all of the features A-D have negative electron affinity with marginal differences among them. These differences can be attributed to differences in the details of energy band bending underneath the surface present in SCL [3].

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