• Title/Summary/Keyword: dormancy breaking

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Physiological and Ecological Studies on the Seed Dormancy of Dominant Weed Species in Korea (우리나라 우점(優占) 잡초종(雜草種)의 휴면(休眠)에 관한 생리(生理) 생태학적(生態學的) 연구(硏究))

  • Kang, Byeung-Hoa;Shim, Sang-In;Lee, Sang-Gak;Shin, Hyeun-Won
    • Korean Journal of Environmental Agriculture
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    • v.12 no.2
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    • pp.193-207
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    • 1993
  • The seed dormancy of weed species is the important mechanisms to unfavorable conditions but it brings about critical problems in weed control. The factors which induced dormancy were varied with species and their physiological conditions. More than 20 of 50 species of dominant weed species showed the seed dormancy. When several physical treatments were given to seeds to break the dormancy, each species showed the different responses. The germination percentage and germination velocity were increased with alternating temperature. The treatment of more than 4 weeks of stratification had strong effect on dormancy breaking. The ${\alpha}-amylase$ activities of germinating seeds were increased in proportional to the period of stratification treatment of dormant seeds. The contents of soluble protein and soluble sugar were changed slightly with stratification.

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Dormancy and Germination Characteristics of Round-Leaved Sundew (Drosera rotundifolia L.) Seeds Native to Korea (자생 끈끈이주걱(Drosera rotundifolia L.) 종자의 휴면과 발아특성)

  • Cho, Ju Sung;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.29 no.5
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    • pp.564-573
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    • 2016
  • This research was performed to develop mass propagation method of round-leaved sundew (Drosera rotundifolia L.) for improving horticultural usefulness. Seeds were collected around Hwaseong-si, Gyeonggi-do in October of 2011 and experimented upon while being dry-stored at 4 ± 1.0℃ (darkness). Seed length of a major and minor axis were 1.58 ± 0.060 and 0.21 ± 0.016 ㎜, respecrively, and weight of 1,000 seeds was 6.24 ± 0.172 ㎎. Seeds were thus classified as ‘dwarf seeds’. Regarding the dormancy type, since round-leaved sundew seeds were dormancy broken and germinated at 20~30℃ under the light condition after wet-chilling treatment for 12 weeks, seeds were estimated to have physiological dormancy. Germination conditions of dormancy broken seeds were found to be 20℃ and light condition (54.7%), but germination decreased at higher temperature. Percent germination (PG), germination energy (GE), mean germination time (MGT) and T50 were effectively improved by chemical treatment such as GA3 200 ㎎/L + kinetin 20 ㎎/L and wet-chilling treatment for 14 weeks. In conclusion these optimal conditions were thought to be a useful method for raising seedling uniformly.

Optimum Chilling Terms for Germination of the Dehisced Ginseng(Panax ginseng C. A. Meyer) Seed (개갑 인삼종자의 발아 적정 저온감응기간)

  • 권우생;이장호;이명구
    • Journal of Ginseng Research
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    • v.25 no.4
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    • pp.167-170
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    • 2001
  • Experiments were conducted to study the optimum chilling period for breaking physiological dormancy of dehisced ginseng(Panax ginseng C. A. Meyer) seed. Embryo of ginseng seed is too small to be noticed with naked eyed on harvesting time. Embryo grew to half size of endosperm after seeds were stratified for 3 months. It takes 6 months for this embryo to reach the size enough for germination. And it grew faster indoors than outdoors. Dehisced ginseng seed with full-size embryo did not germinate at room temperature and required chilling treatment for 75days in outdoors and 90 days in cold chamber. While seed receiving sufficient chilling treatment were left to be in room temperature, the chilling effect decreased remarkably.

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Gibberellin Effects on Inflorescence Development, Bud Dormancy and Root Development in North American Ginseng

  • Rolston, L.J.;Proctor, J.T.A.;Fletcher, R.A.;Murr, D.P.
    • Journal of Ginseng Research
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    • v.26 no.1
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    • pp.17-23
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    • 2002
  • Gibberellic acid (GA) was applied to field-grown 3-year-old North American ginseng (Panax quinqueiolius L.) between 1 and 4 times, before and during bloom in 1999. Applications of both GA$_3$ and GA$\sub$4+7/ four times (x4) to the developing inflorescences increased maximum pedicel length, and seed head diameter and height. Treatment with GA$\sub$4+7/ increased mean and total root fresh weight linearly, whereas those treated with GA$_3$ did not show similar increases. Both GA$_3$ and GA$\sub$4+7/ at 50, 100 and 200 mg L$\^$-1/ (x4) increased the incidence of breaking of dormancy of perennating buds with GA$_3$ being twice as effective as GA$\sub$4+7/. Both GA$_3$ and GA$\sub$4+7/ treatments resulted in an increased number of new bud initials forming per root, with the number of new initials per root increased two-fold by the GA$_3$ sprays compared to GA$\sub$4+7/.

Effects of Gibberellic Acid and Alternating Temperature on Breaking Seed Dormancy of Panax ginseng C. A. Meyer (개갑된 인삼종자 휴면 조기타파에 미치는 GA3 및 변온처리 효과)

  • Lee, Jung Woo;Kim, Young Chang;Kim, Jang Uk;Jo, Ick Hyun;Kim, Kee Hong;Kim, Dong Hwi
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.4
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    • pp.284-293
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    • 2016
  • Background: Developing new ginseng cultivars is a significant time-consuming process owing to the three years of growth required for ginseng to flower. To shorten the ginseng breeding process, it is necessary to establish rapid progression through each generation. In this study, we examined it was possible to rapidly break ginseng seed dormancy using gibberellic acid ($GA_3$) treatment and alternating temperature. Methods and Results: Seeds were obtained from local variety. Seeds were treated with either $GA_3$ at a concentration of $100mg/{\ell} $, constant temperature ($-2^{\circ}C$ and $2^{\circ}C$), alternating temperature ($2^{\circ}C$ followed by $-2^{\circ}C$, followed by $2^{\circ}C$) or a combination $GA_3$ and temperature treatment. Following experimental treatment, seeds were sown into trays and placed in a greenhouse. Low germination rates were observed in seeds that did not receive $GA_3$ treatment, which were similar following $2^{\circ}C$ and $-2^{\circ}C$ constant temperature treatment. Germination rates increased in proportion to $GA_3$ and more so when combined with alternating temperature treatment. In additon, stem and leaf lengths of the resulting ginseng plants were increased following $GA_3$ treatment, although no synergistic effect was observed with alternating temperature treatment. Conclusions: These results suggest that a combination $GA_3$ and alternating temperature treatment enhances ginseng seed germination, which can contribute to shortening the time required to progress through a single ginseng generation for breeding.

Effect of Low Dose ${\gamma}$ Radiation on the Dormancy Breaking and Physiological Activity of "Dejima" Seed Potato (Solanum tuberosum L.) (씨감자 “대지” 품종의 휴면타파와 생리활성에 미치는 저선량 방사선 효과)

  • Kim, Jae-Sung;Kim, Dong-Hee;Back, Myung-Hwa;Joung, Hyouk;Lee, Young-Bok
    • Korean Journal of Environmental Agriculture
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    • v.20 no.2
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    • pp.116-121
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    • 2001
  • To observe the stimulating effects of low dose ${\gamma}$ - radiation on the dormancy breaking and physiological activity, potato (Solanum tuberosum L. cv. Dejima) were irradiated at the dose of $0.5{\sim}30$ Gy. Low dose ${\gamma}$ - radiation exhibited promoting effects on the sprouting rate within the range of optimum dose of 1 Gy and 2 Gy and on the sprout length within the range of optimum dose of 2 Gy and 4 Gy. Regardless of storage duration and irradiation doses, the number of sprouts was enhanced. The number of sprouts, which were reserved for 15 days after production (DAP), were significantly increased under 4, 8 and 16 Gy irradiation. The growth of 45 DAP sprouts was extremely stimulated under 4 Gy irradiation accompanying the increase of peroxidase activity in the plantlet. In this study, it was also suggested that the activities of antioxidative enzymes of potato plantlets were not related to the irradiation dose during the plantlet development with the exception of decrease in catalase activity.

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Effects of Pre-sowing Seed Soaking and Planting Depth on Dormancy Breaking and Seedling Emergence of Pearl Millet (Pennisetum americanum(L.) Leeke) (진주조의 파종전 침종 및 파종심도가 휴면타파와 출아에 미치는 영향)

  • Keun-Yong Park;Rae-Kyung Park;Byeong-Han Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.1
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    • pp.81-85
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    • 1989
  • One of the most important cultural techniques of pearl millet (Pennisetum americanum (L.) Leeke) is to encourage rapid and uniform emergence of seedlings to establish good stand and to let them grow well. Thus the objectives of the study were to investigate the effects of pre-sowing seed soaking and planting depth on dormancy breaking, germination and emergence of the seedlings, and to estimate the optimum planting season of pearl millet in Suwon, Korea. The seeds with dormancy germinated 99 to 100 percent when soaked in the H$_2$O$_2$ 1% solution for 24 hours and rinsed with pure water, but germinated only 38% and 83% when soaked in pure water for 24 hours just after harvest and drying, and one month later from the harvest time, respectively. The seeds of Australia inbred line did not germinate at the constant 10$^{\circ}C$, but germinated at the constant 11$^{\circ}C$. It also was possible to estimate the optimum planting season by applying minimum temperature 11$^{\circ}C$ for germination. The minimum air temperature reached from late April in Suwon, Korea in regular years but fluctuated from late April to early May in 1986 and 1987. Thus, the safe planting season was mid-May for rapid and uniform germination of pearl millet seed. The optimum depth of planting was 2∼4cm under the optimum soil moisture condition, and 4 to 6 cm under the drier soil moisture condition. Subcoleoptile internode(mesocotyle) length increased according to increased depth of planting. Seedling crown placement also became deeper due to deeper planting of the seeds. The subcoleoptile internode length and seedling crown depth were positively correlated with actual planting depth, indicating that deeper planting would be not good for appropriate adventitious root and tiller development.

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Seed Dormancy Type and Germination Characteristics in Tiarella polyphylla D. Don Native to Korea (한반도 자생식물 헐떡이풀 종자의 휴면유형과 발아특성)

  • Choi, Han;Lee, Seung Youn;Rhie, Yong Ha;Lee, Jeong Ho;Kim, Sang Yong;Lee, Ki Cheol
    • Korean Journal of Plant Resources
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    • v.31 no.4
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    • pp.363-371
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    • 2018
  • Tiarella polyphylla D. Don is a native plant distributed only in Ulleung Island in Korea and has been traditionally used for medicinal purposes, although it is also used ornamentally. This study was conducted to determine the requirements for dormancy break and germination and to classify the type of seed dormancy. The experiments were performed with cold stratification (0 or 12 weeks at $5^{\circ}C$), warm stratification (0, 4, 8, or 12 weeks at $23^{\circ}C$, followed by 8 weeks at $5^{\circ}C$, and then incubation at $23^{\circ}C$), and $GA_3$ treatments (0, 10, 100, or 1000 mg/L). The treated seeds were incubated on aseptic media at room chamber ($23^{\circ}C$, a 16h photoperiod of fluorescent lamps with $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). The seeds were dispersed in nature as underdeveloped embryos with no physical barrier to absorb water to prevent water absorption. However, the seeds did not germinate for 30 days after sowing without any pre-treatments. Thus, the seeds had morphological dormancy (MD) and physiological dormancy (PD). The final germination percentage following cold stratification (0 or 12 weeks) was 66.7% and 45.6%, respectively. The cold stratification delayed seed germination by about 3 weeks. In the warm stratification experiment (0, 4, 8, or 12 weeks), the final germination percentage was 21.1%, 27.8%, 41.1%, and 57.8%, respectively, 20 weeks after sowing. The embryos of the T. polyphylla seed grew in relatively warm temperatures ($23^{\circ}C$). $GA_3$ application overcame seed dormancy and promoted germination. Following $GA_3$ treatment (0, 10, 100, or 1000 mg/L), the final germination percentage was 33.3%, 45.0%, 42.5%, and 72.5%, respectively. These results suggest that the T. polyphylla seeds had non-deep simple morphophysiological dormancy (MPD) and $GA_3$ treatment could be used as a substitute for warm stratification for breaking seed dormancy. To our knowledge, this is the first report of seed dormancy characteristics of the genus Tiarella native to Korea.

Freezing Hardiness According to Dormancy Level and Low Temperature in Persimmon (Diospyros kaki) (감나무의 휴면정도 및 저온에 따른 내동성 비교)

  • Kim, Ho-Cheol;Bae, Kang-Soon;Bae, Jong-Hyang;Kim, Tae-Choon
    • Journal of Bio-Environment Control
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    • v.16 no.3
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    • pp.269-273
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    • 2007
  • Freezing hardiness of winter bud and branch according to dormancy level and low temperature, in persimmon (Diospyros kaki) cultivars, was investigated by electrolyte leaching rate, triphenyltetrazolium chloride (TTC) test, and sprouting. Electrolyte leaching rate was lowest in branch of 20th January and was highest in the 20th March. The electrolyte leaching rate of 'Fuyu' and 'Cheongdobansi' was high in the 20th January and was low in the 20th February, but 'Uenishiwase' and 'Nishimurawase' was opposed to that. 'Hachiva' was the middle level in the cultivars. Absence rate by TTC test was highest in the 20th January and was low in the others. The 20th March had a great decrease in $-10^{\circ}C$ treatment. The absence rate of 'Fuyu' and 'Uenishiwase' was low in the 20th January and March and was high in the 20th February. 'Nishimurawase' and 'Hachiya' had a high level irrespective of dormancy level. Sprouting was highest in the 20th February and was lowest in 20th March. Most cultivars were not sprout in $-20^{\circ}C$ treatment and 'Fuyu', 'Nishimurawase' and 'Cheongdobansi' was a little high level irrespective of dormancy level. 'Hachiya' was only high in the 20th January. Thus, freezing hardiness of persimmon was very weak low temperature after dormancy breaking and was not different between astringent and non-astringent persimmon.

Effects of Several Chemicals and Burial of Seeds into the Soil on Dormancy-breaking of Weed Species (잡초(雜草) 종자(種子)의 휴면타파(休眠打破)에 대한 화학물질(化學物質)과 토양(土壤) 내(內) 종자매몰(種子埋沒)의 효과(效果))

  • Shim, S.I.;Lee, S.G.;Kang, B.H.
    • Korean Journal of Weed Science
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    • v.18 no.4
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    • pp.295-303
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    • 1998
  • The seed dormancy is one of the peculiar characteristics of a number of weed species and it makes difficulties in weed control. To clarify the mechanism of seed dormancy, several chemicals such as $KNO_3$, KOH, thiourea, and $H_2O_2$ and phytohormone($GA_3$) were treated to dormant seeds. Among the species treated with several chemicals, the germination percentages of Setaria glauca, Ambrosia trifida and Ranunculus sceleratus were increased with $KNO_3$ and those of S. glauca, R sceleratus were increased with thiourea. Hydrogen peroxide promoted the germination of Setaria viridis and S. glauca. Germination percentages of S. viridis, S. glauca and Cyperus saraguinolentus were increased with enzyme treatment using pectinase. GA treatment enhanced the geim.ination of Eleusine indica and R sceleratus but the other species were affected slightly. Especially. E. indica showed linearity in the relationship between germination percentage and GA concentration. So, It seemed that E. indica can be used as a bioassy material for GA. Considering the phenological habits of weed species, the seeds were buried under soil for long time(more than 1 month) over winter. When seeds were buried in soil, the degree of dormancy was drastically decreased. Especially, germination of seeds buried were increased under alternating temperature. The germination rates of Persicaria ssp. and Chenopodium ssp. were increased by 50% order alternating temperature after burial for seven weeks.

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