• Animal Bioscience
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• v.35 no.1
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• pp.126-137
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• 2022

Objective: Efficient gene editing technology is critical for successful knock-in in domestic animals. RAD51 recombinase (RAD51) gene plays an important role in strand invasion during homologous recombination (HR) in mammals, and is regulated by checkpoint kinase 1 (CHK1) and CHK2 genes, which are upstream elements of RAD51 recombinase (RAD51). In addition, mismatch repair (MMR) system is inextricably linked to HR-related pathways and regulates HR via heteroduplex rejection. Thus, the aim of this study was to investigate whether clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9)-mediated knock-in efficiency of human lactoferrin (hLF) knock-in vector in the bovine β-casein gene locus can be increased by suppressing DNA MMR-related genes (MSH2, MSH3, MSH6, MLH1, and PMS2) and overexpressing DNA double-strand break (DSB) repair-related genes (RAD51, CHK1, CHK2). Methods: Bovine mammary epithelial (MAC-T) cells were transfected with a knock-in vector, RAD51, CHK1, or CHK2 overexpression vector and CRISPR/sgRNA expression vector to target the bovine β-casein gene locus, followed by treatment of the cells with CdCl₂ for 24 hours. After 3 days of CdCl₂ treatment, the knock-in efficiency was confirmed by polymerase chain reaction (PCR). The mRNA expression levels of DNA MMR-related and DNA DSB repair-related genes were assessed by quantitative real-time PCR (RT-qPCR). Results: Treatment with CdCl₂ decreased the mRNA expression of RAD51 and MMRrelated genes but did not increase the knock-in efficiency in MAC-T cells. Also, the overexpression of DNA DSB repair-related genes in MAC-T cells did not significantly affect the mRNA expression of MMR-related genes and failed to increase the knock-in efficiency. Conclusion: Treatment with CdCl₂ inhibited the mRNA levels of RAD51 and DNA MMR-related genes in MAC-T cells. However, the function of MMR pathway in relation to HR may differ in various cell types or species.

• Korean Journal of Environment and Ecology
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• v.36 no.2
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• pp.202-216
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• 2022

Animals exhibit certain behaviors and movement patterns as they react to their internal needs, external stimuli, and surrounding environments. They have a bounded range in which they mostly spend their time, and it is referred to as a home range. Based on the fact that the home range is a critical area for the survival and preservation of species, there has been a growing body of research on developing more precise home range estimation methods to use the estimated ranges as a ground for establishing an effective conservation policy since the early 1940s. Recent rapid advancements in telemetry technology that resulted in the presence of autocorrelation between locations with short time intervals revealed the limitations of the existing estimators. Many novel estimators have been developed to compensate for it by incorporating autocorrelation in calculating home ranges. However, studies on the animal home range are still in their early stage in Korea, and newly developed methodologies have not yet been adopted. Therefore, this study aims to introduce the foreign home range estimation methods and foster domestic research activities on home ranges. Firstly, we compared and contemplated seven estimators by categorizing them into geometrical and statistical methodologies and then divided them into estimators that assume independent observations and those that consider autocorrelation in each category. After that, the home ranges of black-tailed gulls (Larus crassirostris) were calculated using GPS tracking data for the month of June and derived home range estimators by applying the methodology introduced in this study. We analyzed and compared the results to discuss the strengths and weaknesses of each method. Lastly, we proposed a guideline that can help researchers choose an appropriate estimator for home range calculation based on the animal location data characteristics and analysis purpose.

• Journal of the Korean Wood Science and Technology
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• v.32 no.2
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• pp.82-89
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• 2004

Two kinds of chemicals (RNF-3 and RNF-4) with high anti-mold effectiveness against stored fungi causing fungal discoloration was selected from among the six kinds of fluoride-based chemicals in the preceding study. In this study, the anti-mold test using 16 species fungi isolated from the softwood lumbers which were fungal discolored and field test in the sawmill was carried out to prove the feasibility for practical using of selected chemicals.For the isolated fungi, the RNF-3 consist of F and Cu showed high mycelial growth control in the PDA medium and fungicidal effectiveness in the japanese red pine (Pinus densiflora), korean pine (Pinus koraiensis) and radiata pine (Pinus radiata) when the concentration was 2% or more. However the RNF-4 consist of F only was not effective compared with RNF-3 because of mycelial growth in the PDA medium and wood treated with 2% or more chemical solution. The RNF-3 also showed a strong anti-mold effectiveness because there was no fungal discoloration for the radiata pine boards treated by 2% and 10 min. soaking in the field test. These results mean that RNF-3 can be used as domestic anti-stain chemicals for prevention of fungal discoloration of the softwood lumber

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.1
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• pp.37-47
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• 2023

The black cutworm, Agrotis ipsilon (Hufnagel), is an important crop pest worldwide that feeds more than 80 plant species including cabbage, potato, maize, wheat and bean, and this moth is a typical pest attacking underground parts of crops. It has been known in farm booklets that the larvae of A. ipsilon overwinter in the soil in Korea, but no definitive data exist yet. This study was conducted to evaluate that the specific appearance time of A. ipsilon observed actually in the field could be explained when we assumed that this pest overwinters in a form of larvae or pupae. Degree day-based phenology models were applied for tracking forward or backward to find the predicted developmental stage which developed at a specific stage found in the field. As a result of the analysis, it was confirmed that an initial population could be established in a group that does not overwinter as larvae or pupae in Korea. In other words, the appearance of adults in early March to April could not be explained by the presence of domestic overwintering populations. Populations that overwinter as larvae or pupae in Korea were able to emerge as adults in June to July at the earliest. Therefore, the group of adults appearing in early spring is highly likely to be a population that migrated from outside Korea. Taken together, it was estimated that the colony of A. ipsilon in Korea would be formed by a mixture of a migrant population through long-distance migration and a overwintering population.

• Journal of Life Science
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• v.33 no.8
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• pp.605-611
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• 2023

Recently, sporadic cases of human infection by genetic reassortants of H7Nx influenza A viruses have been reported; such viruses have also been continuously isolated from avian species. In this study, A/wild bird/South Korea/sw-anu/2023, a novel reassortant of the H7N1 avian influenza virus, was analyzed using full-genome sequencing and molecular characterization. Phylogenetic analysis showed that A/wild bird/South Korea/sw-anu/2023 belonged to the Eurasian lineage of H7Nx viruses. The polymerase basic (PB)2, PB1, polymerase acidic (PA), and nucleoprotein (NP) genes of these viruses were found to be closely related to those of avian influenza viruses isolated from wild birds, while the hemagglutinin (HA), neuraminidase (NA), matrix (M), and nonstructural (NS) genes were similar to those of avian influenza viruses isolated from domestic ducks. In addition, A/wild bird/South Korea/sw-anu/2023 also had a high binding preference for avian-specific glycans in the solid-phase direct binding assay. These results suggest the presence of a new generation of H7N1 avian influenza viruses in wild birds and highlight the reassortment of avian influenza viruses found along the East Asian-Australasian flyway. Overall, H7Nx viruses circulate worldwide, and mutated H7N1 avian viruses may infect humans, which emphasizes the requirement for continued surveillance of the H7N1 avian influenza virus in wild birds and poultry.

• Journal of Korea Trade
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• v.27 no.2
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• pp.115-129
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• 2023

Purpose - This study empirically analyzes the consumer value of risk management associated with illegal, unreported, and unregulated (IUU) fishing of fishery products imported to Korea. The global regulatory paradigm for IUU fishing has shifted from production-centered to market-centered. As a result, changes in the international fisheries trade environment emphasizing "transparency" and "legality" of the production process are accelerating. Therefore, changes in the management systems of fishery products entering the country are also needed. Accordingly, this study estimated the consumer value for risk management of IUU fishing, targeting major fish species imported to Korea, and derived the feasibility of introducing related policies. Design/methodology - This study used the choice experiment as an analysis model to estimate consumers' willingness to pay for the "possibility to check for IUU fishing." The choice experiment assumes that the value of a good or service is composed of separable attributes and that the sum of the part-worth of these individual attributes becomes the total value. In this study, respondents were presented with profiles comprising three attributes (country of origin, price, and possibility of checking IUU fishing) and the levels of frozen poulp squid, the subject of the analysis. The participants were asked to select their preferred profile. The marginal willingness to pay for each attribute was derived from the results of the respondents' choices using conditional logit model estimates. Findings - There is a marked difference in utility based on the preference of the country of origin of fishery products among consumers. In addition, the utility of fishery products that have undergone IUU fishing verification was observed to be higher, with the utility marked to be higher for lower prices. Originality/value - Estimating the policy value of the risk management in IUU fishing of imported fisheries products in this study is a novel attempt that has never been conducted before. Several studies have been conducted to assess the risk of IUU fishing associated with the import of fishery products internationally. However, such studies are yet to be conducted in Korea. Instead, policies and studies have focused on issues related to complying with trading partners' legal and transparent standards for exporting fishery products. This study should be the beginning of more in-depth empirical and theoretical explorations to establish order in the domestic seafood market and respond to changes in international regulations on IUU fishing.

• The Journal of the Acoustical Society of Korea
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• v.42 no.6
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• pp.572-583
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• 2023

Demand for wood in construction is increasing worldwide. In Korea, technical reviews of high-rise Cross Laminated Timber (CLT) buildings are under way. In this paper, Floor Impact Sound Insulation Performance (FISIP) and Transmission Loss (TL) of 150 mm thick CLT floor panels made of two domestic species, Larix kaempferi and Pinus densiflora, are investigated. The CLT slabs were tested in reverberation chambers connected vertically. When comparing Single Number Quantity (SNQ) of FISIP of the bare panels, the Larix CLT is 3 dB lower in heavy-weight and 1 dB in light-weight than the Pinus CLT. However, there was no difference when concrete toppings were added to improve the performance. As the concrete toppings became thicker, the heavy-weight was reduced by 9 dB ~ 20 dB, and the light-weight by 20 dB ~ 30 dB. And the analysis of these results with area density has confirmed that the area densities are highly correlated (R² = 0.94 ~ 0.99) to the FISIP of the CLT. The types of CLT didn't affect the TL. Comparison of theoretical TL values with measured TL values has shown that the frequency characteristics are similar but 8 dB ~ 12 dB lower in measured values. The relationship between the TL and frequency characteristics of the tested CLT slabs was derived by using the correction value.

• Journal of Korean Society of Forest Science
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• v.112 no.4
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• pp.515-522
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• 2023

The aim of this study was to quantitatively evaluate a new stem volume table for estimating the growth, carbon storage, and greenhouse gas (GHG) absorption in Cryptomeria japonica and Chamaecyparis obtusa stands and to provide suggestions for improving the domestic GHG inventory. Carbon storage and GHG absorption were estimated using growing stock data obtained from invariable sub-sample plots between the 6^th and 7^th national forest inventories. We assessed changes in growing stock using the parameters employed by Kozak (1988) and Versions 1 and 2 of the stem volume table. Version 2 has new stem tables for 16 species, including Cryptomeria japonica, which were unavailable in Version 1. Version 2 also includes new data for trees with diameters at breast height equal to or greater than 30 cm. We found greater growing stock values using Version 2 than Version 1 for both stands, and the differences were statistically significant (p<0.001). Applying the new stem volume table increased GHG absorption by 22% for the Cryptomeria japonica stand and 13% for the Chamaecyparis obtusa stand. The growing stock estimation method used in this study should therefore be applied to re-estimate GHG absorptions in the forestry sector to produce accurate statistics for the IPCC guidelines.

• Journal of the Korean Institute of Landscape Architecture
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• v.52 no.2
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• pp.21-38
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• 2024

This study aims to establish an efficient street green area to improve the urban thermal environment and enhance pedestrian thermal comfort. Specifically, This study identified parameters applicable to green space planning and design, analyzed thermal environment mitigation mechanisms for each parameter, and, based on these findings, proposed methods for tree species selection and planting in green space planning and design. To achieve this, 61 papers were selected through a four-stage process from both domestic and foreign sources. The selected papers were analyzed, and the following main results were derived: In open street canyons with high stress levels due to low aspect ratios and high sky view factors(SVF), broadleaf trees with wide crown widths, low trunk heights, high leaf area index(LAI), and high crown heights were found effective in reducing heat, thereby increasing the amount and quality of shade. In contrast, in deep and narrow street canyons with relatively low heat stress due to high aspect ratios and low SVF, broad-leaved trees with narrow crown widths, high trunks, low crown heights, and low LAI were effective in reducing heat by enhancing ventilation. This study can serve as fundamental data for establishing standards for street green spaces to improve the thermal environment of street canyons and enhance thermal comfort of pedestrians. Additionally, it can be valuable when selecting the location and prioritizing street green spaces. Moreover, it is anticipated to be a foundational resource for creating guidelines for green space planning and design in response to climate change.

• Journal of the Korean Society of Marine Environment & Safety
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• v.12 no.4 s.27
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• pp.293-300
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• 2006

Domestic laws such as Korea Marine Pollution Prevention Law (KMPPL) which has been mae and amended according to the conclusions and amendments of various international conventions for the prevention a marine pollution such as MARPOL 73/78 were reviewed and compared with the major contents of the relevant international conventions. Alternative measures for legislating new laws or amending existing laws such as KMPPL for the acceptance of major contents of existing international conventions were proposed. Annex VI of MARPOL 73/78 into which the regulations for the prevention of air pollution from ship have been adopted has been recently accepted in KMPPL which should be applied to ships which are the moving sources of air pollution at sea rather tlnn in Korea Air Environment Conservation Law which should be applied to automobiles and industrial installations in land. The major contents of LC 72/95 have been accepted in KMPPL However, a few of substances requiring special care in Annex II of 72LC, a few of items in characteristics and composition for the matter in relation to criteria governing the issue of permits for the dumping of matter at sea in Annex III of 72LC, and a few of items in wastes or other matter that may be considered for dumping in Annex I of 96 Protocol have not been accepted in KMPPL yet. The major contents of OPRC 90 have been accepted in KMPPL. However, oil pollution emergency plans for sea ports and oil handling facilities, and national contingency plan for preparedness and response have not been accepted in KMPPL yet. The waste oil related articles if Basel Convention, which shall regulate and prohibit transboundary movement of hazardous waste, should be accepted in KMPPL in order to prevent the transfer if scrap-purpose tanker ships containing oil/water mixtures and chemicals remained on beard from advanced countries to developing and/or underdeveloped countries. International Convention for the Control if Harmful Anti-Fouling Systems on the Ships should be accepted in KMPPL rather tlnn in Korea Noxious Chemicals Management Law. International Convention for Ship's Ballast Water/Sediment Management should be accepted in KMPPL or by a new law in order to prevent domestic marine ecosystem and costal environment from the invasion of harmful exotic species through the discharge of ship's ballast water.