• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.43-49
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• 2018

The species of forage crops used in this study were Italian ryegrass (cv. Kowenery), sorghum (cv. SX17), proso millet (cv. domestic) and Japanese millet (cv. Jeju). The plant height of the summer crops was the highest at the dough stage. The dry matter yield of Italian ryegrass was 902.7 kg per 10 a. The dry matter yield of the winter crop and sorghum was 11,985 kg when harvested at the dough stage rather than at the first and second harvests. The proso and Japanese millet also had higher yields for dry matter during the dough stage rather than during heading and regeneration. The acid detergent fiber (ADF) content of Sorghum was lower than that of the first and second harvest; however, the proso and Japanese millet had a higher ADF content at the dough stage. The neutral detergent fiber (NDF) content was higher at the dough stage than at the first and second harvest, and the crude protein content was also lower at the dough stage than at the first and second harvest. The crude protein production for the dry matter yield was about 84 kg in Sorghum when harvested at the dough stage. Proso millet showed no difference for the crude protein production at the heading and dough stage while the Japanese millet had a higher crude protein production. There were no differences in the total digestible nutrients (TDN) content for the three crops according to the harvesting time. Therefore, if Sorghum and Proso and Japanese millet are to be combined with Italian ryegrass, it is better to harvest them at the dough stage.

• Environmental Analysis Health and Toxicology
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• v.31
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• pp.21.1-21.7
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• 2016

Objectives The present study analyzes metal contamination in sediment of the East Kolkata Wetlands, a Ramsar site, which is receiving a huge amount of domestic and industrial wastewater from surrounding areas. The subsequent uptake and accumulation of metals in different macrophytes are also examined in regard to their phytoremediation potential. Methods Metals like cadmium (Cd), copper (Cu), manganese (Mn), and lead (Pb) were estimated in sediment, water and different parts of the macrophytes Colocasia esculenta and Scirpus articulatus. Results The concentration of metals in sediment were, from highest to lowest, Mn ($205.0{\pm}65.5mg/kg$)>Cu ($29.9{\pm}10.2mg/kg$)>Pb ($22.7{\pm}10.3mg/kg$)>Cd ($3.7{\pm}2.2mg/kg$). The phytoaccumulation tendency of these metals showed similar trends in both native aquatic macrophyte species. The rate of accumulation of metals in roots was higher than in shoots. There were strong positive correlations (p <0.001) between soil organic carbon (OC) percentage and Mn (r =0.771), and sediment OC percentage and Pb (r=0.832). Cation exchange capacity (CEC) also showed a positive correlation (p <0.001) with Cu (r=0.721), Mn (r=0.713), and Pb (r=0.788), while correlations between sediment OC percentage and Cu (r=0.628), sediment OC percentage and Cd (r=0.559), and CEC and Cd (r=0.625) were significant at the p <0.05 level. Conclusions Bioaccumulation factor and translocation factors of these two plants revealed that S. articulatus was comparatively more efficient for phytoremediation, whereas phytostabilization potential was higher in C. esculenta.

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.2
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• pp.113-120
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• 2016

Bombyx mandarina is widely accepted as ancestor of B. mori. Silkworms are served as well-characterized models for understanding the mechanism for the genetic regulation of development. In this study, we performed RNA-Seq analysis to examine tissue-expression of gloverin isoforms of the silk-gland, mid-gut, and fat body in B. mandarina. BLAST analysis revealed that four gloverin isoform gene sequences of B. mandarina were highly similar to B. mori. To identify the difference between two species, the expression profile of gloverin was measured by semi- RT-PCR analysis. The specific expression of gloverin isoform genes was observed mainly in the fat body from B. mori but not B. mandarina. However, all of tissues in the wild-type silkworm could induce the upregulation of compared with the B. mori. To validate the sudden increase in gloverin gene expression in the mid-gut tissue of B. mandarina, we were using qRT-PCR. Relative mRNA expression rate of gloverin at the wild-type silkworm was much higher than domestic silkworm. Comparative genomics between domesticated and wild silkworms showed different tissue-expression levels in some of immune related genes. These results are suggesting a trend toward decreasing immunity related genes expression during domestication. Further studies are needed to elucidate the silkworm domestication and an invaluable resource for wild silkworm genomics research.

• Journal of Embryo Transfer
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• v.5 no.1
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• pp.1-20
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• 1990

Follicular atresia is a universal and characteristic phenomenon of both non-mammalian and mammalian vertebrates. Generally it is estimated that greater than 99% of follicles become atretic in higher domestic animals and human. The number of selected follicles developing to the preovulatory stage are thus fewer. Follicles can become atretic at any stage of development. The previous studies emphasized on descriptive and retrospect aspects of a limited population of the fully grown preovulatory follicle. The main efforts in ovarian physilogical researches are focused on follicular development culminating in ovulation but recent advances have resulted in a better understanding of atresia. Nowadays, recent studies are concentrated on the induction of atresia in a selected population of follicles and of the associated cellular, endocrine, biochemical and molecular changes. The factors initiating atresia and follicle selections are worthy of investigations. Another intriguing question is whether one can predict when a follicle will become atretic, i.e., what biochemical markers indicate that a follicle is destined for atresia. It is generally agreed that atretic process may vary even in antral follicles at different stages of their differentiations and among species. The dicisive factors are follicular responsiveness and the hormonal milieu. Some generalizations can be made on the basis of experimental induction of atresia. Alteration of the pattern of follicular steroid production is associated with the initiation stage of atretic process. Atresia appears to be a process unfolding gradually and affecting progressively in increasing number of functions and components of the follicle. The oocyte may be the latest to be afflicted in the atretic process. The high steroidogenic activity of atretic follicles lends support to the notion that atresia is not necessarily a degenerative process and that atretic follicles may play an essential role in ovarian physiology. The simultaneous occurence of growth and atretic processes may render the search for regulatory mechanisms involved in atresia difficult extremely. The questions such as how follicles are selected to undergo ovulation rather than atresia or what the mechanism of atresia is remain unanswered. However, the factors regulating or modifying ovarian hormonal milieu for the initiation of follicular growth and maturation or of atresia are being elucidated.

• Parasites, Hosts and Diseases
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• v.26 no.2
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• pp.121-126
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• 1988

Efficacy of praBiquantel (CesocideB injection) by intramuscular (1.M.) route against cestode infections was evaluated. Total 93 domestic or laboratory animals such as dogs, cats, rats, mice, goats, deers and chickens were used. Animals were infected with Dipylidium caninum, Spirometra sp. , Taenia pisiformis, Taenia taeniaeformis, Hymenolepis nana, Moniegia expanse, Moniexia sp. or Raillietina sp. A single dose of prasiquantel, 6 mg/kg of body weight, was highly effective (97.9%) against cestodes of various kinds disregarding the host species or their intensity of infection. At higher dose above 6 mg/kg, the cure rate was 100%. All the cestodes treated were expelled from the host within 48 hours. The discharged proglottides were damaged severely except Hymenolepis nana and Moniegia expanse. Intramuscular injection of this drug evoked a brief pain response in a dog, but no other side reactions were observed.

• Journal of the Korean Wood Science and Technology
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• v.42 no.1
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• pp.13-19
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• 2014

Three heat-treatment schedules were applied to $90{\times}90mm$ dimension square lumber of Pinus koraiensis, one of major domestic species, and their colors and physical properties were investigated for obtaining an optimum schedule. Each square lumber was heat-treated three times. The temperatures of $170^{\circ}C$ and $190^{\circ}C$, and the time of 9 hours and 13 hours were used for the first heat-treatment. The schedule of $190^{\circ}C$ and 7 hours were used for the next two heat-treatments. The averages of brightness $L^*$ decreased linearly as the heat-treatment repeated and its standard deviations also decreased slightly. While the averages of color difference ${\Delta}E^*$ increased linearly as the heat-treatment repeated and its standard deviations also increased slightly. The average compressive strength of the heat-treated specimen was higher than that of the control by 9%, which deviates from previous reports. ASE and WPG of the heat-treated specimens were measured to confirm that heat-treatment improved dimensional stability significantly.

• Journal of Environmental Science International
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• v.14 no.12
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• pp.1141-1153
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• 2005

Butyltin compounds (BTs), namely tributyltin (TBT), dibutyltin (DBT) and monobutyltin (MBT), were measured in surface and core sediments collected in 2001 inside Seogwipo Harbor, in order to evaluate their distribution characteristics. Seogwipo Harbor is one of major harbors of Jeju Island where a lot of shipping occurs and is designated as a trade port by the Korea Maritime and Port Law. BTs were detected in surface and core sediments of all stations and their concentrations in surface sediments were low, compared with to those in other sites of domestic and foreign countries. The main species among BTs was MBT, although there was a little difference with a survey site in surface sediments and in core sediments with depth. No or low correlations were obtained between organic matter or particles size of surface and core sediments and total BTs, indicating that these factors did not affect the distribution of BTs. It was estimated that more complex factors including BTs loads and surrounding sedimentary environments, affect the distribution of BTs. The high correlations between BTs indicated that DBT and MBT were mainly degraded from TBT based on antifouling paints of vessel etc. and other sources, such as DBT and MBT, could be ignored. The butyltin degradation indices ([DBT]+[MBT]/[TBT]) in surface sediments were in the range of $2.0\~3.8$ (mean 3.0), indicating that the parent compound, TBT, were inflowed into the surface sediments a long years ago, degraded and deposited. The sedimentation age of BTs contaminated core sediments could not estimated because the content of $^{210}pb$ activity were nearly all the same and so the sedimentation rate could not obtained.

• Journal of Environmental Science International
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• v.18 no.6
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• pp.691-698
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• 2009

Trickling filter has been extensively studied for the domestic wastewater treatment especially for the small scale plants in rural area. The performance of the trickling filter depends on the microbial community and their activity in the biofilms on the media. Nitrification. denitrification, and phosphorus removal of the trickling filter from the wastewater depend on the activity and the amount of the specific microorganisms responsible for the metabolism. For the estimation of the performance of a trickling filter, batch nitrification experiment and fluorescence in situ hybridization (FISH) were carried out to measure the microbial activity and its distribution on the media of the trickling filter. Batch nitrification activity measurement showed that the top part of the 1st stage trickling filter had the highest nitrification activity and the maximum activity was 0.002 g $NH_4$-N/g MLVSS${\cdot}$h. It is thought that higher substrate (ammonia) concentration yields more nitrifying bacteria in the biofilms. The dominant ammonia oxidizer and nitrite oxidizer in the biofilm were Nitrosomonas species and genus Nitrospira, respectively, by FISH analysis. Less denitrifiers were found than nitrifiers in the biofilm by the probe Rrp1088 which specifically binds to Rhodobacter, Rhodovulum, Roseobacter, and Paracoccus. Phosphorus accumulating bacteria were mostly found at the surface of the biofilm by probe Rc988 and PAO651 which specifically binds to Rhodocyclus group and their biomass was less than that of nitrifiers.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.2
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• pp.207-213
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• 2020

Cryopreservation is used for blastocyst preservation of most mammalian embryos and is an important technique for breeding. We aimed to compare the efficiency of the cryopreservation method using the standard Cryotop device and the ReproCarrier device, a domestic product manufactured in Korea. The efficacy of the two devices was analyzed based on the survival rate, intracellular levels of reactive oxygen species (ROS), and apoptosis of the vitrified bovine blastocysts. The survival rates of the vitrified-warmed blastocysts were similar between the ReproCarrier group (58.4 ± 17.7%) and Cryotop group (59.9 ± 14.1%). Intracellular ROS levels and apoptotic index were determined by DCFDA staining and TUNEL assay. Changes in intracellular ROS levels, number of total nuclei, and cellular apoptosis of vitrified blastocysts after cryopreservation were not significantly different between the two groups. These results indicate that the ReproCarrier device method is as effective as the standard Cryotop method for vitrification of bovine blastocysts in vitro.

• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.288-295
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• 2015

Salmonella enterica serovar Enteritidis is the predominant agent causing salmonellosis in chickens and other domestic animals. In an attempt to identify antigenic S. Enteritidis outer membrane proteins (OMPs) that may be useful for subunit vaccine development, we established a proteomic map and database of antigenic S. Enteritidis OMPs. In total, 351 and 301 spots respectively from S. Enteritidis strain 270 and strain 350 were detected by two-dimensional gel electrophoresis. Fifty-one antigen-reactive spots were detected by antisera on two-dimensional immunoblots and identified as 12 specific proteins by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. OmpA and DNA starvation/stationary phase protection protein (Dps) were the most abundant proteins among the identified OMPs, comprising 22 and 12 protein species, respectively. Interestingly, we found that the Dps of S. Enteritidis is also antigenic. OmpW was also verified to have high antigenicity. These results show that OmpA, Dps, and possibly OmpW are antigenic proteins. This study provides new insights into our understanding of the immunogenic characteristics of S. Enteritidis OMPs.