• Title/Summary/Keyword: diversity analysis

Search Result 4,360, Processing Time 0.036 seconds

Genetic Diversity and Clustering of the Rhoptry Associated Protein-1 of Plasmodium knowlesi from Peninsular Malaysia and Malaysian Borneo

  • Ummi Wahidah Azlan;Yee Ling Lau;Mun Yik Fong
    • Parasites, Hosts and Diseases
    • /
    • v.60 no.6
    • /
    • pp.393-400
    • /
    • 2022
  • Human infection with simian malaria Plasmodium knowlesi is a cause for concern in Southeast Asian countries, especially in Malaysia. A previous study on Peninsular Malaysia P. knowlesi rhoptry associated protein-1 (PkRAP1) gene has discovered the existence of dimorphism. In this study, genetic analysis of PkRAP1 in a larger number of P. knowlesi samples from Malaysian Borneo was conducted. The PkRAP1 of these P. knowlesi isolates was PCR-amplified and sequenced. The newly obtained PkRAP1 gene sequences (n=34) were combined with those from the previous study (n=26) and analysed for polymorphism and natural selection. Sequence analysis revealed a higher genetic diversity of PkRAP1 compared to the previous study. Exon II of the gene had higher diversity (π=0.0172) than exon I (π=0.0128). The diversity of the total coding region (π=0.0167) was much higher than those of RAP1 orthologues such as PfRAP-1 (π=0.0041) and PvRAP1 (π=0.00088). Z-test results indicated that the gene was under purifying selection. Phylogenetic tree and haplotype network showed distinct clustering of Peninsular Malaysia and Malaysian Borneo PkRAP1 haplotypes. This geographical-based clustering of PkRAP1 haplotypes provides further evidence of the dimorphism of the gene and possible existence of 2 distinct P. knowlesi lineages in Malaysia.

Genetic Diversity and Characterization of DPE1 Gene in Rice Germplasm

  • Aueangporn Somsri;Yong-Jin Park
    • Proceedings of the Korean Society of Crop Science Conference
    • /
    • 2022.10a
    • /
    • pp.220-220
    • /
    • 2022
  • Disproportionating Enzyme 1 (DPE1) is an a-1,4-D-glucanotransferase that cleavages the a-1,4-glucosidic bonds and transfers glucosyl groups. In rice endosperm, it participates in starch synthesis by transferring maltooligosyl groups from amylose and amylopectin to amylopectin. Here, we investigated the haplotype variations and evolutionary indices (e.g., genetic diversity and population structure) for the DPE1 gene in 374 rice accessions representing seven subgroups (wild, indica, temperate japonica, tropical japonica, aus, aromatic, and admixture). Variant calling analysis of DPE1 coding regions leads to the identification of six functional haplotypes representing/occupying 8 nonsynonymous SNPs. Nucleotide diversity analysis revealed the highest pi-value in wild group (0.0556) compared to other cultivated groups, of which temperate japonica showed the most reduction of genetic diversity value (0.003). A significant positive Tajima's D value (1.6330) of admixture highlights sudden population contraction under balancing selection, while temperate japonica with the lowest Tajima's D value (-1.3523) showed a selection signature of DPE1 domestication which might be the cause of excess of rare alleles. Moreover, these two subpopulations exhibits a greater differentiation (FST=0.0148), indicating a higher genetic diversity. Our findings on functional DPE1 haplotypes will be useful in future breeding programs, and the evolutionary indices can also be applicable in functional studies of the DPE1 gene.

  • PDF

Implications in UNESCO's Concept of 'Cultural Diversity' and Its Application to the 「World Geography」 Subject (유네스코 '문화 다양성' 개념의 함축과 「세계지리」 과목에서의 실천 방안)

  • Jeon, Jong-Han
    • Journal of the Korean Geographical Society
    • /
    • v.51 no.4
    • /
    • pp.559-576
    • /
    • 2016
  • The concept of 'cultural diversity' has emerged as a key concept and the buzzword of the 21st century's international community in education, science and culture sectors since UNESCO's "World Declaration on Cultural Diversity"(2001). However, the appropriate and correct implementation in educational level and in a subject scale can not be pursued without special understanding of multilateral implications of UNESCO's 'cultural diversity' because the concept of cultural diversity has been distributed to various applications according to individual scholars and institutions before the "World Declaration on Cultural Diversity" was released. The ultimate orientation of 'cultural diversity', the concept presented in "World Declaration on Cultural Diversity" is 'world peace' and 'the coexistence of various cultures of mankind'. In this regard, 'cultural diversity' has special 'educational' meaning to the next generation as well as the current one. Also, it is meaningful to take educational practices on cultural diversity in case of the "World Geography" subject in view of that international society of geographical education came up with "International Declaration of Geographical Education for Cultural Diversity" in the IGU(International Geographical Union) 2000. From this point of view, the author proposes that the 'cultural diversity' concept implies four folds of meanings as an ideology, as a symbol, as a vision, and as a epistemological turn based on the analysis of literatures on 'cultural diversity' of UNESCO, then presents an educational practice centering around the examples and its usage of teaching materials of cultural diversity.

  • PDF

Comparing Plant Species Diversity of Mountainous Deserts - Successes and Pitfalls

  • Van Etten, Eddie J.B.
    • The Korean Journal of Ecology
    • /
    • v.27 no.2
    • /
    • pp.79-86
    • /
    • 2004
  • An extensive study of the vegetation characteristics of the Hamersley Ranges, a mountainous desert area of north-west Australia, facilitated the comparison of plant species diversity measures with mountainous deserts of other parts of the world. Alpha diversity was defined as the number of species co-existing at local scales and was found to average 18 species per 0.1 ha for the Hamersley Ranges. This was found to be similar to seven other mountainous deserts in North and South America, and southern Africa. Variation in alpha diversity between these deserts was found to considerably lower than within deserts, suggesting that local processes control species richness at local scales. Beta diversity, defined here as turnover in species composition at various spatial scales, can be measured in many ways. For the Hamersley Ranges, Wilson's β ranged from 1.2 to 1.6 for five sites along a topographic gradient, whereas Whittaker's β between different plant communities was found to average 0.93. Comparable data was not found for other desert areas, but comparisons to non-desert areas suggest beta diversity within landscapes is relatively high and is likely to reflect the considerable landform heterogeneity of the Hamersley Ranges. 55∼70% of species were shared between different landscapes of the Hamersley Ranges; comparisons to other regions suggest beta diversity at this scale is relatively low. Gamma diversity, the number of species over large spatial extents, was successfully compared using regression analysis of the log-log species - area relationship. This revealed that the northern Sonoran desert has significantly less species than the Nama (inland) Karoo and Hamersley Ranges over medium spatial extents, but species numbers were similar at a regional scale. Several constraints to the valid comparison of species diversity were identified, including lack of standardisation of sampling techniques, the wide range of measures employed, general lack of published data, and the influence of the various components of spatial scale on most diversity measures. Recommendations on how to improve future comparative work are provided.

Genetic Diversity and Population Structure of a Korean Rice Germplasm Based on DNA Profiles

  • Lee, Kyung Jun;Lee, Jung-Ro;Shin, Myoung-Jae;Cho, Gyu-Taek;Ma, Kyung-Ho;Lee, Gi-An;Chung, Jong-Wook
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.63 no.1
    • /
    • pp.1-7
    • /
    • 2018
  • Information on the patterns of genetic diversity and population structure is essential for the rational use and efficient management of germplasms; accurate information aids in monitoring germplasms, and can also be used to predict potential genetic gains. In this study, we assessed genetic diversity, focusing on Korean rice accessions for theand their sustainable conserved diversity. Using DNA profiling with 12 simple sequence repeat (SSR) markers, we detected a total of 333 alleles among 2,016 accessions. The number of alleles ranged from 21 to 53, with an average of 27.8. Average polymorphism information content was 0.797, with the lowest being 0.667 and the highest 0.940. CA cluster analysis and the model-based population structure revealed two main groups that could be subdivided into five subgroups. Analysis of the molecular variance study based on the SSR profile data showed 5% variance among the profiles, whereas we recorded 93% variance among individuals and 2% variance within individuals. Specifically, the utilized diversity for of the breeding program is restricted in that cultivars were located in limited clades. These results revealed that preserving the diversity of Korean landraces could be useful sources for breeding new rice cultivars, and cwould be the basis for the sustainable conservation and utilization of a Korean rice germplasm.

An Analysis of Diversity and Specialization of Rural Amenity Resources - Case By Chungcheongnamdo Province - (농촌 어메니티자원의 다양성과 특화도 분석 - 충청남도 시군단위를 대상으로 -)

  • Chang, Woo Seok;Jung, Nam Su
    • Journal of Korean Society of Rural Planning
    • /
    • v.20 no.3
    • /
    • pp.33-43
    • /
    • 2014
  • The Purpose of this study is to analyze of Specialization and Diversity of Rural Amenity Resources Planning. It will be performed analysis of the degree of specialization and diversity in each city and district of Chungcheongnam-do Province. Specialization and diversity indices were calculated by separately category of rural amenity resources into natural, cultural, and societal resources. The results of calculating the specialization degree of rural amenity resources based on a location index showed that eleven regions (Asan City, Buyeo District, Gyeryong City, Gongju City, Nonsan City, Boryeong City, Seocheon District, Seosan City, Seajong District, Cheongyang District and Yesan City) specialized in natural resources; four regions (Geumsan District and Hongseong Distriect) specialized in cultural resources; and six regions (Dangjin District, Cheonan City) specialized in societal resources. Examining resource specialization degrees by regions, there was a high number of regions specialized in natural and societal resources while those specialized in cultural resources were the rarest. Examining the calculation results for the tendency of resource specialization degree by areas showed that, with natural and cultural resources specialized regions, the average specialization degree showed a clear distinction with a 2.19 natural resource specialization degree and a 2.05 societal resource degree; However, in contrast, areas specialized in cultural resources-with an average degree of 1.52-were judged as not being especially more specialized than the average degree of Chungcheongnam-do Province. Also, there was seen a great gap between regions with high diversity and regions with low diversity of rural amenities, and measures to solve this difference is required.

Seasonal and Spatial Diversity of Picocyanobacteria Community in the Great Mazurian Lakes Derived from DGGE Analyses of 16S rDNA and cpcBA-IGS Markers

  • Jasser, Iwona;Krolicka, Adriana;Jakubiec, Katarzyna;Chrost, Ryszard J.
    • Journal of Microbiology and Biotechnology
    • /
    • v.23 no.6
    • /
    • pp.739-749
    • /
    • 2013
  • The seasonal and spatial diversity of picocyanobacteria (Pcy) in lakes of the Great Mazurian Lakes (GLM) system was examined by DGGE analysis of molecular markers derived from the 16S-23S internal transcribed spacer (ITS) of the ribosomal operon and the phycocyanin operon (cpcBA-IGS). The study of nine lakes, ranging from mesotrophy to hypereutrophy, demonstrated seasonal variance of Pcy. The richness and Shannon diversity index calculated on the basis of both markers were higher in spring and lower in early and late summer. No statistically significant relationships were found between the markers and trophic status of the studied lakes or Pcy abundance. There were, however, statistically significant relationships between the diversity indices and sampling time. The analysis pointed to a different distribution of the two markers. The ITS marker exhibited more unique sequences in time and space, whereas a greater role for common and ubiquitous sequences was indicated by the cpcBA-IGS data. Examination of the Pcy community structure demonstrated that communities were grouped in highly similar clusters according to sampling season/time rather than to the trophic status of the lake. Our results suggest that time is more important than trophic status in shaping the diversity and structure of Pcy communities. The seasonal changes in picocyanobacteria and differences in diversity and community structures are discussed in the context of well-established ecological hypotheses: the PEG model, intermediate disturbance hypothesis (IDH), and horizontal gene transfer (HGT).

Phylogeographic patterns in cryptic Bostrychia tenella species (Rhodomelaceae, Rhodophyta) across the Thai-Malay Peninsula

  • Bulan, Jakaphan;Maneekat, Sinchai;Zuccarello, Giuseppe C.;Muangmai, Narongrit
    • ALGAE
    • /
    • v.37 no.2
    • /
    • pp.123-133
    • /
    • 2022
  • Genetic diversity and distribution patterns of marine macroalgae are increasingly being documented in Southeast Asia. These studies show that there can be significant levels of genetic diversity and isolation between populations on either side of the Thai-Malay Peninsula. Bostrychia tenellla is a common filamentous red seaweed in the region and the entity is represented by at least two cryptic species. Despite being highly diverse and widespread, genetic variation and population structure of this species complex remains understudied, especially around the Thai-Malay Peninsula. We analyzed genetic diversity and inferred the phylogeographic pattern of specimens identified as B. tenella using the plastid RuBisCo spacer from samples from the Andaman Sea and the Gulf of Thailand. Our genetic analysis confirmed the occurrence of the two cryptic B. tenella species (B and C) along both coasts. Cryptic species B was more common in the area and displayed higher genetic diversity than species C. Historical demographic analyses indicated a stable population for species B, but more recent population expansion for species C. Our analyses also revealed that both cryptic species from the Andaman Sea possessed higher genetic diversity than those of the Gulf of Thailand. We also detected moderate to high levels of gene flow and weak phylogeographic structure of cryptic species B between the two coasts. In contrast, phylogeographic analysis showed genetic differences between populations of both cryptic species within the Andaman Sea. Overall, these results suggest that cryptic B. tenella species around Thai-Malay Peninsula may have undergone different demography histories, and their patterns of genetic diversity and phylogeography were likely caused by geological history and regional sea surface current circulation in the area.

Performance Evaluation and Theoretical Model for the Polarization Diversity using Circularly Polarized Waves in N-LOS Radio Environments (비가시거리 전파환경에서 원형편파를 이용한 편파다이버시티의 이론적 모델 및 성능평가)

  • 이주현;하덕호;박정훈
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.14 no.2
    • /
    • pp.133-138
    • /
    • 2003
  • In this paper, we analyzed a two-branch polarization diversity at a mobile station in NLOS environment when a base station transmits a circularly polarized wave. In order to calculate the correlation coefficient considering the XPD(cross polarization discrimination) between the received signals for the two diversity branches, a simple theoretical model of circular polarization diversity is adopted. From the analysis results, it can be seen that the XPD of circularly polarized wave is less than vertically polarized wave about 6~7 dB in measurement results. And also, it is clearly seen that the correlation coefficient of circular polarization diversity evaluated by the XPD is less than that of vertical polarization diversity.

The Classification and Characteristics of Landscape on Urban Land Use Patterns - The Case of Metropolitan Daejeon - (도시의 토지이용 형태별 경관특성과 유형 - 대전광역시를 사례로 -)

  • Kim Dae-Hyun;Kim Dae-Soo;Joo Shin-Ha;Oh Se-Rae
    • Journal of the Korean Institute of Landscape Architecture
    • /
    • v.33 no.4 s.111
    • /
    • pp.1-10
    • /
    • 2005
  • Recently, as urban landscape is growing in importance, urban landscape planning is being actively performed. for this purpose, classification of the urban landscape is definitely required. Therefore, this research focuses on classifying urban landscape in Daejeon metropolis by dividing the urban land use pattern. This results are as follows. 1. Urban land use pattern is divided into 20 classes. The residential, commercial and industrial areas, the old market and the bus terminal are evaluated negatively, whereas the areas of school, water reservoir, neighborhood park and train station are appreciated as being positive in landscape characters. 2. As a result of a cluster analysis, urban landscape has five different landscape types. These are: landscapes of medium diversity lacking green area, landscapes of high diversity lacking green area, landscapes rich in green area and with medium diversity, landscapes rich in green area and with high diversity, and landscapes rich in green area and with low diversity. 3. In landscape characters of beauty and harmony, landscapes rich in green area and with medium diversity are more positively evaluated than those rich in green area and with low diversity. This point should be taken into account for planning the urban landscape.