• Title/Summary/Keyword: diversity analysis

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Morphological Characteristics and Genetic Diversity Analysis of Platycodon grandiflorum (Jacq.) A. DC Determined Using SSR Markers (도라지 수집종의 형태적 특성과 SSR마커에 의한 유연관계 분석)

  • Um, Yurry;Lee, Yi;Jin, Mei-Lan;Lee, Dae Young;Lee, Jae Won;Kim, Geum Soog;Kim, Chang Kug;Hong, Chang Pyo;Kim, Ok Tae
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.1
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    • pp.55-61
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    • 2016
  • Background : Plant breeding requires the collection of genetically diverse genetic resources. Studies on the characteristics of Platycodon grandiflorum resources have not been carried out so far. The present study was carried out to discriminate P. grandiflorum based on morphological characteristics and genetic diversity using simple sequence repeat (SSR) markers. Methods and Results :We collected 11 P. grandiflorum cultivars: Maries II, Hakone double white, Hakone double blue, Fuji white, Fuji pink, Fuji blue, Astra white, Astra pink, Astra blue, Astra semi-double blue and Jangbaek. Analyses of the morphological characteristics of the collection were conducted for aerial parts (flower, stem and leaf) and underground parts (root). Next, the genetic diversity of all P. grandiflorum resources was analyzed using SSR markers employing the DNA fragment analysis method. We determined that the 11 P. grandiflorum cultivars analyzed could be classified by plant length, leaf number and root characteristic. Based on the genetic diversity analysis, these cultivars were classified into four distinct groups. Conclusions : These findings could be used for further research on cultivar development using molecular breeding techniques and for conservation of the genetic diversity of P. grandiflorum. Moreover, the markers could be used for genetic mapping of the plant and marker-assisted selection for crop breeding.

Genetic diversity analysis of Thai indigenous pig population using microsatellite markers

  • Charoensook, Rangsun;Gatphayak, Kesinee;Brenig, Bertram;Knorr, Christoph
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.10
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    • pp.1491-1500
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    • 2019
  • Objective: European pigs have been imported to improve the economically important traits of Thai pigs by crossbreeding and was finally completely replaced. Currently Thai indigenous pigs are particularly kept in a small population. Therefore, indigenous pigs risk losing their genetic diversity and identity. Thus, this study was conducted to perform large-scale genetic diversity and phylogenetic analyses on the many pig breeds available in Thailand. Methods: Genetic diversity and phylogenetics analyses of 222 pigs belonging to Thai native pigs (TNP), Thai wild boars (TWB), European commercial pigs, commercial crossbred pigs, and Chinese indigenous pigs were investigated by genotyping using 26 microsatellite markers. Results: The results showed that Thai pig populations had a high genetic diversity with mean total and effective ($N_e$) number of alleles of 14.59 and 3.71, respectively, and expected heterozygosity ($H_e$) across loci (0.710). The polymorphic information content per locus ranged between 0.651 and 0.914 leading to an average value above all loci of 0.789, and private alleles were found in six populations. The higher $H_e$ compared to observed heterozygosity ($H_o$) in TNP, TWB, and the commercial pigs indicated some inbreeding within a population. The Nei's genetic distance, mean $F_{ST}$ estimates, neighbour-joining tree of populations and individual, as well as multidimensional analysis indicated close genetic relationship between Thai indigenous pigs and some Chinese pigs, and they are distinctly different from European pigs. Conclusion: Our study reveals a close genetic relationship between TNP and Chinese pigs. The genetic introgression from European breeds is found in some TNP populations, and signs of genetic erosion are shown. Private alleles found in this study should be taken into consideration for the breeding program. The genetic information from this study will be a benefit for both conservation and utilization of Thai pig genetic resources.

Application of genomic big data to analyze the genetic diversity and population structure of Korean domestic chickens

  • Eunjin Cho;Minjun Kim;Jae-Hwan Kim;Hee-Jong Roh;Seung Chang Kim;Dae-Hyeok Jin;Dae Cheol Kim;Jun Heon Lee
    • Journal of Animal Science and Technology
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    • v.65 no.5
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    • pp.912-921
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    • 2023
  • Genetic diversity analysis is crucial for maintaining and managing genetic resources. Several studies have examined the genetic diversity of Korean domestic chicken (KDC) populations using microsatellite markers, but it is difficult to capture the characteristics of the whole genome in this manner. Hence, this study analyzed the genetic diversity of several KDC populations using high-density single nucleotide polymorphism (SNP) genotype data. We examined 935 birds from 21 KDC populations, including indigenous and adapted Korean native chicken (KNC), Hyunin and Jeju KDC, and Hanhyup commercial KDC populations. A total of 212,420 SNPs of 21 KDC populations were used for calculating genetic distances and fixation index, and for ADMIXTURE analysis. As a result of the analysis, the indigenous KNC groups were genetically closer and more fixed than the other groups. Furthermore, Hyunin and Jeju KDC were similar to the indigenous KNC. In comparison, adapted KNC and Hanhyup KDC populations derived from the same original species were genetically close to each other, but had different genetic structures from the others. In conclusion, this study suggests that continuous evaluation and management are required to prevent a loss of genetic diversity in each group. Basic genetic information is provided that can be used to improve breeds quickly by utilizing the various characteristics of native chickens.

An Analysis of Nationality Diversity by Journal Editors and Authors Using Diversity Index: Focusing on International Journals of Library and Information Science (다양성 지수를 활용한 학술지 편집위원과 저자의 국적 다양성 분석 - 문헌정보학 분야 국제학술지를 중심으로 -)

  • Jane Cho
    • Journal of the Korean Society for Library and Information Science
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    • v.58 no.2
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    • pp.81-100
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    • 2024
  • This study analyzed the national diversity of journal editors and authors, focusing on international journals in library and information science, and quantified it through Simpson's Diversity Index. In addition, LIS journals were clustered according to diversity indicators and their characteristics were analyzed, and it was also confirmed whether nationality diversity of editorial members could induce nationality diversity of authors. As a result of the analysis, first, the diversity index of the editors of the LIS (Library & Information Science) international journal was found to be 0.64, which is less than the diversity of the authors, which is 0.76. It was confirmed that as the diversity of nationalities among editorial committee members increases, it is possible to attract authors of various nationalities (r = .79). Second, journals in the IS (Information Science) field showed a high index before or after 0.8 for both editorial members and author contributing countries, but those in the LS (Library Science) field showed relatively low scores, especially among the editorial members, with a very low index of 0.48. Third, the United States has the highest share of both authors and editors, and in particular, it has been confirmed that the LS field has an overwhelming share of 53.39% of editors. On the other hand, in Asian countries, including Korea, the ratio of contributions as editors compared to contributions as authors was found to be lower than in the United States and European countries.

Genetic Diversity of Ascaris in China Assessed Using Simple Sequence Repeat Markers

  • Zhou, Chunhua;Jian, Shaoqing;Peng, Weidong;Li, Min
    • Parasites, Hosts and Diseases
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    • v.56 no.2
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    • pp.175-181
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    • 2018
  • The giant roundworm Ascaris infects pigs and people worldwide and causes serious diseases. The taxonomic relationship between Ascaris suum and Ascaris lumbricoides is still unclear. The purpose of the present study was to investigate the genetic diversity and population genetic structure of 258 Ascaris specimens from humans and pigs from 6 sympatric regions in Ascaris-endemic regions of China using existing simple sequence repeat data. The microsatellite markers showed a high level of allelic richness and genetic diversity in the samples. Each of the populations demonstrated excess homozygosity (Ho0). According to a genetic differentiation index (Fst=0.0593), there was a high-level of gene flow in the Ascaris populations. A hierarchical analysis on molecular variance revealed remarkably high levels of variation within the populations. Moreover, a population structure analysis indicated that Ascaris populations fell into 3 main genetic clusters, interpreted as A. suum, A. lumbricoides, and a hybrid of the species. We speculated that humans can be infected with A. lumbricoides, A. suum, and the hybrid, but pigs were mainly infected with A. suum. This study provided new information on the genetic diversity and population structure of Ascaris from human and pigs in China, which can be used for designing Ascaris control strategies. It can also be beneficial to understand the introgression of host affiliation.

The Ecological Characteristics of Classified Forest Cover Types in the Natural Forest of Sobaeksan

  • Lim, Seon-Mi;Kim, Ji Hong
    • Journal of Forest and Environmental Science
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    • v.31 no.2
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    • pp.126-135
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    • 2015
  • This study was conducted to evaluate the ecological characteristics of forest cover types which were classified by cluster analysis in the natural forest of Sobaeksan on the basis of the vegetation data from the point-quarter sampling method. Recognized forest cover types were 1) Mixed mesophytic forest, 2) Taxus cuspidata forest, 3) Fraxinus rhynchophylla-Quercus mongolica forest, 4) Betula ermanii forest, 5) Pinus densiflora forest, 6) Quercus mongolica mixed forest, and 7) Quercus mongolica pure forest. For those of classified types, the species composition was expressed by importance value (IV) to describe the community floristically. The species diversity was quantified using the Shannon's diversity index. The results showed that the forest cover types were characteristically different from one another in growing species and compositional rates, depending upon the type which was formed by a number of similar vegetational sample points. Species diversity indices (H') of total and overstory both were the highest in the mixed mesophytic forest (3.530 and 2.880, respectively), and lowest in the Q. mongolica pure forest (2.122 and 0.000, respectively) with only one canopy species. The highest species diversity in the mixed mesophytic forest may due to the relatively high species richness and evenness in the forest cover types. The description on ecological characteristics were suggested to understand the formation and development of forest cover types in this study area.

A Study on the Plant Succession Structural Analysis in Expressway Slope I (고속도로 비탈면의 식생천이 특성 연구 I)

  • Jeon, Gi-Seong
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.16 no.4
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    • pp.41-52
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    • 2013
  • This study was carried out in order to analyze the succession characteristics of the slope of expressways and the results of analysis by collecting and analyzing various literatures and data from 2011 to 2012 and surveying 75 expressway slope, as follows : The construction methods applied to planting the slope of an expressway can be classified into 4 types including 3 Climber planting methods, 8 Vegetation thick-layer spray work methods, 4 Seed spray work methods, and 4 Stabilization work methods. The factors which affect the cover degree of the slope of an expressway were found to be development years, gradient, length, and azimuth. Like surrounding forest areas, the expressway slope was analyzed to begin the plant succession 20, 30, and 40 years after development, and plant succession was developed in diversity in a mixed stand forest according to surrounding forest floors. Species diversity, maximum species diversity, and the evenness of slope facing north were analyzed to be high comparing to those facing east, west, and south according to azimuth of slope. Species diversity, maximum species diversity, evenness of slope, and the plant succession of surroundings were analyzed to be high when the gradient of the slope was less than $40^{\circ}$. The dominant species which success on the slope of an expressway and its surrounding forest area included Miscanthus sinensis var. purpurascens, Zoysia japonica, Pinus thunbergii Parl, Rubus crataegifolius, Lespedeza cyrtobotrya, Amorpha fruticosa, Artemisia princeps var. orientalis, and Oenothera biennis.

Assessment of genetic diversity and distance of three Cicuta virosa populations in South Korea

  • Nam, Bo Eun;Kim, Jae Geun;Shin, Cha Jeong
    • Journal of Ecology and Environment
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    • v.36 no.3
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    • pp.205-210
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    • 2013
  • Cicuta virosa L. (Apiaceae) is a perennial emergent plant designated as an endangered species in South Korea. According to the former records, only four natural habitats remain in South Korea. A former study suggested that three of four populations (Pyeongchang: PC, Hoengseong: HS, Gunsan: GS) would be classified as different ecotypes based on their different morphological characteristics and life cycle under different environmental conditions. To evaluate this suggestion, we estimated genetic diversity in each population and distance among three populations by random amplification of polymorphic DNA. Seven random primers generated a total of 61 different banding positions, 36 (59%) of them were polymorphic. Nei's gene diversity and the Shannon diversity index increased in the order of PC < HS < GS, which is the same order of population size. In the two-dimensional (2D) plot of first two principal components in principal component analysis with the presence of 61 loci, individuals could be grouped as three populations easily (proportion of variance = 0.6125). Nei's genetic distance for the three populations showed the same tendency with the geographical distance within three populations. And it is also similar to the result of discriminant analysis with the morphological or life-cycle factors from the previous study. From the results, we concluded that three different populations of C. virosa should be classified as ecotypes based on not only morphology and phenology but genetic differences in terms of diversity and distance as well.

Genetic Diversity of the Pear Scab Fungus Venturia nashicola in Korea

  • Choi, Eu Ddeum;Kim, Gyoung Hee;Park, Sook-Young;Song, Jang Hoon;Lee, Young Sun;Jung, Jae Sung;Koh, Young Jin
    • Mycobiology
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    • v.47 no.1
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    • pp.76-86
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    • 2019
  • Scab disease caused by Venturia nashicola is of agroeconomic importance in cultivation of Asian pear. However, little is known about the degree of genetic diversity in the populations of this pathogen. In this study, we collected 55 isolates from pear scab lesions in 13 major cultivation areas in Korea and examined the diversity using sequences of internal transcribed spacer (ITS) region, ${\beta}$-tubulin (TUB2), and translation elongation factor-$1{\alpha}$ ($TEF-1{\alpha}$) genes as molecular markers. Despite a low level of overall sequence variation, we found three distinctive subgroups from phylogenetic analysis of combined ITS, TUB2, and $TEF-1{\alpha}$ sequences. Among the three subgroups, subgroup 1 (60% of isolates collected) was predominant compared to subgroup 2 (23.6%) or subgroup 3 (16.4%) and was distributed throughout Korea. To understand the genetic diversity among the subgroups, RAPD analysis was performed. The isolates yielded highly diverse amplicon patterns and none of the defined subgroups within the dendrogram were supported by bootstrap values greater than 30%. Moreover, there is no significant correlation between the geographical distribution and the subgroups defined by molecular phylogeny. Our data suggest a low level of genetic diversification among the populations of V. nashicola in Korea.

The Effect of Openness to Diversity on Innovative Behavior: The Mediating Effect of Unit-members Trust (장병의 다양성 수용도가 혁신행동에 미치는 영향: 부대원 신뢰의 매개효과)

  • Jeon, Je-Man;Park, Chun-Seok;Moon, Sung-Ok
    • Asia-Pacific Journal of Business
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    • v.12 no.4
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    • pp.211-225
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    • 2021
  • Purpose - This study deals with the Openness to Diversity(OD) of soldiers in the military organization for efficient unit operation of the military organization composed of various soldier. The purpose of this study was to examine the effects of information and value OD on innovative behavior and the mediating effect of unit-members trust. Design/methodology/approach - The 269 samples of this study were surveyed on army soldiers. The Exploratory Factor Analysis (EFA), the multiple regression, bootstraping analysis were hired in order to analyze the data. Findings - The results showed that the information & value OD were positive(+) effect on innovative behavior. Unit-members trust showed a mediation effect between OD and innovative behavior. Research implications or Originality - First, according to Defense Reform 2.0, the military is expected to be composed of more diverse classes in the future. There is a theoretical contribution that examine their perception of diversity within the military. Second, OD was confirmed as a variable predicting the innovative behavior of soldiers and unit-memeber trust mediate the relationship between OD and invative behavior.