• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.161-167
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• 2007

In this study, we constructed and tested retrovirus vectors designed to express the human thrombopoietin gene under the control of the tetracycline-inducible promoters. To increase the hTPO gene expression at him-on state, WPRE sequence was also introduced into retrovirus vector at downstream region of either the hTPO gene or the sequence encoding reverse tetracycline-controlled transactivator (rtTA). Primary culture cells (PFF, porcine fetal fibroblast; CEF, chicken embryonic fibroblast) infected with the recombinant retrovirus were cultured in the medium supplemented with or without doxycycline for 48hr, and induction efficiency was measured by comparing the hTPO gene expression level using RT-PCR, western blot and ELISA. Higher hPTO expression and tighter expression control were observed from the vector in which the WPRE sequence was placed at downstream of the hTPO (in CEF) or rtTA(in PFF) gene. This resulting tetracycline inducible vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.6
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• pp.122-130
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• 2013

The demand and the supply are increasing sharply in accordance with the growth of the Memory Semiconductor Industry. The Flash Memory above all is being utilized substantially in the Industry of smart phone, the tablet PC and the System on Chip (SoC). The Flash Memory is divided into the NOR-type Flash Memory and the NAND-type Flash Memory. A lot of study such as the Built-In Self Test (BIST), the Built-In Self Repair (BISR) and the Built-In Redundancy Analysis (BIRA), etc. has been progressed in the NOR-type fash Memory, the study for the Built-In Self Test of the NAND-type Flash Memory has not been progressed. At present, the pattern test of the NAND-type Flash Memory is being carried out using the outside test equipment of high price. The NAND-type Flash Memory is being depended on the outside equipment as there is no Built-In Self Test since the erasure of block unit, the reading and writing of page unit are possible in the NAND-type Flash Memory. The Built-In Self Test equipped with 2 kinds of finite state machine based structure is proposed, so as to carry out the pattern test without the outside pattern test equipment from the NAND-type Flash Memory which carried out the test dependant on the outside pattern test equipment of high price.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.10
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• pp.3873-3879
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• 2010

Home service robot are not working in the fixed task such as industrial robot, because they are together with human in the same indoor space, but have to do in much more flexible and various environments. Most of them are developed on the base of the wheel-base mobile robot in the same method as a vehicle robot for factory automation. In these days, for holonomic system characteristics, omni-directional wheels are used in the mobile robot. A holonomicrobot, using omni-directional wheels, is capable of driving in any direction. But trajectory control for omni-directional mobile robot is not easy. Especially, azimuth control which sensor uncertainty problem is included is much more difficult. This paper develops trajectory controller of 3-wheels omni-directional mobile robot using fuzzy azimuth estimator. A trajectory controller for an omni-directional mobile robot, which each motor is controlled by an individual PID law to follow the speed command from inverse kinematics, needs a precise sensing data of its azimuth and exact estimation of reference azimuth value. It has imprecision and uncertainty inherent to perception sensors for azimuth. In this paper, they are solved by using fuzzy logic inference which can be used straightforward to perform the control of the mobile robot by means of the fuzzy behavior-based scheme already existent in literature. Finally, the good performance of the developed mobile robot is confirmed through live tests of path control task.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.3
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• pp.104-111
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• 2018

The sensorless control of high efficiency air compressors using a permanent magnet type synchronous motor as an oil-free air compressor is quite common. However, due to the nature of the air compressor, it is difficult to install a position sensor. In order to control the permanent magnet type synchronous motor at variable speed, the inclusion of a position sensor to grasp the position of the rotor is essential. Therefore, in order to achieve sensorless control, it is essential to use a permanent magnet type synchronous motor in the compressor. The position estimation method based on the back electromotive force, which is widely used as the sensorless control method, has a limitation in that position errors occur due either to the phase delay caused by the use of a stationary coordinate system or to the estimated back electromotive force in the transient state caused by the use of a synchronous coordinate system. Therefore, in this paper, we propose a method of estimating the position and velocity using a rotation angle tracking observer and reducing the speed ripple through a disturbance observer. An experimental apparatus was constructed using Freescale's MPU and the feasibility of the proposed algorithm was examined. It was confirmed that even if a position error occurs at a certain point in time, the position correction value converges to the actual vector position when the position error value is found.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.6
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• pp.109-126
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• 2015

This study is for constructing detailed data to secure biological diversity and maintain a healthy ecosystem in Backsasil Valley Ecological and Scenery Conservation Area, and for preparing an ecological management plan fit for the protected area. The period for this study was from April 2010 to May 2013. Recently, the increase of visitors to Baeksasil Valley, as well as the plantation and dissemination of introduced vegetable species, become factors accelerating ecological disturbance. Major research contents included the reviews of the management system of the protected areas, the necessity of ecological management at the basin level, establishing the system of a management plan, research and analysis of environment ecology, analysis of threatening factors, goal-setting, management strategies, and a plan through SWOT analysis. Survey items were the natural environment, major components of ecosystem, and human-use. The goals of ecological management were basin zone management for amphibians, conservation and restoration of forest vegetation for conservation habitat of Dryocopus martius, conservation habitat of Zelkova serrta for nature landscape, and management of users for environment protection. The conservation management plan at a basin zone level contains the management of point and non-point pollution sources in the upstream, securing growing conditions for native plants, securing safe habitats for amphibians, and securing of habitats for major wild birds. Also, restoration of natural forests, management of native plants and introduced vegetable species, and restoration of degraded forest paths are suggested for the restoration plans.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.9
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• pp.666-674
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• 2013

Physical disturbance, which induces a lack of flow rate, frequently occurs in freshwater ecosystem. Due to this, it is required to provide a new fish shelter to resolve. We installed a pilot scale test-bed to scrutinize the relationship between water level and the influence of fish shelter. The proposed ADP (artificial deep pool) is a fish shelter which composed of concrete materials. From the monitoring results in test-bed, it was observed that the population of fish was the highest at the 0.5 m in depth from the water level of experimental pond. But it was more appropriate for shallow water level (<0.3 m) to conserve the total number of fish by increasing the number per unit area despite of lower inner temperature and DO than outer environment. Therefore, inner of ADP was more efficient lentic system for fish to live due to higher WCS, OS, SS, and TS. In addition, there was a relative abundance of WCS fish species such as Acheilognathus koreensis (A. koreensis), Carassius carassius (C. carassius). Considered these results, it is suggested that ADP is appropriate to use for fish shelter and habitat for the fishes in lentic ecosystem.

• Journal of Biomedical Engineering Research
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• v.24 no.5
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• pp.381-389
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• 2003

Various adult diseases (cerebral apoplexy, athymiait, etc.) result from hypertension, blood circulation disturbance and increment of HBP. In early diagnosis of these diseases, MRI, X-ray and PET have been used rather aim for treatment than for a prevention of disease. Since. cerebral apoplexy and athymiait could appear to the regular/irregular persons, it is very important to measure HBP which has connection with cerebral blood flow state. HBP has more diagnosis elements than that of BP. So, we can diagnose accurate hypertension by measuring of HBP. But, existing sphygmomanometers and automatic BP monitors can not measure HBP, and can not execute complex function(measuring of BP/HBP, blood flow improvement). Purpose of this paper is to develop a system and algorithm which can measure BP/HBP for accurate diagnosis. Also, we extracted diagnosis factors by correlativity analysis of BP/HBP. Maximum pressure of HBP corresponds to 62% that of BP, Minimum pressure of HBP corresponds to 46% that of BP. Therefore, we developed the multi-function automatic blood pressure monitor which can measure BP/HBP and improve cerebral blood flow state.

• Korean Journal of Ecology and Environment
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• v.39 no.4 s.118
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• pp.424-434
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• 2006

This study investigates bacteria-zooplankton grazing link and factors affecting their grazing relationship at trophically different two sites (Paldang Dam and Kyungan Stream) of Lake Paldang Ecosystem from April to December, 2005. Zooplankton were divided into two size groups; microzooplankton (MICZ) : 60-200 ${\mu}m$ and macrozooplankton (MACZ): >200 ${\mu}m$), and their grazing rates on bacteria were conducted for each size group separately. Bacterial abundance and seasonal change pattern were similar between two sites. MICZ, mostly rotifers (e.g., Brachionus, Keratella, Polyathra) were numerically dominant at both sites, while carbon biomass was highest in cladocerans. Zooplankton biomass was higher at the Kyungan Steam site compared to Paldang Dam site, and their high biomass during spring decreased as they were passing through the storm events in summer season at both sites. Zooplankton clearance rate (CR) was high in spring and autumn while low in summer at Paldang Dam site. However, zooplankton CR was high during the summer at Kyungan Stream site. Bacterial C-flux was high in spring and autumn when MACZ (esp. cladecerans) developed at a high biomass level at both sites. Overall, MACZ community CR and carbon flux (C-flux) were higher than those of MICZ, and the degree of difference between them was higher at Kyungan Stream site. Short hydraulic residence time and physical disturbance caused by summer storm event appeared to affect the zooplankton grazing on bacteria at both sites. The results of this study indicate that bacteria are potentially important carbon source of zooplankton, and that both biotic (e.g,, prey and predator taxa composition and abundance) and physical parameters appear to alter energy transfer in the planktonic food web of this river-reservoir hybrid system.

• Korean Journal of Environment and Ecology
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• v.34 no.2
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• pp.142-156
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• 2020

This study surveyed Gyeongan Stream, a tributary of the Han River Drainage System, from April to October 2017 to investigate the characteristics of fish communities. The survey collected 40 species of 11 families from 48 survey stations using kick nets and cast nets. The dominant and subdominant species were Zacco platypus (48.0%) and Rhynchocypris oxycephalus (17.7%), respectively. The next most abundant species were Pungtungia herzi (6.8%), Z. koreanus (5.0%), Carassius auratus (4.3%), Squalidus gracilis majimae (2.7%), and Rhodeus notatus (2.5%). Among the fish collected, 14 species (35.0%) were Korean endemic species, and the exotic species were Micropterus salmoides, Lepomis macrochirus, and Cyprinus carpio (Israeli type). The land-locked species were Plecoglossus altivelis, Rhinogobius brunneus, and Cottus koreanus, while the species sensitive to climate change was C. koreanus. The community analysis showed that the dominance was higher at the uppermost stream station, whereas diversity and abundance tended to be lower at the upstream station and higher toward the downstream station. The community structure was largely divided into rivers (uppermost stream, upstream, and middle-lower stream) and lake. The river health was mostly good (23 stations, 47.9%) and fair (15 stations, 31.3%). Comparison with past surveys showed that 12 species identified in the past surveys did not appear in this survey; nine species appeared for the first time in this survey; and the ecosystem disturbance species - M. salmoides and L. macrochirus - tended to spread more widely gradually.

• Journal of the Korean Institute of Intelligent Systems
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• v.21 no.5
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• pp.667-672
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• 2011

This paper presents to study the sensor fusion of positioning sensors using UKF(unscented Kalman filter) for positioning accuracy improvement of AGV(automatic guided vehicle). The major guidance systems for AGV are wired guidance and magnetic guidance system. Because they have high accuracy and fast response time, they are used in most of the FMS(flexible manufacturing system). However, they had weaknesses that are high maintenance cost and difficult of existing path modification. they are being changed to the laser navigation in recent years because of those problems. The laser navigation is global positioning sensor using reflecters on the wall, and it have high accuracy and easy to modify the path. However, its response time is slow and it is influenced easily by disturbance. In this paper, we propose the sensor fusion method of the laser navigation and local sensors using UKF. The proposed method is improvement method of accuracy through error analysis of sensors. For experiments, we used the axle-driven forklift AGV and compared the positioning results of the proposed method with positioning results of the laser navigation. In experimental result, we verified that the proposed method can improve positioning accuracy about 16%.