• 생명과학회지
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• 제20권12호
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• pp.1859-1866
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• 2010

우리나라는 세계 녹용 생산량의 80% 이상을 소비한다. 하지만, 중국산, 뉴질랜드산 녹용과 수입이 금지된 북미산 녹용이 원용이라고 불리는 고가의 러시아산 녹용으로 둔갑 판매, 유통되는 문제가 다수 보고되고 있다. 따라서 본 연구에서는 녹용의 원산지 동정 기술을 개발하고자 러시아, 중국, 뉴질랜드 및 북미산 녹용으로부터 미토콘드리아 D-loop 영역에 대한 원산지 특이적인 분자 마커를 탐색할 목적으로 수행되었다. 결과적으로 중국산 녹용으로부터 약 60~70 bp의 결실 부위를 확인하고 이들 부위를 특이적으로 확인할 수 있는 PCR법을 통해서 중국산 녹용의 정확한 감별 가능성을 확인하였다. 따라서 본 연구는 미토콘드리아 DNA 유래의 유전자들에 대한 염기서열 분석과 이를 이용한 PCR 동정법이 녹용의 원산지 감별에 적용될 수 있음을 보여주는 사례라 생각된다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권21호
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• pp.9283-9289
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• 2014

Background: Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents approximately 25% of cancer diagnoses among those younger than 15 years of age. Materials and Methods: This study investigated alterations in the displacement loop (d-loop) region of mitochondrial DNA (mtDNA) as a risk factor and diagnostic biomarker for early detection and diagnosis of acute lymphoblastic leukemia. Using mtDNA from 23 subjects diagnosed with acute lymphoblastic leukemia, the first 450 bp of the d-loop region were amplified and successfully sequenced. Results: This revealed 132 mutations at 25 positions in this region, with a mean of 6 alterations per subject. The d-loop alterations in mtDNA in subjects were all identified as single nucleotide polymorphisms in a homoplasmic distribution pattern. Mutant alleles were observed in all subjects with individual frequency rates of up to 95%. Thirteen mutant alleles in the d-loop region of mtDNA occurred with a high frequency. Novel alleles and locations were also identified in the d-loop of mtDNA as follows: 89 G insertions (40%), 95 G insertions (13%), 182 C/T substitutions (5%), 308 C insertions (19%), and 311 C insertions (80%). The findings of this study need to be replicated to be confirmed. Conclusions: Further investigation of the relationship between mutations in mitochondrial d-loop genes and incidence of acute lymphoblastic leukemia is recommended.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2006년도 춘계학술대회논문집
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• pp.250-253
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• 2006

The in-pile Section (IPS) is subjected to flow-induced vibration(FIV) due to the flow of the primary coolant and then the structural integrity. The in-pile Section (IPS) of 3-pin Fuel Test Loop(FTL) shall be installed in the vortical hole call IR1 of HANARO reactor core. In order to verify the velocity and displacement both the inside region of IPS at the annular region of IPS, the vibration was measured by varing the flow rate on both regions. The displacements of fuel assembly in the in-pile Section (IPS) were found to be lower than the values of allowable design criteria.

• Genomics & Informatics
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• 제18권3호
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• pp.32.1-32.7
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• 2020

The mitochondrial genome of a species is an essential resource for its effective conservation and phylogenetic studies. In this article, we present sequencing and characterization of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba collected from Khulna region of Bangladesh. The complete mitochondrial genome was 16,597 bp in size, which formed a circular double-stranded DNA molecule containing a total of 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes) with two non-coding regions, an origin of light strand replication (OL) and a displacement loop (D-loop), similar structure with other fishes of Teleostei. The phylogenetic tree demonstrated its close relationship with labeonine fishes. The complete mitogenome of Cirrhinus reba (GenBank no. MN862482) showed 99.96% identity to another haplotype of Cirrhinus reba (AP013325), followed by 90.18% identity with Labeo bata (AP011198).

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 1995년도 춘계학술대회 논문집
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• pp.40-62
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• 1995

A micro positioning system using piezoelectric actuators have very wide application region such as ultra-precision machine tool optical device measurement system. In order to keep a high precision displacement resolution it to useful to take a position sensor and feedback of the error. From the practical point of view high-resolution displacement sensor systems are very expensive and it is difficult to make such a sensitive sensor work properly in a poor operational environment of industry. In this study a piezo-electric micro-depth control system which does not require position sensor but piezoelectric voltage feedback has been developed. It is driven by hysteresis-considering reference input voltage calculated in advance and actuator/sensor characteristics of piezoelectric materials. From the result of experiments a fast and stable response of micro-depth control system has been achieved and an efficient technique to control the piezoelectric actuator suggested.

• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1484-1490
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• 2004

Mitochondrial DNA haplotypes from the displacement-loop (D-loop) region (436 bp) were genotyped and sequenced in Japanese Black beef cattle raised in the same herd. Correlation coefficients between mitochondrial DNA haplotypes, maternal lineage, birth weight, preweaning average daily gain, weaning weight, post weaning average daily gain and yearling weight were computed. The objective was to study the relationship between maternal and postnatal growth traits and to investigate if postnatal growth of calves to yearling age could be accurately predicted from mitochondrial DNA haplotypes. Results of the phylogenetic analysis revealed 17 maternal lineages and four mitochondrial DNA haplotypes. There were strong, positive and highly significant (p<0.001) correlations among maternal traits ranging from 0.52 to 0.98. Similarly, among postnatal growth traits, most of the correlations were also strong, positive and highly significant (p<0.001); the highest correlation of 0.94 was between preweaning average daily gain and weaning weight. However, correlations between mitochondrial DNA haplotypes and postnatal growth traits were very low, mostly negative and non-significant (p>0.05) ranging from -0.05 to 0.1. Prediction of postnatal growth from mitochondrial DNA yielded very low $R^{2}$ values ranging from 0.002 to 0.019. It was concluded that mitochondrial DNA polymorphism has no significant association with postnatal growth from birth to yearling age, and by implication, nuclear rather than cytoplasmic DNA, accounts for most of the genetic variation observed in postnatal growth of Japanese Black cattle. Therefore, mitochondrial DNA genotyping at an early age has no bearing on the accurate prediction of the future growth performance of calves.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2006년도 추계학술대회논문집
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• pp.515-518
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• 2006

A vibration test for a cylindrical rod inserted on the grid support structure was tested using the sine sweep excitation method with closed loop force control. The effect of the mounting location of a test rod on the vibration characteristics of a rod continuously supported by the full size($16{\times}16$) grid support was identified. An electromagnetic vibration shaker, non-contact displacement sensor and HP/VXI data acquisition device were used and TDAS software was also used as a data sampling and processing tools. The natural frequencies and mode shape of the test rod were consistent with the previous works of a rod vibration test with partial grids($3{\times}3,\;5{\times}5\;and\;7{\times}7$). The frequency characteristics of the rod according to the mounting location were shown clear discrepancies, but mode shapes were nearly same. As the test rod closes to the bottom clamping region of the spacer grid, peak vibration amplitudes of the rod become smaller.

• 한국정밀공학회지
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• 제12권2호
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• pp.48-58
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• 1995

A micro positioning system using piezoelectric actuators have very wide application region such as ultra-precision machine tool, optical device, measurement systen. In order ro keep a high precision displacement resolution, they use a position sensor and feedback the error. From the practical point of view, a high-resolution displacement sensor system are very expensive and difficult to guarantee such sensitive sensors work properly in the hard opera- tion environment of industry. In this study, a micro-positioning grinding table which does not require position sensor but uses piezoelectric voltage feedback, has been developed. It is driven by hystersis-considering reference input voltage which calculated from computer and then uses actuator/sensor characteristics of piezoelectric materials. From the result of experiments we proved a fast and stable response of micro-positioning system and suggested efficient technique to control the piezoelectric actuator. And through grinding experiments, it is revealed that a characteristics of ground surfaces transient to plastic deformation as extremely small depth of grinding.

• 한국정밀공학회지
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• 제19권9호
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• pp.172-178
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• 2002

One of the ever-increasing demands on the performances of heterodyne interferometers is to improve the measurement resolution, of which current state -of-the-art reaches the region of sub-nanometers. So far, the demand has been met by increasing the clock speed that drives the electronics involved fur the phase measurement of the Doppler shift, but its further advance is being hampered by the technological limit of modem electronics. To cope with the problem, in this investigation, we propose a new scheme of phase -measuring electronics that reduces the measurement resolution without further increase in clock speed. Our scheme adopts a super-heterodyne technique that lowers the original beat frequency to a level of 1 MHz by mixing it with a stable reference signal generated from a special phase- locked-loop. The technique enables us to measure the phase of Doppler shift with a resolution of 1.58 nanometer at a sampling rate of 1 MHz. To avoid the undesirable decrease in the maximum measurable speed caused by the lowered beat frequency, a special form of frequency up-down counting technique is combined with the super-heterodyning. This allows performing required phase unwrapping simply by using programmable digital gates without 2n ambiguities up to the maximum velocity guaranteed by the original beat frequency.

• 한국구조물진단유지관리공학회 논문집
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• 제21권1호
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• pp.1-8
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• 2017

형상비(M/VD, shear span-depth ratio)가 4.5인 축소모형의 원형기둥 실험체 3개를 제작하였다. 철근콘크리트 기둥 실험체의 단면은 원형이고 중공단면으로 제작되었다. 철근콘크리트 기둥 실험체의 단면 지름은 400 mm, 중공 지름은 200 mm이다. 일정한 축력 하에서 반복하중을 가력하는 준정적 실험을 수행하였다. 실험체의 주요변수는 횡방향철근비이다. 모든 실험체의 횡방향 나선철근 체적비는 소성힌지 구간에서 0.302~0.604%의 값을 갖는다. 이 값은 도로교설계기준에서 요구하는 최소 심부구속철근 요구량의 45.9~91.8%에 해당하며, 이는 내진 설계가 되지 않은 기존 교각이나 내진설계개념으로 설계되는 교각을 나타낸다. 본 연구의 최종목적은 실험적 기초자료의 제공과 함께 성능단계별 균열거동, 하중-변위 이력곡선, 에너지 소산 능력, 등가점성감쇠비, 잔류변형, 유효강성 등 내진성능의 정량적 수치와 경향을 제공하기 위한 것이다. 본 논문에서는 실험결과를 통해 분석된 실험변수에 따른 실험결과들을 공칭강도, 비선형 모멘트-곡률 해석 결과, AASHTO LRFD 및 도로교설계기준(한계상태설계법)과 같은 기준들과 비교하였다.