• Childhood Kidney Diseases
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• v.7 no.2
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• pp.217-222
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• 2003

Chronic granulomatous disease(CGD) is a rare inherited disorder of phagocytic cells which results in a susceptibility to infections by catalase-positive bacteria and fungi, as well as granuloma formation. And acute poststreptococcal glomerulonephritis(APSGN) is one of the most common glomerular lesions of gross hematuria in children. We experienced a case of APSGN accompanied with CGD presenting with a liver granuloma.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.13
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• pp.5219-5223
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• 2015

Helicobacter pylori (H. pylori) infection as a serious problem in both adults and children can induce chronic gastritis, peptic ulcer disease (PUD), and possibly gastric cancer. The aim of the current study was to survey antibiotic resistance and also to determine influence of PPAR$\gamma$ polymorphism in patients with H. pylori infection. During an 11-month-period, 98 H. pylori isolates were collected from 104 biopsy specimens. In vitro susceptibility of H. pylori isolates to 4 antimicrobial agents metronidazole, clarithromycin, amoxicillin and tetracycline were assessed by quantitative method according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) guideline. PPAR$\gamma$ polymorphism was determined using polymerase chain reaction-restriction fragment length polymorphism assay. The frequency of H. pylori infection in our study was 94.2%. In vitro susceptibility data showed that highest level of resistance was related to metronidazole (66.3%), and the majority of H. pylori isolates were highly susceptible to amoxicillin and tetracycline (94.9% and 96.9%, respectively). Genotypic frequencies were 25.5% for CC (Pro12Pro), 40.8% for GC (Pro12Ala) and 33.7% for GG (Ala12Ala). In our study, CG genotype had highest distributions among infected patients with H. pylori. The study suggests that the PPAR-$\gamma$ Pro12Ala polymorphism could be evaluated as a potential genetic marker for susceptibility to gastric cancer in the presence of H. pylori infection.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.20
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• pp.8775-8778
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• 2014

Background: To explore the relationship between polymorphisms of interleukin17 (IL-17) gene(A-832G 7488A/G) and the susceptibility to silicosis, a risk factor for lung cancer. Materials and Methods: A total of 113 silicosis patients and 116 workers without silicosis were enrolled in the case-control study. IL-17A A-832G and IL-17F 7488A/G polymorphisms were evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The frequencies of AA,GG and AG of IL-17A A-832G locus in the case and control groups were 46.9%, 8.0%, 45.1%, and 49.2%, 7.6%, 43.2%, respectively, with no significant differences (p>0.05).The GG genotype in the IL-17F (7488A/G) locus was not found. The frequencies of AA and GA of IL-17F 7488A/G locus in the case and control groups were 84.1%, 15.9% and 66.4%, 33.6%, respectively (p<0.05). Analysis of combined effects showed that the individuals with GG+AG genotype of IL-17A and GG+GA genotype of IL-17F are protected against silicosis (OR=0.469). Conclusions: IL-17F 7488A/G is associated with susceptibility to silicosis, and G allele may have a protective effect. No relationship was found between IL-17A gene polymorphisms at A-832G and silicosis.

• Journal of Life Science
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• v.23 no.7
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• pp.919-925
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• 2013

Human thymic stromal lymphopoietin receptor (TSLPR) might play an important role in the development of inflammatory and allergic responses. We previously identified eleven single nucleotide polymorphisms (SNPs) and two variation sites in the TSLPR gene and showed that all the SNPs of the TSLPR gene are associated with susceptibility to atopic asthma. The present study aimed to investigate whether the TSLPR gene SNPs are associated with susceptibility to rheumatoid arthritis (RA). We compared the genotype and the allele frequencies of the TSLPR SNPs in 457 RA patients and 570 healthy controls. The genotype and the allele frequencies of the TSLPR gene SNPs in the RA patients were not significantly different from the respective frequencies of the healthy controls. Additional analysis showed that the genotype and the allele frequencies of the TSLPR gene SNPs did not appear to be associated with RA in female RA patients. The TSLPR gene SNPs in the RA patients did not affect the production of rheumatoid factor (RF) and antisynthetic cyclic citrullinated peptide (CCP). Our results suggest that the TSLPR gene SNPs are not associated with susceptibility to RA in the Korean population.

• Tuberculosis and Respiratory Diseases
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• v.82 no.2
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• pp.143-150
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• 2019

Background: The purpose of this study was to analyze the relationship between the gene mutation patterns by the GenoType MTBDRplus (MTBDRplus) assay and the phenotypic drug susceptibility test (pDST) results of isoniazid (INH) and prothionamide (Pto). Methods: A total of 206 patients whose MTBDRplus assay results revealed katG or inhA mutations were enrolled in the study. The pDST results were compared to mutation patterns on the MTBDRplus assay. Results: The katG and inhA mutations were identified in 68.0% and 35.0% of patients, respectively. Among the 134 isolated katG mutations, three (2.2%), 127 (94.8%) and 11 (8.2%) were phenotypically resistant to low-level INH, high-level INH, and Pto, respectively. Among the 66 isolated inhA mutations, 34 (51.5%), 18 (27.3%) and 21 (31.8%) were phenotypically resistant to low-level INH, high-level INH, and Pto, respectively. Of the 34 phenotypic Pto resistant isolates, 21 (61.8%), 11 (32.4%), and two (5.9%) had inhA, katG, and both gene mutations. Conclusion: It is noted that Pto may still be selected as one of the appropriate multidrug-resistant tuberculosis regimen, although inhA mutation is detected by the MTBDRplus assay until pDST confirms a Pto resistance. The reporting of detailed mutation patterns of the MTBDRplus assay may be important for clinical practice, rather than simply presenting resistance or susceptibility test results.

• Korean Journal of Agricultural Science
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• v.47 no.3
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• pp.693-705
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• 2020

Grapes (Vitis spp. L.) are the third most produced fruit in the world. Crown gall disease caused by Agrobacterium vitis forms galls in the stems of the grapevines and reduces the vitality of the fruit trees, resulting in reduced yields. This pathogen has occurred in vineyards worldwide and caused serious economic losses. It is a soil-borne disease, so Agrobacterium vitis can survive for several years in vineyards and is difficult to control. Additionally, since there is no effective chemical control method, the most effective control method is the breeding of resistant varieties. To make the resistant variety, marker-assisted selection (MAS) enables fast breeding with low cost. In this study, we applied a genome-wide association study (GWAS), by combining phenotyping and genotyping-by-sequencing (GBS), for the development of a single nucleotide polymorphism (SNP) marker set related to crown gall disease using 350 grapevine varieties. As a result of the GBS based genotyping analysis, about 58,635 SNPs were obtained. In addition, the phenotypic analysis showed 35.2% resistance, 73% moderate susceptibility and 16.4% highly susceptibility. Moreover, after confirmation, two genes (VvARF4 and VvATL6-like) were shown to be related to crown gall disease based on the results of GWAS analysis, using the phenotypic data, and GBS. High-resolution melting analysis (HRMA) was performed using the Luna^® Universal Probe with real-time PCR to distinguish the melting peaks of the resistant and susceptible varieties. Our data show that these SNP markers are expected to be helpful in evaluating resistance against grapevine crown gall disease and in breeding.

• Journal of Korean Academy of Nursing
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• v.13 no.3
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• pp.61-74
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• 1983

The proportion of people who contacted pulmonary T.B. in Korea has drastically decreased as a result of the incessant effort of the Korean government which adopted a policy of“drive out T.B.”as its foremost health policy. However, the proportion still remains relatively high com-pared with that of developed countries. This study attempts to find some means for guiding and educating college students who have T.B. in their health care by (1) first determining the effect if their self-concept and health beliefs on their behavior in regard to their disease and (2) then predicting the level of compliance of the new patients to the treatment suggested by the health specialist, before the commencement of the treatment. The subjects of this study consisted of 88 mald and female students at Y University who were diagnosed as minimal pulmonary T.B. patients and registered at the health clinic of Y University during the period between September 1, 1981 and March 31, 1953. Data were collected from them by means of questionnaire and interview. The instruments used for this study were (1) a part of Junghoon Choi's“Perceptual Orientation ,Scale”for measuring self-perception of patients and (2) Rosenberg's questionnaire for measuring patients' evaluation of self-esteem, and (3) an instrument for measuring patients' health beliefs which was developed by this researcher utilizing information available from references. The collected data were analyzed using descriptive statistics, chisquare test, Pearson correlation coefficient and t-test. The findings were as follows: 3. Test of hypotheses 1) Hypothesis 1: Patients with high self-concept will be high in health beliefs. For testing this hypothesis a calculation of Pearson correlation coefficient (r) between the patients' self-concept and their health beliefs was carried out. The result of this test was -. 0756 which was not significant at α=.05 and hence hypothesis 1 was not supported. 2) Hypothesis 2: Patients with a high self-concept will tend to be high in compliance with the suggested treatment. Again a Pearson correlaton coefficient was calculated between the two variaibles in the hypothesis. The calculated coefficient r was .1558 which was not significant at α=.05. Hence hypothesis 2 was rejected. 3) Hypothesis 3: Patients with high susceptibility will have a high compliance level. The correlation coefficient between the two variables was -.1975, which was significant at α=.05 but due to the negative sign hypothesis 3 could not be accepted. 4) Hypothesis 4: Patients who take their disease seriously will have a higher compliance level. The calculated correlation coefficient between the variables in this hypothesis was .1642 which was not significant at α=.05 and hence hypothesis 4 was rejected. 5) Hypothesis 5: Patients with a high sense of the benefit of treatment will have a high level of compliance. The computed correlation coefficient was .3129 which was significant at α=.05 and hence hypothesis 5 was acepted. 2. Findings from the correlation analysis were as follows: 1) Patients' susceptibility and their compliance to treatment was negatively correlated (r= -. 1975) which was significant at α= .05. This implies that as the patients' level of susceptibility increases their compliance level decreases. 2) Patients' susceptibility and their self-concept were negatively correlated (r= -. 1790) which was again singnificant at α=.05. The implication of this is that as the patients’self concept increases their susceptibility to disease decreases. 3) Patients' self-concept and their sense of benefit derieved from the treatment was positively correlated (r=.1970) which was significant at α=.05. That is, patients with a high self-concept perceived a great sense of benefit from the treatment. To summarize, patients who are low in susceptibility have a high level of compliance and self-concept.

• Investigative Magnetic Resonance Imaging
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• v.4 no.2
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• pp.113-119
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• 2000

Purpose : To know the possibility of clinical application of MRI using oxygen inhalation as a perfusion MRI Materials and methods : Two healthy volunteers and three patients of one moyamoya disease, one acute infarction and one meningioma were studied using a 1.5 Tesla MRI unit. Oxygen (15 liters/min) mixed with room air was given using face mask from 8 second to 35 second during the study. Images were acquired 25 times (scan time per study were 1.6 seconds) using susceptibility contrast EPI (echo planar image) sequence. Difference maps were acquired by early (study 12-18), and late (study 19-25) O2 inhalation image groups minus pre-O2 inhalation image group (study 3-9) with a Z-score of 0.7-1.0 using VB31C program of Magneton Vision. The resulting perfusion images were created by superimposition of difference maps on corresponding T1 weighted anatomic images. On moyamoya patient, similar perfusion images were acquired after Gd-DTPA injection, and compared with O2 inhalation perfusion images. Results ; The author can get the perfusion images of the brain by oxygen inhalation with susceptibility contrast EPI sequence at the volunteers, and the patient of moyomoya disease, acute infarction and meningioma. On moyamoya patient, perfusion images with O2 inhalation are similar with perfusion images by Gd-DTPA injection. Conclusion 1 This study has demonstrated that the susceptibility contrast EPI by oxygen inhalation can be used as the clinically useful perfusion MRI technique

• Journal of fish pathology
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• v.26 no.3
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• pp.283-287
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• 2013

The susceptibility of marine medaka, Oryzias dancena to fish pathogenic viruses (infectious pancreatic necrosis virus (IPNV), viral hemorrhagic septicemia virus (VHSV), hirame rhabdovirus (HIRRV), infectious hematopoietic necrosis virus (IHNV), and lymphocystis disease virus (LCDV)) was investigated. The cumulative mortalities of fish immersed with IPNV (experimental condition: $15^{\circ}C$ sea water (SW)), VHSV ($15^{\circ}C$ SW), HIRRV ($15^{\circ}C$ fresh water (FW)) were 30%, 40% and 60%, respectively. In the fish immersed with IPNV ($15^{\circ}C$ FW, $18^{\circ}C$ FW and SW), VHSV ($15^{\circ}C$ FW, $18^{\circ}C$ FW and SW), HIRRV ($15^{\circ}C$ SW), IHNV ($15^{\circ}C$ FW and SW), LCDV ($15^{\circ}C$ FW and SW, $18^{\circ}C$ FW and SW), and mock-challenged group, mortality rate was less than 10%. IPNV, VHSV and HIRRV were re-isolated from the dead fish. These results suggest that marine medaka is susceptible to IPNV, VHSV and HIRRV, although their susceptibility depends on the environmental conditions.

• The Korean Journal of Physiology and Pharmacology
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• v.19 no.6
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• pp.479-484
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• 2015

NOD2 (nucleotide-binding and oligomerization domain 2) was initially reported as a susceptibility gene for Crohn's disease, with several studies focused on elucidating its molecular mechanism in the progression of Crohn's disease. We now know that NOD2 is an intracellular bacterial sensing receptor, and that MDP-mediated NOD2 activation drives inflammatory signaling. Various mutations in NOD2 have been reported, with NOD2 loss of function being associated with the development of Crohn's disease and other autoimmune diseases. These results suggest that NOD2 not only has an immune stimulatory function, but also an immune regulatory function. Atherosclerosis is a chronic inflammatory disease of the arterial wall; its pathologic progression is highly dependent on the immune balance. This immune balance is regulated by infiltrating monocytes and macrophages, both of which express NOD2. These findings indicate a potential role of NOD2 in atherosclerosis. The purpose of this review is to outline the known roles of NOD2 signaling in the pathogenesis of atherosclerosis.