• Title/Summary/Keyword: disc-plate method

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Effects of Cactus Extracts on Human Intestinal Microflora (선인장 추출물이 인체 장내미생물에 미치는 영향)

  • Ra, Bo-Hyun;Lee, Woon-Jong;Cho, Yun-Won;Kim, Kwang-Yup
    • Journal of agriculture & life science
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    • v.43 no.3
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    • pp.45-54
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    • 2009
  • This study was designed to investigate the effect of cactus (Opuntia ficus-indica var. saboten) extracts on the intestinal bacteria, antioxidative activity and angiotensin -I-converting enzyme(ACE) inhibitory activity. The antimicrobial activities were measured using the 96well-plate method and disc plate method with concentration of 20mg of cactus extract. The stem extract of cactus was inhibitory against Eubacterium limosum, Clostridium perfringens, C. butyricum, C. difficile and Staphylococcus aureus, but was not inhibitory against Bacteroides fragilis, Bifidobacterium bifidum, Lactobacillus acidophilis, Streptococcus thermophilus. The fruit extract of cactus showed no inhibition against Bacteroides fragilis, Bifidobacterium bifidum, Lactobacillus acidophilis, and Streptococcus thermophilis. Their inhibitory activities were not reduced after heat and pH treatment. Antioxidative effects of cactus extracts showed high total polyphenol and flavonoid contents and high activity against free radical DPPH. The stem and fruit extract of cactus showed strong ACE inhibitory activities of 88.8% and 69.2%, respectively. In conclusion cactus (Opuntia ficus-indica var. saboten) extract might be utilized as a functional food material to control intestinal microflora.

A Study on the Size Evaluation of Disc and Band Type Flaw by Ultrasonic Tandem Testing (초음파(超音波)TANDEM사각법(斜角法)에 의한 원형(圓形) 및 띠형결함(形缺陷)의 크기 평가(評價)에 관한 연구(硏究))

  • Han, E.K.;Eom, H.S.;Kim, J.J.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.5 no.2
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    • pp.12-21
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    • 1986
  • Generally, butt welds with plate thickness $30{\sim}40mm$ are welded with groove angle $40^{\circ},\;60^{\circ},\;70^{\circ}$, etc. In the detection of internal weld defects, oblique testing with single probe has been mainly used. But, recently, in acccordance with enlargement of welded structure, thick plate with 100-200mm are frequently required. Thus I-groove welding method was lately developed and often used. In this case, most frequently generated defects are the lack of weld penetration and incomplete fusion between base metal and welding material. If we would detect by oblique testing with single probe, detecting flaw is occassionally impossible or very underestimated. In this study, the limit for applying tandem method was studied in dise and band type flaws. The estimation of flaw size could be within 10% error compared to real flaws.

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A Computation of the Resonant Frequencies using Contour Graph Method in the Dielectric-disc Loaded Cylindrical Cavity Resonators (유전체 디스크가 삽입된 원통형 공동 공진기에서 Contour Graph 방법을 이용한 공진주파수 계산)

  • Choi, Hong-Ju;Lee, Won-Hui;Hur, Jung;Choi, Gwang-Je;Lee, Sang-Young
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.11 no.8
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    • pp.1420-1427
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    • 2000
  • We described a method to obtain electromagnetic solution and resonant frequencies in dielectric-disc loaded cylindrical cavity resonators. Resonant frequency of dielectric-disc loaded cylindrical cavity resonators is calculated by analyzing the characteristic equation. The characteristic equation is solved by using the ContourPlot graph of Mathematica. As increasing to height of cavity, we compare the calculated resonant frequency with experimental frequency. The calculated results well agree with the experimental ones. The error between them is 0.2% or 1.6% for the case of the top plate is close to or far from concentric dielectric-disc, respectively.

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Outcome Analysis of the Patients with and without Anterior Plating in Multi-Level Degenerative Cervical Diseases (다분절 퇴행성 경추질환에서의 전방 금속판 사용 유무에 따른 환자의 예후분석)

  • Kim, Sang Woo;Kim, Sung Min;Shin, Dong Ik;Cho, Yong Jun;Shim, Young Bo;Choi, Sun Kil
    • Journal of Korean Neurosurgical Society
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    • v.30 no.12
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    • pp.1369-1374
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    • 2001
  • Objectives : The rates of pseudarthrosis for two- and three level fusion have been reported to be 17-63 and 50% without anterior cervical plating. The purpose of this study is to assess the effects of anterior cervical plating in the treatment of multilevel degenerative cervical disease such mostly the additional risks associated with hardware implants and its benefits, fusion rate and radiographic results, and clinical outcomes. Methods : Forty-seven patients who underwent operations between 1993 and 1997 were retrospectively reviewed. The technique for operation was same for both groups(Smith Robinson with autologous iliac bone graft). Group I consisted of 35 consecutive patients treated with anterior cervical decompression and fusion with anterior cervical plate fixation. Group II consisted of 12 consecutive patients treated without plate fixation. We compared clinical outcomes by Prolo score, radiographic results in the rate of fusion, cervical lordosis by Gore angle, disc height by Farfan method, and surgical complications between two groups. Results : The favorable clinical outcomes(excellent and good) by Prolo score were observed with the use of anterior cervical plate fixation(89% vs 75%). The successful fusion rate of multilevel cervical fusion was as seen with anterior cervical plate fixation(97% vs 75%). The overall graft complication rate in multilevel fusions was decreased, with anterior cervical plate fixation, and the hardware related complications were relatively minimal without serious consequences. Conclusion : Anterior cervical plate fixation in the treatment of multilevel cervical disorders is an effective stabilizing method which provides increased bony fusion rate, decreased graft complications, maintained cervical lordosis, early mobilization of the these patients without serious hardware related complications.

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Numerical Simulation of Axisymmetric Supersonic let Impingement on a Flat Plate (수직평판에 충돌하는 축대칭 초음속 제트의 수치 연구)

  • 신완순;이택상;박종호;김윤곤;심우건
    • Journal of the Korean Society of Propulsion Engineers
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    • v.4 no.3
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    • pp.11-18
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    • 2000
  • When supersonic underexpanded jets are exhausted from the nozzle, complex shock cell configurations such as barrel shock, expansion fan, Mach disc, and exhaust-gas jet boundary are appeared repetitively. The shock cell is smeared by turbulence dissipation and disappeared in long distance from the nozzle. When underexpanded jet is suddenly impinged on a flat plate, it forms very complex flow structure. In this paper, we solve compressible Wavier-Stokes equation adapting finite volume method to obtain jet impingement flow structure and compare calculated data with experimental ones. It is shown that numerical simulation data are in good agreement with experimental one in a short distance between nozzle exit and flat plate and little influence of underexpanded ratio is appeared in jet impingement now distribution.

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Effect of various chromatographic terpenoid fractions of Luffa cylindrica seeds on in-vitro antimicrobial studies

  • Nagarajan, K.;Saxena, Pallavi;Mazumder, Avijit;Ghosh, L.K.;Devi, G. Uma
    • Advances in Traditional Medicine
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    • v.10 no.1
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    • pp.21-28
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    • 2010
  • The objective of the present investigation is to evaluate the antimicrobial potency of the terpenoid fractions isolated from Luffa cylindrica seeds against various pathogenic microbes. The seeds were powdered and extracted with methanol in soxhlet appratus based on phytochemical screening. Three terpenoid components were isolated by column chromatography and identified by thin layer chromatography and chemical analysis which were designated as ${LCSF_4}^*$, ${LCSF_6}^*$ & ${LCSF_8}^*$ respectively. Disc diffusion method was employed to determine the antimicrobial effectiveness of test compounds I, II and III $({LCSF_4}^*,\;{LCSF_6}^*\;&\;{LCSF_8}^*)$ against 6 microbial species viz., Staphylococcus (S.) aureus, Bacillus (B.) subtilis, Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, Candida (C.) albicans and Aspergillus niger. The disc was saturated with $100{\mu}l$ of each compound, allowed to dry and introduced on the upper layer of seeded agar plate. The plates were incubated overnight at $37^{\circ}C$. Microbial growth was determined by measuring the zonal inhibition diameters. Compound I showed maximum potency against gram positive S. aureus (21 mm) in comparison with standard ciprofloxacin (38 mm), whereas the same compound was completely devoid of activity against both the fungi tested. Compound II was found to be highly sensitive against both the gram negative E. coli (20 mm) and P. aeruginosa (22 mm). Compound II was found to exhibit maximum potency against the fungi C. albicans (15 mm) and A. niger (20 mm). Compound III was found to be very effective against both the gram positive S. aureus (20 mm) and B. subtilis (15 mm) respectively.

Antibacterial and anti-obesity effects of Abeliophyllum distichum Nakai: an in vitro study

  • Song, Dong Cheol;Lee, Ji Hwan;Oh, Han Jin;Kim, Yong Ju;An, Jae Woo;Chang, Se Yeon;Go, Young Bin;Cho, Hyun Ah;Cho, Jin Ho
    • Korean Journal of Agricultural Science
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    • v.48 no.3
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    • pp.557-565
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    • 2021
  • Interest in research on various medicinal plants has increased globally over the last few decades, possibly due to their possible antibacterial and antioxidant activities. The present study was conducted to verify the antioxidant effects, antibacterial activity, and collagen synthesis and cell viability outcomes of adipocytes upon exposure to Abeliophyllum distichum Nakai (AdN). Antibacterial activity was measured through the Disc diffusion method to compare the growth ability of pathogenic microorganisms (E.coli, Salmonella). The absorbance was measured at 560 nm to calculate the active oxygen scavenging ability. Fibroblasts were dispensed in a 96-well plate at a density of 1 × 105 cells·well-1. The amount of procollagen was measured in each case using a procollagen type 1 C-peptide EIA KIT. The cytotoxicity of the Abeliophyllum distichum Nakai extract against animal adipocytes (Hanwoo backfat cells) was determined using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, a method that measures the conversion of MTS to Formazan by means of mitochondrial dehydrogenases. The concentrations of the samples were made to be 0.0125, 0025, 0.05, 0.1, and 0.5% and all were -completely absorbed into the disc in an incubator at 37℃ for 24 to 36 hours. For the 0.125 mg·disc-1, effects of Abeliophyllum distichum Nakai on the antioxidant effect, antibacterial activity, and cell viability of adipocytes were found. However, Abeliophyllum distichum Nakai had no effect on collagen synthesis, thus suggesting that AdN extracts may be useful for the prevention and/or treatment of obesity.

Compensation Method of Mechanical Error for Micro Holographic Storage (마이크로 홀로그래픽 저장장치의 기계적 에러 보상기법)

  • Min, Cheol-Ki;Jeon, Sung-Bin;Cho, Chang-Hyun;Kim, Do-Hyung;Park, No-Cheol;Yang, Hyun-Seok;Park, Kyoung-Su;Park, Young-Pil
    • Transactions of the Society of Information Storage Systems
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    • v.8 no.1
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    • pp.11-15
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    • 2012
  • In this paper, we experimentally investigated compensation method of the mechanical errors for the micro holographic storage. The aim of this research was overcome of disc tilt and spherical aberration problems. The proposed method was performed by using glass plate and zoom optics.

Long-term Effects on the Cervical Spine after Anterior Locking Plate Fixation (경추 전방 잠금식 금속판이 장기적으로 경추에 미치는 영향)

  • Kim, Keun Su
    • Journal of Korean Neurosurgical Society
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    • v.30 no.4
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    • pp.493-500
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    • 2001
  • Objective : Anterior cervical locking plates are the devices for achieving anterior cervical spinal fusion. This study was conducted to evaluate the locking plate system regarding its long-term advantages and disadvantages in the view of interbody fusion rate, hardware-related failures, vertebral change close to the fusion segment and postoperative complications. Method : Eight-six patients, operated from Jan., 1996 to Jun. 1998, were followed-up for more than two years. All of the cases were fused with iliac bone graft and ORION locking plate(Sofamor Danek USA, Inc., Memphis, TN) fixation. The patients were discharged or transferred to rehabilitation department 2-7 days after operation. A comprehensive evaluation of the interbody fusion state, instrument failure, vertebral change and postoperative complications were made by direct interview and cervical flexion-extension lateral plain films. Results : There were 55 male and 31 female with a mean age of 45 years(18-75 years). The mean follow-up period was 29 months(24-43 months). Various disorders that were operated were 40 cervical discs, 6 cervical stenosis including OPLL, 2 infections, and 38 traumas. Fusion level was single in 59 cases, two levels of each disc space in 15 cases, and two levels after one corpectomy in 12 cases. There was no instrument failure. Pseudoarthrosis was observed in two cases(2%) without radiological instability. The other patients(98%) showed complete cervical fusion with stable instrument. Mild settling of interbody graft with upward migration of screws was found in 12 cases(14%). Anterior bony growth at the upper segment was found in 5 cases(6%). Postoperative foreign body sensation or dysphagia was observed in 12 cases(4%), and disappeared within one month in 7 cases and within six months in 4 cases. One patient complained for more than six months and required reoperation to remove paraesophageal granulation tissue. Conclusion : The results show that Orion cervical locking plate has some disadvantages of upward migration of screws, anterior bony growth at the upper segment, or possibility of esophageal compression even though it has advantages of high interbody fusion rate or low instrument failure. Author believe that anterior cervical locking plate in the future should be thinner, and should have short end from the screw hole, and movable screw with adequate stability.

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Root cause analysis of sticking in hydraulically actuated multi-disc friction clutch for ship propulsion (선박 추진용 유압작동식 다판 마찰클러치 고착현상 고장탐구)

  • Jeong, Sang-Hu;Kim, Jeong-Ryeol;Shin, Jae-Won
    • Journal of Advanced Marine Engineering and Technology
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    • v.41 no.4
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    • pp.330-336
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    • 2017
  • This study performs a root cause analysis of the sticking that occurs in the hydraulically actuated wet type multi-disc friction clutch in a ship's diesel engine propulsion system that uses rubber elastic coupling. The fishbone method was used to study the sticking through dismantling investigation of the reduction gear and clutch, investigation of the components, and onboard system tests including nondestructive testing. The friction plate sticking is caused by the slip due to friction heat resulting from the leakage of control oil through cracks in the assembled hollow shaft. The friction plate cooling oil also leaks simultaneously through the crack, and partial sticking occurs due to the hot spots in the friction plates. These are caused by insufficient amount of cooling oil due to oil leakage.