• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1470-1474
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• 2009

This paper describes the development a of direct multiplex reverse transcription-nested polymerase chain reaction (PCR) method, devised for simultaneous detection and typing of influenza viruses. This method combines the direct reverse transcription reaction without RNA purification with the enhancement of sensitivity and specificity of nested PCR. The method successfully detected three major human influenza viruses: influenza virus A subtype 1 (H1N1) and subtype 3 (H3N2), and influenza B virus (B). The minimum number of virus particles (pfu/ml) necessary for detection in spiked saliva samples was 200 (H1N1), 140 (H3N2), and 4.5 (B). The method's sensitivity and simplicity will be convenient for use in clinical laboratories for the detection and subtyping of influenza and possibly other RNA viruses.

• Molecules and Cells
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• 제21권3호
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• pp.376-380
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• 2006

Gene expression is regulated in large part at the level of transcription under the control of sequence-specific transcriptional regulatory proteins. Therefore, the ability to affect gene expression at will using sequencespecific artificial transcription factors would provide researchers with a powerful tool for biotechnology research and drug discovery. Previously, we isolated 56 novel sequence-specific DNA-binding domains from the human genome by in vivo selection. We hypothesized that these domains might be more useful for regulating gene expression in higher eukaryotic cells than those selected in vitro using phage display. However, an unpredictable factor, termed the "context effect", is associated with the construction of novel zinc finger transcription factors--- DNA-binding proteins that bind specifically to 9-base pair target sequences. In this study, we directly selected active artificial zinc finger proteins from a zinc finger protein library. Direct in vivo selection of constituents of a zinc finger protein library may be an efficient method for isolating multi-finger DNA binding proteins while avoiding the context effect.

• Journal of Astronomy and Space Sciences
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• 제28권4호
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• pp.285-290
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• 2011

This paper presents an algorithm that can provide initial conditions for formation flying at the beginning of a region of interest to maximize scientific mission goals in the case of a tetrahedral satellite formation. The performance measure is to maximize the quality factor that affects scientific measurement performance. Several path constraints and periodicity conditions at the beginning of the region of interest are identified. The optimization problem is solved numerically using a direct transcription method. Our numerical results indicate that there exist an optimal configuration and states of a tetrahedral satellite formation. Furthermore, the initial states and algorithm presented here may be used for reconfiguration maneuvers and fuel balancing problems.

• BMB Reports
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• 제29권3호
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• pp.221-224
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• 1996

To examine the growth-phase dependent control of Escherichia coli rnpB gene we used a combination of Northern analysis for RNA determination and Southern analysis for plasmid DNA determination. The relative amounts of metabolically unstable transcript derived from the internally deleted rnpB gene harbored on a multicopy plasmid as well as the relative plasmid contents were measured by Northern analysis and Southern analysis, respectively, of total nucleic acids from E coli cells containing the plasmid. The relative transcription activity of the rnB was represented by a ratio of the relative amount of the transcript to that of the plasmid DNA during bacterial growth. The rnpB transcription increased rapidly with time during exponential growth, but started to decrease before the transition period of an exponential growing cell culture into the stationary phase. Although the expression pattern was similar to the changes of ${\beta}-galactosidase$ activity expressed from the lysogenic strain carrying the chromosomal rnpB-lacZ fusion which were shown in a previous work, the present data appears to represent a more actual growth-phase control of the rnpB transcription than the previous data by the ${\beta}-galactosidase$ assay. In addition the present method described for a direct analysis of both RNA and plasmid DNA provides a rapid and efficient method that can applied to an examination of transcription control by using a multicopy plasmid.

• BMB Reports
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• 제30권3호
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• pp.234-236
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• 1997

Chronic hepatitis C virus (HCV) infection is associated with the rapid development of cirrhosis and hepatocellular carcinoma. It has been reported that the amount of HCV RNA may be correlated with the progression of hepatitis and may be a prognostic marker for treatment of HCV patients. The direct detection of HCV RNA by reverse transcription-polymerase chain reaction (RT-PCR) is widely used to determine the presence of circulating virions. The most relevant limit of this approach is the lack of quantitative information about the viral titer. In the present study, we developed the method for HCV quantitation using competitive reverse transcription (CRT)-PCR using the deleted HCV standard. The serially diluted standard was added in titrated amounts to the target HCV RNA. The mixture was then reverse transcribed and amplified in the same reaction tube. The methods were evaluated using over 110 HCV-PCR positive samples in Koreans. About 59% of the samples were judged to contain $10^{5}-10^{6}$ copies of HCV RNA in 1 ml of serum.

• 한국항공우주학회지
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• 제38권7호
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• pp.684-692
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• 2010

본 논문에서는 지구-달 천이를 위한 최적 궤도 설계에 관한 연구를 수행하였다. 지구와 달의 인력을 동시에 고려한 평면상 제한 3체 궤도 운동 모델을 바탕으로 지구 출발시에는 순간 추력을, 지구-달 천이 과정 및 달 임무궤도 투입시에는 연속 추력을 사용하는 혼합형 궤도전이 방법을 제시하였다. 최적화 풀이 방법으로서 Direct Transcription 및 Collocation을 이용한 비선형 프로그래밍 기법을 적용하였으며, 지구 출발 및 달 임무궤도 투입 궤적의 형상은 순간 추력의 연속 추력에 대한 상대 가중치 및 비행시간에 의하여 매우 달라질 수 있음을 파악하였다.

• 접착 및 계면
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• 제24권4호
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• pp.120-123
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• 2023

우수한 기계적, 전기적 특성을 지닌 그래핀은 기존 재료보다 우수한 물성을 가지고 있기 때문에 전세계의 많은 연구자들에게 각광을 받고 있다. 이러한 그래핀을 전자소자에 응용하기 위해서는 전사 과정 및 패터닝 공정이 반드시 필요하나, 이 과정에서 무수한 결함이 발생되어 그래핀의 특성을 크게 저하시킨다는 문제점이 있다. 그래핀의 우수한 특성 및 상용화를 위해 전사 과정 및 패터닝 공정을 한 번에 진행할 수 있는 공정 개발이 다양한 시도를 통해 행해지고 있다. 본 연구에서는 고분자 나노와이어를 마스크로 사용하여 정밀한 패턴과 동시에 그래핀이 직성장할 수 있는 새로운 성장법을 개발하였다. 개발된 새로운 성장법을 통해 미래의 나노소재 기반 우수한 전자소자를 구현할 수 있을 것이라 기대된다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2003년도 추계학술대회논문집
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• pp.221-224
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• 2003

Recently, the market share of the thin-film-transistor liquid-crystalline-display (TFT-LCD) is growing rapidly in display device market. The backlight unit is used as a light source of TFT-LCD module. A light-guide is one of several important components of backlight unit. The manufacturing technology and optical system design of the light guide is very sensitive to quality and cost of the TFT-LCD module. In the present study a new manufacturing method which is called as direct surface forming(DSF) has been tested under various conditions. The result of this test, V-groove pattern shows different shapes depends on the temperature of mold surface, contact time of mold and depth of V-groove.

• Journal of Microbiology and Biotechnology
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• 제16권3호
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• pp.355-359
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• 2006

The functional stability of mRNA is one of the crucial factors affecting the efficiency of in vitro translation. As the rapid degradation of mRNA in the cell extract (S30 extract) causes early termination of the translational reactions, extending the mRNA half-life will improve the productivity of the in vitro protein synthesis. Thus, a simple PCR-based method is introduced to increase the stability of mRNA in an S30 extract. The target genes are PCR-amplified with primers designed to make the ends of the transcribed mRNA molecule anneal to each other. When compared with normal mRNA, the mRNA with the annealing sequences resulted in an approximately 2-fold increase of protein synthesis in an in vitro translation reaction. In addition, sequential transcription and translation reactions in a single tube enabled direct protein expression from the PCR-amplified genes without any separate purification of the mRNA.

• 식물병연구
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• 제21권4호
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• pp.315-320
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• 2015

Soybean mosaic virus(SMV)는 potyvirus 속에 속하며, 모자이크, 괴사, 기형 등의 병징을 야기하고 국내에서는 11개 계통(G1 to G7, G5H, G6H, G7H, G7a)이 보고되어있다. Reverse transcription loop-mediated isothermal amplification(RT-LAMP) 방법은 등온에서 유전자 증폭이 가능하게 하며, 이 방법은 PCR 과정이나 전기영동 없이도 바이러스에 감염된 식물을 검출할 수 있는 이점이 있다. RT-LAMP의 최적반응 조건은 $58^{\circ}C$, 60분으로 확인되었다. 특이성 검정을 위해 콩에서 발생하는 여러 바이러스들과 보유중인 SMV의 9 계통에서 그 특이성을 확인하였다. 그 결과 SMV에 대한 RT-LAMP primer들의 종 특이성이 확인되었으며, SMV의 계통들에 대해서도 적용이 가능한 것으로 확인되었다. 항온수조와 heating block과 같은 간편한 등온 장치에서 재현성을 확인하기 위해 Thermocycler 기기와 비교하여 증폭 여부를 확인한 결과 반응의 차이는 나타나지 않았다. RTLAMP 반응 이후, 반응물을 전기영동과 SYBR Green I을 이용하여 자연광과 UV광에서 증폭 여부를 확인하였다. 그 결과 전기 영동, 자연광, portable UV light와 UV transilluminator에서 모두 반응이 확인되었다.