• Microbiology and Biotechnology Letters
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• v.20 no.4
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• pp.437-444
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• 1992

An innovative process for high fructose corn syrup (HFCS) production coupled with direct saccharification of raw corn starch in the agitated bead enzyme reactor (bioattitor) was investigated. The required high concentration/purity of glucose solution suitable for isomerization was produced directly in a bioattritor. without condensation of hydrolyzate, 398 g glucose/$\ell$ and 98% glucose content from 400 g/$\ell$ (w/v) of raw corn starch after 24 hours. The unsaccharified residual starch could be separated easily upon centrifugation, and resaccharified. The obtained solution also possessed other desirable requirements as substrate for isomerization, such as. low concentrations of denatured protein and calcium ions, thereby, simplified the purification step. The obtained glucose solution was isomerized in an enzyme reactor paked with immobilized glucose isomerase to evaluate the suitability as a substrate. The proposed new HFCS process seems to have many advantages over the conventional process via liquefaction-saccharification steps. The follow-up investigations of the proposed process need to be conducted to evaluate the feasibility of industrial application.

• Microbiology and Biotechnology Letters
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• v.21 no.2
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• pp.157-162
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• 1993

Raw starch can be effectively saccharified in an enzyme reaction system containing sttrition-milling media. In order to develop an effcient attrition-coupled bioreactor(bioattritor), a rotating drum type bioattitor was construced, and its optimal operation conditions and power consumptions were evaluated. The optimal conditions for 3l bioattritor were 4 baffled, baffle size of 1:0.05 (the ratio of drum diameter to baffle), drum rotation speed of 100 rpm, and 1.33g of 3 mm glass bead/g of raw corn starch.