• Title/Summary/Keyword: direct heat method

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Bacillus polyfermenticus CJ9, Isolated from Meju, Showing Antifungal and Antibacterial Activities (메주로부터 분리한 항진균 및 항세균 활성의 Bacillus polyfermenticus CJ9)

  • Jung, Ji-Hye;Chang, Hae-Choon
    • Microbiology and Biotechnology Letters
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    • v.37 no.4
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    • pp.340-349
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    • 2009
  • A CJ9 bacterial strain, which showed antifungal and antibacterial activities, was isolated from meju and identified as Bacillus polyfermenticus based on Gram staining, biochemical properties, as well as its 16S rRNA sequence. B. polyfermenticus CJ9 showed the antimicrobial activity against the various pathogenic molds, yeasts, and bacteria. The antibacterial activity was stable in the pH 5.0~9.0, but the activity was lost at $37^{\circ}C$ for 24 hr. The antifungal activity was stable in the pH range of 3.0~9.0 and reduced at $121^{\circ}C$ for 15 min, but antifungal activity was not completely destroyed. The antibacterial activity was completely inactivated by proteinase K, protease, trypsin, and $\alpha$-chymotrypsin. The antifungal activity was also completely inactivated by protease and $\alpha$-chymotrypsin, and reduced its activity by proteinase which indicated that the antifungal and antibacterial compounds have proteineous nature. The apparent molecular mass of the partially purified antifungal compound, as indicated by using the direct detection method in Tricine-SDS-PAGE, was approximately 1.4 kDa. The molecular mass of the antibacterial compound could not be determined because of its heat-liable characteristic.

Characterization of Bacteriocin-Like Substances Produced by Bacillus subtilis MJP1 (Bacillus subtilis MJP1이 생산하는 Bacteriocin-Like Substances)

  • Yang, Eun-Ju;Chang, Hae-Choon
    • Microbiology and Biotechnology Letters
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    • v.35 no.4
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    • pp.339-346
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    • 2007
  • The MJP1 bacterial strain, which possesses antifungal activity, was isolated from meju and identified as Bacillus subtilis based on its morphological and biochemical properties, as well as its 16S rRNA sequence. Antimicrobial activity was found against various species of Gram-positive bacteria, yeasts, and molds, including food-spoilage microorganisms. The antifungal activity was found to be stable after heat and proteolytic enzyme treatment, and in the pH range of $6.0{\sim}10.0$. The antibacterial activity was stable in the pH range of $6.0{\sim}10.0$, but about 50% of the activity was lost after 24 hr at $30^{\circ}C$. The antibacterial compound was also inactivated by proteolytic enzyme treatment, indicating its proteinaceous nature. The apparent molecular masses of the partially purified antifungal and antibacterial compounds, as indicated by using the direct detection method in Tricine-SDS-PAGE, were approximately 2.4 kDa and 4.5 kDa, respectively. These studies suggest that B. subtilis MJP1 produces two bacteriocin-like substances with antifungal and antibacterial activities.

COMPARISON OF MECHANICAL PROPERTIES IN 4 INDIRECT COMPOSITE RESIN (4종의 간접법용 복합 레진의 기계적 특성 비교)

  • Kim, Kil-Soo;Yoon, Tae-Ho;Song, Kwang-Yeob;Ahn, Seung-Geun
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.1
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    • pp.21-33
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    • 2007
  • Statement of problem: The esthetic component of dental care has become increasingly more important, while new tooth-colored materials are continually marketed. Various new indirect composite materials have been developed with required advantages. The most recent development in the indirect composites has been the introduction of the second-generation laboratory composite or poly-glass materials. They are processed by different laboratory techniques based on combinations of heat, pressure, vacuum and light polymerization. Although, second generation products became available in 1995, their characteristics and clinical performance have not been adequately investigated. Purpose: The aim of this study was to measure the mechanical properties of the second generation indirect resin system and compare these with an existing universal direct composite resin. Material and method: In this study four indirect composite material (Adoro LC, BelleGlass HP, Tescera, Synfony) were tested for flexural strength, wear resistance, hardness and their degree of conversion against Z250, a light cure direct composite. Results: Within the limitations of this study, the following conclusions were drawn: 1. From the abrasion wear result, Adoro showed the least volume loss while Synfony showed the greatest volume loss. Z250 and BelleGlass HP didn't show significant difference (p>0.05), but they showed significant difference with other groups (p<0.05). From the attrition wear, BelleGlass HP showed the least volume loss and it didn’t show significant difference with Tescera (p>0.05). While Synfony showed the greatest volume loss that it showed significant difference with other groups (p>0.05). 2. Mean values of flexural strength by means of three point bending test was in the order of Z250, Adoro, Belleglass HP, Tescera and Synfony. Mean elastic modulus was in the order of Z250, BelleGlass HP, Tescera, Adoro and Synfony. 3. The result of Vicker‘s microhardness value showed that significantly higher value in Z250 (p<0.05), and is in the order of BelleGlass HP, Tescera, Adoro and Synfony. 4. The degree of conversion measured by FT-IR showed significantly higher value in BelleGlass HP (p<0.05), and is in the order of Adoro, Synfony, Tescera and Z250. Conclusion: Significant differences were found in the flexural strength, wear resistance, hardness and their degree of conversion.

TREATMENT OF COMPOSITE RESIN RESTORATION WITH THE AIR ABRASIVE TECHNIQUE (Air abrasive technique을 이용한 복합레진 수복 증례)

  • Lee, Chang-Woo;Jang, Ki-Taeg;Lee, Sang-Hoon;Hahn, Se-Hyun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.24 no.4
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    • pp.763-770
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    • 1997
  • The air abrasive technique is a non-mechanical method by which teeth are treated before restoration and stains and calculi are removed from tooth surfaces using the kinetic energy of small particles. The air abrasive technique in dentistry was first introduced in the 1950's with as instrument called 'Airdent'. But, as the main restorative materials of the period were amalgam and gold, and the instrument's inability to control the flow of particles caused the particles to be spread throughout the clinics, widespread use was not possible. In the 1990's, as these techincal problems were solved and more interest in new restorative materials rose in an effort to preserve sound tooth structure, new developements took place in instruments related to the air abrasive technique. The air abrasive technique produces less pressure, vibration and heat that might cause patient discomfort and facilitates the preservation of sound tooth structure. It also reduces the need for anesthesia and is less harmful to the pulp. Other advantages include increase in dentin bonding strength of composite resin, lower possibility of saliva contamination and maintenance of a dry field. But there is not direct contact between the nozzle and the tooth, the operator cannot use his or her tactile sense and must rely solely upon visual input. Other disadvantages are: the tooth preparation depends on the operator's ability; alpha-alumina particles, after bouncing off the tooth surface, cause damage to dental mirrors; the equipment is expensive and takes up a certain amount of space in the clinic. The author conducted case report using the air abrasive technique on patient visiting the Department of Pediatric Dentistry at Seoul National University Dental Hospital and arrived at the following conclusions. 1. The tooth preparation capability of different air abrasive devices varied widely among manufacturers. 2. It was more effective in treating early caries lesions and stains compared to lesions where caries had already progressed to produce soft dentin. 3. The cold stream and noise caused by the evacuation system was a major cause of discomfort to pediatric patients. 4. As there is no direct contact with tooth surface when using the air abrasive technique for tooth preparation, considerable experience and skill is required for proper tooth preparation.

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A Study on Rehabilitation Treatment Using Radiofrequency Treatment (고주파 통증치료기를 이용한 재활 치료에 대한 연구)

  • Jo, Jae-Hyun;Lee, Sang-Yong;Lee, Geun-Yong;Yoon, Se-Jin;Cheong, Ha-Young;Lee, Sang-Sik
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.13 no.3
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    • pp.212-218
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    • 2020
  • When Radiofrequency energy is applied to the human body, the vibration width is very short. Therefore, the electrolyte burn generated when using the direct current does not occur. Ion molecules, polarized molecules, etc., vibrate more than 40,000 times per second, converting them into frictional heat to generate deep heat. The blood flow of capillaries increases 4-5 times more than at rest, increasing the supply of oxygen, nutrients, antibodies, and white blood cells. In addition, the electrochemical reaction does not occur because the vibration width and the pulsation period are very short. It is a physical factor treatment method that does not stimulate the sensory nerves and motor nerves. In this study, an isotonic exercise is performed in a young normal adult using a Radiofrequency pain treatment device. The purpose of this study is to integrate rehabilitation therapy by measuring electromyography data during isotonic exercise and confirming the effect on changes in motor neuron response. The EMG data generated when isotonic exercise of the forearm biceps muscle and the EMG data measured after the use of a Radiofrequency pain treatment device after exercise were RMS, respectively, and verified through t-test. It was confirmed that there was a significant difference in both men and women because the t-value was smaller than the significance level p (<.05).

The CH3CHO Removal Characteristics of Lightweight Aggregate Concrete with TiO2 Spreaded by Low Temperature Firing using Sol-gel Method (Sol-gel법으로 이산화티탄(TiO2)을 저온소성 도포시킨 경량골재콘크리트의 아세트알데히드(CH3CHO) 제거 특성)

  • Lee, Seung Han;Yeo, In Dong;Jung, Yong Wook;Jang, Suk Soo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.31 no.2A
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    • pp.129-136
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    • 2011
  • Recently studies on functional concrete with a photocatalytic material such as $TiO_2$ have actively been carried out in order to remove air pollutants. The absorbtion of $TiO_2$ from those studies is applied by it being directly mixed into concrete or by suspension coated on the surface. When it comes to the effectiveness, the former process is less than that of the latter compared with the $TiO_2$ use. As a result, the direct coating of $TiO_2$ on materials' surface is more used for effectiveness. The Surface spread of it needs to have a more than $400^{\circ}C$ heat treat done to stimulate the activation and adhesion of photocatalysis. Heat treat consequently leads hydration products in concrete to be dehydrated and shrunk and is the cause of cracking. The study produces $TiO_2$ used Sol-gel method which enables it to be coated with a low temperature treat, applies it to pearlite using Lightweight Aggregate Concrete fixed with a low temperature treat and evaluates the spread performance of it. In addition to this, the size of pearlite is divided into two types: One is 2.5 mm to 5.0 mm and the other is more than 5.0 mm for the benefit of finding out the removal characteristics of $CH_3CHO$ whether they are affected by pearlite size, mixing method and ratio with $TiO_2$ and elapsed time. The result of this experiment shows that although $TiO_2$ produced by Sol-gel method is treated with 120 temperature, it maintains a high spread rate on the XRF(X ray Florescence) quantitative analysis which ranks $TiO_2$ 38 percent, $SiO_2$ 29 percent and CaO 18 percent. In the size of perlite from 2.5 mm to 5.0 mm, the removal characteristic of $CH_3CHO$ from a low temperature heated Lightweight concrete appears 20 percent higher when $TiO_2$ with Sol-gel method is spreaded on the 7 percent of surface. In other words, the removal rate is 94 percent compared with the 72 percent where $TiO_2$ is mixed in 10 percent surface. In more than 5.0 mm sized perlite, the removal rate of $CH_3CHO$, when $TiO_2$ is mixed with 10 percent, is 69 percent, which is similar with that of the previous case. It suggests that the size of pearlite has little effects on the removal rate of $CH_3CHO$. In terms of Elapsed time, the removal characteristic seems apparent at the early stage, where the average removal rate for the first 10 hours takes up 84 percent compared with that of 20 hours.

Synthesis of Pt-Bi/Carbon Electrodes by Reduction Method for Direct Methanol Fuel Cell (환원법에 의한 직접 메탄올 연료전지(DMFC)용 Pt-Bi/Carbon 전극제조)

  • Kim, Kwan Sung;Kim, Min Kyung;Noh, Dong Kyun;Tak, Yongsug;Baeck, Sung-Hyeon
    • Applied Chemistry for Engineering
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    • v.22 no.5
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    • pp.479-485
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    • 2011
  • Pt-Bi/C catalysts supported on carbon black with various Pt/Bi ratios were synthesized by a reduction method. Chloroplatinic acid hydrate ($H_2PtCl_6{\cdot}xH_2O$) and bismuth (III) nitrate pentahydrate ($Bi(NO_3)_3{\cdot}5H_2O$) were used as precursors for Pt and Bi, respectively. Before loading metal on carbon, heat treatment and pretreatment of carbon black in an acidic solution was conducted to enhance the degree of dispersion. The physical property of the synthesized catalysts was investigated by X-ray diffraction and X-ray photoelectron spectroscopy. The XRD pattern of untreated Pt-Bi/C catalyst showed BiPt and $Bi_2Pt$ peaks in addition to Pt peaks. These results imply that Bi atoms were incorporated into the Pt crystal lattice by Pt-Bi alloy formation. The catalytic activity for methanol oxidation was measured using cyclic voltammetry in a mixture of 0.5 M $H_2SO_4$ and 0.5 M $CH_3OH$ aqueous solution. The addition of proper amount of Bi was found to significantly improve catalytic activity for methanol oxidation. The catalytic activity for methanol oxidation was closely related to the stability between electrode and electrolyte. In order to investigate the stability of catalysts, chronoamperometry analysis was carried out in the same solution at 0.6 V.

Mechanical Properties of Fiber-reinforced Cement Composites according to a Multi-walled Carbon Nanotube Dispersion Method (다중벽 탄소나노튜브의 분산방법에 따른 섬유보강 시멘트복합체의 역학적 특성)

  • Kim, Moon-Kyu;Kim, Gyu-Yong;Pyeon, Su-Jeong;Choi, Byung-Cheol;Lee, Yae-Chan;Nam, Jeong-Soo
    • Journal of the Korea Institute of Building Construction
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    • v.24 no.2
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    • pp.203-213
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    • 2024
  • This study delves into the mechanical properties of fiber-reinforced cement composites(FRCC) concerning the dispersion method of multi-walled carbon nanotubes(MWCNTs). MWCNTs find utility in industrial applications, particularly in magnetic sensing and crack detection, owing to their diverse properties including heat resistance and chemical stability. However, current research endeavors are increasingly directed towards leveraging the electrical properties of MWCNTs for self-sensing and smart sensor development. Notably, achieving uniform dispersion of MWCNTs poses a challenge due to variations in researchers' skills and equipment, with excessive dispersion potentially leading to deterioration in mechanical performance. To address these challenges, this study employs ultrasonic dispersion for a defined duration along with PCE surfactant, known for its efficacy in dispersion. Test specimens of FRCC are prepared and subjected to strength, drawing, and direct tensile tests to evaluate their mechanical properties. Additionally, the influence of MWCNT dispersion efficiency on the enhancement of FRCC mechanical performance is scrutinized across different dispersion methods.

Direct bonding of Si(100)/Si$_3$N$_4$∥Si (100) wafers using fast linear annealing method (선형열처리를 이용한 Si(100)/Si$_3$N$_4$∥Si (100) 기판쌍의 직접접합)

  • Lee, Young-Min;Song, Oh-Song;Lee, Sang-Hyun
    • Korean Journal of Materials Research
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    • v.11 no.5
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    • pp.427-430
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    • 2001
  • We prepared 10cm-diameter Si(100)/500 $\AA$-Si$_3$N$_4$/Si(100) wafer Pairs adopting 500 $\AA$ -thick Si$_3$N$_4$layer as insulating layer between single crystal Si wafers. Si3N, is superior to conventional SiO$_2$ in insulating. We premated a p-type(100) Si wafer and 500 $\AA$ -thick LPCVD Si$_3$N$_4$∥Si (100) wafer in a class 100 clean room. The cremated wafers are separated in two groups. One group is treated to have hydrophobic surface and the other to have hydrophilic. We employed a FLA(fast linear annealing) bonder to enhance the bond strength of cremated wafers at the scan velocity of 0.1mm/sec with varying the heat input at the range of 400~1125W. We measured bonded area using a infrared camera and bonding strength by the razor blade crack opening method. We used high resolution transmission electron microscopy(HRTEM) to probe cross sectional view of bonded wafers. The bonded area of two groups was about 75%. The bonding strength of samples which have hydrophobic surface increased with heat input up to 1577mJ/$m^2$ However, bonding strength of samples which have hydrophilic surface was above 2000mJ/$m^2$regardless of heat input. The HRTEM results showed that the hydrophilic samples have about 25 $\AA$ -thick SiO layer between Si and Si$_3$N$_4$/Si and that maybe lead to increase of bonding strength.

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Development of a Climate Change Vulnerability Index on the Health Care Sector (기후변화 건강 취약성 평가지표 개발)

  • Shin, Hosung;Lee, Suehyung
    • Journal of Environmental Policy
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    • v.13 no.1
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    • pp.69-93
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    • 2014
  • The aim of this research was to develop a climate change vulnerability index at the district level (Si, Gun, Gu) with respect to the health care sector in Korea. The climate change vulnerability index was esimated based on the four major causes of climate-related illnesses : vector, flood, heat waves, and air pollution/allergies. The vulnerability assessment framework consists of six layers, all of which are based on the IPCC vulnerability concepts (exposure, sensitivity, and adaptive capacity) and the pathway of direct and indirect impacts of climate change modulators on health. We collected proxy variables based on the conceptual framework of climate change vulnerability. Data were standardized using the min-max normalization method. We applied the analytic hierarchy process (AHP) weight and aggregated the variables using the non-compensatory multi-criteria approach. To verify the index, sensitivity analysis was conducted by using another aggregation method (geometric transformation method, which was applied to the index of multiple deprivation in the UK) and weight, calculated by the Budget Allocation method. The results showed that it would be possible to identify the vulnerable areas by applying the developed climate change vulnerability assessment index. The climate change vulnerability index could then be used as a valuable tool in setting climate change adaptation policies in the health care sector.

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