• Title/Summary/Keyword: direct determination

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Determination of Inorganic Phosphate in Paprika Hydroponic Solution using a Laboratory-made Automated Test Stand with Cobalt-based Electrodes (코발트전극과 자동시험장치를 이용한 파프리카 양액 내 무기인산 측정)

  • Kim, Hak-Jin;Son, Dong-Wook;Kwon, Soon-Goo;Roh, Mi-Young;Kang, Chang-Ik;Jung, Ho-Seop
    • Journal of Biosystems Engineering
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    • v.36 no.5
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    • pp.326-333
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    • 2011
  • The need for rapid on-site monitoring of hydroponic macronutrients has led to the use of ion-selective electrodes, because of their advantages over spectrophotometric methods, including simple methodology, direct measurement of analyte, sensitivity over a wide concentration range, and low cost. Stability and repeatability of response can be a concern when using multiple ion-selective electrodes to measure concentrations in a series of samples because accuracy might be limited by drifts in electrode potential. A computer-based measurement system could improve accuracy and precision because of both consistent control of sample preparation and easy calibration of sensors. Our goal was to investigate the applicability of a cobalt-based electrode used in conjunction with a laboratory-made automated test stand for quantitative determination of ${PO_4}^-$ in hydroponic solution. Six hydroponic solutions were prepared by diluting highly concentrated paprika hydroponicsolution to provide a concentration range of 1 to 300 ppm $PO_4$-P. A calibration curve relating electrode response to phosphate in paprika hydroponic solution titrated to pH 4 with 0.025M KHP was developed based on the Nikolskii-Eisenman equation with a coefficient of determination ($R^2$) of 0.94. The laboratory-made test stand consisting of three cobalt-based electrodes measured phosphate concentrations similar to those obtained with standard laboratory methods (a regression slope of 0.98 with $R^2$ = 0.80). However, the y intercept was relatively high, 30 ppm, probably due to the relatively large amount of variation present among multiple measurements of the same sample. Further studies on the high variation in EMFs obtained with cobalt electrodes during replicate measurements were required for P estimations comparable to those obtained with standard laboratory instruments.

Determination of Microbial Community as an Indicator of Kimchi Fermentation (김치발효의 지표로서 미생물군집의 측정)

  • Han, Hong-Ui;Lim, Chong-Rak;Park, Hyun-Kun
    • Korean Journal of Food Science and Technology
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    • v.22 no.1
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    • pp.26-32
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    • 1990
  • Attempts were made to define the characteristics of microbial community as an indicator of Kimchi fermentation. Determination of communities was carried out by simple Gram-stain, followed by direct microcopic counts. In room-temperature $(15^{\circ}C)$ fermentation, microbial succession was occurred in the order of communities of Gram-positive bacteria, yeasts and Gram-negative bacteria. It was characteristic that Gram-positive bacterial community was developed during the production of lactic acid, yeasts community was developed to cause rancidity, and Gram-negative bacterial community was relevant to maceration (or softening) as well as rancidity. The fluctuation of apparent Gram-negative reaction group might be used as a criterion of death or aging of Gram-positive bacterial populations. In low-temperature fermentation $(5^{\circ}C)$, however, it was found that yeasts and Gram-negative bacterial communities did not developed but only Gram-positive bacterial community did. It follows from these results mentioned above that maturity of Kimchi depends on the development of Cram-positive bacterial community. Thus, the size and occurrence of microbial community are avaiable for an indicator of Kimchi fermentation, and also determination of community could be a useful method to predict the maturity.

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Seam-line Determination in Image Mosaicking using Adaptive Cost Transform and Dynamic Programming (동적계획법과 적응 비용 변환을 이용한 영상 모자이크의 seam-line 결정)

  • Chon, Jae-Choon;Suh, Yong-Cheol;Kim, Hyong-Suk
    • Journal of the Korean Association of Geographic Information Studies
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    • v.7 no.2
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    • pp.16-28
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    • 2004
  • A seam-line determination algorithm is proposed to determine image border-line in mosaicing using the transformation of gray value differences and dynamic programming. Since visually good border-line is the one along which pixel differences are as small as possible, it can be determined in association with an optimal path finding algorithm. A well-known effective optimal path finding algorithm is the Dynamic Programming (DP). Direct application of the dynamic programming to the seam-line determination causes the distance effect, in which seam-line is affected by its length as well as the gray value difference. In this paper, an adaptive cost transform algorithm with which the distance effect is suppressed is proposed in order to utilize the dynamic programming on the transformed pixel difference space. Also, a figure of merit which is the summation of fixed number of the biggest pixel difference on the seam-line (SFBPD) is suggested as an evaluation measure of seamlines. The performance of the proposed algorithm has been tested in both quantitively and visually on various kinds of images.

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Characterization of Surfaces by Contact Angle Goniometry - I. Contact Angle Measurement by Laser Beam Projection- (접촉각측정에 의한 표면의 특성연구 - I. 레이저광선 투영에 의한 접촉각의 측정방법-)

  • Park Chung Hee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.15 no.1
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    • pp.70-75
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    • 1991
  • Contact angle measuring device was developed in this laboratory using laser beam projec-tion. The new method allows for rapid and direct determination of stationary, advancing, and receding contact angles on both planar and nonplanar solid surfaces, including fibers with very small diameters. A narrow laser beam impinges on an edge of an interface of liquid and solid. This makes two projected laser beam lines upon and radiating from the center of a protractor scale on a tangent screen. Contact angle is measured by determining the difference in angle on the protractor scale between the two projected laser beam lines. Contact angles measured on Perspex-CQ using this instrument were in agreement with the literature. it was shown that this instrument provides a novel method for the facile and accurate measurement of contact angles.

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Determination and Applications of U and K$_{op}$ for Crack Closure Evaluation under Mixed-mode loading (혼합모드 하중 하에서 균열닫힘 평가에 대한 K$_{op}$와 U의 결정과 적용)

  • Song Samhong;Seo Kijeong;Lee Jeongmoo
    • Transactions of the Korean Society of Automotive Engineers
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    • v.13 no.3
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    • pp.178-185
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    • 2005
  • Crack tip displacement is originated by tensile stress component, s and shear stress component, t on pure Mode I and pure Mode II. The crack tip displacement(CTD) depends on combined types of different two stress components under mixed-mode loading conditions (MMLC). Thus, the analysis of crack tip displacement must be CTD vector, dv which is composition of ds and dt under MMLC. In this paper, various effects of MMLC on the crack closure are studied experimentally. The crack closure magnitude is calculated from the information of crack tip displacement under MMLC. This information has been obtained from the high resolution optical microscope in direct observations of crack displacement behavior at the crack tip. Observed crack tip displacement is analyzed by using CTD vector to determine crack opening load. The various effects of MMLC on the crack closure are explained using crack opening ratio with crack length and mode mixture. The effective stress intensity factor considering crack closure is also discussed.

Simultaneous Determination of Both Surface Profiles of a Bifocal Lens Using Dual-Wavelength Transmission Deflectometry With Liquid (액체와 2 파장 투과형 편향법을 이용한 다초점 렌즈 양면 프로파일 동시측정)

  • Shin, Sanghoon;Yu, Younghun
    • Korean Journal of Optics and Photonics
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    • v.26 no.3
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    • pp.147-154
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    • 2015
  • We propose a method for simultaneously measuring the front and back surface profiles of transparent optical components. The proposed method combines dual-wavelength transmission deflectometry with liquids to record distorted phases at different wavelengths, and then numerically reconstructs the three-dimensional phase information to image the front and back surfaces of the lens. We propose a theoretical model to determine the surface information, and a bifocal lens is experimentally investigated. Unlike conventional transmission deflectometry, our proposed method supports direct observation of the front and back surface profiles of the optical elements.

A basic study on Visual judgment method for the Dent of Lightweight wall surface (경량벽체 표면의 패임에 대한 시각적 판단방법에 관한 기초적 연구)

  • Kim, Jin-Sik;Shin, Yun-Ho;Choi, Soo-Kyung
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2015.11a
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    • pp.36-37
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    • 2015
  • Recently, there has been a rapid increase in demand for lightweight walls for their use as interior partitions, as types of structure have gradually changed from shear wall structures to column structures or flat plate column wall systems. The lack of resisting force in lightweight walls is found by measuring the depth of dents in impact resistance tests, but it is not a direct factor of impact resistance. However, in the user's position, dents of over a certain size are clearly a factor that visually reminds the need for repair. In this study, we reviewed relative methods of assessment of the need for repair based on the visual means of determination (sensory test) on the dents on lightweight walls. Dents were found to stand out starting from depths of about 4mm, and the depth of roughly 5mm was found to be the criterion for determining the necessity of repair for men, while it was 4mm for women.

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Ezrin-radixin-moesin proteins are regulated by Akt-GSK3β signaling in the rat nucleus accumbens core

  • Kim, Wha Young;Cai, Wen Ting;Jang, Ju Kyong;Kim, Jeong-Hoon
    • The Korean Journal of Physiology and Pharmacology
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    • v.24 no.1
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    • pp.121-126
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    • 2020
  • The ezrin-radixin-moesin (ERM) proteins are a family of membrane-associated proteins known to play roles in cell-shape determination as well as in signaling pathways. We have previously shown that amphetamine decreases phosphorylation levels of these proteins in the nucleus accumbens (NAcc), an important neuronal substrate mediating rewarding effects of drugs of abuse. In the present study, we further examined what molecular pathways may be involved in this process. By direct microinjection of LY294002, a PI3 kinase inhibitor, or of S9 peptide, a proposed GSK3β activator, into the NAcc core, we found that phosphorylation levels of ERM as well as of GSK3β in this site are simultaneously decreased. These results indicate that ERM proteins are under the regulation of Akt-GSK3β signaling pathway in the NAcc core. The present findings have a significant implication to a novel signal pathway possibly leading to structural plasticity in relation with drug addiction.

Estimation of BOD in wastewater treatment plant by using different ANN algorithms

  • BAKI, Osman Tugrul;ARAS, Egemen
    • Membrane and Water Treatment
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    • v.9 no.6
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    • pp.455-462
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    • 2018
  • The measurement and monitoring of the biochemical oxygen demand (BOD) play an important role in the planning and operation of wastewater treatment plants. The most basic method for determining biochemical oxygen demand is direct measurement. However, this method is both expensive and takes a long time. A five-day period is required to determine the biochemical oxygen demand. This study has been carried out in a wastewater treatment plant in Turkey (Hurma WWTP) in order to estimate the biochemical oxygen demand a shorter time and with a lower cost. Estimation was performed using artificial neural network (ANN) method. There are three different methods in the training of artificial neural networks, respectively, multi-layered (ML-ANN), teaching learning based algorithm (TLBO-ANN) and artificial bee colony algorithm (ABC-ANN). The input flow (Q), wastewater temperature (t), pH, chemical oxygen demand (COD), suspended sediment (SS), total phosphorus (tP), total nitrogen (tN), and electrical conductivity of wastewater (EC) are used as the input parameters to estimate the BOD. The root mean squared error (RMSE) and the mean absolute error (MAE) values were used in evaluating performance criteria for each model. As a result of the general evaluation, the ML-ANN method provided the best estimation results both training and test series with 0.8924 and 0.8442 determination coefficient, respectively.

A New Cone Shaped Asymmetrically Substituted Calix[4]arene as an ExcellentIonophore in Construction of Ag(I) ion-Selective Membrane Electrode

  • Ganjali, Mohammad Reza;Babaei, Leila Hajiagha;Taghvaei-Ganjali, Saeed;Modjallal, Atoosa;Sahmsipur, Mojtaba;Hosseini, Morteza;Javanbakht, Mehran
    • Bulletin of the Korean Chemical Society
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    • v.25 no.2
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    • pp.177-181
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    • 2004
  • A PVC membrane electrode for silver ion based on a new cone shaped calix[4]arene (CASCA) as membrane carrier was prepared. The electrode exhibits a Nernstian response for $Ag^+$ over a wide concentration range ($1.0{\times}10^{-1}-8.0{\times}10^{-6}$M) with a slope of 58.2 {\pm}$ 0.5 mV per decade. The limit of detection of the sensor is $5.0{\times}10^{-6}$M. The sensor has a very fast response time (~5 s) in the concentration range of ${\leq}=1.0{\times}10^{-3}$ M, and a useful working pH range of 4.0-9.5. The proposed sensor displays excellent discriminating ability toward $Ag^+$ ion with respect to common alkali, alkaline earth, transition and heavy metal ions. It was used as an indicator electrode in potentiometric titration of $Ag^+$ with EDTA and in direct determination of silver ion in wastewater of silver electroplating.