• Title/Summary/Keyword: digalactosyldiglyceride

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Fractionation and Quantitative Analysis of Lipid Components in Korean Yam (Dioscorea) Tubers (한국산 마의 지질 성분의 분리 및 분석)

  • Chung, Hae-Young
    • Applied Biological Chemistry
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    • v.37 no.6
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    • pp.509-515
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    • 1994
  • Using the lipids extracted from Korean yam(Dioscorea) tubers, D. batatas, D. aimadoimo and D. japonica, fractionation and identification of lipid components and their fatty acid compositions were analysed. Lipid contents determined by Folch's method in D. batatas, D. aimadoimo and D. japonica were 11.0 mg/g, 11.4 mg/g and 6.6 mg/g, respectively. Lipids extracted were fractionated into neutral lipid, glycolipid and phospholipid by silicic acid column chromatography. The content of neutral lipid was over about 60% in lipid. Neutral lipid was composed of sterol ester, triglyceride, 1,3-diglyceride, 1,2-diglyceride and monoglyceride. Main constituents of glycolipid were acylsterylglycoside, monogalactosyldiglyceride, sterylglycoside, digalactosyldiglyceride and sulfolipid, and phospholipid contained phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. The fatty acids of the total lipid and its three lipid fractions were analyzed by GC. The major fatty acids were palmitic and linoleic acids. Content of the saturated fatty acids was less than that of the unsaturated fatty acids.

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Changes of Lipids in Chestnut during Storage (밤의 저장중 지방질 조성의 변화)

  • Nha, Young-Ah;Yang, Cha-Bum
    • Korean Journal of Food Science and Technology
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    • v.29 no.3
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    • pp.437-445
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    • 1997
  • Studies were carried out to investigate the changes of lipids in chestnut (Castanea crenata Sieb. et Zucc) during storage at $20^{\circ}C$ for 9 weeks and $1^{\circ}C$ for 15 weeks. Lipid composition of fresh chestnuts were 43.4% of nonpolar lipid (NPL), 26.5% of glycolipid (GL) and 30.1% of phospholipid (PL) in total lipid (TL). Nonpolar lipid was composed of triglyceride (TG), 1,2-diglyceride (1,2-DG), 1,3-diglyceride (1,3-DG), sterolester (SE), sterol (S) and free fatty acid (FFA). Main constituents of glycolipid were digalactosyldiglyceride (DGDG), monogalactosyldiglyceride (MGDG), sterylglycoside (SG), and acylsteryglycoside (ASG). Main constituents of phospholipid were phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI). The content of nonpolar lipid was decreased after 5 weeks of storage, and glycolipid content was increased during 7 weeks and then decreased. Phospholipid was the most increased lipid during storage at $20^{\circ}C$ and $1^{\circ}C$. The contents of TG, SE, S, DGDG and MGDG were increased during storage at $20^{\circ}C$ and $1^{\circ}C$. The major fatty acids were linoleic acid, oleic acid, palmitic acid and linolenic acid in TL, NPL, GL, PL, TG, DG, ASG, PC and PE. The content of palmitic acid was relatively higher in the PL. Linoleic acid among them was increased during storage at $20^{\circ}C$ and $1^{\circ}C$, while oleic acid was decreased.

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The Effects of Metal Compounds on the Biosynthesis of the Galactolipid and Composition of Fatty Acids in Escherichia coli and Bacillus subtilis (Escherichia coli와 Bacillus subtilis의 당지질 생합성과 지방산 조성에 미치는 금속산화물의 효과)

  • Lee, So Yeon;Yoon, Hyo Sook;Choi, Won Chang;Lee, Chong Sam
    • Journal of Environmental Health Sciences
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    • v.23 no.2
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    • pp.12-23
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    • 1997
  • The biosynthesis of galactolipid, galactose and the fatty acid composition in E. cdi and B. subtills treated with potassium dichromate(PD, 500 ppm, 500 ppm), potassium chromate(PC, 500 ppm, 500 ppm), cobalt chloride(CC, 100 ppm, 10 ppm) and methylmercuric chloride(MC, 100 ppm, 10 ppm) during the culture were analyzed to compare with the control. The growth rate of cells, the contents of monogalactosyldiglyceride(MGDG), digalactosyldiglyceride(DGDG) and total lipid in the metal compound treatments were lower as compared with the control. And too, the contents of galactose utilized for the biosynthesis of galactolipids in these strains in the various metal compounds treatments were inhibited. The fatty acids used for the MGDG and DGDG formation in E. coli and B. subtills treated with each metal compounds during the culture were showed to the variant compositional change.

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