• 한국발생생물학회지:발생과생식
• /
• 제15권4호
• /
• pp.331-338
• /
• 2011

MicroRNAs (miRNAs) function as a key regulator of diverse cellular functions. To find out novel miRNAs that promote the differentiation of mouse embryonic stem cells (mESCs), we compared the miRNAs expression profiles of mESCs under self-renewal vs. differentiation states. We noticed that miR-222 was highly expressed during the differentiation of mESCs. Quantitative RT-PCR analysis revealed that expression of miR-222 was up-regulated during the embryonic bodies formation and retinoic acid -dependent differentiation. When miR-222 was suppressed by antogomiR-222, the differentiation of mESCs was delayed compared to control. Self-renewal marker expression or cell proliferation was not affected but the expression of lineage specific marker was suppressed by the treatment of miR-222 inhibitor during the differentiation of mESCs. Taken together, these results suggest that miR-222 functions to promote the differentiation of mESCs by regulating expression of differentiation related genes.

• Reproductive and Developmental Biology
• /
• 제30권2호
• /
• pp.143-156
• /
• 2006

Major characteristics of embryonic stem cells (ESCs) are sustaining of sternness and pluripotency by self-renewal. In this report, transcriptional profiles of the molecules in the developmentally important signaling pathways including Wnt, BMP4, $TGF-\beta$, RTK, Hh, Notch, and JAK/STAT signaling pathways were investigated to understand the self-renewal of mouse ESCs (mESCs), J1 line, and compared with the NIH3T3 cell line and mouse embryonic fibroblast (MEF) cells as controls. In the Wnt signaling pathway, the expression of Wnt3 was seen widely in mESCs, suggesting that the ligand may be an important regulator for self-renewal in mESCs. In the Hh signaling pathway, the expression of Gli and N-myc were observed extensively in mESCs, whereas the expression levels of in a Shh was low, suggesting that intracellular molecules may be essential for the self-renewal of mESCs. IGF-I, IGF-II, IGF-IR and IGF-IIR of RTK signaling showed a lower expression in mESCs, these molecules related to embryo development may be restrained in mESCs. The expression levels of the Delta and HESS in Notch signaling were enriched in mESCs. The expression of the molecules related to BMP and JAK-STAT signaling pathways were similar or at a slightly lower level in mESCs compared to those in MEF and NIH3T3 cells. It is suggested that the observed differences in gene expression profiles among the signaling pathways may contribute to the self-renewal and differentiation of mESCs in a signaling-specific manner.

• 한국생활과학회지
• /
• 제17권5호
• /
• pp.873-889
• /
• 2008

The purpose of this study was to investigate the effects of family-of-origin experiences(parents' marital adjustment and affectionate relationship with parents) and self-differentiation on the nuclear family relationships(marital adjustment of married men women, and affectionate relationship with child). Data were collected using self-administered questionnaire method with 159 married men and 193 married women. The results showed that first, married men and women perceived the level of family-of-origin experiences lower than that of nuclear family relationships, and there were significant differences in the level of marital adjustment across sex, age, marital status, and income. Second, there was significant positive correlation between family-of-origin experiences and self-differentiation, between family-of-origin experiences and the nuclear family relationships, and between self-differentiation and the nuclear family relationships. Third, multiple regression analyses indicated that parents' marital adjustment and emotional cutoff were the powerful predictors of marital adjustment of married men women. Finally, women's affectionate relationship with child was predicted by such variables as affectionate relationship with parents, fusion with others, and emotional fusion, whereas men's affectionate relationship with child was predicted by such variables as affectionate relationship with parents, emotional reactivity, and I-position.

• 한국가족관계학회지
• /
• 제21권1호
• /
• pp.99-121
• /
• 2016

Objectives: The major purpose of this study was to analyze the relationships of parent-child relationship and self-differentiation with interpersonal competence among college students. Specifically, this study also attempted to find the differences of three major variables according to participant's general characteristics, to investigate the correlations among variables and to analyze the effects of related variables on interpersonal competence. Method: The participants were 388 college students in Jeollabuk-do province. The data were collected by self-administered questionnaire method. Results: First, there were significant differences in the level of interpersonal competence according to gender and the monthly family income. Second, correlational analyses results indicated that college students' interpersonal competence was positively related with parent-child relationship and self-differentiation, and the latter two variables were also positively correlated. Finally, the results of multiple regression analyses reported that interpersonal competence was influenced by intimacy with mother, I-position, and emotional cutoff, showing that I-position was the most influential variable. In addition, I-position was also found to be the powerful variable affecting all sub-factors of interpersonal competence. Conclusions: This study suggests the importance of providing education and/or counseling services focusing on strengthening the relationship with their parents and on increasing self-differentiation level for college students' greater interpersonal competence, which are considered as critical indices of their mental health and psychological well-being.

• 한국가정과학회지
• /
• 제3권2호
• /
• pp.13-26
• /
• 2000

The purpose of present study were to find the general trends of mate selecting factors and. marital satisfaction. concentrated on married women, to examine the difference among mate selection factors and marital satisfaction according to socio-demographic variables and Psychological variables and to analyze the effects of these variables influencing marital satisfaction. The subjects were 276 wives, living in Kwangju that having passed under S years after marriage without divorce experience. The major findings were as follows . 1. In mate selection, factor of high-degree was personality. view of value. personal relations, achievement, emotional mature. self-differentiation. degree of affection's expression. sense of humor, charms and condition of health Respondents'marital satisfaction score showed 91.75 and this score was higher than median score(62.5) 2. The external factor of mate selection showed significant difference according to degree of education. career. order. and sex-role attitude. The internal factor of mate selection showed significant difference according to degree of education, career, order, self-differentiation, self-esteem, and sex-role attitude 3. As correlating mate selectional factors to marital satisfaction, the significance appears in the mate's personality. view of value, emotional mature. personal relations. self-differentiation, condition of health. achievement. charm, sense of humor and degree of affection's expression. 4. Married women's marital satisfaction was influenced by self-esteem, personality and child's number that were explained about 38% by these variables. In conclusion, to happy marital life must be loved her own self. and above all considered internal factors like personality than external factors in mate selection.

• 대한간호학회지
• /
• 제45권1호
• /
• pp.64-75
• /
• 2015

Purpose: This study was designed to construct and test structural equation modeling on healthy menopausal transition in middle-aged women in order to identify variables affecting healthy menopausal transition. Methods: Participants, 276 women, 45 to 60 years of age, with menopausal symptom score higher than 5 on the Korean version of Menopause Rating Scale, were recruited in three cities and one county of Gyeongnam Province. Research data were collected via questionnaires and analysed using SPSS version 18.0 and AMOS version 20.0. Results: After confirmatory factor analysis, one of the observed variables was excluded due to relatively low factor loading. The model fit indices for the hypothetical model were suitable for the recommended level: GFI=.93, CFI=.92, RMSEA=.05. Self-efficacy, self-differentiation, and menopausal symptoms explained 67.7% of variance in menopausal transition, and self-differentiation was the most influential factor for menopausal transition. Self efficacy and menopausal symptoms explained 9.6% of variance in menopausal management, although "menopausal symptoms" was not significant. Conclusion: These results suggest that nursing interventions to improve self-differentiation, self efficacy, menopausal management and decrease menopausal symptoms are critical for healthy menopausal transition in middle-aged women. Continued development of a variety of community-based nursing interventions to facilitate healthy menopausal transition is suggested.

• Molecules and Cells
• /
• 제38권9호
• /
• pp.806-813
• /
• 2015

Gaucher disease (GD) is an autosomal recessive lysosomal storage disorder caused by mutations in the glucocerebrosidase gene (GBA), which encodes the lysosomal enzyme glucosylceramidase (GCase). Deficiency in GCase leads to characteristic visceral pathology and lethal neurological manifestations in some patients. Investigations into neurogenesis have suggested that neurodegenerative disorders, such as GD, could be overcome or at least ameliorated by the generation of new neurons. Bone marrowderived mesenchymal stem cells (BM-MSCs) are potential candidates for use in the treatment of neurodegenerative disorders because of their ability to promote neurogenesis. Our objective was to examine the mechanism of neurogenesis by BM-MSCs in GD. We found that neural stem cells (NSCs) derived from a neuronopathic GD model exhibited decreased ability for self-renewal and neuronal differentiation. Co-culture of GBA-deficient NSCs with BM-MSCs resulted in an enhanced capacity for self-renewal, and an increased ability for differentiation into neurons or oligodendrocytes. Enhanced proliferation and neuronal differentiation of GBA-deficient NSCs was associated with elevated release of macrophage colony-stimulating factor (M-CSF) from BM-MSCs. Our findings suggest that soluble M-CSF derived from BM-MSCs can modulate GBA-deficient NSCs, resulting in their improved proliferation and neuronal differentiation.

• 한국가족관계학회지
• /
• 제23권3호
• /
• pp.77-104
• /
• 2018

Objectives: This study aimed to examine the general tendencies of women's divorce intention and to analyze the related variables that affect women's divorce intention. It would help explore the conditions for prevention or intervention of divorce. Methods: For this purpose, the structured survey was conducted by 521 married women aged between 20 and 70 living in Jeollanam-do and Gwangju. The data was analysed by SPSS 21.0 program. Results: First, the average score of divorce intention was 2.04, which was lower than the middle. The average score of self-differentiation, marital communication efficiency, reasonable coping, and social support were a bit higher than the median score. Second, the differences in the divorce intention according to the academic background and household income were statistically significant. Third, marital communication efficiency had the biggest effect on divorce intention and it was followed by avoidance-oriented behavior, reasonable coping, support from the local community, self-differentiation, and household income. Conclusion: It is very important to understand the various factors related to divorce intention especially in individual, marital, social aspects. In addition, it is actually required to develop the couple programs about coping strategies, effective couple communication and problem-solving methods. It also have to imply the requirements considering psychological, social aspects such as self-differentiation and social support.

• 한국산학기술학회논문지
• /
• 제19권9호
• /
• pp.225-233
• /
• 2018

본 연구의 목적은 간호대학생의 대인관계능력, 자아분화, 대학생활적응 수준을 알아보고, 간호대학생들의 대학생활적응에 영향을 주는 요인을 파악하는 것이다. 연구대상자는 D시에 소재하는 2개 대학 간호학과 학생 185명이었다. 자료수집을 위한 설문조사는 2016년 11월 15일부터 12월 5일까지 실시하였고, SPSS 21을 사용하여 빈도와 백분율, 평균과 표준편차, t-test, one-way ANOVA, Pearson's correlation, Multiple regression으로 분석하였다. 연구결과, 간호대학생들의 대학생활적응정도는 5점 만점에 3.30점, 대인관계능력은 5점 만점에 3.73점, 자아분화수준은 5점 만점에 3.07점 이었다. 일반적 특성에 따른 대학생활적응의 차이는 학과지원동기, 전공만족도, 친구 수에 유의한 차이가 있었으며, 대인관계능력은 거주형태, 학과지원동기, 전공만족도, 친구의 수에 따라 차이가 있었고, 자아분화는 전공만족도에 따라 유의한 차이가 있었다. 대학생활적응은 대인관계능력(r=.614, p<.001), 자아분화(r=.264, p<.001)와 유의한 순 상관관계에 있었으며, 간호대학생의 대학생활적응에 영향을 미치는 요인은 대인관계능력, 자아분화(F=58.171, p=.000)임을 확인하였으며 이 변인들의 대학생활적응에 대한 설명력은 39%이었다. 본 연구결과에 의하면, 간호대학생의 대학생활적응을 위해서는 대인관계능력과 자아분화 수준을 향상시킬 수 있는 교과내외의 다양한 프로그램개발과 적용이 필요함을 시사한다.

• Reproductive and Developmental Biology
• /
• 제29권2호
• /
• pp.93-99
• /
• 2005

Human embryonic stem cells (hESCs) derived from the inner cell mass of blastocysts have the ability to renew themselves and to differentiate into cell types of all lineage. The present study was carried out to investigate whether the Wnt signaling pathway is related to maintaining self-renewal of hESCs. Glycogen Synthase Kinase 3 (GSK-3) inhibitor, BIO ((2'Z,3'E)-6-Bromoindirubin-3'-oxime) was treated to Miz-hES1 line for activation of Wnt signaling pathway. BIO-nontreated hESCs (control) and BID-treated hESCs were cultured for 5 days in the modified feeder-free system. During the culture of hESCs, differences were observed in the colony morphology between 2 groups. Controls were spread outwards whereas BIO-nontreated hESCs were clumped in the center and the differentiated cells were spreading outwards in the edges. The results of stem cell specific marker staining indicated that control were differentiated in large part whereas BIO-treated hESCs maintain self-renewal in the center of the colony. The results of lineage marker staining suggested that outer cells of the hESC colony were differentiated to the neuronal progenitor cells in both control and BIO-treated hESC. These results indicate that Wnt signaling is related to self-renewal in hESCs. In addition, control group showed higher composition of apoptotic cells $(23.76\%)$ than the BID-treated group $(5.59\%)$. These results indicate that BIO is effective on antapoptosis of hESCs.