• The Journal of the Society of Korean Medicine Diagnostics
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• v.19 no.3
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• pp.151-158
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• 2015

Objectives Syndrome differentiation and treatment (辨證論治) is one of the core theories in Korean medicine and syndrome differentiation (辨證) constitutes a branch of disease diagnosis in Korean medicine. Yoon Gil-Young, one of the modern outstanding scholar of basic medical science in Korean medicine, wrote on basic theories of Korean medicine such as physiology, pathology, formula science, etc. Hereby we will analyze and discuss his works to understand his recognition of historical changes in the syndrome differentiation. Methods We conducted researches into the two works of Yoon Gil-Young's, which are "The Clinical Formula Science of Eastern Medicine (東醫臨床方劑學)" and "The theory of Four-Constitution Medicine (四象體質醫學論)". From Yoon's academic standpoint which connects the basic medical science with the clinical medicine, we analyzed his opinion about syndrome differentiation and its historical changes. Results According to Yoon's research work on syndrome differentiation and its historical changes, the development of syndrome differentiation, which goes in harmony with the history of Korean medicine, has its deep root in " Huangdi's Internal Classic (黃帝內經)" and "Treatise on Cold Damage and Miscellaneous Diseases (傷寒雜病論)". And through "Treatise on the Spleen and Stomach (脾胃論)" and the articles of warm disease (溫病論), the theory of syndrome differentiation became extended to the whole clinical diagnostic field in Korean medicine, finally including the achievements in "Treasured Mirror of Eastern Medicine (東醫寶鑑)", "Longevity and Life Preservation in Eastern Medicine (東醫壽世保元)". Conclusions Yoon Gil-Young recognized that the system of syndrome differentiation was developed in accordance with the theories from the "Treatise on Cold Damage and Miscellaneous Diseases", then the "Treatise on the Spleen and Stomach" and the articles of warm disease. The four-constitution medicine in Korea and Koho school in Japan which lays emphasis on abdominal signs also contributed to its development. Syndrome differentiation can be categorized basically into three states of intrinsic cold (本寒), intrinsic deficiency (本虛), intrinsic heat (本熱) according to the deficiency and excess in human body metabolism.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.79-79
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• 2003

Embryonic stem cells are capable of differentiating into a variety of cell lineages. However, the ultimate results of differentiation in vitro greatly depend on the duration of treatment and kinds of differentiating inducers added. In order to investigate the efficiencies of various differentiation inducers and the methods of treatment, we examined differentiation patterns of human embryonic stem cell (hESC, MB03) according to several different protocols. Exp. I) Upon differentiation using retinoic acid and ascorbic acid (RA/AA), embryoid bodies (EB, for 4days) derived from hESC was exposed to Rh (10$^{-6}$ M) and AA (50 mM) for 4 days, and were allowed to differentiate in N2 medium for 7, 14, 21, or 28 days. Exp. II) When bFGF was used, neuronal precursor cells were selected for 8 days in N2 medium after EB formation. After selection, cells were expanded at the presence of bFGF (20 ng/ml) for another 6 days followed by a final differentiation in N2 medium for 7, 14, 21 or 28 days. Exp. III) In addition, to examine the effects of neurotrophic factors in the production of mature neurons, groups of cells were exposed to either BDNF (5 ng/ml) or TGF-$\alpha$(10 ng/ml) during the 28 days of final differentiation. Differentiation patterns of RA/AA or bFGF treated groups were very similar; approximately 82% and 83% of the cells, respectively, were positive for anti-NF200 antibody, while it was about 10% and 11%, respectively, for anti-NF160 antibody in 28 days in N2 medium. Alsor, cells expressing TH were as low as 5%, while the cells doubled when matured at the presence of either BDNF or TGF-$\alpha$. Cells immunoreactive to anti-GAD antibody were approximately 20%. These results suggest that a maturation step rather than differentiation induction step, which is formation of EB, effects more decisively to the ultimate differentiation pattern.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.20 no.1
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• pp.15-26
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• 2016

Objectives Syndrome differentiation and treatment (辨證論治) was one of the core theories in Korean medicine and syndrome differentiation (辨證) constitutes a branch of disease diagnosis in Korean medicine. Yoon Gil-Young, one of the modern outstanding scholar of basic medical science in Korean medicine, wrote on basic theories of Korean medicine such as physiology, pathology, formula science, etc. Hereby we will analyze and discuss his works to understand his recognition of historical changes in the syndrome differentiation. Methods We conducted researches into the two works of Yoon Gil-Young's, which are "The Clinical Formula Science of Eastern Medicine (東醫臨床方劑學)" and "The theory of Four-Constitution Medicine (四象體質醫學論)". From Yoon's academic standpoint which connects the basic medical science with the clinical medicine, we analyzed his opinion about the system in the Theory of 'Syndrome Differentiation'. Results According to Yoon's research work on the Theory of 'Syndrome Differentiation', the system of syndrome differentiation, which had its deep root in the theory of Yin and Yang (陰陽) & the theory of abbreviation of the five circuit phases (五運) and the six atomspheric influences (六氣) of the "Huangdi's Internal Classic (黃帝內經)". Conclusions Yoon Gil-Young's theory of differentiation of syndromes and treatment is widespread so much that he studied on the learning field of Traditional Korean Mediciine and ingenious as well. He explain on the main principles of differentiation of syndromes based on "Huang Di Nei Jing" and the system of differentiation of syndromes is composed of Traditional Korean Medical Physiology.

• Journal of Families and Better Life
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• v.28 no.6
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• pp.183-194
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• 2010

This study investigates the relationship between a mother's self-differentiation and the rearing reviews focused on the mediating effect for the early adjustment of 1 to 2 years old at a nursery. First, the results based on the difference between the child-rearing attitudes of the level of self-differentiation show significance in the affectional-autonomic attitude in the top quarter of the total level of self-differentiation. Second, the level of the self-differentiation depends on the early adjustment results from the significant differences in the sub-elements; except for the emotional fusion that results in the higher the level of mother's self-differentiation that leads to higher early adjustment. Third, the impact of affectional-autonomic and affectional-controlling attitude reflected the positive influence of the entire early adjustment based on the effect of the child-rearing attitude. Finally, the results on whether the mothers' parenting attitudes influences the self-differentiation and the transition of the infant would mediate the early adjustment-relationship as shown in the mediating effect of parenting attitudes. Each type of mediation effect showed an affectional-autonomic and affectional-controlling attitude. In this study, the mother's self-differentiation and the infant nursery process shows the early adjustment associated with variables, the mother's level of self-differentiation, and the parenting attitude that effects the early adjustment that examined the mediation effect in the relations of self-differentiation and the early adjustment of child-rearing attitudes.

• Korean Journal of Child Studies
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• v.28 no.6
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• pp.119-133
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• 2007

The subjects of this study were composed of 620 $5^{th}$ and $6^{th}$ grade children in Pusan City and their parents. Both parents completed the Differentiation of Self Inventory(DSI : Skowron & Friedlander, 1998). Children completed the DSI, the Personal Authority in the Family System Questionnaire (Bray et al., 1984), and the Youth Self-Report (Archenbach, 1991). These measures were translated into Korean and adapted for children and parents; their validity was established through a preliminary survey of content validity and reliability. Results showed no meaningful correlation between parent's self-differentiation and children's self-differentiation or between parents' self-differentiation total and children's level of problem behaviors. However, Intergenerational Family Relationship and Children's Self-Differentiation influenced children's internalizing and externalizing of problem behavior.

• Korean Journal of Human Ecology
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• v.16 no.2
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• pp.259-272
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• 2007

The purpose of this study was to investigate the effect of couples' self-differentiation by marital satisfaction according to couple's gender. The participants of the present study were 728 couples who lived in Chonbuk province. The data were collected by self-administered questionnaire method. Self-differentiation scale was consisted of such factors as emotional reactivity, I-position, emotional cut-off and fusion with others. The results of this research were as follows: First, there was significant difference in the level of total self-differentiation between husbands and wives. Second, there was significant difference in the levels of marital conflict and of marital satisfaction to levels of self differentiation. Third, self differentiation in both husbands and wives had an direct effect on marital satisfaction and stability through marital conflict. Fourth, marital conflict in both husbands and wives had a direct effect on marital satisfaction.

• Biomedical Science Letters
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• v.17 no.3
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• pp.267-271
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• 2011

P19 cells are pluripotent embryonal carcinoma cells and can be differentiated into neuronal cell type by treatment with retinoic acid (RA) and aggregation culture. Cannabinoids are the active components of Cannabis sativa and they have diverse pharmacologic activities, such as pain control, anti-inflammatory effects, neuro-protection effects and tumor regression. Cannabinoids also involved in neuronal proliferation, migration, differentiation and survival in developing brain. Here, we studied the role of cannabinoids on neuronal differentiation of P19 cells. Treatment with cannabinoids increased the neuronal differentiation induced by RA and also promoted transcriptional activity of neurogenin 1, key transcription factor for neuronal differentiation of P19 cells. These results suggest that the cannabinoids can accelerate neuronal differentiation of P19 cells.

• Korean Journal of Child Studies
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• v.34 no.5
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• pp.115-126
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• 2013

The purpose of this study was to investigate the relationship between self-differentiation and covert narcissism with organization citizenship behavior on the part of teachers in childcare centers. The subjects consisted of 522 teachers who worked in childcare centers in Daegu and Gyeongbuk Province. The data collected were analyzed by means of Pearson's correlation, and hierarchical regression, using SPSS Win 19.0. The results were as follows. (1) Self-differentiation was positively associated with organization citizenship behavior. However when covert narcissism was operating as control, self-differentiation was not associated with organization citizenship behavior. (2) Covert narcissism was negatively associated with organization citizenship behavior. (3) Between both covert narcissism and self-differentiation, only covert narcissism was associated with organization citizenship behavior. (4) There were observable interactional effects between self-differentiation and covert narcissism to organization citizenship behavior.

• Toxicological Research
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• v.15 no.1
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• pp.19-25
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• 1999

(+)-Catechin inhibited the growth and induced the differentiation of HL-60 human leukimia cells. The degree of a differentiation by (+)-catechin during the differentiation, the expression assay, To understand the molecular mechanism of (+)-catechin during the differentiation, the expression level of oncogenes was detected by Northern blot analysis. c-Myc mRNA level was reduced after treatment with (+)-Catechin (10-4), however, the expression of c-jun was increased with a concentration dependent manner in HL-60 cells. These results showed that the differentiation and antiproliferation of HL-60 cells against (+)-Catechin was related to the reduction of c-myc and the induction of c-jun expression.

• Archives of Pharmacal Research
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• v.29 no.1
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• pp.40-45
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• 2006

Several diacetoxy acetal analogues have been synthesized from santonin and assessed for their ability of inducing or enhancing the differentiation of human HL-60 leukemia cells. The compounds themselves had little effect on HL-60 cell differentiation. However, three analogues, 2a, 3a, and 5b, synergistically enhanced 1,25-dihydroxyvitamin $D_3[1,25-(OH)_2D_3]-induced$ HL-60 cell differentiation when combined with 5 nM of dihydroxyvitamin $D_3[1,25(OH)_2O_3]$, a well-known differentiation inducer. Especially, the compound 5b profoundly enhanced the $1,25-(OH)2O_3]-induced$ HL-60 cell differentiation.