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Bioconversion of nitrogen oxides and reduction of ferric ions by probiotic lactic acid bacteria (프로바이오틱스 유산균에 의한 질소 산화물 전환 및 철 이온 환원활성)

  • Kim, Selim;Hong, Jungil
    • Korean Journal of Food Science and Technology
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    • v.53 no.6
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    • pp.785-791
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    • 2021
  • Many lactic acid bacteria (LAB) have probiotic properties that exert various health benefits. In this study, the reduction potential of nitrogen oxide compounds and ferric ions by six LAB, including Lactobacillus kimchicus, L. lactis, L. casei, L. plantarum, L. rhamnosus GG, and Leuconostoc mesenteroides were evaluated. The L. kimchicus strain produced a substantial amount of nitrite reduced from nitrate added to the media, whereas the other five LAB strains did not. L. kimchicus also showed the most potent reducing activity of ferric to ferrous ions. However, the reduction potential of the autoclaved L. kimchicus was little pronounced. The scavenging activities of viable LAB or their cell lysates against different radicals were not consistent with the potency of the LAB's reducing ability. The present results indicate that L. kimchicus has a strong reduction potential for nitrogen oxides in viable status, and that this ability can be used as a probiotic property for various health benefits.

A Study on the Changes in Perspectives on Unwed Mothers in S.Korea and the Direction of Government Polices: 1995~2020 Social Media Big Data Analysis (한국미혼모에 대한 관점 변화와 정부정책의 방향: 1995년~2020년 소셜미디어 빅데이터 분석)

  • Seo, Donghee;Jun, Boksun
    • Journal of the Korea Convergence Society
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    • v.12 no.12
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    • pp.305-313
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    • 2021
  • This study collected and analyzed big data from 1995 to 2020, focusing on the keywords "unwed mother", "single mother," and "single mom" to present appropriate government support policy directions according to changes in perspectives on unwed mothers. Big data collection platform Textom was used to collect data from portal search sites Naver and Daum and refine data. The final refined data were word frequency analysis, TF-IDF analysis, an N-gram analysis provided by Textom. In addition, Network analysis and CONCOR analysis were conducted through the UCINET6 program. As a result of the study, similar words appeared in word frequency analysis and TF-IDF analysis, but they differed by year. In the N-gram analysis, there were similarities in word appearance, but there were many differences in frequency and form of words appearing in series. As a result of CONCOR analysis, it was found that different clusters were formed by year. This study confirms the change in the perspective of unwed mothers through big data analysis, suggests the need for unwed mothers policies for various options for independent women, and policies that embrace pregnancy, childbirth, and parenting without discrimination within the new family form.

Comparative Analysis of the Factors Affecting on Youth's Civic Participation in the East Asian Countries (동아시아 청소년의 시민참여 영향요인 비교 분석)

  • Park, Kyunghee;Park, Hwanbo;Jeong, Seongkyeong
    • Korean Journal of Comparative Education
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    • v.28 no.4
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    • pp.189-220
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    • 2018
  • The purpose of this study is to analyze the effect of individual background, educational experiences, and social awareness on youth's civic participation in the three East Asian countries (South Korea, Hong Kong, and Taiwan), and provide educational implications for encouraging Korean youth's civic participation. And we estimated the effects of 17 variables on the youth's current and future civic participation using 'International Civic and Citizenship Education Study (ICCS) 2016' data opened in 2018. The result showed that the factors affecting on the civic participation were significantly different between the three countries. And significant differences on current and future civic participation were confirmed within the same country. First, Korean youth have high level of their parents' civic resources but the level of civic education in school is lower than other countries, and Taiwan youth have relatively high level of national identity. Second, the factors affecting on civic participation are more influenced by educational experiences than individual background such as parents' socioeconomic status, and the mass media have significant effect on youth's civic participation in all the three countries. Third, non-formal program and cultural experiences in school have significant effects on civic participation in future adulthood. Based on these results, this study suggested that it was important to provide various non-formal activities as well as expansion of civic education for encouraging youth's civic participation.

Distribution and Biodiversity of Lactic Acid Bacteria Having Bacteriocin-like Activity from Fresh Fruits and Vegetables (신선 과채류에 존재하는 박테리오신 유사 활성을 지니는 유산균의 분포와 다양성)

  • Park, Young-Seo;Jang, Jae Kweon;Choi, Young Jin;Chung, Myong-Soo;Park, Hoon;Shim, Kun-Sub
    • Food Engineering Progress
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    • v.13 no.1
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    • pp.64-69
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    • 2009
  • From the 25 fresh fruits and vegetable products, 1,250 isolates grown on MRS agar media were screened for the inhibitory activity on the growth of Escherichia coli 0157:H7, Listeria monocytogenes, and Bacillus cereus as well as Lactobacillus plantarum, L. casei, and Lactococcus lactis subsp. lactis. Among them, 607 isolates (49% of total isolates) from 23 different foods produced growth inhibitory activity on the E. coli 0157:H7, L. monocytogenes, or B. cereus. When these isolates were screened for the inhibitory activity on the growth of L. plantarum, L. casei, and Lactococcus lactis subsp., 24 isolates (3% of total isolates) from 7 food samples showed bacteriocin-like activity. These isolates had typical physiological characteristics of lactic acid bacteria, which indicated these isolates were strains of lactic acid bacteria. The inhibitor from 3 out of 24 revealed as nicin. From the RAPD-PCR profiles, 24 strains was classified and it was also indicated that most of the strains isolated from same produce showed similar phylogenetic profile.

Translation and Adaptation of the Children's Home Inventory for Listening Difficulties (CHILD) into Korean (가정환경 아동듣기평가(CHILD) 부모용 설문지의 한국어 번역 및 적용 연구)

  • Choi, Jae Hee;Seo, Young Ran;Jang, Hyun Sook
    • 재활복지
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    • v.20 no.4
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    • pp.247-264
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    • 2016
  • The Children's Home Inventory for Listening Difficulties (CHILD) questionnaire has been applied for assessing listening and communication difficulties in various home situations for children with hearing loss. The purpose of the study was to translate the CHILD questionnaire for parents into Korean and verify reliability and validity of Korean version of CHILD (CHILD-K). CHILD-K was completed by 55 parents of children (from ages 3~12 years) using cochlear implants (CI). Among the 55 children, 27 were in preschool and 28 in elementary. Internal consistency reliability of CHILD-K was verified by Chronbach's alpha. The mixed factorial ANOVA was conducted to compare the effects of the age group and situation factors (Quiet, Noise, Distance, Social, and Media factors) on the score of CHILD. The results indicated that CHILD-K showed excellent internal consistency reliability (${\alpha}=.96$). The CHILD scores among age groups were significantly different as the older age group resulted in higher scores in all situations except Distance. For both groups the mean scores for the Quiet situation were significantly higher than other situations, and the mean scores for the Social situation were significantly lower than other situations. Moreover, analysis showed that children with CI had difficulties in the Social situation combined with other situation factors. The results indicate that the Korean version of CHILD questionnaire is a reliable tool for the assessment of communication abilities in home situation in Korean-speaking children using CI.

A experimental Feasibility of Magnetic Resonance Based Monitoring Method for Underground Environment (지하 환경 감시를 위한 자기공명 기반 모니터링 방법의 타당성 연구)

  • Ryu, Dong-Woo;Lee, Ki-Song;Kim, Eun-Hee;Yum, Byung-Woo
    • Tunnel and Underground Space
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    • v.28 no.6
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    • pp.596-608
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    • 2018
  • As urban infrastructure is aging, the possibility of accidents due to the failures or breakdowns of infrastructure increases. Especially, aging underground infrastructures like sewer pipes, waterworks, and subway have a potential to cause an urban ground sink. Urban ground sink is defined just as a local and erratic collapse occurred by underground cavity due to soil erosion or soil loss, which is separated from a sinkhole in soluble bedrock such as limestone. The conventional measurements such as differential settlement gauge, inclinometer or earth pressure gauge have a shortcoming just to provide point measurements with short coverage. Therefore, these methods are not adequate for monitoring of an erratic subsidence caused by underground cavity due to soil erosion or soil loss which occurring at unspecified time and location. Therefore, an alternative technology is required to detect a change of underground physical condition in real time. In this study, the feasibility of a novel magnetic resonance based monitoring method is investigated through laboratory tests, where the changes of path loss (S21) were measured under various testing conditions: media including air, water, and soil, resonant frequency, impedance, and distances between transmitter (TX) and receiver (RX). Theoretically, the transfer characteristic of magnetic field is known to be independent of the density of the medium. However, the results of the test showed the meaningful differences in the path loss (S21) under the different conditions of medium. And it is found that the reflection coefficient showed the more distinct differences over the testing conditions than the path loss. In particular, input reflection coefficient (S11) is more distinguishable than output reflection coefficient (S22).

The Moderating Effect of Recognition of Employment Welfare Services in the Relationship between Age Norms and Negative Perception on Older Workers (연령규범과 고령근로자에 대한 부정적 인식의 관계: 고용서비스 인지의 조절효과)

  • Chung, Soondool;Lee, MiWoo;Chung, Semi
    • Survey Research
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    • v.18 no.3
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    • pp.47-76
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    • 2017
  • This study has been done to understand the relationship between age norms and negative perception on older workers, and to find out moderating effect of recognition of employment welfare services in those relationships. And the purpose of this study is to suggest alternative ways to improve pessimistic perception on older workers, which is basal ageism. For this purpose, the survey has been conducted with 630 participants, who were 20 years old and over and lived in 15 different provinces. The collected data has been analysed by using SPSS Win 18.0. The results as followed: first, there were significant differences of negative perception on older workers among provinces(metropolitan areas, small towns, and rural areas); second, age norms significantly influenced on pessimistic perception on older workers; third, moderating effect of recognition of employment welfare services has been verified. These results suggest that it is necessary to promote people to take a flexible attitude toward older adults using media broadcasting and promotion of employment welfare services. Public and private cooperation should be needed to change people's negative perception on older workers. Lastly, programs, which are appropriated and tailored in specific provinces, improving awareness of older workers should be required.

Reasonable Adjustment plan of Administrative boundary for Cadastral Re-examination district - Based on the result of Cadastral resurvey in Songgi-ri - (지적재조사사업지구 내 행정구역경계 합리적 조정방안 - 지적재조사사업 송기지구 중심으로 -)

  • Kim, Honng-Ryeol;Heo, Tae-Heon;Lee, Keon-soo
    • Journal of Cadastre & Land InformatiX
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    • v.48 no.2
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    • pp.31-49
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    • 2018
  • The boundaries of administrative districts are the basic system for the whole country and are closely related to the daily life of the residents. The administrative boundary of Korea is registered and managed in the cadastral study based on the intellectual study of cadastral system. There is a non-conforming land where the registration details of some cadastral studies do not match the actual situation of the land, and the cadastral rehabilitation project is implemented as a national project to create a new digital cadastral study. However, due to the cadastral rehabilitation project, there is an unreasonable parcel that the past administrative boundaries and newly registered boundaries are different from each other due to the registration of the past administrative boundaries for the newly registered cadastral studies. The purpose of this study is to identify the unreasonable administrative boundaries arising from the cadastral rehabilitation project and to identify problems in order to effectively manage the number of branches in terms of administrative and administrative efficiency in terms of registration and inhabitants management. It is expected that the legal system will be improved to legitimately control the boundary of the administrative district and to manage the boundaries of the administrative district accordingly and to provide the service and to solve the inconvenience of the nation.

Investigation of Microbial Communities in Sulculus diversicolor supertexta Through 16S rRNA Sequencing and Antibacterial Monitoring of Harmful Strains (16S rRNA 염기서열 분석을 통한 오분자기(Sulculus diversicolor supertexta)내 미생물 군집 조사 및 인체유해 질병세균에 대한 항균활성 모니터링)

  • Kim, Min-Seon;Lee, Seung-Jong;Heo, Moon-Soo
    • Journal of Life Science
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    • v.28 no.12
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    • pp.1477-1488
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    • 2018
  • This study investigated the muscles, intestines, and gonads of Sulculus diversicolor supertexta to examine the diversity of microbial communities within examples collected from the Jeju Coast. Using different media, initial pure isolation in MA, 1% BHIA, and 1% TSA indicated that the muscles, intestines, and gonads supported more communities, respectively. In analysis of relative similarity with 16s rRNA sequencing, 190 pure colonies were isolated, and further analysis with NBLAST identified 71 species, 39 genera, 25 families, and five phyla. Homogeny with the reference strain was 91-100%. Microbial communities in S. supertexta consisted of gamma and alpha Proteobacteria (48%), Actinobacteria (32.5%), Firmicutes (16.9%), Deinococcus-Thermus (1.3%), and Bacteroides (1.3%). In all tissue, Psychrobacter cibarius in Moraxellaceae was dominant. Alteromonadaceae, Enterobacteriaceae, Pasturellaceae, Moraxellaceae, Rhodobacteraceae, Geminicoccaceae, Dietziaceae, Intrasporangiaceae, Microbacteriaceae, Micrococcaceae, Micromonosporaceae, Streptomycetaceae, Aerococcaceae, Bacillaceae, Paenibacillaceae, Planococcaceae, and Staphylcoccaceae were commonly isolated across all tissues, and Flavobacteriaceae, Corynebacteriaceae, Yesiniaceae, Vibrionaceae, Hahellaceae, Pseudomonadaceae were also identified from the intestines. In microbial monitoring of four harmful bacteria, Streptomyces albus (96%) showed antibacterial activity against all four strains, and Agrococcus baldri (99%) and Psychrobacter nivimaris (99%) presented against E. Coli and E. aerogens. In addition, some strains with low homogeny were isolated and further experiments are therefore required, for example to refine the antimicrobial substances including new strain investigations. These additional experiments would aim to establish generic resources for the microbial communities in S. Supertexta and provide basic data for applied microbiological research.

Evaluation of the Discoloration of Pyropia yezoensis Ueda Using a Colorimeter (색차계를 이용한 방사무늬김(Pyropia yezoensis Ueda)의 황백화 평가)

  • Lee, Sang Yong;Kim, Young Hee;Lee, Jee Eun;Hwang, Eun Kyoung
    • Korean Journal of Environmental Biology
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    • v.36 no.4
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    • pp.620-625
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    • 2018
  • Pyropia yezoensis is one of the most extensively cultivated seaweeds used as food in Korea. However, the quality and yield of Pyropia farms are frequently affected by outbreaks of thallus discoloration. The objective of this study was to develop a simple and rapid method to evaluate P. yezoensis discoloration caused by nutrient deficiencies. A colorimeter was used to quantify the color of P. yezoensis in nutrient deficient media over 10 days. Quantitative values of lightness (L), redness (a), and yellowness (b) measured with a colorimeter were used as indicators of P. yezoensis discoloration. The vacuoles of P. yezoensis were observed at 4 days and swelled gradually from 6 days onward. The three colorimeter parameters L, a, and b values showed significantly different values with culture period. The lightness (L) value was darker initially, and continued to lighten over culture period. The yellowness (b) value increased, but the redness (a) value decreased throughout this study. The a/b ratio and redness (a) values from the colorimeter were important P. yezoensis color change indicators. The discoloration group of P. yezoensis had quantitative values for parameters of L above 70, a below 2.6, and b above 15.0. The results of this study showed that quantitative colorimetric measurements can be used as tools for simple and rapid detection of P. yezoensis discoloration.