• Journal of Plant Biotechnology
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• 제36권3호
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• pp.219-223
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• 2009

Oxidative stress derived from excess reactive oxygen species (ROS) is a major damaging factor for plants exposed to environmental stresses. Sweetpotato [Ipomoea batatas (L.) Lam] has a relatively broad adaptability to harsh environmental conditions compared to other staple crops. In this study, to select stress-tolerant sweetpotato cultivars for sources of molecular breeding on marginal lands, we evaluated the ion leakage values in 10 different cultivars after treatment of methyl viologen (MV), an ROS-generating nonselective herbicide, to leaf discs. DPPH radical scavenging activity and the contents of total phenolics were also investigated. The ion leakage of each cultivar showed a diverse value, which is well correlated with DPPH radical scavenging activity of each cultivar. DPPH radical scavenging activity also showed a high corelation with the contents of total phenolic contents. Three cultivars of Yanshu 8, Shinhwangmi and Shinzami showed high antioxidant activity. Our results suggest that a simple and efficient DPPH radical scavenging activity would be a suitable method to select potential cultivars with enhanced tolerance to multiple environmental stress.

• Preventive Nutrition and Food Science
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• 제15권2호
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• pp.152-158
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• 2010

This study was designed to determine the best cultivar of kohlrabi for making pickles among KM-1 (Worldcol), KM-2 (Bejo), KM-3 (Takii), and KM-4 (Monsanto) cultivars. The initial pH of pickles was different in all cultivars; however, they all had a pH in the range of 3.81~3.86 after 28 days of storage. The pickles made from KM-4 had the lowest acidity, while there was no significant difference in acidity among the KM-1, KM-2, and KM-3 pickles. The salinity of all kohlrabi pickles was consistent during the storage period. The changes of color values were accelerated in the following order: KM-4>KM-1>KM-2>KM-3. Though kohlrabi pickles were non-fermented, the change in reducing sugar contents was similar to that of a fermented pickle. The highest value of reducing sugar content was found in pickles made with KM-4. In all cases, the firmness of the pickle decreased as time of storage increased. On the initial day, KM-4 pickle showed the highest firmness with 807.4 g. KM-4 also showed the highest firmness on the 28th day of storage with 602.3 g. In sensory evaluation, the KM-4 pickle exhibited the highest texture (hardness) among the pickles. The pickle made with the KM-4 cultivar showed relatively higher firmness of texture than other cultivars, suggesting that KM-4 could be utilized in other kinds of processed foods, in addition to being pickled.

• 한국작물학회지
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• 제45권1호
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• pp.14-19
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• 2000

In order to develop an appropriate cultural practice for a newly bred 2-rowed, waxy, naked barley cultivar 'Duwonchapssalbori' in the southern region, 2 varieties, 3 seeding dates, 4 seeding rates were designed with 2 replications. The heading and maturity dates of 'Duwonchapssalbori' were' earlier than 'Saessalbori by 2-5 and 4 days, respectively. Culm length of 'Duwonchapssalbori' was 60 to 66cm with lodging resistance. Grain yields of both varieties were 4.28-4.33 ton/ha in the plot of Oct. 20 seeding, 4.11-4.18 ton in the plot of Oct. 28 seeding, and 3.7-13.89 ton/ha in the Nov. 5 seeding. Grain yield with different seeding rates highly significantly fitted with secondary polynomial equations in the plots of Oct. 20 and Oct. 28 seedings ($R^2$=0.84-0.85), showing the greatest yield with seeding rate of 140kg per ha. However, the grain yield with various seeding rates in the plot of Nov. 5 seeding showed simple linear regression ($R^2$=0.71), showing the seeding rate over 160kg in the case of later seeding to be optimal. Number of spikes per $m^2$ significantly contributed to grain yield, and fitted to simple linear regression ($R^2$=0.881-0.891), suggesting that plenty of early stands should be established at early seedling stage for high grain yield. Young spikes of 'Duwonchapssalbori' in the plot of Oct. 20 seeding elongated with linear increments since Feb. 10 (6.7mm), and showed rapid increment since March 7 (15.1mm). However, elongation of young spikes of 'Saessalbori' began from March 15 with slow increments. From these studies, the optimum seeding date and seeding rate in 'Duwonchapssalbori' were Oct. 20-28 and 140kg per ha for high grain yield in the southern regions.

• 한국식품영양학회지
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• 제33권3호
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• pp.279-286
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• 2020

In this study, the properties of the substances fermented soybean producted by manufacturing with several Aspergillus strains were investigated. The five soybean cultivar that includes miso, jinpung, pyeongwon, cheonga and chamol were used in this experiment. The pH and total acidity were 6.30~6.66%, and 0.27~0.48%, respectively with differences depending on the samples. The color values for L value, a, and b value were 60.28~69.80, 0.64~3.68, and 11.48~31.21, respectively. The amylase activities, protease activities, and amino-type nitrogen of the jinpung samples were the highest among all cultivars. The mold counts the fermented soybean products by cultivars were 6.18~9.14 log CFU/g, and miso was the highest. Free amino acid and organic acid contents were highest in the jinpung and showed different composition depending on each sample. A total of 18 volatile aroma-compounds, including two acids, four alcohols, four ketones, three phenols, one furan, three pyrazines, and one miscellaneous compounds. In conclusion, it is expected that manufacturing A. oligze inoculation fermented soybean products using jinpung cultivar will improve quality.

• The Plant Pathology Journal
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• 제19권6호
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• pp.269-273
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• 2003

A total of 57 isolates of Colletotrichum gloeosporioides were recovered from diseased tissues of Hall's crab apple (Malus haliana), 3 cultivars of edible apple (M. pumila var. dulcissima), red pepper (Capsicum annum), and grapevine (Vitis vinifera) fruits. All isolates showed strong virulence on their own host plants. Isolates from edible apple exhibited high level of cultivar specificity in pathogenicity tests. Ten isolates from apple cultivar 'Fuji' were virulent on 'Jonathan' and 'Rall's Genet'. However, 12 isolates from 'Jonathan' and 'Rall's Genet' were not virulent on 'Fuji'. Among the 24 isolates from red pepper, only seven and two isolates were infective on edible apple and grapevine fruits, respectively. All six isolates from grapevine were only virulent on their own host. These isolates were grouped into five vegetative compatibility groups (VCGs), A, B, C, D, and E, by demonstrating heterokaryosis through complementation using nitrate-nonutilizing (nit) mutants. Among them, isolates belong to VCG-A and VCG-D accounted for 24 and 17 isolates； those in VCG-A exhibited wide host range involving Hall's crab apple, all three edible apple cultivars, and red pepper. On the other hand, isolates of VCG-D and VCG-E showed limited host range specific to red pepper and grapevine, respectively. Taken together, the data suggest that among C. gloeosporioides isolates, the concepts of pathotype and/or forma specialis may exist, and that three is a relationship between host specificity and VCG grouping among C. gloeosporioides isolates.

• Plant Biotechnology Reports
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• 제4권1호
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• pp.23-27
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• 2010

Quantitative trait loci (QTLs) controlling ability of somatic embryogenesis were identified in soybean. A frame map with 204-point markers was developed using an RI population consisting of 117 $F_{11}$ lines derived from a cross between cultivar 'Keburi' and a weedy soybean 'Masshokutou Kou 502'. The parents differed greatly in their abilities of somatic embryogenesis using immature cotyledons as explants. The ability of somatic embryogenesis was evaluated in five different experiments: the $F_{11}$ (evaluated in 1998) and $F_{15}$ (2002) generations cultured on basal media supplemented with $40\;mg\;l^{-1}$ 2,4-D (2,4-D1998 and 2,4-D2002), $F_{14}$ (2001) generation on medium with $40\;mg\;l^{-1}$ 2,4-D and high sucrose concentration [2,4-D2001 ($30\;g\;l^{-1}$ sucrose)], and the $F_{11}$ (1998) and $F_{12}$ (1999) generations on medium with $10\;mg\;l^{-1}$ NAA (NAA1998 and NAA1999). The RILs showed wide and continuous variations in each of the five experiments. In the composite interval mapping analysis, 2 QTLs were found in group 8 (D1b + W, LOD = 5.42, $r^2$ = 37.5) in the experiment of 2,4-D1998 and in group 6 (C2, LOD = 6.03, $r^2$ = 26.0) in the experiment of 2,4-D2001 (high concentration sucrose). In both QTLs, alleles of 'Masshokutou Kou 502' with high ability of somatic embryogenesis contributed to the QTLs. For the other three experiments, no QTL was detected in the criteria of LOD >3.0, suggesting the presence of minor genes.

• 한국작물학회지
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• 제50권1호
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• pp.1-4
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• 2005

This experiment was conducted to investigate the soil nitrogen credit of biological nitrogen fixation (BNF) and the nitrogen balance of soybean in soybean-barley cropping systems. Soybean cultivar, Shinpaldalkong2 and barley cultivar, Olbori, were used in soybean mono-cropping (SM), barley monocropping (BM), and barley­soybean double cropping system. The barley-soybean double cropping system was treated with two different levels of nitrogen fertilizers, 0 nitrogen fertilizer (BS-F0), and standard nitrogen fertilizer (BS-F1). Nitrogen and organic matter concentrations in soil of BS-F1 plot on October, 2001 were increased $4.8\%\;and\;5.9\%$, respectively, compared with those on October, 2000. The ranges of BNF rate in soybean were $69.1\~ 88.2\%$ in two years, and the rate was the highest in BS-F0 plot and the lowest in SM plot. The ranges of nitrogen harvest index (NHI) in all treatments were $83.9\~86.7\%$. The yield was 270 kg/10a in BS­F1 plot and 215 kg/10a in BS-F0 plot. However, the nitrogen balances were +0.6 kg/10a of gain of soil nitrogen in BS-F0 plot and -0.4 kg/10a of loss of soil nitrogen in BS-F1 plot. In comparisons of SM and BS-F1 plots, although the seed yields were similar in two plots, the loss of soil nitrogen was higher in SM than BS-F1 plot. Overall, our results suggest that barley-soybean double cropping system was more effective in respect to seed productivity and soil nitrogen conservation than soybean monocropping system, and the N credit to following crops by soybean cultivation was identified in soybean double cropping system.

• 한국작물학회지
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• 제24권2호
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• pp.75-81
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• 1979

우리나라 장여품종을 위함한 15개 대두품종들의 근류균에 대한 친화성과 접종효과를 밝히기 위하여 사경시험을 실시하였던 바 그 얻어진 결과를 요약하면 다음과 같다. 1. 정석은 근류균접종 15일후에도 근류가 형성되지 않았으나 Lindarin #62는 주당 3.0개의 근류가 형성 되었으며 개화기의 근류형성수도 품종간에 크게 차이가 있어서 정석은 주당 37.8대로 가장 적었고 Haman은 주당 122.2개로 가장 많이 형성되었는데 품종간에 유의차가 인정되었다. 2. 개화기까지의 근류형성수에 대한 시기별 근류형성비율은 품종간에 크게 차이가 있었는데 공시품종평균에 있어서는 접종 15일후에 2,1％, 접종30일후에는 60.8％, 그리고 접종 45일후에는 79.8％가 형성되었다. 3. 근류병접종효과는 현저하여 초장, 경태, 근장, 건물중 및 엽록소함량이 무접종구에 비하여 접종구가 크게 증가하여 전체처리 및 품종간, 그리고 접종처리간에 고도로 유의하였는데 다만 엽록소함량과 건물중의 증가추세와는 반드시 일치되지 않았다. 4. 공시품종평균에 있어서 접종구에 대한 무접종구의 몇가지 현질의 형성비율은 초장 94.0％, 건물중 81.6％, 그리고 엽록소의 함량은 66.8％였다.

• 생명과학회지
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• 제20권1호
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• pp.142-146
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• 2010

친환경적으로 우수한 품종을 찾고자 4개 시비 처리구와 1개 품종, '보석찰'과 재래종 녹색현미찰로부터 선발한 3 계통으로 실험을 행하였다. 각 비료시비구당 계통별 분얼수는 GG-05-03과 GG-05-04는 유사하고 GG-05-07은 적었다. 계통별 초장은 '보석찰'에 비해 GG-05-04와 GG-05-07은 다소 크고, GG-05-03 유사하였다. 이삭당 낟알의 무게와 낟알수는 계통별 큰 차이가 없었다. 임실율은 계통별 차이가 없었으나 천립중의 경우 '보석찰' 보다는 가벼웠다. 수량은 '보석찰'과 비교하였을 경우 GG-05-03과 GG-05-07은 다소 적고, GG-05-04는 유사하였다. 미질은 '보석찰'과 비슷하고 조지방에서 차이를 다소 보였다. 이상의 결과를 요약하면 GG-05-07 계통이 보다 친환경적 재배에 유용한 것으로 나타났다.

• Journal of Microbiology
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• 제44권3호
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• pp.363-368
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• 2006

In this study, native Byadyrhizobium strains were isolated from the host plant, Glycine max, harvested from fields in Madhya Pradesh, India, and were typed by Iytic rhizobiophages. Eight indigenous (Soy2, ASR011, ASR031, ASR032, MSR091, ISR050, ISR076 and ISR078) and two exotic strains (USDA123 and CB1809), all of which evidenced a distinct reaction with six phages, were employed in this study. The symbiotic interaction of these strains was studied initially using soybean cultivar JS335 in a sand culture in a controlled environment, and the efficiency was assessed based on the nodule number, nodule dry weight, plant dry weight, nitrogenase activity, and total accumulation of N per plant. Symbiotic effectiveness was found to be highest with the native phage-sensitive isolate ASR011, whereas it was at a minimum with the phage-resistant isolates, ISR050 and ISR078. Additionally, the effectiveness of these strains was evaluated using six soybean cultivars belonging to different maturity groups; namely, Brags, Lee, Pusa20, PK416, JS33S and NRC37. Analysis of variance data evidenced significant differences due to both symbionts, for the majority of the tested parameters. The CB1809, USDA123, and ASR011 strains evidenced relatively superior symbiotic effectiveness with soybean cultivars Brags, Lee and JS335. Strain ISR078 evidenced no significant responses with any of the cultivars. The ASR031 strain performed moderately well with all tested cultivars. The symbiotic response of all the strains was quite poor with cultivar PK416. Our studies showed that a significant relationship existed between the phage sensitivity and symbiotic efficiency of the bacterial strains with the host-cultivars.