• The Journal of Internal Korean Medicine
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• v.19 no.1
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• pp.431-441
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• 1998

This study was undertaken to determine whether Buthus exract(BTE) affects Na^+-K^+-ATPase$ activity of nervous tissues. The enzym activity was measured in synaptosomal fraction prepared from rabbit brain cortex. Na^+-K^+-ATPase$ activity was inhibited by BTE over concentration range of 0.05-0.5% in a dose-dependent manner. The enzyme activity was increased by an increase in $Na^+$ concentration from 5 to 100mM, $K^+$ concentration from 0.5 to 10mM, and $Mg^{2+}$ concentration from 0.2 to 5mM. These changes in ion concentrations did not produce any effect on the inhibitory effect of BTE on $Na^+-K^+-ATPase$ activity. An increase in ATP concentration from 0.1 to 3mM caused an increase in the enzyme activity. The inhibition of the enzyme activity by BTE were not different between two ATP concentrations. A sulfhydryl group protector DTT prevented PCMB-induced inhibition of $Na^+-K^+-ATPase$ activity, but the BTE-induced inhibition was not altered by DTT. The inhibition of enzyme activity by combination of ouabain and BTE was not different from that by Buthus alone. These results suggest that Buthus exerts inhibitory effect on $Na^+-K^+-ATPase$ activity in cerebral synaptosomes, and the action mechansim is similar to that of ouabain.

• Journal of Environmental Science International
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• v.26 no.12
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• pp.1375-1387
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• 2017

In order to investigate the $PM_{10}$ concentration trend and its characteristics over five different sub area in Busan from 2013 to 2015, data analysis with considering air flow distribution according to its topography was carried out using statistical methodology. The annual mean concentrations of $PM_{10}$ in Busan tend to decrease from $49.6{\mu}g/m^3$ in 2013 to $46.9{\mu}g/m^3$ in 2015. The monthly mean concentrations value of $PM_{10}$ were high during spring season, from March to May, and low during summer and fall due to frequent rain events. The concentration of $PM_{10}$ was the highest in five different sub-area in Busan. High concentration episodes over 90 percentile of daily $PM_{10}$ concentration were strongly associated with mean daily wind speed, and often occurred when the westerly wind or southwesterly wind were dominant. Regardless of wind direction, the highest correlation of $PM_{10}$ concentrations was observed between eastern and southern regions, which were geographically close to each other, and the lowest in the western and eastern regions blocked by mountains. Wind flow along the complex terrain in Busan is also one of the predominant factors to understand the temporal variation of $PM_{10}$ concentrations.

• Journal of Mushroom
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• v.3 no.2
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• pp.65-70
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• 2005

This study was carried out to select oyster mushroom strains tolerant to salinity and characteristic cultivation by different NaCl concentration. Among the 64 strains, Kimjae-10ho, Nongki-2-1ho, Myungyul, Byungneutari-1ho, Bupyungsoyae-1ho, Sambok and Chunchu-2ho resulted in higher mycelial growth and density on PDA medium containing NaCl. The maxium NaCl concentration possible to fruit body formation was 0.5% NaCl in Myungyul, 1.0% NaCl in Kimjae-10ho and Bupyungsoyae-1ho, 1.5% NaCl in Nongki-2-1ho, Byungneutari-1ho, Sambok and Chunchu-2ho, respectively. Increased NaCl concentration on sawdust medium prolonged culture period, while decreased total quantity and come to be short and thin in Length and thickness of stipe. Higher NaCl concentration in the medium decreased the uptake rate of $K_2O$ and CaO, whereas increased the NaCl uptake rate.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.2
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• pp.139-142
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• 1984

Short-term acute toxicity of synthetic detergent(LAS) to larvae of loach, Misgurnus angillicaudatus was examined by static bioassay. The larvae were exposed to 15 different concentration of synthetic detergent for 16, 48, 72, 96 and 120 hours in order to determine median lethal concentration($LC_{50}$). The $100\%$ mortarlity of larvae was showed within 120, 96, 48 and 16 hours for 6, 18, 30 and 38 ppm, respectively. The median lethal concentration values of the larvae were 12.59 ppm for 48 hours, 4.00 ppm for 96 hours and 1.02 ppm for 120 hours. The permissible toxicant concentration of acute toxicity to larvae was $0.37{\sim}0.43$ ppm, and application factor of the synthetec detergent was $0.093{\sim}0.108$. The median lethal time($LT_{50}$) for different concentration also was determined. The $LT_{50}$ of 0.2 ppm was found within 165.1 hours and 2 ppm was 106.2 hours, while the $LT_{50}$ of 8 ppm was 60.3 hours and that of 38 ppm was 23.5 hours.

• Journal of the Korean Society of Tobacco Science
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• v.24 no.1
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• pp.38-45
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• 2002

This study was conducted to determine the smoke component concentration and the smoke distribution in the cambridge filter pad and cigarette filter with stalk position and grade of Korean leaf tobaccos. 12 grades of flue-cured and burley leaf tobaccos which were taken from the Kim-cheon and Ock-cheon leaf tobacco processing factory were used for this study. Six kinds of smoke components such as tar, nicotine, water, carbon monoxide(CO), carbon dioxide($CO_2$) puff No. collected in the cambridge filter pad and cigarette filter were analyzed. Also, nicotine/tar, CO/tar ratio were calculated from the analytical data. Puff no., CO, and $CO_2$ concentration of flue-cured and burley tobacco leaves at high stalk position were higher than those of low stalk position, and these components were increased in high grade of leaf tobacco. Compared with flue-cured tobacco, burley tobacco showed a little values of puff no., nicotine, and tar concentration. The pH range of burley tobaccos was broader than that of flue-cured tobaccos, and the ammonia concentration of burley tobaccos was significantly higher than that of flue-cured tobaccos. The pH value and ammonia concentration of burley tobaccos were decreased as stalk position of leaf tobaccos decreased. The distribution of smoke components collected in the cambridge filter pad and cigarette filter was different with a different grade and stalk position of tobacco leaves. In high stalk position of tobacco leaves, the portion of smoke components collected in the cambridge filter pad was also increased. The average nicotine, water, and tar removal efficiency by a cigarette filter in a flue-cured tobacco were 37%, 64%, and 48%, respectively. Compared with flue-cured tobaccos, smoke components of burley tobaccos had high nicotine and low water removal efficiency by a cigarette filter. The average ratio of nicotine/tar and CO/tar of flue-cured tobaccos and burley tobaccos were 0.097, 1.22, 0.094, and 0.97, in order. Nicotine/tar ratio was decreased and CO/tar ratio was increased in low stalk position.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.10
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• pp.672-678
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• 2014

In this study, variations of fermentative hydrogen production and microbial community were investigated with different nitrogen concentration of food waste. Optimum hydrogen production rate was acquired at 200 mg/L nitrogen concentration of the food waste. Which was eqivalent to 83.43 mL/g dry biomass/hr. However, bio-hydrogen production was inhibitedly reduced at over 600 mg/L of nitrogen concentration whereas proportional relation between hydrogen production and B/A ratio were not observed. Most dominant specie of the microbial community analyzed was Clostridium sp. throughout PCR-DGGE analysis of 16S rDNA. It revealed that most contributing microorganism producing hydrogen were Enterococcus faecium partial, Klebsiella pneumoniae strain ND6, Enterobacter sp. NCCP-231, and Clostridium algidicarnis strain E107 in this experiment.

• Physical Therapy Korea
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• v.14 no.2
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• pp.37-43
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• 2007

Electrical acupuncture has been shown to induce hypoglycemic effect in streptozotocin diabetic rats. The Zusanli acupoints have been widely used in traditional Chinese medicine to relieve symptoms of diabetes mellitus. But, the effects of electrical acupuncture stimulation of different frequencies are still unclear. We designed an experiment to investigate the effect of electrical acupuncture of high (100 Hz) and low (2 Hz) frequencies on Zusanli acupoint for 15 minutes in streptozotocin diabetic rats. They were divided into 4 groups, high frequency electrical acupuncture (HFEA group, n=5), low frequency electrical acupuncture (LFEA group, n=5), without any treatment (control group, n=5) and normal group (n=5). After 2 weeks of treatment, the HFEA and LFEA groups had a significantly decreased glucose concentration in serum in comparison to the control group. Additionally, the HFEA group had a significantly decreased glucose concentration in serum compared with the LFEA group. Both the HFEA and LFEA groups had a significantly decreased Immunoglobulin G concentration in serum compared with control group. The LFEA and control groups had a significantly increased cholesterol concentration in serum compared with the normal group, but the HFEA did not have a significantly increased cholesterol concentration. Thus we suggest that electrical acupuncture stimulation at the Zusanli acupoints is effective on hypoglycemic effect and immune function. Overall, the therapeutic effects of electrical acupuncture at 100 Hz was better than effects at 2 Hz.

• Journal of Periodontal and Implant Science
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• v.42 no.6
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• pp.224-230
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• 2012

Purpose: Various bone graft materials have been used for periodontal tissue regeneration. Demineralized freeze-dried bone allograft (DFDBA) is a widely used bone substitute. The current widespread use of DFDBA is based on its potential osteoinductive ability. Due to the lack of verifiable data, the purpose of this study was to assess the osteoinductive activity of different DFDBAs in vitro. Methods: Sarcoma osteogenic (SaOS-2) cells (human osteoblast-like cells) were exposed to 8 mg/mL and 16 mg/mL concentrations of three commercial types of DFDBA: Osseo+, AlloOss, and Cenobone. The effect of these materials on cell proliferation was determined using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. The osteoinductive ability was evaluated using alizarin red staining, and the results were confirmed by evaluating osteogenic gene expression using reverse transcription polymerase chain reaction (RT-PCR). Results: In the SaOS-2 cells, an 8 mg/mL concentration of Osseo+ and Cenobone significantly increased cell proliferation in 48 hours after exposure (P<0.001); however, in these two bone materials, the proliferation of cells was significantly decreased after 48 hours of exposure with a 16 mg/mL concentration (P<0.001). The alizarin red staining results demonstrated that the 16 mg/mL concentration of all three tested DFDBA induced complete morphologic differentiation and mineralized nodule production of the SaOS-2 cells. The RT-PCR results revealed osteopontin gene expression at a 16 mg/mL concentration of all three test groups, but not at an 8 mg/mL concentration. Conclusions: These commercial types of DFDBA are capable of decreasing proliferation and increasing osteogenic differentiation of the SaOS-2 cell line and have osteoinductive activity in vitro.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.2
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• pp.492-500
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• 1999

The aim of this study was to assess the association between total vitamin A, retinol and carotene intakes and serum concentration of total carotenoids in Korean male smokers and nonsmokers. Food frequency and nutrient intakes of 64 healthy college students were estimated by the 24 hour recall method and food frequency questionnaire and analyzed by smoking status. Serum total cartenoids and lipid profiles were measured in 28 smokers and 36 nonsmokers. The mean total vitamin A, retinol and carotene intakes of smokers were not significantly different from nonsmokers. However, carotene intakes of the smokers who have smoked more than 5 packyears were significantly lower than those of nonsmokers. Smokers consumed green yellow leafy vegetables more often(P=0.005) and fresh fruits less often(P=0.017) than nonsmokers. The serum total carotenoids of smokers consuming the same amount of retinol and carotene as nonsmokers, 1.79 g/ml, were 17% lower than nonsmokers, 2.15 g/ml(P<0.05). The lipid profiles including total cholesterol, LDL cholesterol, VLDL cholesterol and triglycerides of smokers were not significantly different from nonsmokers, however, the concentration of HDL cholesterol of smokers were significantly higher than that of nonsmokers. The serum carotenoids concentration was positively associated with fruit consumption frequency in smokers, and also was positively associated with green yellow leafy vegetable consumption frequency in non smokers. There were no correlations between dietary and serum carotenoids for smokers as well as nonsmokers. It was concluded that smoking could be inversely related to the serum concentration of carotenoids at constant carotene intakes and smokers might require more carotene to reach the same concentration comparable to nonsmokers.

• Journal of Integrative Natural Science
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• v.11 no.1
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• pp.30-38
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• 2018

CALPUFF is one of the recommended air pollution models by EPA with AERMOD. It has been used to simulate the ambient concentration of critical air pollutants as well as non-critical pollutants such as persistent organic matters and the organic materials causing odor. In this model, the air pollutants go through dispersion, transportation, chemical reaction, and deposition process. These mechanisms are significantly influenced by meteorological condition. This study produces the meteorological field in three different methods for the simulation of $SO_2$ using CALPUFF: 1) CALMET model by using both ground-level and aerological observation, 2) CALMET model by using MM5 results with NCEP/NCAR reanalyzed data, 3) CALMET model by using MM5 results in which FDDA is applied with NCEP/NCAR reanalyzed data as well as the meteorological data of Korea Meteorological Administration. As a result of CALPUFF model, the resolved concentration of $SO_2$ showed different behaviors in three cases. For the first case, the fluctuation of SO2 concentration was frequently observed while the fluctuation is reduced in the second and third cases. In addition, the maximum concentration of $SO_2$ in the first case was about 2~3 times higher than the second case, and about 4~6 times higher than the third case. These results can be caused by the accuracy of the resolved meteorological field. It is inferred that the meteorological field of the first case could be less accurate than other two cases. These results show that the use of correct meteorological data can improve the result of dispersion model. Moreover, the contribution of various sources such as point, line, and area sources on the ambient concentration of air pollutant can be roughly estimated from the sensitivity analysis.