• Journal of Nutrition and Health
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• v.29 no.6
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• pp.578-589
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• 1996

This study was performed to invstigate the effect of dietary protein level and source on cadmium intoxicification in rats. Forty-eight male rats of Sprague-Dawley strain weighing 171$\pm$3g were blocked into 8 groups of 6 animals according to body weigth, and were raised for 30days. Eight experimental diets different with cadmium(0ppm, 400ppm)and protein(15%, 40%) levels and protein source[casien, I.S.P.(isolated soy protein)] were given to animals for 30days. Food intake, weight gain, food efficiency ratio, liver weight, kidney weight and femur weight were lower in cadmium added group, and higher in high protein groups(40% protein) than medium protein groups(15% protein). But, dietary protein source had no influence on them. Cadmium concerntration of liver was higher in rats fed casein than I.S.P. groups, and cadmium concentration in intestine was higher in high protein groups. In femur both high protein and I.S.P.diets increased cadmium concentrations. MT concdentrations in liver, kidney and intestine were higher in cadmium added group, and kidney intestine MT concentration were higher in high protein group. Absorption and retention rates of cadmium were lower in rat fed I.S.P. than animal fed casein among medium protein groups and cadmium concentration in blood and liver of I.S.P groups were lower than casein groups. But absorption and retention rates of cadmium were similar in high casein and I.S.P. groups. Renal damage by cadmium administration was not seen in all groups. Absorption rates of zinc and copper competing with cadmium in absorption process were lower in high protein groups than medium protein groups and lower in rats fed I.S.P. than casein. In conclusion, weight gain, F.E.R, and MT concentraion of high protein groups were higher than those of medium protein groups and absorption and retention rates of cadmium were lower in high protein groups. From these results, it was shown that cadmium toxicity was alleviated by high dietary protein. Meanwhile, the effect of dietary source on the cadmium toxicity was different with protein level. In medium protein groups absorption and retention rates of cadmium were much lower in rats fed I.S.P. than casein. In high protein groups, cadmium toxicity was not influenced by protein source and absorption and retention rates of cadmium were not different between casein and I.S.P. groups.

• Journal of Nutrition and Health
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• v.46 no.5
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• pp.447-460
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• 2013

Dietary supplement use is prevalent and represents an important source of nutrition. This study was conducted in order to assess the dietary maximum exposure of vitamins and minerals from various sources including regular diet, vitamin mineral supplements for non-prescription drug (VMS-NPD), vitamin mineral supplements for health functional foods (VMS-HFF), and fortified foods (FF). A total of 1,407 adolescent boys and girls attending middle or high schools were chosen from various cities and rural communities in Korea. Users of vitamin and mineral supplements (n = 60, 15-18 years of age) were chosen from the above 1,407 students. Intake of vitamins and minerals from a regular diet and FF was assessed by both food record method and direct interview for three days of two weekdays and one weekend, and those from VMS-NPD and VMS-HFF were assessed by both questionnaire and direct interview, and compared with the recommended nutrient intake (RNI) and the tolerable upper intake level (UL) for Korean adolescents. Daily average exposure range of vitamins and minerals from a regular diet was 0.3 to 4.4 times of the RNI. Some subjects had an excessive exposure to the UL in the following areas: from regular diets, vitamin A (1.7%) and niacin (5.0%); from only VMS-NPD, vitamin C (9.1%) and iron (5.6%); and from only VMS-HFF, niacin (8.6%) > vitamin $B_6$ (7.5%) > folic acid (2.9%) > vitamin C (2.3%). Nutrients of daily total intake from regular diet, VMS-NPD, VMS-HFF, and FF higher than the UL included nicotinic acid for 33.3% of subjects, and, then, in order, vitamin C (26.6%) > vitamin A (13.3%), iron (13.3%) > zinc (11.7%) > calcium (5.0%) > vitamin E (1.7%), vitamin $B_6$ (1.7%). Thus, findings of this study showed that subjects may potentially be at risk due to overuse of supplements, even though most of them took enough vitamins and minerals from their regular diet. Therefore, we should encourage adolescents to have sound health care habits through systematic and educational aspects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.4
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• pp.451-461
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• 2009

The purpose of this study is to provide fundamental information for snack development contributing to physical growth of children with cerebral palsy. The study was conducted on the children with cerebral palsy of age 1 to 7 by investigating their food behavior, physical growth development, nutritional status, and snack intake. As a result of assessing physical growth by WLI (Weight-Length Index), the proportions of the children with cerebral palsy were the following: underweight 45.5%, normal 45.5%, overweight 6.0%, and obesity 3.0%. The mothers of the children with cerebral palsy mainly bought milk and dairy products for their children's snacks (43.5%) as well as fruits (33.3%). They wanted development of new snacks that helped growth development (50.5%), and preferred more development of the following snacks: Korean rice cakes (47.5%), biscuits (24.2%), bread (22.3%). The result of dietary intake showed that the percentage of RI in zinc and folic acid did not reach the RI in every age categories. The proportion of subjects with less than 75% of RI was 76.8% for the zinc and folic acid, and 52.4% for the calcium and iron. These results indicate that children with cerebral palsy had slower physical development and lesser nutrition intake than normal children. Hence, this study provided the basis to develop the snack for the malnutrition state children with cerebral palsy which helped their physical development. The shape of new snack considered was a rice cake which included zinc and folic acid that were insufficient in RI in children with cerebral palsy.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.10
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• pp.1438-1445
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• 2004

The objective of this study was to determine the effects of dietary Zn level on performance, serum Zn concentrations, alkaline phosphatase activity (ALP), and immune response of pigs inoculated with Porcine Reproductive and Respiratory Syndrome virus (PRRSv) and Mycoplasma hyopneumoniae. A $2{\times}4$ factorial arrangement of treatments was used in a randomized design. Factors included; 1) PRRSv and M. hyopneumoniae inoculation (n=36 pigs) or sham inoculation (n=36 pigs) with media when pigs entered the grower facility (d 0) at 9 weeks of age and 2) 10, 50, 150 ppm supplemental Zn sulfate (${ZnSO}_4$) from weaning until the completion of the study, or 2,000 ppm supplemental ${ZnSO}_4$for two weeks in the nursery and then supplementation with 150 ppm ${ZnSO}_4$for the remainder of the trial. The basal diet contained 34 ppm Zn. Pigs were weighed on d 0, 10, 17, 24 and 31 and blood samples were collected on d 0, 7, 14, 21 and 28. Pigs inoculated with PRRSv were serologically positive at d 28 and control pigs remained negative to PRRSv. In contrast, the M hyopneumoniae inoculation was inconsistent with 33.3% and 52.8% of pigs serologically positive at d 28 in the control and infected groups, respectively. A febrile response was observed for approximately one week after inoculation with PRRSv. Feed intake (p<0.01) and gain (p<0.1) were less in PRRSv infected pigs than control pigs for the 31 d study. However, performance did not differ among pigs in the four levels of ${ZnSO}_4$. Assessments of immune responses failed to provide unequivocal influence of either PRRSv inoculation or ${ZnSO}_4$level. These data suggest that PRRSv and M. hyopneumoniae act to produce some performance deficits and the influence of Zn supplementation of nursery age pigs does not have clear effect in grower pigs affected with disease.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.3
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• pp.336-341
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• 2005

Chitosan, which is a biopolymer, composed of glucosamine units linked by β-l, 4 glycoside bonds, is rich in shells of crustacean such as crabs and shrimps. We examined effects of dietary chitosan on blood and tissue levels of lead, iron, zinc and calcium in lead administered rats. Male Sprague-Dawley rats were divided into 4 groups (n=32). Basal diet group was fed 3% cellulose diet and lead administered groups were fed 0%, 3% and 5% chitosan diets, respectively for 8 wks. To lead administered groups, lead (20㎎/day) was given three times per week by oral injection. Blood, liver, kidney and femur were collected for lead, iron, zinc, and calcium analyses. There was no significant difference in weight gain and food intake among groups. Blood and femur lead levels were lower in lead administered groups fed 3% and 5% chitosan diets than in lead administered control (0% chitosan diet) group (p＆lt;0.05). Blood and liver levels of iron and zinc in lead administered group fed 5% chitosan diet were significantly lower than those in basal diet group (p＆lt;0.05), but those in lead administered group fed 3% chitosan diet were not significantly different with those in basal diet group. These results show that chitosan diets have beneficial effects on lowering the accumulation of lead, but high chitosan diet may have negative effects on mineral levels.

• Korean Journal of Community Nutrition
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• v.10 no.5
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• pp.603-614
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• 2005

This study was performed to investigate the correlation between Premenstrual syndrome (PMS) and nutritional intake, blood composition of female students. One hundred and one female students were selected as the experimental subjects at Gyeongsang National University. To assess the PMS symptoms of the subjects, a questionnaire (PAP) score based on the methods of Halbreich, Endicor and Nee was prepared with 34 different items. Dietary survey was conducted by 24­hour recall method for 3 days and nutrient intakes were analyzed by the CAN-Pro. Blood composition of WBC, RBC, Hb, Hct, Ca and Mg was measured. The average height, weight and BMI of subjects were $160.7\pm4.6cm,\;54.1\pm5.7kg\;and\;21.2\pm1.9$, respectively. Average intakes of energy, calcium, iron, zinc, vit $B_2$, and folic acid per day were $1810.2\pm344.9kcal(90.5\%\;of\;RDA),\;621.1\;197.3mg(88.7\%\;of\;RDA),\;15.9\;pm4.9mg\;(99.3\%\;of\;RDA),\;8.1\pm3.4mg(80.5\%\;of\;RDA),\;1.1\pm0.6mg(88.3\%\;of\;RDA)\;and\;234.3\pm78.6{\mu}g(93.7\%\;of\;RDA)$, respectively. Score of the behavioral symptoms, psychologic symptoms, physical symptoms and other symptoms were recorded $1.79\pm0.86,\;2.11\pm1.08,\;2.31\pm1.11\;and\;1.58\pm0.86$, respectively. The relation between PMS and menstrual cramps was significant. A significant difference was observed for menstruation amount in physical symptoms (p<0.05) and other scores (p<0.05). The group who drinks alcohol over 30g per day showed higher scores at total PAP (p<0.05), psychologic symptoms (p<0.05), physical symptoms (p<0.05), and other scores (p<0.05) than those who don't drink alcohol. Calcium in the blood showed a negative correlation with total PAF score (p<0.05), behavioral symptoms (p<0.05), physical symptoms (p<0.05) and other scores (p<0.01). Magnesium in the blood showed a negative correlation with physical symptoms (p<0.05), other score (p<0.05). WBC was negatively associated with psychologic scores (p<0.05). Hb and Hct were negatively associated with other scores (p<0.05). In conclusion, calcium intake showed a level of $88.7\%$ of RDA and this study revealed that there is a correlation between PMS and blood composition in female college students. Therefore, calcium and magnesium supplements can be beneficial to relieve PMS symptoms.

• Culinary science and hospitality research
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• v.21 no.4
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• pp.284-293
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• 2015

This study was intended to investigate food, exercise habits, perception of health status and the effects of nutrient intake of female college students. Food habits such as regularity of meal time, repast of breakfast, numbers of night snack per week and duration of meal were assessed via a self reporting questionnaire, and 24hrs recall method for nutrient intake was obtained from 155 female college students in Yangju-si. The mean height and weight of the subjects were $161.25{\pm}3.52cm$ and $52.26{\pm}4.52kg$, respectively. The average intakes of energy and nutrients were below the Korea recommended dietary allowance(RDA). Especially, The nutrient intakes(energy, protein, fat, fiber, calcium, iron, phosphorus, zinc, sodium, potassium, vitamin B6, niacin, vitamin C, folate, vitamin E, cholesterol) of the group in which the regularity of meal time per week was 5~7 days was significantly higher than the group in which the regularity of meal time was < 3 days (p<0.005). The nutrient intakes influenced by the food habit such as the regularity of meal time, skipping breakfast, and the numbers of night snack per week but did not differ in accordance with the regular exercise habits and perception of health status. These findings suggest that nutritional education based on female college students' food habits(regularity of meal time, skipping breakfast and night snacks per week, and duration of meal) may be required to improve dietary variety. It is considered that the improvement in dietary habits will contribute to the improvement of nutrition.

• Korean Journal of Poultry Science
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• v.38 no.3
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• pp.165-171
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• 2011

This study was conducted to investigate the effects of dietary supplementation of copper-soy proteinate (Cu-SP) on the performance of laying hens. A total of 1,000 Hy-Line Brown laying hens of 32 wks old were assigned to one of the following 5 dietary treatments: Control, Cu-SP 50, 100, 150, 200 (50, 100, 150, 200 ppm Cu supplementation as Cu-soy proteinate). Each treatment was replicated 4 times with fifty birds per replication, housed in 2 birds cages. Fifty birds units were arranged according to randomized block design. Feeding trial lasted 5 wks under 16L : 8D lighting regimen. There were no significant differences among treatments in hen-day and hen-house egg production, egg weight, broken & soft egg production. Feed intake and feed conversion rate (FCR) were significantly (P<0.01) lower in Cu-SP treated groups than Control. Eggshell thickness was significantly (P<0.01) higher in Control than Cu-SP 100, 150 and 200. Egg yolk color index was significantly (P<0.01) higher in Cu-SP 200 than other treatments. Egg shell color index was significantly (P<0.05) higher in Cu-SP 150 and Cu-SP 200 than Cu-SP 100. Concentration of copper, iron and zinc of the egg yolk were not significantly influenced by treatment. There were no significant differences in the level of leukocytes and erythrocytes in the chicken blood. The result of this experiment showed that dietary supplementation of Cu-SP at the level of 50ppm of Cu can reduce feed intake and FCR.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.7
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• pp.1000-1006
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• 2015

The adolescent period, during which physical, spiritual, and physiological growth and development occurs, is important for the development of healthy dietary habits. Particularly, eating a regular breakfast improves eating habits and school life in adolescents but few studies have investigated the effects of eating breakfast on these outcomes. Therefore, this research was performed to investigate the effects of eating a regular breakfast provided by the 'Breakfast Club Program' on eating behaviors and school life. A total of 315 high school students were recruited for the program from 10 high schools in nine districts among 25 districts in Seoul during August to December of 2013. The average age was $17.8{\pm}0.8$ years and 38.1% of students were females. Based on 315 subjects, 38.3%, 35.7%, and 17.1% increased their intakes of breakfast (P=0.001), fruit (P=0.033), and milk (P<0.001) up to 5~7 times per week, respectively. The intake frequency of fruit (P<0.001) and eating breakfast (P<0.001) increased noticeably after program participation. Based on 64 students performing dietary record, intakes of cereals (P<0.001), mushrooms (P=0.027), and fruits (P=0.002) increased while intakes of eggs (P=0.001) and fat and oils (P=0.019) decreased after the program. Consumption of energy (P=0.004), plant protein (P=0.012), carbohydrates (P<0.001), dietary fiber (P=0.026), iron from plant sources (P=0.009), potassium (P=0.043), zinc (P=0.013), vitamin $B_1$ (P=0.043), and vitamin C (P<0.001) increased whereas intake of cholesterol (P=0.002) decreased. Regarding school life, the score for 'comfortable and cheerful starting of a day' (P=0.001) and 'healthy starting of a day' (P<0.001) increased significantly. This study indicates that a regular 'Breakfast Club Program' may establish healthy eating habits and school life in teenagers.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.12
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• pp.1734-1740
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• 2001

Feeding trials using weanling pigs and broiler chicks were conducted to evaluate the efficacy of different metal-amino acid chelates and complexes at various levels of copper and zinc on the performance and fecal excretions. A total of 200 weanling pigs (Large White ${\times}$ Yorkshire ${\times}$ Duroc, $11.20{\pm}0.81kg$) were randomly assigned to 5 dietary treatments following a randomized complete block design. Each treatment was replicated 4 times with 10 pigs per pen. The dietary treatments were designated as : A-diet containing 170 ppm Cu from $CuSO_4$ and 120 ppm Zn from $ZnSO_4$, B-diet containing 85 ppm Cu from Cu-amino acid chelate (CAC) and 60 ppm Zn from Zn-amino acid chelate (ZAC), C-diet containing 170 ppm Cu from CAC and 120 ppm Zn from ZAC, D-diet containing 85 ppm Cu from Cu-lysine complex (CL) and 60 ppm Zn from Zn-methionine complex (ZM), and E-diet containing 170 ppm Cu from CL and 120 ppm Zn from ZM. On the other trial, 144 of one day old broiler chicks were randomly distributed to 6 dietary treatments following a completely randomized design. Each treatment was replicated 3 times with 8 chicks per replicate. The dietary treatments were as follows: 1-diet with 60 ppm Cu from $CuSO_4$ and 40 ppm Zn from $ZnSO_4$, 2-diet with 120 ppm Cu from $CuSO_4$ and 80 ppm Zn from $ZnSO_4$, 3-diet with 60 ppm Cu from CAC and 40 ppm Zn from ZAC, 4-diet with 120 ppm Cu from CAC and 80 ppm Zn from ZAC, 5-diet with 60 ppm Cu from CL and 40 ppm Zn from ZM, and 6-diet with 120 ppm Cu from CL and 80 ppm Zn from ZM. In Exp. 1 with pigs, there was no difference on average daily gain and average daily feed intake observed among treatments. There was improvement (p<0.05) on the overall feed conversion ratio (FCR) of pigs fed diet containing 120 ppm Zn and 170 ppm Cu from metal-amino acid chelates relative to those fed diet containing inorganic sources of Cu and Zn but equally efficient as those fed diet containing metal-amino acid complexes. Pigs fed diet containing either metal-amino acid chelates or complexes as sources of Cu and Zn had higher (p<0.05) Cu and Zn concentration in serum and lower (p<0.05) in feces than those receiving diet with inorganic sources. In Exp. 2 with broiler chicks, the overall FCR was not different among treatments. Higher (p<0.05) Cu and Zn concentration in serum was obtained from birds fed diet with 60 ppm Cu and 40 ppm Zn from metal-amino acid chelates compared to those fed diet with inorganic sources of Cu and Zn. Also, the feces collected from birds fed diet with either metal-amino acid chelates or complexes contained generally lower Cu and Zn than those birds fed diet with inorganic sources. The higher the dietary level of Cu and Zn the higher the Cu and Zn concentration in the feces. Based on the results, both metal-amino acid chelates and complexes of Cu and Zn at low levels (Zn 60 ppm, Cu 85 ppm for weanling pigs and Zn 40 ppm, Cu 60 ppm for broiler chicks) are not different from that of high levels of inorganic sources in maintaining growth performance and serum concentration. The fecal excretions for Cu and Zn were greatly reduced when organic sources were used.