• Food Science and Biotechnology
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• 제18권3호
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• pp.712-716
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• 2009

The purpose of this study was to investigate the effect of dietary supplementation of sea tangle (Laminaria japonica) on the blood glucose and lipid metabolism in streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats were divided into 3 groups fed control, sea tangle powder (15%, w/w), or sea tangle water extract (4%, w/w) diet. Diabetes was induced by a single injection of STZ (60 mg/kg, i.p.) in citrate buffer. The animals were fed each of the experimental diet for 13 weeks. Serum insulin was increased by dietary supplementation of sea tangle in diabetic rats. Dietary sea tangle reduced blood glucose level of diabetic rats compared to the diabetic rats fed control diet. Dietary sea tangle also reduced the serum total cholesterol, low density lipoprotein (LDL)-cholesterol, and triglyceride in the diabetic rats. While hepatic lipids were reduced, fecal excretion of lipids was increased by supplementation with dietary sea tangle in the diabetic rats. These results indicate that dietary sea tangle decreased blood glucose and improved lipid metabolism in STZ-induced diabetic rats and this effect might be exerted by increases in serum insulin and fecal excretion of lipids.

• Journal of Nutrition and Health
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• 제30권10호
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• pp.1132-1139
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• 1997

The effect of dietary taurine supplementation on plasma and hepatic lipid concentrations were evaluated in rats fed one of the following two cholesterol-free diets for 5 weeks ; a control diet(CD : cholesterol -free and taurine -fee diet) and a taurine supplemented diet(TSD : CD + 1.5% taurine). There were no significant differences in liver weight and cummulative body weight gains between the groups at the end of the experimental period .However, the liver weight to body weight ratio was significantly decreased (p<0.05) by dietary taurine supplementation. Plasma concentrations of total cholesterol , LDL-cholesterol and triglyceride were significantly reduced(37%, 26% and 53% respectively) in rats fed TSD compared to those fed CD. There were no significant differences in plasma free fatty acid and total phospolipid levels between the two groups. Feeding TSD to the rats significantly reduced their hepatic triglyceride concentration(43% decrease , p<0.001) but elevated their hepatic free fatty acid level(77% increase, p<0.001) as compared to the control rats. Liver cholesterol concentration was not significantly influenced by the dietary taurine supplementation. Dietary taurine supplementation significantly reduced the percentage of phosphatidylcholine and phosp-atidylethanolamine, but elevated the prospholipids in the liver homogenates as compared to the values for the CD rats. These results suggest the possible roles of taurine as a hypochlesterolmic and hypotriglyceride agent in rats fed a cholesterol-free diets.

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.110-117
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• 2019

Objective: An experiment was conducted to evaluate the effects of dietary glutamine (Gln) and arginine (Arg) supplementation on performance, intestinal morphology and ascites mortality in broilers. Methods: A total of 675 day old chicks were randomly allocated to 9 experimental groups in a $3{\times}3$ factorial arrangement based on a completely randomized design with 5 replicates of 15 chicks. Three levels of dietary Gln (0%, 0.5%, and 1%) and Arg (100%, 130%, and 160% of Ross recommendation) supplementation were used in ascites inducing condition ($15^{\circ}C{\pm}1^{\circ}C$) from 7 to 42 days of age. Results: Dietary supplementation of Gln increased body weight gain during grower, finisher and total periods (p<0.05) and increased feed intake during total period. Ascites mortality was decreased by Gln supplementation (p<0.05). Gln supplementation increased the villus height (VH) and crypt depth (CD) in duodenum and jejunum (p<0.05). Arg supplementation decreased CD in duodenum and jejunum, and increased ileum villus width (VW) and also VH/CD ratio in duodenum and jejunum (p<0.05). Both Gln and Arg increased the goblet cell number (GCN) in duodenum whereas Gln supplementation decreased GCN in jejunum and ileum (p<0.05). The $Gln{\times}Arg$ interaction were observed for right ventricle (RV)/total ventricular (TV) ratio, VH, VW, CD, VH/CD. Conclusion: It was concluded that dietary 0.5% Gln alone or along with 130% Arg of Ross requirement, improve the intestinal morphology and performance and hence decrease the ascites mortality in broiler chickens with cold induced ascites.

• Asian-Australasian Journal of Animal Sciences
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• 제29권11호
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• pp.1569-1575
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• 2016

Two experiments were conducted to examine the influence of kaolinite clay supplementation (0%, 1%, or 2% diet dry matter [DM] basis) on characteristics of digestion (Trial 1) and growth performance (Trial 2) in calf-fed Holstein steers fed a finishing diet. In Trial 1, 6 Holstein steers ($539{\pm}15kg$) with ruminal and duodenal cannulas were used to evaluate treatment effects on characteristics of digestion. Kaolinite clay supplementation decreased total tract DM digestion (linear effect, p<0.01) without effects ($p{\geq}0.10$) on site and extent of digestion of organic matter, neutral detergent fiber, starch and N, or ruminal microbial efficiency. There were no treatment effects on ruminal pH, volatile fatty acids molar proportions or estimated methane production. In Trial 2, 108 Holstein steers ($132.4{\pm}5.6kg$) were used in a 308-d study to evaluate growth performance and carcass characteristics. There were no treatment effects (p>0.10) on average daily gain (ADG) and gain efficiency (ADG/dry matter intake). Kaolinite supplementation tended (linear effect, p = 0.08) to increase dietary net energy (NE) during the initial 112-d period. However, the overall (308-d) effect of supplementation dietary NE was not appreciable (p>0.20). However, due to the inertness of kaolinite, itself, the ratio of observed-to-expected dietary NE increased with kaolinite supplementation. This effect was more pronounced (linear effect, $p{\leq}0.03$) during the initial 224 d of the study. Overall (308 d), kaolinite supplementation tended to increase (linear effect, p = 0.07) dietary NE by 3% over expected. Kaolinite supplementation did not affect carcass weight, yield grade, longissimus area, kidney, pelvic and heart fat, and quality grade, but decreased (linear effect, p = 0.01) dressing percentage. It is concluded that kaolinite supplementation up to 2% of diet DM may enhance energetic efficiency of calf-fed Holstein steers in a manner independent of changes in characteristics of ruminal and total tract digestion.

• 한국가금학회지
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• 제49권1호
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• pp.9-14
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• 2022

This study was conducted to investigate the effects of dietary inclusion of phytase on the growth performance, organ weight, and tibia ash of broilers. A total of 1008 one-day-old Ross 308 broiler chicks (mixed gender) with body weight 42 ± 0.90 g (mean ± SD) were used in a trial for 32 d. Chicks were assigned to one of the two treatment diets. Each treatment consisted of 28 replicate cages, with 18 birds per cage. The dietary treatments were as follows: CON, basal diet; TRT1, basal diet + phytase 1,500 Fan Terminal Unit (FTU)/kg diet. Dietary inclusion of 1,500 FTU/kg phytase supplementation in broilers showed only slight improvements in daily feed intake (P=0.086) and feed conversion ratio (P=0.065) on day 9 compared with that in the control group. However, organ weights of the breast muscle, liver, spleen, kidney, and bursa of Fabricius were not affected by the dietary treatments. In addition, the dietary inclusion of 1,500 FTU/kg phytase supplementation in the broiler diet had no effect on tibia ash. The addition of 1,500 FTU/kg phytase in the basal diet of broilers did not have any adverse effect on growth performance, organ weight, and tibia ash, suggesting that phytase supplementation in broiler diets will exhibit comparable effects as that of corn-soybean meal-based diet.

• Asian-Australasian Journal of Animal Sciences
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• 제16권2호
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• pp.239-247
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• 2003

Two experiments were conducted with broilers to investigate the feasibility of microbial phytase replacing partial inorganic phosphorus supplementation and the synergistic effects of xylanase (320 FTU/kg) supplementation alone or in combination with phytase (750 U/kg) replacing 0.08% dietary inorganic phosphorus, on the growth performance and utilization of nutrients in broilers fed wheat-based diets. In Experiment 1, 540 broilers were fed five diets for 6 weeks. Diets C0 and C1 were corn-based diets and 0.08% inorganic P supplementation was replaced with 750 U phytase/kg feed in Diet C1. Diets W0, W1 and W2 were wheat-based diets supplemented with microbial phytase 0, 750, 750 U/kg feed and 0, 0.08% and 0.16% dietary inorganic P were replaced, respectively. In Experiment 2, 432 broilers were divided into four treatments to determine the synergistic effects of supplemental xylanase and phytase replacing 0.08% inorganic P. Four experimental diets were arranged according to a $2{\times}2$ factorial design. The results indicated that addition of phytase increased the digestibility of phytic P by 31.0 to 55%, dramatically decreased the excretion of phytic P and total P by 31.6 to 55.0% and 13.8 to 32.9%, respectively (p<0.01). It is feasible to completely replace 0.08% inorganic phosphorus supplementation with microbial phytase 750 U/kg in corn- or wheat-based diets for broilers. Addition of xylanase alone or in combination with phytase replacing 0.08% dietary inorganic P, increased body weight gain and feed utilization efficiency of broilers fed wheat-based diets (p<0.10) and decreased overall mortality (p<0.10). In the groups of birds supplementing xylanase 320 FTU/kg feed, a marked elevation of the dietary AME was observed (p<0.05). Addition of phytase replacing 0.08% dietary inorganic phosphorus, concurrently with xylanase supplementation had additive effects on the apparent digestibility of dietary phytic P and overall feed conversion ratio (p<0.05).

• 대한의생명과학회지
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• 제9권1호
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• pp.9-13
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• 2003

Currently bone biochemical markers are considered to be the best indicators of present and the future state of bone turnover. A recent study has reported that chlorella increases the bone mineral density (BMD) on postmenopausal women, but presently there are no studies on bone biochemical markers treated with chlorella dietary supplementation. The purpose of the present study was to assess the bone biochemical markers for the short term and long term treatment groups, and non-treatment group as a control. Twenty two postmenopausal woman were treated for four months and eighteen for one year with 4 gm of chlorella dietary supplementation per day, and then assessed bone biochemical markers from serum and urine samples. Bone turnover rates calculated with Osteocalcin (OC), bone specific alkaline phosphatase (BAP) as a bone formation markers and deoxypyridinoline (DP), cross-linked N-telopeptides of type I collagen (NTx) as a bone resorption markers, showed 1131$\pm$87% for control group, 61$\pm$11% for short term treated group and 190$\pm$101% for long term treated group. We conclude that chlorella dietary supplementation enhances the bone formation, and NTx as a single markers, OC/Dp as a single markers of bone turnover rate were very useful tools for determine the effectiveness of chlorella dietary supplementation (or the postmenopausal women.

• Asian-Australasian Journal of Animal Sciences
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• 제19권4호
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• pp.568-572
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• 2006

The effect of dietary lysine supplementation on the performance of crossbred lactating sows (n = 49, total 92 litters) and suckling piglets during the warm and cool seasons was studied. Sows were randomly allocated to one of two experimental diets, which were fed throughout a 28-day lactation period. Two diets were formulated based on corn-soybean meal with 6% wheat bran, containing 15% crude protein, and with or without 0.25% lysine-HCl supplementation. No interaction was observed between season and dietary lysine supplementation. Dietary lysine supplementation significantly (p<0.05) increased weaning weight (7.11 vs. 6.46 kg) and daily gain (0.20 vs. 0.18 kg) of piglets. During the warm season lactating sows had significantly lower feed intake (3.78 vs. 6.11 kg, p<0.01), higher weight loss (19.81 vs. 9.73 kg, p<0.01) and backfat loss (0.23 vs. 0.06 cm, p<0.01), longer interval from weaning to estrus (9.32 vs. 6.21 days, p<0.05), lower litter weight gain (42.13 vs. 52.90 kg, p<0.01) and lower daily gain (0.17 vs. 0.21 kg, p<0.01) than lactating sows during the cool season. The results indicate that the influences of dietary treatment and season were independent. The 15.0% CP lactation diet with 0.25% lysine-HCl supplementation did not improve the performance of lactating sows and litter piglets, which was impaired by the warm season.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.1059-1066
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• 2008

This study was carried out to investigate the effects of dietary sulfur (S) and vitamin E (Vit E) supplementation on the quality of beef from longissimus muscle of Hanwoo bulls. Eleven, 29 months-aged Hanwoo bulls were randomly divided into three feed supplementation groups (S (n = 4), Vit E (n = 4) and S+Vit E (n = 3)). S was given as much as 12 g/head/d and Vit E was given as much as 1,200 IU/head/d; both supplements were given for 3 months prior to slaughter. At 24 h post-slaughter, the carcasses were weighed and evaluated by official grader for carcass traits. At 48 h post-slaughter, the M. longissimus from each carcass was collected and stored at $4{\pm}0.2^{\circ}C$ for 10 days. There were no significant differences in yield and quality grades of carcass and proximate composition, physical properties and aroma pattern of meat among all groups. At 5 days of storage, the TBARS value of meat from cattle with S+Vit E supplementation was significantly lower (p<0.05) than other groups; and at 10 days of storage, the TBARS value of meat from cattle with Vit E and S+Vit E supplementations was significantly lower (p<0.05) than from cattle with S supplementation. At 5 days of storage, MetMb concentration of meat from cattle with S+Vit E supplementation was significantly lower (p<0.05) than from cattle with Vit E supplementation; and at 10 days of storage, MetMb concentration of meat from cattle with S+Vit E supplementation was significantly lower (p<0.05) than from other groups. At 10 days of storage, the redness value of meat from cattle with S supplementation was significantly higher (p<0.05) than from cattle with Vit E supplementation and the hue-angle value of meat from cattle with S and S+Vit E supplementations was significantly lower (p<0.05) than from cattle with Vit E supplementation. Dietary Vit E supplementation had a better effect on lipid stability whereas dietary S supplementation had a better effect on OxyMb stability. The dietary combination of S and Vit E created the highest protection for beef from myoglobin oxidation and thus improved the color stability of meat.

• Asian-Australasian Journal of Animal Sciences
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• 제13권12호
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• pp.1758-1763
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• 2000

The effect of tu-chung (Eucommia ulmoides, Oliver) leaf meal on reducing lipid accumulation in chickens fed 1% cholesterol containing diet was studied. Forty male White Leghorn chickens aged 56 days were weighed and divided into four groups of ten chickens, and fed diets with or without 1% dietary cholesterol which were supplemented with 0 and 5% tu-chung. Tu-chung supplementation to the diet without cholesterol increased acetyl-CoA carboxylase (p<0.01) but decreased 3-hydroxy-3-methylglutaryl-CoA reductase activities (p<0.01) with no effect on fatty acid synthetase activities. However, its supplementation to the diet with cholesterol had no effect on these three enzyme activities as compared with the cholesterol containing diet without tu-chung. Tu-chung supplementation to the diet without cholesterol increased hepatic triglyceride (p<0.01), whereas its supplementation to the diet with cholesterol decreased it (p<0.01). Tu-chung supplementation to the diet with cholesterol decreased plasma cholesterol ester, free cholesterol, phospholipids (p<0.05) and triglyceride (p<0.01) as compared with the cholesterol containing diet without tu-chung. Supplementation of tu-chung to the diet without cholesterol decreased plasma free cholesterol (p<0.05). It is concluded that tu-chung leaf meal reduced an increase in lipid accumulation in chickens stimulated by 1% cholesterol feeding.