• 한국가금학회지
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• 제33권3호
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• pp.239-248
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• 2006

발효가 가능한 탄수화물을 이용하거나 효소 이용 및 사료가공 등에 관한 이해를 증진시키는 길은 닭의 분중 휘발성 유가물질의 감소를 가져올 수 있다. 전분질의 소화는 가루사료를 (밀과 보리) 알곡으로 대치하게 하였다. 그러나 근위의 pH로 볼 때 사료의 종류나 형태는 연속성이 떨어진다. 펠렛으로 만든 사료는 사료 요구율이 $0\siml2%$ 증진된다. 전분질의 소화는 xylase를 첨가하였을 때 대사 에너지(ME) 가는 35% 증진 효과가 잇는 것으로 보고되었다. 전분질의 이용과 전분질이 아닌 다당류(NSP)의 이용은 전분질을 포함한 알갱이 사료의 존재 즉 비 영양소 물질(ANF)의 포함 여부에 달려 있다. 사료 생산 기술의 증가는 $33^{\circ}C$에서 만들어지는 펠렛 사료에 이용될 수 있는 건조된 상태의 효소나 액체상태의 효소 생산 기법에 달려 있다. 수용성 NSP나 arabinoxylans 또는 beta-glucan 등은 부분적으로 효소 가격이 크기로 나누어지는 정도에 따라 달라진다. 적은 크기는 수분 흡수력이 감소되어야 하는데 만약 수분 흡수력이 지나치면 소화물에 수분이 너무 많아지게 되어 분에 수분 함량이 많아지며 사료 곡물 중에 비스코시티 현상이 생겨서 계란 껍질이 지저분해진다.

• Journal of Community Nutrition
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• 제5권3호
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• pp.132-140
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• 2003

The objective of this study was to investigate the effect of soymilk and exercise on bone mineral density (BMD) in underweight college women of 19-22 years of age, who had lower bone mass. The BMD of the lumbar spine and femoral neck was measured for 52 underweight college women. Among them, 33 subjects, whose t-score value was below -1, were selected. Questionnaire survey, anthropometrical measurements, dietary recall, analysis of BMD, fasting serum osteocalcin and urinary deoxypyridinoline (DPD) were conducted before and after the 10 week study. The 33 subjects were divided into 2 groups： soymilk group (n=19), and soymilk ＋ exercise group (n=14). The soymilk group was given 400$m\ell$ soymilk containing 60mg of isoflavones on a daily basis and the soymilk ＋ exercise group exercised three times a week with a daily intake of 400$m\ell$ soymilk for 10 weeks. The average ages of the soymilk group and the soymilk ＋ exercise group were 21.1 years and 20.4 years, respectively and, there were no significant differences between the soymilk group and the soymilk ＋ exercise group in the areas of height, weight or Body Mass Index (BMI). At the baseline, the mean daily energy intake of the soymilk group and the soymilk ＋ exercise group was 1,597.9kcal (79.43％ of RDA) and 1,704.2kcal (85.2％ of RDA), respectively. The mean calcium intake of the soymilk group (408.3mg) was not significantly different from that of the soymilk ＋ exercise group (389.4mg). Despite the 400$m\ell$ soymilk supplementation, there were no significant changes of nutrient intake in either group after treatment. However, there were significant increases in BMD's of lumbar spine and femoral neck in both groups. There were some increases in the serum osteocalcin level and decreases in the urinary deoxypyridinoline level as well. BMD change of the soymilk group was not significantly different from that of the soymilk ＋ exercise group. In conclusion, supplementary intake of soymilk (containing 60mg of isoflavones) resulted in a significant increase in the BMD's of the lumbar spine and femoral neck in underweight college women with low bone mass. However, exercise did not result in any significant changes in the BMD's, implying the necessity for more intensive and specific long-term physical training for any substantial changes. Further investigation is necessary to determine the exercise that most strongly affects BMD.

• Asian-Australasian Journal of Animal Sciences
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• 제15권3호
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• pp.382-389
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• 2002

The aims of this study is to evaluate the effect of Aspergillus oryzae Fermentation Extract (AFE) on the performance of lactating cows in summer (May to July) and winter (December to February). The experiment was a completely randomized design (CRD) and dietary treatments were 1) basal diet without AFE, 2) basal plus 3 g/d AFE into the basal total mixed ration (TMR), 3) basal plus 45.4 mg AFE/kg the ensiling corn silage and 4) AFE inclusion in silage and TMR. Twenty-eight cows from each trial were selected and randomly allocated into the four treatment groups, confined in individual pens, and fed ad libitum for 8 weeks in both seasons of feeding trials. Results showed that AFE inclusion in corn silage significantly improved DM intake by 4.4% and milk yield by 3.1% (p<0.05) during summer. In the winter season, AFE inclusion in the diet significantly improved milk yield by 10%. Direct addition of AFE to the TMR even further significantly improved milk yield over the addition through corn silage by 7.4% in winter (p<0.05). An additive effect of AFE inclusion into TMR and through corn silage was also demonstrated in the winter-feeding. AFE inclusion however, did not improve DM intake during the winter trial. In the summer trial, inclusion of AFE showed an adverse effect on the percentage of milk fat, but did not impact on the milk fat yield. Adding AFE through corn silage showed a trend towards alleviating the negative effects of milk fat from direct AFE inclusion in TMR. The similar trend occurred in the winter trial. The inclusion of AFE through corn silage significantly lowered the milk protein content over direct AFE addition, but did not significantly impacted the milk protein yield in summer. AFE supplementation during the winter season significantly increased milk protein content. Adding AFE to the corn silage significantly increased milk protein content over direct AFE addition in winter although inclusion of AFE significantly decreased total milk solid content in the summer (p<0.005). During the winter season, inclusion of AFE required less DM to produce a unit of milk. Inclusion of AFE into corn silage required less DM, energy and protein to produce a unit of milk. But inclusion of AFE did not alleviate heat stress on the lactating cows.

• Asian-Australasian Journal of Animal Sciences
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• 제14권6호
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• pp.821-830
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• 2001

To study the effects of partial replacement of soybean meal (SBM) with palm kernel meal (PKM) and copra meal (CM) on growth performance, nutrient digestibility and carcass characteristics in finishing pigs, a total of 150 crossbred pigs (Landrace$\times$Duroc$\times$Yorkshire; average $52.11{\pm}1.08kg$ body weight) were alloted to five treatments, in a randomized block design. The treatments included 1) Control: without PKM or CM, 2) PKM2: 2% of palm kernel meal, 3) PKM4: 4% palm kernel meal, 4) CM2: 2% of copra meal, 5) CM4: 4% of copra meal. During the early finishing period (52~74 kg), growth performance was better in CM diets than in PKM diets or control diet, and in overall period (74~100 was lower (p<0.05) in PKM4 diet than the other diets. Nutrient digestibilies of PKM or CM substituted diets showed the tendency to be lower than those of control diet. In the early finishing period, total amino acid digestibilities of PKM and CM diets had the tendency to be lower than control diet, and in the late finishing period, they were lower (p<0.05) than control diet. Carcass length was longer (p<0.05) in the pigs fed 2% CM than in the pigs fed 4% PKM diet, but other carcass characteristics were not different among treatments. Although the dietary C14:0 content affected (p<0.05) on the C14:0 content in the carcass, the inclusion of PKM or CM in the diet did not affect the total saturated fatty acids and unsaturated fatty acids in the backfat of finishing pigs. Although it was not significant, supplementation of CM at the 2% and 4% of control group tended to decrease feed cost per kg weight gain by 2.89 to 1.42%, respectively. In conclusion, copra meal can be a valuable source of protein in the diet for finishing pigs and may replace other protein sources in pig diets to a considerable extent.

• Journal of Animal Science and Technology
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• 제61권6호
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• pp.313-323
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• 2019

The interactive effect of dietary fat supplementation and milk yield level on dairy cows performance under heat stress has not been thoroughly investigated. The purpose of this study was to evaluate the effect of production level, the source of fat supplements and their interaction on dairy cows performance under heat stress. In this study, 64 Holstein multiparous cows were divided into 2 groups and received one of two rations having either calcium salts of fatty acids (Ca-FA) or high-palmitic acid (PA) supplements (2.8% of DM; dry matter). After completing the experiment and based on maturity-equivalent milk, cows were divided into two groups of high-yielding (14,633 kg) and medium-yielding (11,616 kg). Average temperature humidity index (THI) was 71 during the trial period. Apparent digestibility of dry matter (p = 0.04), organic matter (p = 0.05), and neutral detergent fiber (NDF; p = 0.04) for cows fed Ca-FA were greater than cows fed PA. The milk fat content in high-producing cows was 0.3% greater than medium-producing cows (p = 0.03). The milk protein content in cows fed Ca-FA was greater than cows fed PA (p < 0.01). High-producing cows had greater serum cholesterol (p = 0.02) than medium-producing cows. The cows fed PA tended to have a greater BUN than cows fed Ca-FA (p = 0.06). Alanine aminotransferase and aspartate aminotransferase tended to be increased by PA, which indicates that cows in PA treatment may have experienced more adverse effect on the liver function than cows on Ca-FA. Therefore, under heat stress and in 90 d trial, milk production level does not affect the cows' response to PA or Ca-FA. Although cows fed Ca-FA received lower energy than those fed PA, they compensated for this shortage likely with increasing the digestibility and produced a similar amount of milk.

• Nutrition Research and Practice
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• 제8권2호
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• pp.158-164
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• 2014

BACKGROUD/OBEJECTIVES: This study aims to find out the effects of peanut sprout extracts on weight controls and protein expressions of transcription factors related to adipocyte differentiation and adipocytokine in rats under high-fat diets. MATERIALS/METHODS: Four week-old Sparague-Dawley (SD) were assigned to 4 groups; normal-fat (NF) diets (7% fat diet), high-fat (HF) diets (20% fat diet), high fat diets with low peanut sprout extract (HF + PSEL) diet (20% fat and 0.025% peanut sprout extract), and high fat diets with high peanut sprout extract (HF + PSEH) diet (20% fat and 0.05% peanut sprout extract). Body weight changes, lipid profiles in adipose tissue, and the mRNA protein expressions, such as peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$), CCAAT element binding protein ${\alpha}$ (C/EBP ${\alpha}$), leptin, and adiponectin, were determined. RESULTS: After 9 weeks of feeding, the HF + PSEH group had significantly less weight gains than the HF group (P < 0.05). However, the total dietary intakes or food efficiency ratios among groups were not significantly different. The weight of epididymal fat in HF + PSEH group, $3.61{\pm}0.5g$, or HF + PSEL group, $3.80{\pm}0.7g$, was significantly lower than the HF group, $4.39{\pm}0.4g$, (P < 0.05). Total lipids and total cholesterol in adipose tissue were significantly decreased in HF + PSEH group compared to those in the HF group, respectively (P < 0.05). PSEH supplementation caused AST and ALT levels to decrease when it compared to HF group, but it was not statistically significant. The protein expression of $PPAR{\gamma}$ in HF + PSEH group was significantly lower than the HF group (P < 0.05). Comparing with the HF group, the protein expression of adiponectin in HF + PSEH group was significantly increased (P < 0.05). The protein expressions of C/EBP ${\alpha}$ and leptin in HF + PSEH group were lower than the HF group, but it was not statistical significant. CONCLUSIONS: In conclusion, peanut sprout extract has anti-obesity effect by lowering the expressions of $PPAR{\gamma}$ which regulates the expression of adiponectin.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.976-982
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• 2007

This study investigated whether supplemental Cu, Fe, Zn, and Mn are needed in a practical diet for pullets. Four hundred and twenty females of an egg-laying strain (1-d-old, Lohmann Brown Layer) were randomly distributed into 4 groups, consisting of 7 replicates of 15 birds each. During the 18-week experimental period, chicks were given three basal diets in sequence, each with single or multiple Mn, Zn and Cu supplementation to improve the mineral balance gradually. In the Control, no Mn, Zn, and Cu were added; in the single Mn supplemented group (sMn) Mn was added to 120, 60, and 60 mg/kg for 1-6, 7-12, and 13-18 weeks of age, respectively; in the multiple Mn and Zn supplemented group (mMnZn), Mn was added to 180, 90, and 90 mg/kg and Zn was added to 120, 105, and 105 mg/kg for 1-6, 7-12, and 13-18 weeks of age, respectively; in the multiple Mn, Zn, Cu supplemented group (mMnZnCu), Mn, Zn, and Cu were added to the same multiple of basal Fe concentration relative to NRC (1994) recommendations. Energy and protein metabolizability were determined by subtracting energy/protein intake by energy/protein excretion (from both feces and urine) and dividing by energy/protein intake. There were no statistically significant differences between groups in terms of feed intake, final body weight or tibia length throughout the experiment. Optimal growth performance was observed in the Control, while adding trace minerals to basal diets tended to result in decreased productive performance. Protein metabolizability was increased by mMnZn and mMnZnCu treatments, but energy metabolizability was not affected. Concentrations of Mn, Zn, Cu in excreta varied greatly related to dietary content, and the retentions of Cu, Fe, Zn and Mn were all increased due to the improvement of mineral balance. Based on these results, it is suggested that the concentrations of Cu, Fe, Zn and Mn in typical basal diets used in this study were adequate for normal growth for pullets from 1 to 18 weeks of age.

• Journal of Microbiology and Biotechnology
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• 제29권11호
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• pp.1693-1706
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• 2019

Atopic dermatitis (AD) is a chronic inflammatory skin disease of mainly infants and children. Currently, the development of safe and effective treatments for AD is urgently required. The present study was conducted to investigate the immunomodulatory effects of yeast-extracted β-1,3/1,6-glucan and/or Lactobacillus plantarum (L. plantarum) LM1004 against AD-like symptoms. To purpose, β-1,3/1,6-glucan and/or L. plantarum LM1004 were orally administered to AD-induced animal models of rat (histamine-induced vasodilation) and mouse (pruritus and contact dermatitis) exhibiting different symptoms of AD. We then investigated the treatment effects on AD-like symptoms, gene expression of immune-related factors, and gut microbiomes. Oral administration of β-1,3/1,6-glucan (0.01 g/kg initial body weight) and/or 2 × 10¹² cells/g L. plantarum LM1004 (0.01 g/kg initial body weight) to AD-induced animal models showed significantly reduced vasodilation in the rat model, and pruritus, edema, and serum histamine in the mouse models (p < 0.05). Interestingly, β-1,3/1,6-glucan and/or L. plantarum LM1004 significantly decreased the mRNA levels of Th2 and Th17 cell transcription factors, while the transcription factors of Th1 and Treg cells, galactin-9, filaggrin increased, which are indicative of enhanced immunomodulation (p < 0.05). Moreover, in rats with no AD induction, the same treatments significantly increased the relative abundance of phylum Bacteroidetes and the genus Bacteroides. Furthermore, bacterial taxa associated with butyrate production such as, Lachnospiraceae and Ruminococcaceae at family, and Roseburia at genus level were increased in the treated groups. These findings suggest that the dietary supplementation of β-1,3/1,6-glucan and/or L. plantarum LM1004 has a great potential for treatment of AD as well as obesity in humans through mechanisms that might involve modulation of host immune systems and gut microbiota.

• 한국식품저장유통학회지
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• 제16권2호
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• pp.238-245
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• 2009

본 연구에서는 천일염의 아직 밝혀지지 않은 기능성과 청국장의 효과를 감안하여 천일염으로 제조된 청국장의 기능적 우수성을 평가하기 위하여 Bacillus subtilis DJI 균주를 접종하여 발효시킨 무염청국장, 3% 천일염 첨가 청국장, 3% 정제염 첨가 청국장과 3% 정제염이 첨가되어진 시판청국장의 에탄올, 물 및 헥산 추출물의 항산화력을 비교 검토하였다. 청국장 에탄올, 헥산 및 물 추출 수율은 각각 $10.9{\sim}11.8%$, $15.7{\sim}16.9%$, $18.6{\sim}19.4%$ 범위로 청국장 용매별 간에는 서로 유사한 경향이었으며, 물 추출 수율이 가장 높게 나타났다. 추출물의 DPPH에 대한 전자소거능, 아질산염 소거능, linoleic acid에 대한 과산화물가 및 항산화지수 측정에 의한 항산화효과는 에탄올 추출 시료군이 물 추출 시료군에 비하여 높았다. 청국장 추출물의 전자소거능과 아질산염 소거능은 무염청국장과 3% 천일염 첨가 청국장 추출물이 가장 우수하였으나, 합성항산화제에 비해서는 낮은 효능을 보였다. 청국장 추출물 중 항산화지수는 3% 천일염 첨가 청국장 추출물이 가장 우수하였다. Bacillus subtilis DJI 균주를 접종하여 발효시킨 청국장인 무염청국장, 3% 천일염 첨가 청국장 및 3% 정제염 첨가 청국장이 시판청국장에 비하여 항산화효과가 우수하였으며, 균주를 접종하여 발효시킨 청국장 중 무염청국장과 3% 천일염 첨가 청국장이 3% 정제염 첨가 청국장에 비하여 항산화효과가 다소 우수하였다.

• 한국식품영양과학회지
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• 제25권6호
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• pp.963-968
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• 1996

20% 생콩가루 및 생콩가루에 마그네슘 첨가 식이로 streptozotoxin 유발 당뇨쥐를 4주간 사육하여 체중, 간과 췌장무게, 혈당, 혈중 지질 농도 및 간의 지질 농도에 미치는 영향을 조사하였다. 모든 당뇨군은 당뇨병으로 인한 대사장애로 정상군에 비하여 체중이 감소되었다. 간의 총 무게는 대조군과 모든 당뇨군이 비슷한 무게를 보였으나, 체중에 대한 간 무게의 %비율은 모든 당뇨군이 대조군 보다 유의하게 증가하였다. 췌장의 무게는 대조군 및 당뇨대조군에 비하여 당뇨생콩군 및 당뇨생콩+마그네슘군이 유의하게 증가되었다. 혈당량은 당뇨대조군에 비하여 당뇨생콩군 및 당뇨생콩+마그네슘군이 각각 73% 및 44% 유의하게 감소되었으나, 정상대조군에 비하여는 당뇨생콩군 및 당뇨생콩+마그네슘군이 각각 77및 111%증가되어 정상수준으로 혈당을 낮추지는 못하였다. 혈청의 중성지질, 인지질 및 총 콜레스테를 농도는 당뇨생콩군과 당뇨생콩+마그네슘군이 당뇨대조군에 비하연 유의하게 감소되어 정상대조군과 비슷한 경향으로 혈청의 지질 농도는 정상 수준으로 개선되었다. 간의 총 지질과 중성지질 농도는 대조군에 비하여 각 당뇨군이 유의하게 감소 되었으며, 특히 당뇨생콩+마그네슘군이 가장 낮은 농도를 보였다. 인지질 농도는 당뇨생콩+마그네슘군이 유의하게 감소되었다. 그리고 간의 총 콜레스테를 농도는 각 실험군이 변화를 보이지 않았다. 혈청의 HDL-콜레스데를 농도는 대조군과 당뇨실철군이 비슷한 수준을 보였다. 이상의 결과로 보아 생콩이 당뇨쥐의 혈당을 낮추어 주는 작용이 있고, 혈청 지질대사의 개선효과 등 부분적인 개선 효과가 있는 것으로 나타났으나 앞으로 많은 연구가 이루어져야 할 것으로 사료된다.