• Animal Bioscience
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• v.34 no.4
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• pp.594-602
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• 2021

Objective: An experiment was conducted to assess the effect of dragon fruit peel pellet (DFPP) as a rumen enhancer of dry matter consumption, nutrient digestibilities, ruminal ecology, microbial protein synthesis and rumimal methane production in Holstein crossbred bulls. Methods: Four animals, with an average live-weight of 200±20 kg were randomly assigned in a 4×4 Latin square design to investigate the influence of DFPP supplementation. There were four different dietary treatments: without DFPP, and with 200, 300, and 400 g/h/d, respectively. Results: Results revealed that dry matter consumption of total intake, rice straw and concentrate were not significantly different among treatments (p>0.05). It was also found that ruminal pH was not different among treatments (p>0.05), whilst protozoal group was reduced when DFPP increased (p<0.01). Blood urea nitrogen and NH3-N concentrations were increased at 400 g of DFPP supplementation (p<0.01). Additionally, volatile fatty acid production of propionate was significantly enhanced by the DFPP supplementation (p<0.05), while production of methane was consequently decreased (p<0.05). Furthermore, microbial protein synthesis and urinary purine derivatives were remarkably increased especially at 400 g of DFPP supplementation (p<0.05). Conclusion: Plant secondary compounds or phytonutrients (PTN) containing saponins (SP) and condensed tannins (CT) have been reported to influence rumen fermentation. DFPP contains both CT and SP as a PTN. The addition of 400 g of DFPP resulted in improved rumen fermentation end-products especially propionate (C3) and microbial protein synthesis. Therefore, DFPP is a promising rumen enhancer and indicated a significant potential of DFPP as feedstuff for ruminant feed to mitigate rumen methane production.

• Food Science of Animal Resources
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• v.41 no.1
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• pp.45-58
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• 2021

This study investigated the dietary effect of total mixed ration (TMR) based on high roughage content on the growth performance, carcass characteristics, and meat quality of Hanwoo steers. Twenty-four Hanwoo steers (average body weight, 195.3±4.7 kg; age, 8.5 mon) were randomly allocated to three experimental groups according to forage and concentrate ratio (DM basis): 25:75 (control), 50:50 (T50), and 70:30 (T70). Productivity in the fattening period and final body weight were significantly higher in the control. Average daily gain and feed conversion ratio were the same among treatments. Serum parameters, cholesterol, blood urea nitrogen, and total protein were higher in the control. Carcass weight was comparable in the control and T50 but feeding more roughage was significantly correlated with a higher intramuscular fat. Shear strength and drip loss were higher while n-6/n-3 was lower in T70 compared to the other groups. However, meat color was not significantly different among treatments. In terms of free amino acid contents, glutamic acid and glycine were higher in the control than T50 and T70. Overall, feeding Hanwoo steers with high forage content TMR had the lowest n-6/n-3 ratio of fatty acid content but highest intramuscular fat, shear strength, and drip loss. High forage content TMR is the best feed for Hanwoo steers that gives more benefits for human health and consumption but also provides the best meat grade and quality, which is important in the beef market in Korea.

• Food Science of Animal Resources
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• v.42 no.3
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• pp.517-535
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• 2022

The primary aim of this study was to evaluate the effect of feeding alfalfa: Concentrate at different ratios (8:2 or 2:8) to Korean native black goats (KNBG) for 90 days on meat quality and bioactive compound content. Feeding KNBG alfalfa and concentrate at different ratios did not impact meat pH, color, microorganism composition, volatile basic nitrogen levels, or lipid oxidation. The low alfalfa (KLA) group exhibited increased oleic acid and monosaturated fatty acid levels, both of which impact the palatability traits of meat. The abundance of bioactive compounds increased in the loin meat of the KLA group, leading to an increase in antioxidant activities. Our results suggest that feeding alfalfa and concentrate at a 2:8 ratio to KNBG can increase taste-related fatty acids and bioactive compounds in loin meat, relative to that achieved by feeding at an 8:2 ratio. Further investigation is required to evaluate the quality and the metabolites of bioactive compounds in KNBG meat and the effect of the different dietary ratios of forage and concentrate.

• Journal of Animal Science and Technology
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• v.64 no.4
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• pp.603-620
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• 2022

Human activities have caused an increase in greenhouse gas emissions, resulting in climate change that affects many factors of human life including its effect on water and food quality in certain areas with implications for human health. CH₄ and N₂O are known as potent non-CO₂ GHGs. The livestock industry contributes to direct emissions of CH₄ (38.24%) and N₂O (6.70%) through enteric fermentation and manure treatment, as well as indirect N₂O emissions via NH₃ volatilization. NH₃ is also a secondary precursor of particulate matter. Several approaches have been proposed to address this issue, including dietary management, manure treatment, and the possibility of inhibitor usage. Inhibitors, including urease and nitrification inhibitors, are widely used in agricultural fields. The use of urease and nitrification inhibitors is known to be effective in reducing nitrogen loss from agricultural soil in the form of NH₃ and N₂O and can further reduce CH₄ as a side effect. However, the effectiveness of inhibitors in livestock manure systems has not yet been explored. This review discusses the potential of inhibitor usage, specifically of N-(n-butyl) thiophosphoric triamide, dicyandiamide, and 3,4-dimethylpyrazole phosphate, to reduce emissions from livestock manure. This review focuses on the application of inhibitors to manure, as well as the association of these inhibitors with health, toxicity, and economic benefits.

• Journal of Animal Science and Technology
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• v.64 no.4
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• pp.740-751
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• 2022

Two experiments were conducted to determine the effects of bird age on apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) for 10-d-old Experiment (Exp. 1) and 22-d-old (Exp. 2) male broilers. This study investigated the effects of different broiler ages and feed ingredients on AID and SID of AA in corn and soybean meal (SBM). Four hundred and eighty (age = 7 d; initial body weight [BW] = 173.4 ± 12.65 g) and 192 (age = 18 d; initial BW = 772.2 ± 62.13 g) birds were allocated to three dietary treatments in a randomized complete block design with eight replicate cages per treatment. Two diets were formulated based on corn or SBM as the sole source of AA in the diet. A nitrogen-free diet was also formulated to measure basal endogenous losses of AA. Experimental diets were given for 3 and 4 days in Exps. 1 and 2, respectively. An interaction was observed (p < 0.05) between the age of birds and the type of ingredient for the AID of most AA, except for methionine, valine, cysteine (Cys), and tyrosine; however, the effects of age and type of ingredients were diminished in the SID of AA, except for histidine, isoleucine, leucine (Leu), phenylalanine, alanine (Ala), and glutamic acid (Glu). The AID of AA, except for Leu and Cys and the SID of AA, except for Leu, Ala, Glu, and Pro in SBM were greater (p < 0.05) than in corn. As the age of birds increased from 10 to 22 d, digestibility of all AA increased (p < 0.05), regardless of the expression of AA digestibility (i.e., AID and SID). In conclusion, the AID and SID of AA in both corn and SBM increased with increasing age, and the AID and SID of AA in SBM were greater than in corn.

• Animal Bioscience
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• v.36 no.11
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• pp.1647-1654
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• 2023

Objective: Aging roosters typically exhibit subfertility with decreasing semen quality, furthermore Thai native roosters reared in rural areas are raised for a longer duration than their usual lifespan. The present study therefore aimed to assess the effect of selenium supplementation as an antioxidative substance in diets to improve the semen cryopreservation of aged roosters. Methods: Semen samples were collected from young (n = 20) and aged (n = 20) Thai native roosters (Pradu Hang Dum) at 36 and 105 weeks of age when starting the experiment, respectively. They were fed diets either non-supplemented or supplemented with selenium (0.75 ppm). Fresh semen quality and lipid peroxidation of fresh semen was evaluated before cryopreservation using the traditional liquid nitrogen vapor method. Post-thaw sperm quality and fertility potential were determined. Results: Advancing age is unrelated to decreasing fresh semen quality (p>0.05). However, lipid peroxidation in rooster semen depended on age, and the malondialdehyde (MDA) concentration increased in aged roosters (p<0.05). Selenium supplementation in diets significantly decreased the MDA concentration and increased the sperm concentration (p<0.05). In contrast, cryopreserved semen was affected by advancing rooster age, and selenium influenced sperm quality (p<0.05). Younger roosters had higher post-thaw sperm quality and fertility potential than aged roosters (p<0.05). Likewise, diet selenium supplements improved post-thaw sperm quality and fertility compared with the non-supplement group. Conclusion: Rooster's age does not influence the rooster sperm quality of fresh semen, while sperm cryotolerance and fertility were greater in young roosters than in aged roosters. However, sperm of aged roosters could be improved by dietary selenium supplementation.

• Journal of Animal Science and Technology
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• v.48 no.6
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• pp.819-826
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• 2006

The objective of the present study was to investigate the effect of dietary supplementation of cultured wild-ginseng powder or its fermented culture byproduct on growth performance, blood parameters, carcass and meat quality in finishing pigs. The animals used in the experiment were a total of 36 Landrace×Yorkshire and weighted 65.81±2.02kg. The experimental diets were basis diet, 2.5% wild-ginseng fermented culture byproduct of B. subtilis replaced lupin in basis diet and 0.2% cultured wild-ginseng powder replaced lupin in basis diet to CON, T1 and T2 for 60 days, respectively. The pigs were allotted at 4 pigs per pen with three replicate pens per treatment by completely randomized design. In growth performance, ADG was not significantly different between treatments. ADFI was significantly lower (P<0.05) in T1 and T2 than in CON. Feed/Gain was not different between treatments. In plasma's biochemical composition, total protein was significantly higher (P<0.05) in T1 than in CON. Blood urea nitrogen was not different between treatments. Glucose and albumin were significantly higher (P<0.05) in T1 than in other treatments. Calcium was significantly higher (P<0.05) in T1 than in CON. Inorganic phosphate was significantly higher in T1 than in other treatments. In plasma's lipid composition, triglyceride was significantly higher (P<0.05) in T1 than in other treatments. Total cholesterol was not different between treatments. HDL cholesterol was significantly higher (P<0.05) in T1 than in other treatments. In carcass and meat quality, carcass weight, dressing precent, meat precent and back-fat thickness were not significantly different between treatments. Moisture and crude fat were also not significantly different between treatments. The results indicate that growth performance, carcass and meat quality were not affected but plasma's biochemical and/or lipid composition were affected when replaced with wild-ginseng fermented culture byproduct of B. subtilis and cultured wild-ginseng. Our research indicates that wild-ginseng fermented culture byproduct of B. subtilis and cultured wild-ginseng powder were able to using with pig's diet in finishing period.

• Journal of Animal Science and Technology
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• v.49 no.6
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• pp.839-846
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• 2007

The objective of the present study was to investigate the effect of dietary supplementation of the ginseng by-product on growth performance and meat quality in finishing pigs. The animals used in the experiment were a total of 24 Landrace×Yorkshire and weighted 65.81±2.02kg. The experimental diets were basal diet(CON) and 2.5% ginseng by-product(GBP), which replaced lupin in basal diet. The pigs were allotted at 4 pigs per pen with three replicate pens per treatment by completely randomized design. In growth performance, ADFI was significantly lower(P<0.0001) in GBP than in CON. In plasma biochemical composition, total protein(P<0.01), blood urea nitrogen(P<0.03), glucose(P<0.01), albumin(P<0.02), calcium(P<0.01) and inorganic phosphate(P<0.01) were significantly higher in GBP than in CON. Carcass and meat quality were not significantly different between treatments. Total ginsenoside content on meat was significantly higher(P<0.0001) in GBP than in CON. TBARs was significantly lower in GBP than in CON for 6 days(P<0.03) and 12 days (P<0.06), respectively. Our research indicates that plasma biochemical composition, total ginsenoside content and TBARs were affected when replaced with ginseng by-product. Ginseng by-product in the pig diet increased pig muscle ginsenoside, indicating that ginseng by-product can be used as a feed additive.

• Journal of Animal Science and Technology
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• v.49 no.6
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• pp.745-752
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• 2007

A total of thirty sows(Landrace×Yorkshire) were used to determine the effects of plant protein source containing multienzyme on performance, nutrients digestibility and milk characteristics. A feeding trial was conducted for 21 days from parturition to weaning. Experimental diets were supplied for 1 week before the parturition day and throughout the experimental period. Dietary treatments included: 1) Control(CON; basal diet), 2) CGLT(included corn gluten) and 3) FSPM(included fermented soy protein containing multienzyme). Through the entire experimental period, backfat loss and return-to-estrus intervals were not affected by the treatments(P>0.05). Nitrogen digestibility was increased significantly(P<0.05) in FSPM treatment compared to CON treatment. Blood urea nitrogen(BUN) concentration was increased significantly (P<0.05) in FSPM treatment compared to CON treatment. At the initial  period, total protein content of milk was higher significantly(P<0.05) in FSPM treatment compared to CGLT treatment and at the final period, total fat content of milk was higher significantly(P<0.05) in FM treatment compared to CON treatment. Rectal temperature showed similar tendency of change among treatments. The final piglet body weight, weight gain and ADG were higher significantly in FSPM treatment compared to CON treatment. On diarrhea rate in piglet, just one piglet occurred in CGLT treatement. In conclusion, 2.5% dietary plant protein source containing multienzyme suppelmentation improved N digestibility, BUN concentration, fat and protein contents in milk and weight gain in piglet.

• Journal of Animal Science and Technology
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• v.49 no.4
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• pp.501-508
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• 2007

This study was conducted to evaluate effect of probiotic complex(Lactobacillus acidophilus, Bacillus subtilis and Aspergillus oryzae) on growth performance, blood immunological parameters and fecal malodor gas emissions in growing pigs. Forty-eight pigs[(Landrace × Yorkshire) × Duroc, 25.31±1.29kg average initial body weight] were used in 35d growth trial. Dietary treatments included CON(basal diet), PC1(basal diet + 0.1% probiotic complex) and PC2(basal diet + 0.2% probiotic complex). From d 0 to 20, ADFI was significantly increased in PC1 and PC2 compared to CON(Linear effect, P=0.013). From d 21 to 35, ADFI was increased in PC1 compared to CON(Quadratic effect, P=0.024). For the whole period, ADFI was increased PC2 and PC1 compared to CON(Linear effect, P=0.009, Quadratic effect, P=0.004). For the whole period, ADG was increased in PC1 compared to CON(Quadratic effect, P=0.017). G/F was not affected by treatments. Dry matter digestibility in PC2 was higher than PC1 and CON(Linear effect, P=0.001). Nitrogen digestibility was significantly higher in PC2 and PC1 than CON(Linear effect, P=0.005). In blood immunological parameters, Total protein, IgG, red blood cell(RBC) and white blood cell(WBC) were increased in PC2 compared to PC1 and CON(Linear effect, P<0.001, Quadratic effect, P<0.001). In fecal malodor gas emission, ammonia and acetic acid were significantly reduced in PC2 compared to CON(Linear effect, P<0.02). Hydrogen sulfide was significantly reduced in PC2 compared to CON(Linear effect, P=0.0002, Quadratic effect, P=0.018). However, total mercaptans was not affected by treatments. Water content of feces was not significantly different among the treatments. In conclusion, 0.2 % probiotic complex improved ADFI, apparent dry matter and nitrogen digestibility, Total protein, IgG, RBC and WBC. Also, it decreased ammonia, acetic acid and hydrogen sulfide emissions in growing pigs.