• 한국축산식품학회지
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• 제33권3호
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• pp.341-347
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• 2013

This study was conducted to evaluate the effect of early diet dilution with 40% rice hull on growth performance, carcass characteristic and composition of meat-type ducks, and to reveal the possible mechanism for decreased body fat deposition. 160 1-day-old White Peking ducks with initial body weight of $44.5{\pm}1.0$ g were allotted to two treatments with 8 replicate pens per treatment and 10 ducks per pen (5 male and 5 female). Ducks were fed with the experimental starter diets diluted with 0% (control, RH0), 40% rice hull (RH40) during 8 to 14 d of age, respectively. Thereafter, all ducks were fed with grower diet. Ducks fed with RH40 diet from 8 to 14 d of age increased (p<0.05) feed intake, decreased (p<0.05) body weight, body weight gain and adjusted feed intake (excluded rice hull), abdominal fat, skin with fat, and fat content in carcass, and reduced (p<0.05) activities of hepatic malic dehydrogenase, glucose-6-phosphate dehydrogenase and fatty acid synthetase. When diet dilution was withdrawn in the re-fed period from 15 to 42 d of age, full compensatory growth of body weight, breast meat and leg meat weight were attained. However, ducks were still less (p<0.05) carcass fat content and showed continually lower (p<0.05) hepatic lipogenic enzyme activities at the market age in RH40 ducks than the control. These results indicated that diluting diet with 40% rice hull during 8 to 14 d of age might be a suitable method to improve feed efficiency, and to reduce carcass fat deposition in the production of meat-type ducks.

• Asian-Australasian Journal of Animal Sciences
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• 제29권4호
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• pp.523-529
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• 2016

An isotope dilution method using $[U-^{13}C]glucose$ and $[1-^{13}C]leucine$ (Leu) was conducted to evaluate the effects of rice straw supplemented with urea and molasses (RSUM-diet) on plasma glucose and Leu turnover rates in sheep. Nitrogen (N) balance, rumen fermentation characteristics and blood metabolite concentrations were also determined. Four sheep were fed either mixed hay (MH-diet), or a RSUM-diet with a crossover design for two 21 days period. Feed allowance was computed on the basis of metabolizable energy at maintenance level. The isotope dilution method was performed as the primed-continuous infusion on day 21 of each dietary period. Nitrogen intake was lower (p = 0.01) for the RSUM-diet and N digestibility did not differ (p = 0.57) between diets. Concentrations of rumen total volatile fatty acids tended to be higher (p = 0.09) for the RSUM-diet than the MH-diet. Acetate concentration in the rumen did not differ (p = 0.38) between diets, whereas propionate concentration was higher (p = 0.01) for the RSUM-diet compared to the MH-diet. Turnover rates as well as concentrations of plasma glucose and Leu did not differ between diets. It can be concluded that kinetics of plasma glucose and Leu metabolism were comparable between the RSUM-diet and the MH-diet, and rumen fermentation characteristics were improved in sheep fed the RSUM-diet compared to the MH-diet.

• 한국가금학회지
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• 제29권1호
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• pp.37-43
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• 2002

본 시험은 왕겨에 의한 사료의 희석과 무급여일 설정방법에 의한 조기제한사양이 육계의 보상성장과 사료효율 및 체지방 축적에 미치는 영향을 구명하기 위해 7일령된 무감별 육계 300수를 5처리 3반복, 반복당 20수씩 공시하여 7일령에서부터 49일령까지 실시하였다. 대조구(C)는 7일령부터 49일령까지 전기간 육계 전기사료와 후기사료를 자유채식을 시켰으며, 시험구(71-74)들은 모두 7일령부터 14일령까지 육계전기사료와 왕겨를 50:50(wt/wt)으로 혼합한 희석사료를 급여하였으며, 제한사양기간동안 T1은 희석사료를 자유채식시켰으며, T2는 1일절식-3일급여, T3는 1일절식∼2일급여 및 T4는 1일절식∼1일급여하였고, 제한 사양이 끝난 시험구들은 전기사료 및 후기사료를 자유채식시켰다. 제한사양기간을 포함한 21일령까지의 증체량과 21일령 체중 모두 T1은 대조구와 유의적 차이가 없었으나 다른 시험구들은 대조구보다 적었다(P<0.05). 22일령부터 49일령가지의 증체량은 T1구가 대조구와 유의적인 차이는 없었으나 가장 높은 증체를 보였다. 전기간인 7일령부터 49일령 까지의 증체량과 49일령 체중도 T1구만 대조구와 유의적인 차이가 없었고 다른 시험구들은 대조구보다 적었다(P<0.05) .제한사양후부터 21일령까지의 사료섭취량은 T1구를 제외한 나머지 시험구에서는 대조구에 비해 유의적으로 적었으며 (P<0.05) , 전기간 셥취량도 T1구만 대조구와 유의적인 차이를 보이지 않았으나, 희석사료섭취기간의 왕겨섭취량을 제외한 육계사료 섭취량에서는 모든 시험구들이 대조구에 비해 유의적으로 적게 섭취하였다(P<0.05) 제한사양기간을 포함한 7일령부터 21일령까지의 사료요구율은 T1구와 T2구가 대조구에 비해 유의적으로 높았으며(P<0.05), 22일령부터 49일령가지의 사료요구율은 대조구에 비해 모든 시험구들이 유의적으로 낮았으며(P<0.05), 전기간동안의 사료요구율은 T1구를 제외한 나머지 시험구들은 대조구에 비해서 유의적으로 낮았으나(P<0.05) , 희석사료섭취기간의 왕겨섭취 량을 제외 한 육계 사료요구율은 대조구에 비해서 모든 시험구들이 유의적으로 낮거나(P<0. 05) 낮아지는 경향을 보였다. 복강내 지방함량 및 전기간 폐사율은 처리간에 유의적 차이가 없었다.

• Asian-Australasian Journal of Animal Sciences
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• 제5권1호
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• pp.75-79
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• 1992

Fourteen Sahiwal ${\times}$ Friesian crossbred heifers were used in a 10-wk feeding trial to determine maintenance energy requirements and efficiency of gain. The heifers were individually fed with a diet consisting of 30% dry grass and 70% concentrates at either 110, 140 or 180% of the anticipated maintenance requirement ($494kJ\;ME/kg^{0.75}/day$). Liveweight of individual heifers was measured weekly to calculate diet requirements and average daily gain (ADG). Diet digestibility was determined for all heifers to determine ME intake. Retained energy (RE) of individual heifers was determined from changes in total body fat and protein using a TOH isotope dilution procedure and, assuming calorific values of 39.3 and 23.6 kJ/g for fat and protein respectively. The estimated ME for maintenance was 433 and $470kJ/kg^{0.75}/day$ by liveweight (ADG) equilibrium and energy (RE) equilibrium analysis respectively. ME requirement for one g of liveight gain was 28 kJ.

• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1333-1339
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• 2010

The present study was conducted to determine plasma acetate, glucose and protein metabolism using dilution of isotopes [[1-$^{13}C$]Na acetate, [U-$^{13}C$]glucose and [1-$^{13}C$]leucine (Leu)] in sheep fed rice straw (Oriza japonica L.). Four sheep were assigned to either rice straw (RS-diet) or mixed hay (MH-diet) with a crossover design. Nitrogen (N) intake and N digestibility were lower (p = 0.002 and p = 0.02, respectively) for RS-diet than MH-diet, but N retention did not differ (p>0.10) between the diets. Concentrations of rumen acetate tended to be lower (p = 0.07), and propionate was higher (p = 0.02) for RS-diet than MH-diet. Concentrations of plasma lactate, non-esterified fatty acids, Leu and ${\alpha}$-ketoisocaproic acid did not differ (p>0.10) between the diets, but plasma glucose and urea concentrations were lower (p = 0.01 and p = 0.003, respectively) for RS-diet than MH-diet. Turnover rate of plasma acetate did not differ (p = 0.39) between the diets, and plasma glucose and Leu turnover rates were numerically lower (p = 0.15 and p = 0.14, respectively) for RS-diet than MH-diet. Whole body protein synthesis and degradation did not differ (p>0.10) between the diets. Thus it can be concluded that the intermediary metabolism of acetate, glucose and protein on rice straw is comparable to mixed hay in sheep.

• Journal of Animal Science and Technology
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• 제59권6호
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• pp.14.1-14.9
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• 2017

Background: Garlic and its constituents are reported to have been effective in reducing methane emission and also influence glucose metabolism in body; however, studies in ruminants using garlic leaves are scarce. Garlic leaves contain similar compounds as garlic bulbs, but are discarded in field after garlic bulb harvest. We speculate that feeding garlic leaves might show similar effect as garlic constituents in sheep and could be potential animal feed supplement. Thus, we examined the effect of freeze dried garlic leaves (FDGL) on rumen fermentation, methane emission, plasma glucose kinetics and nitrogen utilization in sheep. Methods: Six sheep were fed Control diet (mixed hay and concentrate (60:40)) or FDGL diet (Control diet supplemented with FDGL at 2.5 g/kg $BW^{0.75}$ of sheep) using a crossover design. Methane gas emission was measured using open-circuit respiratory chamber. Plasma glucose turnover rate was measured using isotope dilution technique of [$U-^{13}C$]glucose. Rumen fluid, feces and urine were collected to measure rumen fermentation characteristics and nitrogen utilization. Result: No significant difference in rumen fermentation parameters was noticed except for rumen ammonia tended to be higher (0.05 < P < 0.1) in FDGL diet. Methane emission per kg dry matter ingested and methane emission per kg dry matter digested were lower (P < 0.05) in FDGL diet. Plasma glucose concentration was similar between diets and plasma glucose turnover rate tended to be higher in FDGL diet (0.05 < P < 0.1). Nitrogen retention was higher (P < 0.05) and microbial nitrogen supply tended to be higher (0.05 < P < 0.1) in FDGL diet. Conclusion: FDGL diet did not impair rumen fermentation, improved nitrogen retention; while absence of significant results in reduction of methane emission, glucose turnover rate and microbial nitrogen supply, further studies at higher dose would be necessary to conclude the merit of FDGL as supplement in ruminant feedstuff.

• Asian-Australasian Journal of Animal Sciences
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• 제16권3호
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• pp.380-383
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• 2003

The gas dilution technique was used to evaluate the possibility of estimating the volume of gaseous phase in the rumen from its composition in sheep given rice whole crop silage (RWS) or dent corn silage (DCS) at a level of maintenance (M) or 2 M, and in the course of fasting. The rumen gas composition was determined at 2 and 7.5 h after morning feeding. Nitrogen gas was injected by using an airtight syringe into the rumen immediately after collecting the rumen gas sample as a control. Then rumen gas samples were collected at 5, 10, 20, 40 and 60 min. after injection. Dry-matter intakes were $42g/kg^{0.75}$ and $57g/kg^{0.75}$ for DCS, and $36g/kg^{0.75}$ and $59g/kg^{0.75}$ for RWS, at 1 M and 2 M levels, respectively. Animals ingested both silages about 20% less than expected at 2 M level. The rumen gas composition did not differ significantly between 2 h and 7.5 h after feeding except for $N_2$. Content of $CO_2$ in gas composition was significantly higher at 2 M level than at 1 M (p<0.05) for both RWS and DCS, whereas $CH_4$ showed no significant difference between feeding levels. At both feeding levels, $CO_2$ showed a higher (p<0.05) percentage in DCS than RWS. A dilution technique by using $N_2$ injection is not appropriate for the determination of gas production in vivo, unless the rate of rumen gas turnover is considered. Changes in composition at fasting indicate that the rumen fermentation may reach the lowest level after 72 h fasting for sheep given silage as their sole diet.

• Asian-Australasian Journal of Animal Sciences
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• 제20권10호
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• pp.1517-1524
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• 2007

The [$^2H_5$]phenylalanine model was compared with the [1-$^{13}C$]leucine method to determine whole body protein synthesis (WBPS) and degradation (WBPD) in sheep fed at two levels. The animals were fed either 103 (M-diet) or 151 (H-diet) kcal $ME/kg^{0.75}/day$ once daily in a crossover design for 21 days each. The isotope dilutions were simultaneously conducted as a primed-continuous infusion of [$^2H_5$]phenylalanine, [$^2H_2$]tyrosine and [1-$^{13}C$]leucine on each dietary treatment. The WBPS and WBPD calculated from the [$^2H_5$]phenylalanine model were lower (p = 0.009 and p = 0.003, respectively) than those calculated from the [1-$^{13}C$]leucine method. The WBPS tended to be higher (p = 0.08) and WBPD was numerically higher (p = 0.33) for H-diet than M-diet in the [$^2H_5$]phenylalanine model, whereas the WBPS was numerically higher (p = 0.37) for H-diet and WBPS remained similar (p = 0.79) between diets in the [1-$^{13}C$]leucine method. However, the absolute values and the directions of WBPS as well as WBPD from M-diet to H-diet were comparable between the [$^2H_5$]phenylalanine model and [1-$^{13}C$]leucine method. Moreover, the values vary depending on the use of the respective amino acid contents in the carcass protein when calculating WBPS and WBPD. Therefore, it is concluded that the [$^2H_5$]phenylalanine model could be used as an alternative to the [1-$^{13}C$]leucine method for the determination of WBPS and WBPD in sheep.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제12권sup1호
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• pp.53-61
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• 2009

The mainstay in the management of mild to moderately dehydrated children is fast rehydration by using hypotonic ORS (oral rehydration solution) and complete resumption of normal diet, including lactose-containing formula after 4 hours rehydration. Since the majority of young children with uncomplicated acute diarrhea will tolerate large amounts of undiluted non-human milk, withholding food and milk from children during diarrhea is not recommended anymore, regarding time to resolution and diarrhea control. In addition, routine dilution of milk and routine use of lactose-free formula are not necessary after fast ORS therapy. Breastfed infants and children fed with solid foods may safely continue receiving their usual diets during diarrhea instead of gradual reintroduction of feeding. However, young infants or children with severe diarrhea or malnutrition should be carefully treated under supervision if fed with lactose containing, non-human milk exclusively.

• Asian-Australasian Journal of Animal Sciences
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• 제2권4호
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• pp.615-623
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• 1989

Three sheep fitted with rumen cannulae and abomasal cannulae were given daily 750 g (DM) of three diets consisting of straw-manure silage and barley mixture in the ratios of 75:25, 50:50 and 25:75. As the proportion of barley in the diet increased, there was an increase in the amount of OM apparently digested in the rumen and thole tract (P<.01). But ADF digestion was decreased. For the 25:75 diet the $NH_3-N$ content in the rumen showed the highest value, but the total VFA was the lowest. The rumen volume and dilution rate increased with increasing ratio of silage in diets. There were no significant differences between diets in abomasal NAN flow, and the bacterial-N for the 25:75 diet was 7.3 g N as compared with 9.2-9.6 g N for the other diets (P<.01). Rates of bacterial nitrogen synthesis in the rumen were 30.5, 24.1 and 14.9 g N per Kg OM apparently digested in the rumen for the 75:25, 50:50 and 25:75 diets, respectively.