• Title/Summary/Keyword: diesel degradation

Search Result 14, Processing Time 0.153 seconds

Evaluation of Bioremediation Effectiveness by Resolving Rate-Limiting Parameters in Diesel-Contaminated Soil

  • Joo, Choon-Sung;Oh, Young-Sook;Chung, Wook-Jin
    • Journal of Microbiology and Biotechnology
    • /
    • v.11 no.4
    • /
    • pp.607-613
    • /
    • 2001
  • The biodegradation rates of diesel oil by a selected diesel-degrading bacterium, Pseudomonas stutzeri strain Y2G1, and microbial consortia composed of combinations of 5 selected diesel-degrading bacterial were determined in liquid and soil systems. The diesel degradation rate by strain Y2G1 linearly increased $(R^2=0.98)$ as the diesel concentration increased up to 12%, and a degradation rate as high as 5.64 g/l/day was obtained. The diesel degradation by strain Y2G1 was significantly affected by several environmental factors, and the optimal conditions for pH, temperature, and moisture content were at pH8, $25^{\circ}C$, and 10%, respectively. In the batch soil microcosm tests, inoculation, especially in the form of a consortium, and the addition of nutrients both significantly enhanced the diesel degradation by a factor of 1.5 and 4, respectively. Aeration of the soil columns effectively accelerated the diesel degradation, and the initial degradation rate was obviously stimulated with the addition of inorganic nutrients. Based on these results, it was concluded that the major rate-limiting factors in the tested diesel-contaminated soil were the presence of inorganic nutrients, oxygen, and diesel-degrading microorganisms. To resolve these limiting parameters, bioremediation strategies were specifically designed for the tested soil, and the successful mitigation of the limiting parameters resulted in an enhancement of the bioremediation efficiency by a factor of 11.

  • PDF

Optimization of diesel biodegradation by Vibrio alginolyticus using Box-Behnken design

  • Imron, Muhammad Fauzul;Titah, Harmin Sulistiyaning
    • Environmental Engineering Research
    • /
    • v.23 no.4
    • /
    • pp.374-382
    • /
    • 2018
  • Petroleum hydrocarbons pollutants, such as diesel fuel, have caused ecosystem damage in terrestrial and aquatic habitats. They have been recognized as one of the most hazardous wastes. This study was designed to optimize the effect of Tween 80 concentration, nitrogen (N)/phosphorus (P) ratio and salinity level on diesel biodegradation by Vibrio alginolyticus (V. alginolyticus). Response surface methodology with Box-Behnken design was selected with three factors of Tween 80 concentration (0, 5, 10 mg/L), N/P ratio (5, 10, 15) and salinity level (15‰, 17.5‰, 20‰) as independent variables. The percentage of diesel degradation was a dependent variable for 14 d of the remediation period. The results showed that the percentages of diesel degradation generally increased with an increase in the amount of Tween 80 concentration, N/P ratio and salinity level, respectively. The optimization condition for diesel degradation by V. alginolyticus occurred at 9.33 mg/L of Tween 80, 9.04 of N/P ratio and 19.47‰ of salinity level, respectively, with percentages of diesel degradation at 98.20%. The statistical analyses of the experimental results and model predictions ($R^2=0.9936$) showed the reliability of the regression model and indicated that the addition of biostimulant can enhance the percentage of diesel biodegradation.

Biodegradation of Diesel Oil by Microorganisms Isolated from Petroleum Contaminated Site (유류 오염지역으로부터 분리된 균주를 이용만 디젤유의 분해)

  • 박천보;허병기;윤현식
    • KSBB Journal
    • /
    • v.16 no.6
    • /
    • pp.632-637
    • /
    • 2001
  • The cells obtained from diesel contaminated site were tested for diesel degradation by culturing them on the culture medium that contained diesel as the only carbon source. Two strains that grew well in the culture media were separated: one formed white colony and another strain formed yellow colony. When they were cultured together, much higher diesel degradation was obtained compares to that of individual cell culture. Mixed culture of white and yellow colony forming strains grew well with 1%(v/v) diesel and the addition of growth nutrients increased the diesel degradation. Additional nitrogen source was efficient for higher diesel degradation (over 90%) when it was compared with that without nitrogen source. When mixed culture of white and yellow colony forming cells were applied to the soil column system contaminated by diesel, 30 mL/min of air flow rate was found to be sufficient to degrade diesel oil. The diesel degradation did not increase noticeably at higher flow rate. The addition of nitrogen source resulted in the increase in diesel degradability.

  • PDF

Bio Sparging Column Experiment for Remediation of Diesel Contaminated Groundwater (디젤오염 지하수 정화를 위한 공기주입정화법 칼럼 실험)

  • Chang Soon-Woong;Lee Si-Jin;Song Jung-Hoon;Kwon Soo-Youl
    • Journal of Environmental Science International
    • /
    • v.13 no.12
    • /
    • pp.1059-1065
    • /
    • 2004
  • Bio sparging experiments were conducted in a laboratory column to investigate the potential removal of diesel contaminated groundwater. The objectives in this study were (a) to determine the extent of diesel degradation in laboratory columns under supplement of nutrient; (b) to determine the effect of variation of air flow in the removal of diesel and (c) to evaluate the potential enhancement of diesel degradation as a function of temperature. Our results showed that the nutrient supplement and higher air flow greatly enhanced diesel degradation. However, the variation of water temperature examined slightly increased degradation rate of diesel fuel.

A Study on Microbial Community and Microbial Degradation of Diesel (디젤의 미생물 분해와 군집에 관한 연구)

  • Choi, Hee-Chol;Cho, Yoon-A;Choi, Sang-Il;Lee, Tae-Jin
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.32 no.5
    • /
    • pp.509-516
    • /
    • 2010
  • This study investigates characteristics of diesel degradation and variations of microbial community with the soil enrichment cultures. The cultures has yellow(YE-5) and transparent color's(WH-5) colony on solid plate medium. The bacillus type of YE-5 and WH-5 cultures showed diesel degradation at the rate of 99.07mg-Diesel/$L{\cdot}day$ and 57.82mg-Diesel/$L{\cdot}day$ in the presence of 1%(v/v) initial diesel concentration. Diesel degradation was 1.7 times faster than WH-5 culture. YE-5 or WH-5 culture could degrade a wide range of diesel compounds from $C_8$ to $C_24$. Microbial community analysis by PCR-DGGE technique shows that Psedomonas, Klebsiella, Escherichia and Stenotrophomonas as proteobacteria take role on the diesel degradation. uncultured Senotrophomonas sp. was only detected with YE-5 culture. It is concluded that proper combination of the microorganism should be present to stimulate the degradation of diesel and further studies are recommended for the effect of uncultured Senotrophomonas sp. or Escherichia hermannii on diesel degradation.

Biodegradation Kinetics of Diesel in a Wind-driven Bioventing System

  • Liu, Min-Hsin;Tsai, Cyuan-Fu;Chen, Bo-Yan
    • Journal of Soil and Groundwater Environment
    • /
    • v.21 no.5
    • /
    • pp.8-15
    • /
    • 2016
  • Bioremediation, which uses microbes to degrade most organic pollutants in soil and groundwater, can be used in solving environmental issues in various polluted sites. In this research, a wind-driven bioventing system is built to degrade about 20,000 mg/kg of high concentration diesel pollutants in soil-pollution mode. The wind-driven bioventing test was proceeded by the bioaugmentation method, and the indigenous microbes used were Bacillus cereus, Achromobacter xylosoxidans, and Pseudomonas putida. The phenomenon of two-stage diesel degradation of different rates was noted in the test. In order to interpret the results of the mode test, three microbes were used to degrade diesel pollutants of same high concentration in separated aerated batch-mixing vessels. The data derived thereof was input into the Haldane equation and calculated by non-linear regression analysis and trial-and-error methods to establish the kinetic parameters of these three microbes in bioventing diesel degradation. The results show that in the derivation of μm (maximum specific growth rate) in biodegradation kinetics parameters, Ks (half-saturation constant) for diesel substance affinity, and Ki (inhibition coefficient) for the adaptability of high concentration diesel degradation. The Ks is the lowest in the trend of the first stage degradation of Bacillus cereus in a high diesel concentration, whereas Ki is the highest, denoting that Bacillus cereus has the best adaptability in a high diesel concentration and is the most efficient in diesel substance affinity. All three microbes have a degradation rate of over 50% with regards to Pristane and Phytane, which are branched alkanes and the most important biological markers.

Biodegradation of Diesel by Rhodococcus fascians in Sand Column (Rhodococcus fascians를 이용한 모래 컬럼내 디젤유 분해)

  • Moon, Jun-Hyung;Koo, Ja-Ryong;Yun, Hyun-Shik
    • KSBB Journal
    • /
    • v.26 no.1
    • /
    • pp.1-6
    • /
    • 2011
  • Contamination of soils, groundwater, air and marine environment with hazardous and toxic chemicals is major side effect by the industrialization. Bioremediation, the application of microorganism or microbial processes to degrade environmental contaminant, is one of the new environmental technologies. Because of low water solubility and volatility of diesel, bioremediation is more efficient than physical and chemical methods. The purpose of this study is biodegradation of diesel in sand by using Rhodococcus fascians, a microorganism isolated from petroleum contaminated soil. This study was performed in the column containing sand obtained from sea sides. Changes in biodegradability of diesel with various flow rates, inoculum sizes, diesel concentrations, and pH were investigated in sand column. The optimal condition for biodegradation of diesel by R. fascians in sand column system was initial pH 8 and air flow rate of 30 mL/min. Higher diesel degradation was achieved at larger inoculum size and the diesel degradation by R. fascians was not inhibited by diesel concentration up to 5%.

Effects of the Presence of Nonionic surfactants on Diesel Biodegradation (비이온계 계면활성제가 미생물의 디이젤 분해에 미치는 영향)

  • Lee, Hyo-Sang;Jeong, Gi-Hyeong;Kim, Jeong-Rak;Lee, Gi-Se
    • 한국생물공학회:학술대회논문집
    • /
    • /
    • pp.424-425
    • /
    • 2000
  • The effects of the presence of commercial non-ionic surfactants on the cell growth and diesel degradation by Pseudomonas sp. OSD were studied. Most surfactants inhibited the diesel biodegradation at high concentration(1000mg/1). However, some surfactants showed no inhibition at lower concentrations. Tween 20, Brij 58, Brij 78 were not inhibitory to the diesel biodegradation even at high concentration. These chosen surfactants has relatively high HLB values. There exists complicated relationship for diesel bioremediation between cell hydrophobicity, surfactant HLB, contaminants, an soil.

  • PDF

Investigating Biochemical Properties of Bacillus aryabhattai DA2 from Diesel-Contaminated Soil

  • Kim, Sang-Jun;Adhikari, Arjun;Lee, Ko-Eun;Joo, Gil-Jae
    • Korean Journal of Environmental Biology
    • /
    • v.36 no.2
    • /
    • pp.199-205
    • /
    • 2018
  • Petroleum energy is the major source of the world energy market, and its massive usage, and the corresponding extreme environmental pollution, imposes a serious threat on the ecological cycles. By screening oil-contaminated soil, we isolated, identified, and characterized a novel strain that represents a considerable diesel-degrading potentiality; the Bacillus aryabhattai DA2 strain is registered in the NCBI with the accession number MG571630, and it possesses an efficient tributyrin-degrading capacity. The optimal condition for diesel degradation by DA2 strain was observed at pH between 7-8 and at the temperature of $30^{\circ}C$. The strain is resistant to salt as well as the antibiotics like ampicillin and streptomycin. These results indicate B. aryabhattai is one of the potential candidates for the remediation of the diesel-contaminated sites.

Remediation of Diesel-Contaminated Soil by Electrokinetically Supplied Bacterial Cells

  • 이효상;이기세
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
    • /
    • /
    • pp.20-23
    • /
    • 2000
  • The use of electrokinetic injection and transport for the distribution of an NAPLs-degrading microorganism in a sandy soil bed was studied. After the injection of the cell into cathode side of bed, an electric current was applied. The transport of cell though the sandy soil was achieved by electokinetics, mainly by electrophoresis, The pH control in electrode chamber plays un important role to achieve desirable cell transport because H$^{+}$ generated at anode is toxic or inhibits the transport of cells. Electokinetic distribution rate of bacterial cells changed depending on the applied electric current and pH. The degradation of diesel by electrokinetically transport cells were monitored.d.

  • PDF