• Toxicological Research
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• v.9 no.2
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• pp.253-262
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• 1993

The effects of dichloromethane (DCM) on carbon tetrachloride (CT) toxicity were examined in adult male rats. A concomitant treatment of rats with DCM (0.3, 0.6, 1.2 g/kg, po) significantly potentiated the hepatotoxicity of CT (1.0 g/kg, po) as determined by increase in serum GPT (glutamic pyruvic transaminase), GOT (glutamic oxaloacetic transaminase), and SDH (sorbitol dehydrogenase) activity 24 hr following the treatments. Serum LDH (lactate dehydrogenase) activity was increased by either DCM or CT treatment.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.151.2-151.2
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• 2003

For the purpose of the development of a skin-whitening agent, Sophora flavescens was evaluated for tyrosinase inhibitory activity and its active principles were identified followed activity-guided isolation. The ethanol extract and dichloromethane fraction from S. flavescens showed significant inhibition of mushroom tyrosinase. From the dichloromethane fraction, three known prenylated flavonoids, sophoraflavanone G, kuraridin, and kurarinone, were isolated. Compared with kojic acid ($IC_50$=20.5 $\mu$M), these compounds possessed more potent tyrosinase inhibitory activity. (omitted)

• Korean Chemical Engineering Research
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• v.56 no.4
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• pp.461-468
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• 2018

Microspheres were prepared by solvent-evaporation method with Poly-L-lactic acid (PLLA) as a starting material, and the effects of preparation variables on microsphere shape and average particle size were investigated. As the concentration of PVA solution increased from 1 to 5 wt%, the average particle size decreased from $370{\mu}m$ to $160{\mu}m$ and then increased to $240{\mu}m$ at 7 wt%. On the other hand, As the addition volume of PVA solution increased from 10 mL to 50 mL, the average particle size decreased from $370{\mu}m$ to $220{\mu}m$. Also, as the stirring speed increased from 500 rpm to 1,500 rpm, the average particle size decreased from $370{\mu}m$ to $110{\mu}m$. When dichloromethane and chloroform were used as organic solvents, respectively, the average particle size did not show any significant difference. However, when dichloromethane was used, voids were observed on the particle surface, but when chloroform was used, smooth spherical particles were obtained.

• Membrane Journal
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• v.17 no.3
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• pp.184-190
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• 2007

This study reports on the pervaporation separation of a volatile organic compound(VOC), dichloromethane(DCM) from water using fluorinated copolysiloxaneimide membranes. The copolysiloxaneimide membranes were prepared from 4,4'-(hexafluoroisopropylidene)diphthalic anhydride(6FDA) and two diamines(polysiloxane diamine(SIDA), 2-(perfluorohexyl)ethyl-3,5-diaminobenzene(PFDAB)). By varying the ratio of flexible polysiloxane diamine(SIDA)/rigid fluorinated aromatic diamine(PFDAB) from 0/100 to 100/0 mol%, five copolysiloxaneimide membranes were prepared success- fully. The pervaporation properties of DCM/water were examined in terms of two diamine monomer ratio at room temperature and the feed composition of 0.05 wt% in water. It was found that the increase in SIDA content led to high permeation flux and pervaporation selectivity towards DCM by the enhanced sorption/sorption selectivity and diffusion coefficient/diffusion selectivity due to the increased hydrophobicity and fractional free volume.

• Journal of the Korean Applied Science and Technology
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• v.16 no.2
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• pp.179-185
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• 1999

The effect of blowing agents and inner temperature on the machanical properties of the flexible polyurethane foams were investigated. In the study used that chemical blowing agents is $H_2O$ and support blowing agents. CFC-11, HCFC-114b, dichloromethane, n-penthane, iso-pentane, cyclopentane. The flexible polyurethane foams were foamed by the density of $0.015{\pm}0.002g/cm^3$ and $0.024{\pm}0.002g/cm^3$ which were used in mechanical properties measurements. Inner temperature was measure as long as the preparation of the flexible polyurethane foams of each blowing agents. The density, tensile strength, elongation, tear strength, compression strength and compression set were measured after 48 hours hardening. The result of the study was optimized dichloromethane and cyclopentane at the support blowing agents.

• Korean Journal of Microbiology
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• v.53 no.3
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• pp.193-199
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• 2017

This study was aimed to evaluate the antimicrobial activity of Penicillium purpurogenum ED76 on several clinically important microorganisms. The endophytic fungus P. purpurogenum ED76 was previously isolated from Swietenia macrophylla leaf. The antimicrobial efficacy of P. purpurogenum ED76 dichloromethane extract was determined via disc diffusion and broth microdilution assay. A kill curve study was conducted and the morphology of extract treated bacterial cells were viewed under scanning electron microscope. The dichloromethane extract showed significant inhibitory activity on 4 test bacteria and 2 test yeasts. The minimal inhibitory concentration of the extract ranged from 125 to $1,000{\mu}g/ml$, which indicates the different susceptibility levels of the test microorganisms to the fungal extract. The kill curve study has revealed a concentration-dependent inhibition for all test microorganisms. With the increase of the extract concentration, the microbial growth was significantly reduced. The scanning electron micrograph of dichloromethane extract-treated Staphylococcus aureus cells showed the total damage of the cells. The cell wall invagination of the bacterial cells also indicates the loss of cellular materials and metabolic activity. The gas chromatography mass spectrometry analysis of the extract also showed that the major compound was stigmasterol, which constitutes 45.30% of the total area. The dichloromethane extract of P. purpurogenum ED76 exhibited significant inhibitory activity on several clinically important bacteria and yeasts. The study proposed a possible mode of action that the extract cause significant damage to the morphology of S. aureus cells.

• KSBB Journal
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• v.24 no.1
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• pp.47-52
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• 2009

A pilot-plant biofilter (1750 mm W ${\times}$ 2750 mm L ${\times}$ 2000 mm H) packed with polyurethane foam (20 mm W ${\times}$ 20 mm L ${\times}$ 20 mm H) was installed in an fine chemical plant emitting gas streams containing ethyl alcohol, ethyl acetate, and dichloromethane. The biofilter was successfully operated for 30 days under highly fluctuating incoming concentrations (maximum 3500 ppm) at a residence time of 36 and 60 sec. Both ethyl alcohol and ethyl acetate were removed more than 95%, but dichloromethane removal was less than 50%. Malodor was also removed more than 90% from 17 days after start up.

• Toxicological Research
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• v.27 no.2
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• pp.77-83
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• 2011

The objective of this study was to evaluate and compare the antitumor activity of different solvent fractions (ethanol, dichloromethane, ethyl acetate, butanol and water) of the Auricularia auricula-judae 70% ethanol extract on the P388D1 macrophage and sarcoma 180 cells. A dose-dependent antitumor activity of each solvent fraction (from 0.01 mg/ml to 0.3 mg/ml) was shown against both cell types. These cytotoxic effects of all the tested fractions were confirmed on the MTT and SRB assays, without statistical differences each other. $IC_{50}$ value of dichloromethane fraction was 94.2 ${\mu}g/ml$ against sarcoma 180 cells lower than any other solvent fractions. The potent antitumor effect of the dichloromethane (DCM) fraction was also found against solid tumor in BALB/c mice. The splenomegaly and higher splenic index were found in tumor-bearing mice, with the DCM fraction returning to the negative control values. Thus, the results indicated the dichloromethane fraction may have potential ingredients as antitumor candidates.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.5
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• pp.900-907
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• 2000

The antioxidant effects of dichloromethane, ethylacetate or water fraction of kimchi added to the 1% cholesterol diet were studied. Six New Zealand white rabbits in each group were fed either control diet (basal diet containing 1% cholesterol) or experimental diet containing dichloromethane (CH$_2$Cl$_2$), ethylacetate (EtOAc) or water ($H_2O$) fraction of kimchi in the control diet for 16 weeks. The amount of each solvent fraction of kimchi added to the experimental diet was equivalent to 5% of freeze-dried kimchi. Levels of hepatic lipid oxidation expressed as TBARS or peroxide value for the experimental groups were lower than that of control (p<0.05). Liver homogenated of the experimental group containing dichloromethane fraction of kimchi inhibited LDL oxidation in the presence of Cu++ by 46% (p<0.05). The activities of catalase, Glutathione peroxidase (GSH-Px), Cu, Zn-superoxide (Cu, Zn-SOD) and Mn-superoxide (Mn-SOD) of experimental groups were lower than those of control group. Low enzyme activities observed from the kimchi solvent fraction groups might be due to the level of lipid oxidation progressed less in these groups. The most significant antioxidant effects were observed from dichloromethane fraction of kimchi among the experimental groups. The major fatty acids of hepatic phospholipid of rabbit were C18:2 and C18:0. But the major fatty acid profile was changed into C16:0, C18:0, C18:1, and C18:2 when rabbit was fed 1% cholesterol diet for 16 weeks, and this profile was almost the same as in rabbit fed diet containing kimchi solvent fraction. The ratio for unsaturated fatty acid to saturated fatty acid decreased by cholesterol induced diet and it was not corrected by kimchi solvent fractions.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.673-675
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• 2003

The antioxidant activities of methanol and dichloromethane extracts of eighteen salt marsh plants were tested by using 1,1-Diphenyl-2-picryl-hydrazyl(DPPH) at a $100{\mu}g/ml$ concentrations. The methanol extracts of four salt marsh plants(Rosa rugosa Thunberg, Erigeron annuus, Ixeris tamagawaensis kitamura, Artemisia capillaris Thunberg) were found to be most effective in DPPH radical scavenging activity. The DPPH radical scavenging effect of these plants was Artemisia capillaris Thunberg(88.67%), Rosa rugosa Thunberg(87.51%), Erigeron annuus(78.49%), Ixeris tamagawaensis kitamura(69.99%) in order and was comparable with that of natural antioxidant, L-ascorbic acid(96.64%). The next effective ones were the methanol extracts of Teragonia tetragonoides(58.66%) and the dichloromethane extracts of Artemisia capillaris Thunberg(54.67%) and the others did not show a considerable activity. These salt marsh plants were evaluated using the pyrogallol UV-VIS spectrophotometeric method to generate superoxide anion. Among them, the dichloromethane extracts of four salt marsh plants and the methanol extracts of fifteen ones were weak superoxide dismutase-like activities.