• Title/Summary/Keyword: diatom analysis

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Colonization and Succession of Epilithic Diatoms on Artificial Substrata (인공기질에서의 부착규조의 초기 군집형성과 천이)

  • Jang, Sung-Hyun;Lee, Jung-Hoon;Lee, Jung-Ho
    • ALGAE
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    • v.21 no.3
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    • pp.295-305
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    • 2006
  • The purpose of the study is to understand the ecological characteristics of the early stage of community formation of epilithic diatoms. The samples were daily collected from a site of the Kumho River during 70 days from October 13 to December 21 in 2002. Total 126 diatom taxa collected in the study were classified into 114 species and 12 varieties belonging to 26 genera. About 80% of the taxa collected from the artificial substrata during the whole studying period were observed until tenth day from the start time of the study. In the study the successional process of the diatom community was divided into the three stages of the early, the middle and the later by cluster analysis based on the similarities in species composition among the communities. Cocconeis placentula Ehrenb. and C. placentula var. lineata (Ehrenb.) V.H., horizontally positioned species, would be divided into the early successional species, and Diatoma vulgre Bory, vertically positioned species, was the later successional species. Cell densities and species composition of the diatoms on the artificial substrata were closely similar to them of natural substrata after 40th day from the start time of the study.

Molecular profiling of 18S rRNA reveals seasonal variation and diversity of diatoms community in the Han River, South Korea

  • Muhammad, Buhari Lawan;Lee, Yeon-Su;Ki, Jang-Seu
    • Journal of Species Research
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    • v.10 no.1
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    • pp.46-56
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    • 2021
  • Diatoms have been used in examining water quality and environmental change in freshwater systems. Here, we analyzed molecular profiling of seasonal diatoms in the Han River, Korea, using the hypervariable region of 18S V1-V3 rRNA and pyrosequencing. Physicochemical data, such as temperature, DO, pH, and nutrients showed the typical seasonal pattern in a temperate region. In addition, cell counts and chlorophyll-a, were recorded at high levels in spring compared to other seasons, due to the diatom bloom. Metagenomic analysis showed a seasonal variation in the phytoplankton community composition, with diatoms as the most frequently detected in spring (83.8%) and winter (69.7%). Overall, diatom genera such as Stephanodiscus, Navicula, Cyclotella, and Discostella were the most frequent in the samples. However, a large number of unknown Thalassiosirales diatoms were found in spring (35.5%) and winter (36.3%). Our molecular profiling revealed a high number of diatom taxa compared to morphological observation. This is the first study of diatoms in the Han River using molecular approaches, providing a valuable reference for future study on diatoms-basis environmental molecular monitoring and ecology.

Annotation and Expression Profile Analysis of cDNAs from the Antarctic Diatom Chaetoceros neogracile

  • Jung, Gyeong-Seo;Lee, Choul-Gyun;Kang, Sung-Ho;Jin, Eon-Seon
    • Journal of Microbiology and Biotechnology
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    • v.17 no.8
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    • pp.1330-1337
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    • 2007
  • To better understand the gene expression of the cold-adapted polar diatom, we conducted a survey of the Chaetoceros neogracile transcriptome by cDNA sequencing and expression of interested cDNAs from the Antarctic diatom. A non-normalized cDNA library was constructed from the C. neogracile, and a total of 2,500 cDNAs were sequenced to generate 1,881 high-quality expressed sequence tags (ESTs) (accession numbers EL620615-EL622495). Based on their clustering, we identified 154 unique clusters comprising 342 ESTs. The remaining 1,540 ESTs did not cluster. The number of unique genes identified in the data set is thus estimated to be 1,694. Taking advantage of various tools and databases, putative functions were assigned to 939 (55.4%) of these genes. Of the remaining 540 (31.9%) unknown sequences, 215 (12.7%) appeared to be C. neogracile-specific since they lacked any significant sequence similarity to any sequence available in the public databases. C. neogracile consisted of a relatively high percentage of genes involved in metabolism, genetic information processing, cellular processes, defense or stress resistance, photosynthesis, structure, and signal transduction. From the ESTs, the expression of these putative C. neogracile genes was investigated: fucoxanthin chlorophyll (chl) a,c-binding protein (FCP), ascorbate peroxidase (ASP), and heat-shock protein 90 (HSP90). The abundance of ASP and HSP90 changed substantially in response to different culture conditions, indicating the possible regulation of these genes in C. neogracile.

Effect of Pretreatment of Biogenic Titanium Dioxide on Photocatalytic Transformation of Chloroform (Biogenic TiO2 나노입자 전처리가 클로로포름 광분해에 미치는 영향)

  • Kwon, Sooyoul;Rorrer, Greg;Semprini, Lewis;Kim, Young
    • Journal of Korean Society on Water Environment
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    • v.27 no.1
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    • pp.98-103
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    • 2011
  • Photocatalysis using UV light and catalysts is an attractive low temperature and non-energy- intensive method for remediation of a wide range of chemical contaminants like chloroform (CF). Recently development of environmental friendly and sustainable catalytic systems is needed before such catalysts can be routinely applied to large-scale remediation or drinking water treatment. Titanium dioxide is a candidate material, since it is stable, highly reactive, and inexpensive. Diatoms are photosynthetic, single-celled algae that make a microscale silica shell with nano scale features. These diatoms have an ability to biologically fabricate $TiO_2$ nanoparticles into this shell in a process that parallels nanoscale silica mineralization. We cultivated diatoms, metabolically deposited titanium into the shell by using a two-stage photobioreactor and used this biogenic $TiO_2$ to this study. In this study we evaluated how effectively biogenic $TiO_2$ nanoparticles transform CF compared with chemically-synthesized $TiO_2$ nanoparticlesthe and effect of pretreatment of diatom-produced $TiO_2$ nanoparticles on photocatalytic transformation of CF. The rate of CF transformation by diatom-$TiO_2$ particles is a factor of 3 slower than chemically-synthesized one and chloride ion production was also co-related with CF transformation, and 79~91% of CF mineralization was observed in two $TiO_2$ particles. And the period of sonication and mass transfer due to particle size, evaluated by difference of oxygen tention does not affect on the CF transformation. Based on the XRD analysis we conclude that slower CF transformation by diatom-$TiO_2$ might be due to incomplete annealing to the anatase form.

The Change of the Depositional Environment on Dodaecheon River Basin during the Middle Holocene (Holocene 中期에 있어서 道垈川流域의 堆積 環境 變化)

  • Hwang, Sang-Ill;Yoon, Soon-Ock;Jo, Wha-Ryong
    • Journal of the Korean Geographical Society
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    • v.32 no.4
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    • pp.403-420
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    • 1997
  • Dodaecheon is a small river flowing into Asan Bay which is located in the middle part of the West Coast of the Korean Penninsula. We have investigated the change of depositional environment in Dodaecheon river basin during the middle Holocene. In the course of the research, the methods such as boring, radiocarbon dating, diatom and pollen analysis were employed. The Holocene deposits of the studied area are consisted of peat and gray silt layers, and contain many plooen and diatom fossils. Based on the results of diatom and pollen analysis, we conclude that the gray silt layers were sedimented owing to the transgression in the middle Holocene, and the peat layers by the regression or stabilzation of the sea level. The shoreline in the Post Glacial Age reached to the rivemouth of Dodaecheon at ca. 7,000 years before present(y. BP) and at that time the high tide sea-level(mean high water level of spring tide) rose to ca. 3m above present mean sea-level(m.a.s.l.). Since then to ca. 6,000y. BP, the high tide sea-level arrived to ca. 5m above present mean sea level further repeating minor transgressions and regressions. The peat layers of the coastal lowland of the West Coast were formed by the sea level fluctuations from 7,000 y. BP to 3,000 y. BP, and they were distributed 2 to 6 meters higher than the mean sea level of the present day. Most of them sedimented due to the high tide level are older and higher than those of the East Coast which were formed at the swale in the low tidal range environment.

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Use of Benthic Algae and Bryophytes for Monitoring Rivers

  • Whitton, Brian A.
    • Journal of Ecology and Environment
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    • v.36 no.1
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    • pp.95-100
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    • 2013
  • Many countries have adopted a single, well-described approach to the use of phototrophs for monitoring river water quality, which involves the use of indices related to diatom composition at a site. Increasingly these indices have focussed on assessing ambient phosphate concentration. However, there is a wide range of other methods which can provide additional information to make up for any weaknesses in the standard method. Some of these methods are reviewed briefly here. They can be useful, for instance, when considering temporal and spatial variability in phosphate concentration at a particular site and providing much more insight on heavy metal or pesticide pollution than revealed by routine water analysis.

Analysis of Oppositive Interaction between Cd and Zn Toxicity in Coastal Algae (연안 조류에서 Cd와 Zn 독성의 반대 작용 분석)

  • 이봉헌;김정호;정성옥;김성미;박흥재
    • Journal of Environmental Science International
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    • v.11 no.10
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    • pp.1069-1074
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    • 2002
  • The growth and heavy metal experiments revealed oppositive interactions between toxic metals(Zn and Cd) and Mn when the coastal diatom T. pseudonana were used. Cd and Zn inhibited the algal growth rate only at low Mn ion concentrations and this effect could be accounted for an inhibition of cellular Mn take by the toxic metals. Mn and Zn inhibited cellular Cd take and this indicated a reciprocal effects among the metals with respect to metal take. Saturation kinetics modeling of the take data was consistent with two metals competing with each other for binding to the Mn take system and with both Cd and Mn being transported into the cell by that system. Mathematical modeling of Mn and Cd take data revealed evidence fur a Cd efflux system.

Spatial Distribution of Epilithic Diatom Communities in the Estuary of Korean Peninsula (한반도 하구역 부착돌말류의 공간적 분포)

  • Kim, Ha-Kyung;Cho, In-Hwan;Kim, Young-Hyo;Lee, Min-Hyuk;Kim, Yong-Jae;Won, Du-Hee;Hwang, Su-Ok;Byun, Jung-Hwan;Hwang, Soon-Jin;Kim, Baik-Ho
    • Korean Journal of Ecology and Environment
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    • v.51 no.1
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    • pp.1-15
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    • 2018
  • With land-use (cover) and water quality, the distributional characteristics of epilithic diatom communities were studied with 193 samples from estuaries of Korean peninsula between 2015 and 2016. Of total 394 taxa classified, Nitzschia perminuta (19.6%) and N. inconspicua (14.0%) were the 1st and 2nd dominant species. Using a cluster analysis, the epilithic diatom communities of Korean estuaries were divided into four groups (G1-G4). Ecological characteristics of each group were followed: G1 was located in estuaries of the East Sea, and characterized by high forest land-use and high DO and low nutrients; G2 was the eastern part of the South Sea, and characterized by low turbidity and nutrients; G3 was the western part of the South Sea, and characterized by high agriculture, low electric conductivity and low salinity; G4 was the Yellow Sea, and characterized by high nutrients. The environmental factors having significant correlation with diatom distributions were as follows: TN to G1, turbidity to G2, agriculture to G3, and TP to G4. Moreover, the important factors affecting the occurrence of indicator species were forest land-use for Fragilaria construens var. venter in G1, turbidity for Rhoicosphenia abbreviata in G2, urban land- use and total phosphorus (TP) for Bacillaria paradoxa and Hantzschia amphioxys of G3, and TP and turbidity for N. ovalis and Stephanodiscus invistatus of G4. These results collectively indicate that the distribution of epilithic diatom communities in Korean peninsula was largely effected by water quality and land cover/use.

Sea level change and paleogeomorphological change since the middle Holocene in the lowerreaches of Taehwa River, Ulsan-si, South Korea (울산시 태화강 하류부의 Holocene 중기 이래 해수면변동과 고지형변화)

  • Kim, Jeong-Yun;Hwang, Sangill;Yoon, Soon-Ock
    • Journal of The Geomorphological Association of Korea
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    • v.23 no.1
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    • pp.61-75
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    • 2016
  • This study aims to reconstruct sea level change and paleogeomorphology since the middle Holocene by diatom analysis and 14C-dating from the sedimentary facies of three trenches on alluvial plain of Taehwa River, Taehwa-dong, Ulsan-si, South Korea. The Taewha-dong area was a narrow bay located between Bangeojin and eatuary of Taehwa River was a narrow bay which has been detached from the open sea during middle Holocene. Taewa-dong area at bay area was developed into alluvial plain by the sediments had been come from the Taewa river basin. The sea level change during the middle Holocene effected on the development of Taewha-dong alluvial plain and its information is included in the sediment facies.