• Biomolecules & Therapeutics
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• 제26권5호
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• pp.512-519
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• 2018

Phthalates widely used in the manufacture of plastics have deeply penetrated into our everyday lives. Recently, a concern over the toxicity of phthalates on thyroid, has been raised but in most of cases, the doses employed were unrealistically high. To investigate the effects of phthalates on thyroid, we investigated the effects of the repeated oral exposure to low to high doses (0.3, 3, 30 and 150 mg/kg) di-2-ethylhexylphthalate (DEHP) from weaning to maturity for 90 days in juvenile rats on the thyroid. The histological examination revealed that DEHP significantly induced hyperplasia in the thyroid from the doses of 30 mg/kg, which was confirmed with Ki67 staining. In line with this finding, increased mRNA expression of thyrotropin releasing hormone (Trh) was observed in the thyroid of female at 0.3 mg/kg and 150 mg/kg as determined by RNAseq analysis. Moreover, significantly increased expression of parathyroid hormone (Pth) in the female at 0.3 mg/kg, and thyroglobulin (Tg) and thyroid hormone responsive (Thrsp) in the male at 0.3 mg/kg were noted in the blood, of which changes were substantially attenuated at 150 m/kg, alluding the meaningful effects of low dose DEHP on the thyroid hormone regulation. Urinary excretion of mono-2-ethylhexyl-phthalate (MEHP), a major metabolite of DEHP was determined to be 4.10 and 12.26 ppb in male, 6.65 and 324 ppb in female at 0.3 and 30 mg/kg DEHP, respectively, which fell within reported human urine levels. Collectively, these results suggest a potential adverse effects of low dose phthalates on the thyroid.

• 한국지반환경공학회 논문집
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• 제9권7호
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• pp.5-11
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• 2008

본 연구는 청주시 인근의 미호천에서의 프탈레이트의 정성적 특성에 관한 것이다. 프탈레이트 중 DEHP와 DnBP가 가장 많이 사용되어 지고 있다. 미호천 상류와 하류에서 하천수에 함유된 DEHP의 농도는 각각 $12-18{\mu}g/{\ell}$, $11-21{\mu}g/{\ell}$이었다. 또한 이 지역의 퇴적물에서도 DEHP의 농도는 $0.07-0.82{\mu}g/g$(dry), $0.06-0.92{\mu}g/g$(dry), DnBP의 농도는 $0.04-0.25{\mu}g/g$(dry), $0.08-0.34{\mu}g/g$(dry)로 각각 조사 되었다. 공단지역으로부터 유입되는 작은 하천의 하천수에 함유된 DEHP와 DnBP의 농도는 각각 $13-28{\mu}g/{\ell}$, $2-8{\mu}g/{\ell}$이었으며, 퇴적물에서의 농도는 각각 $0.12-0.7{\mu}g/g$(dry), $0.17-2.16{\mu}g/g$(dry)이었다. 미호천과 미호천에 유입되고 있는 하수처리 방류수와 공단천의 프탈레이트 농도는 세계의 다른 지역보다 낮은 수치를 보이고 있다.