• 한국물환경학회지
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• 제24권6호
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• pp.801-805
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• 2008

본 연구에서는 섬유상 재료(PBO)가 고상 추출을 위한 흡착제로 사용되어 분석 과정을 간소화하였다. 두 종류의 PBO섬유(HM, AS)를 이용한 회분식 흡/탈착 실험으로 DEHP 물질의 추출 특성을 평가하였다. 100 mg의 HM 섬유를 20 mL의 수용성 DEHP 용액($50{\mu}g/L$이하)에 주입한 결과, 95%의 DEHP가 흡착되었다. 초기 $50{\mu}g/L$의 DEHP 수용액의 경우, 흡착된 DEHP는 메탄올로 탈착되어 전체 회수율은 92.3%로 나타났다. 따라서 PBO섬유는 알킬프탈레이트 분석을 위한 흡착제로 유용한 것으로 나타났고, 이런 추출 과정은 수중의 SS에 영향을 받지 않았다.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2004년도 춘계학술대회
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• pp.80-80
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• 2004

• 환경위생공학
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• 제17권4호
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• pp.61-67
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• 2002

NIRS(Near infrared spectroscopy) scanning from 1300nm to 2400nm was appl ied for the DEHP(di-(2 ethylhexyl)phthalate) in PVC(polyvinyl chloride_packing material. All samples were devided into calibration group and validation group. As a result of conduction the multiple regression analysis on the correlation between the NIR spectrum data and chemical assay value obtained by the Korea Food Sanitation Act. The validation model for measuring the DEHP content had R of 0.997, SEC of 0.132, SEP of 0.176 by MLR and R of 0.996, SEC of 0.142, SEP of 0.198 by PLS and the detection limit was 0.1%. The obtained results indicate that the NIR procedure can potentially be used as a nondestructive analysis method for the purpose of rapid and simple measurement of DEHP in PVC packing material.

• 한국환경보건학회지
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• 제46권5호
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• pp.548-554
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• 2020

Objectives: This study is to examine the polyvinyl chloride (PVC) materials in elementary school classrooms and libraries in Seoul, and to investigate phthalate contamination in indoor dust. Methods: PVC material was identified for building materials and furniture using portable x-ray fluorescence (XRF). Phthalates in dust samples (n=19) were extracted by ultrasonic extraction using cyclohexane and analyzed by GC-MS. Results: Diethyl phthalate (DEP), di-n-butyl phthalate (DBP), and Bis (2-ethylhexyl) phthalate (DEHP) were found in all collected dust samples (n=19), and diisonyl phthalate (DINP) was detected in all except for one sample (n=18). The concentration of DEHP (median: 2190 mg/kg) and DINP (2960 mg/kg) were higher than other compounds, suggesting that there are many products in the school that used these compounds. When comparing the phthalate concentration in the classroom (n=11) and library dust (n=8), the total concentration in the classroom (median: 10000 mg/kg) was higher than that in the library (8030 mg/kg). DEHP was the dominant compound in the library. The library is relatively more equipped with PVC furniture (n=83) and most floors are also identified as PVC material, suggesting that floors and furniture made of PVC materials are main sources of DEHP contamination. Conclusions: This study is a pilot survey for investigating phthalate contamination in elementary schools. As a result of the survey, phthalate contamination in elementary school was confirmed. However, further study requires risk assessment of children through analysis of phthalate metabolites in children based on sufficient number of samples and information about the site.

• 한국수정란이식학회지
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• 제22권2호
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• pp.81-87
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• 2007

본 연구에서는 수컷 랫드에 DEHP를 투여하여 실험적으로 생식 독성을 유발하고, vitamin E와 catechin을 단독 및 병용 투여하여 수컷 랫드에서 정소의 조직학적 변화, 정액 특성의 변화 및 정자의 운동성 변화 등을 조사하여 그 예방 효과를 알아보고자 하였다. DEHP를 투여한 군은 대조군에 비해 정자농도, 정자 생존율, 정상 정자율이 감소하였다. DEHP에 의한 생식 독성을 예방하기 위해 vitamin E와 catechin을 단독 또는 병행 투여한 결과, DEHP를 투여한 군에 비하여 정자 농도, 정자 생존율, 정상 정자율이 증가하였다. 이러한 결과를 종합해 볼 때, catechin과 vitamin E의 투여는 DEHP에 의한 생식 독성을 예방하는 효과가 있는 것으로 사료된다.

• 환경생물
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• 제22권2호
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• pp.321-328
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• 2004

The effects of Di (2-ethylhexyl) phthalate (DEHP) on the fourth instar larvae of Chironomus riparius were tested in the laboratory. Employing a waterreplacement exposure setup, chironomids were subjected to various concentrations. In the most treatments mortality reached a statistically significant difference from the control conditions. As DEHP concentrations were increased, the rates of emerged adults decreased. Sex ratio was unaffected with little deviation from a 1:1 relationship (except in 1 and $30\mu{g}\;L^{-1})$. The developmental stages was delayed at low concentration (0.3 and $1\mu{g}\;L^{-1})$. Generally the emergent period was different between males and females, and the first emergent day of males was faster than females. The body shape of female adults was larger than males. Differences between males and females were found in body volume, body length and body width. In addition, the body volume showed the significant difference between controls and treatments, and those especially well observed females.

• 공업화학
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• 제29권2호
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• pp.225-228
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• 2018

열가소성고분자에 첨가되는 가소제는 외부로의 용출로 인해 제품의 수명을 단축시키고, 일부는 환경, 인체에 유해한 영향을 미치는 것으로 알려져 있다. 발암물질로 의심되는 프탈레이트계 가소제의 사용이 점차 금지됨에 따라 이를 대체할 수 있는 친환경 가소제에 대한 필요성이 요구되며 대부분의 물질은 수지와의 상용성이 다소 떨어지거나 단가가 높으며 구체적인 안전성 검증이 부족한 문제가 있다. 따라서, 본 연구에서는 대체 가소제 후보물질로서 우수한 물리적 물성과 독성 평가에서 안전한 화합물로 보고되어진 노보넨 다이알킬에스터 화합물 4종을 PVC 수지에 첨가하여 용출에 의한 인체 노출 가능성을 평가하고 상용가소제 DEHP, DINCH, DOTP와 비교 분석하여 대체 물질로서의 가능성을 평가하고자 하였다. 시험은 american standard test method (ASTM) 규격에 준하여 시행하였으며 결과에 따르면, 수성 용매에서 di-2-ethylhexyl-5-norbornene-2,3-dicarboxylate (DEHN), 유성용매에서 diisopentyl-5-norbornene-2,3-dicarboxylate (DIPN) 화합물이 DEHP 대비 우수하거나 동등한 수치를 나타내었다. 또한 4종의 노보넨 화합물 모두 환경에 영향을 미칠 수 있는 수준으로 평가되는 100 ppm보다 낮은 수치를 나타내었으며 이는 극성부인 노보넨 구조가 용출성에 영향을 미친 것으로 보여진다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.403.3-404
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• 2002

Dimethyl phthalate(DMP). diethyl phthalate(DEP), di-n-butyl phthalate (DBP). butyl benzyl phthalate(BBP). bis(2-ethylhexyl)phthalate(DEHP) and di-n-octyl phthalate(DOP) in lotions was determined by gas chromatography. and benzyl benzoate was used as the intermal standard. The separation of the six phthalates and internal standard was optimized, and the optimal analytical conditions were as follows: column. DB-1701 (I.D. 0.25mm): mobile phase. helium: oven temperature 20$0^{\circ}C$ (10 min) ${\rightarrow}10^{\circ}C$/min ${\rightarrow}260^{\circ}C$/min(30min), injector temperature 23$0^{\circ}C$, detector temperature 28$0^{\circ}C$. (omitted)

• 환경생물
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• 제22권4호
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• pp.532-536
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• 2004

Due to the fourth-instar larvae of C. riparius have a sensitive to ecdysteroidal molting hormones for the life cycle developments, accordingly the emerged adult affected corresponding to larval phase's environments. The emerged female from larval phase exposure to DEHP observed a fact body and clumsy fling behavior in females. The body volume of treated female groups was clearly larger than that of control fe- males. In the 2D/E gel 1108 protein spots were identified. The visualized protein spots allowed extraction of 27 protein spots differed more than 3 fold in DEHP treated animals, which was approximately 2.4％ of the total protein spots. In this view, the body volume (or morphological characters) was well observed and detected faster than physiological detection for various EDCs. In this study, the body volume as a detecting po-int for EDCs suggested a bio-marker in individual levels.

• Molecular & Cellular Toxicology
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• 제3권4호
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• pp.299-305
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• 2007

The endocrine disrupting chemical, di (2-ethylhexyl) phthalate (DEHP) is a plasticizer used in polyvinyl chloride products ubiquitous in our daily lives. DEHP has potentially adverse effects on the liver, kidney, lung, heart, reproductive organs and endocrine systems. Many toxicological data on the DEHP toxicity have been stated, but complete protein profiles have not yet been reported. In this study, DEHP-induced oxidative DNA damage in rat lymphocyte was evaluated by Comet assay (single-cell gel electrophoresis) for the first time. Moreover, DEHP-induced protein profile alterations were examined in rat liver by using toxicoproteomic tools. 34 protein spots in the liver were identified to be significantly deregulated by DEHP on the 2-dimensional gel. Among them, 20 spots were up-regulated and 14 spots down-regulated by DEHP.