• Title/Summary/Keyword: dextrin$

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Mutation of a Bacillus stearothermophilus Strain for Over-production of Cyclomaltodextrin Glucanotransferase (Cyclomaltodextrin Glucanotransferase의 생산을 위한 Bacillus stearothermophilus 균주의 돌연변이)

  • 황진봉;김승호
    • Microbiology and Biotechnology Letters
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    • v.20 no.6
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    • pp.707-710
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    • 1992
  • Bacillus stearothermophilus No.239 isolated from soil was mutated with N-methyl-N'nitro-N-nitrosoguanidine (MNNG) to yield a series of mutants with increasing levels of cyclomalto-dextrin glucanotransferase (EC 2.4.1.19` CGTase) production. After five consecutive mautation steps, a mutant MNNG 8 with about 14 times of CGTase activity than the parent strain was obtained.

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Solubilization of poorly water-soluble drugs using solid dispersions

  • Kim, Tae-Wan;Choi, Choon-Young;Cao, Qing-Ri;Lee, Beom-Jin
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.412.2-413
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    • 2002
  • Purpose. To prepare polymer based physical mixtures or solid dispersions containing solubilizing compositions using a spray-dryer. Methods. Lovastatin.simvastatin.aceclofenac and cisapride were selected as poorly water-soluble drugs. Dextrin. poly(vinylalcohol). poly(vinylpyrrolidone)and polyethylene glycol were chosen as solubilizing carriers for solid dispersions. The solid dispersions containing solubilizing compositions without drug were prepared without using organic solvents or tedious changes of formulation compositions. (omitted)

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Effect of Pre-treatment Agents on the Digital Textile Printing of Silk Fabrics (견직물의 디지털 프린팅에 있어 전처리제가 염색성에 미치는 영향)

  • Lee, San-Ha;Jeong, Dong-Seok;Chun, Tae-Il
    • Textile Coloration and Finishing
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    • v.23 no.4
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    • pp.263-273
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    • 2011
  • Digital textile printing(DTP) technology made considerable advances in recent years. In this study, a pre-treatment agent has been prepared for the better coloration of digital textile printing. The ink formulation contained three kinds of 5g thickener (CMC, Sodium alginate, Dextrin), 25g urea, 5g sodium carbonate, and 465g distilled water. The optimal sharpness of outline was found in the 1-3% concentration of the pre-treating agent with a viscosity of 10-15 cSt. Even if the color difference between untreated and treated samples was not apparent in the printing step, the color appearance increased after steaming. The color appearance of cyan, magenta, yellow, black reactive colorants increased in the order of CMC>Sodium alginate>Dextrin. Wash fastness to shade change and staining for the treated samples were 4-5 rating, while untreated sample was 1-2 rating. Also, the pre-treated sample with 1:1 mixtures had 4-5 rating. Both dry and wet rubbing fastness to shade change and staining were excellent in the treated samples, whereas rubbing fastness of untreated sample was 1-2 rating. With exception of 3 rating to black color, light fastness properties were 4 rating for the remaining three colors in the regardless of treatment condition and mixing of pre-treating agents. Dry cleaning fastness of all samples were also 4-5 rating irrespective of treatment condition and mixing of pre-treating agents.

The Cultural Characteristics and Antibacterial Activities of Cordyceps militaris and Paecilomyces tenuipes (번데기동충하초와 눈꽃동충하초의 배양특성 및 항균활성)

  • Lee, Ki-Man;Hong, In-Pyo;Nam, Sung-Hee;Sung, Gyoo-Byung;Bae, Yoon-Hwan
    • Korean journal of applied entomology
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    • v.47 no.4
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    • pp.479-486
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    • 2008
  • The cultural characteristics and antibacterial activities of Cordyceps militaris and Paecilomyces tenuipes were compared. The mycelial growth was the highest on MCM (Mushroom Complete Medium) for C. militaris and on YMA (Yeast Malt Agar) for P. tenuipes. But the mycelial density on MMM (Mushroom Minimal Medium) was lower than other on media. The optimum mycelial growth was observed at $25^{\circ}C$. C. militaris was low mycelial growth when it was transferred over 5 times generation. The carbon source for the optimum mycelial growth was fructose of monosaccharide, maltose of disaccharide and dextrin of polysaccharide. The calcium nitrate of organonitrogen was found the best mycelial growth on C. militaris, while the sodium nitrate observed to be well for mycelial growth on P. tenuipes. The ammonium tartrate was observed to be the best among the inorganonitrogen used for mycelial growth. Antibacterial activities were found out just C. militaris against Bacillus cereus of Gram (+).

Preservation Methods of Xanthomonas campestris pv. oryzae in Relation to Virulence and Colony-Type Variation (벼 흰빛잎마름병균의 균주보관에 따른 병원성 및 집락변이현상)

  • Hwang In Gyu;Cho Yong Sup
    • Korean Journal Plant Pathology
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    • v.2 no.3
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    • pp.150-157
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    • 1986
  • Effects of presservation methods on viability and virulence of Xanthosmonas compestris pv. oryzae were investigated. The incidence of colony-type variants from freeze-dried and deep-frozen cultures was also determined. The suspending medium for freeze-dried cultures containing $10\%$ sucrose and $1\%$ gelatin showed the highest viability, and the virulence was well maintained in the suspending medium containing $2\%$ dextrin, $0.5\%$ ascorbic acid, 0.5% ammonium chloride, $0.5\%$ thiourea, and $0.85\%$ NaCl. Serially transferred cultures became attenuated. Rough colonies which had wrinkled surface occurred at a frequency of $1.9\%$ to $15.8\%$ during freeze-drying and freezing. The rough colonies consisted of nonseptated filamentous cells and rod-shaped cells. The virulence of rough colonies was weak as compared with the normal colony type.

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Studies of GanEiung (III) -Study on The Improvement of Decreasing Aroma of Insam(Ginseng) Gangjung and The Development of It′s Substitutional Materials (강정에 관한 연구(III) -인삼강정의 향 손실 개선과 대체 재료 개발에 관한 연구)

  • 이숙경;백남현
    • Journal of Food Hygiene and Safety
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    • v.16 no.3
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    • pp.227-231
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    • 2001
  • Basic materials for various making gangjung, various concentration 0∼25.0%(w/v) of coating agent and 0∼20.0% of substitutional materials carried out an experiment in sensory evaluation, expansion rate and hardness of substitutional materials. The results are as follows: 1. Added coating agent far improvement of decreasing aroma, arabic gum and dextrin significant from 20.0% to 25.0% compared with others. A good results flavor strength score and hedonic score of added 20.0% arabic gum are highest. 2. Expansion rate is caused by substitutional waxy rice, expansion rate decreased above 20.0% as tapioca above 5.0%, rice above 10.0%, brown waxy rice and wheat flour 15.0%. 3. After firing gangjung of substitutional materials, hardness increase concentration of substitutional materials. Therefore substitutional materials added to tapioca below 5.0%, rice and wheat flour 10.0%, brown waxy rice 15.0% is thought of good.

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Purification and Some Properties of Cyclodextrin Hydrolase (Cyclodextrin분해효소의 정제 및 그 특성)

  • Kim, Yong-Hwi;Shim, Kyu-Kwnag;Moon, Young-Hee
    • Applied Biological Chemistry
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    • v.33 no.1
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    • pp.79-86
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    • 1990
  • Cyclodextrin hydrolase from Bacillus stearothermophilus KFCC 21203 was purified and the properties of the purified enzyme were investigated. The enzyme was purified 15 folds with 77 % recovery by ammonium sulfate fractionation, DEAE-cellulose chromatography, and Ultro AcA 34 gel filtration. The specific activity and the molecular weight of the enzyme were 1.30 units/mg protein and about 29,500, respectively, The maximum activity of the enzyme was shown at $55^{\circ}C$ and pH 5.5. However, stable temperature and pH were $40^{\circ}C$ and $5.0{\sim}8.0$, respectively. The Km value for ${\gamma}-cyclodextrin$ was $3.78{\times}10^{-3}$ M. The degradation activity of the enzyme was selectively high for ${\gamma}-cyclodextrin$, and very low for ${\beta}-cyclodextrin$, but not for ${\alpha}-cyclodextrin$. The decomposed products of ${\gamma}-cyclodextrin$ were mainly glucose and maltose, and a little mlatotriose. The activity of the enzyme was very high for amylose, potato starch, corn starch, amylopectin and maltooligomer, and relatively high for glycogen and dextrin. The decomposed products of them were mainly glucose and maltose.

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Production of Powder Using Concentrated By-products of Grape Processing (포도박 농축액을 이용한 분말 제조)

  • Chang, Seog-Won;Shin, Nam-Sub;Song, Jeong-Hee;Park, Yong-Deok;Rho, Yong-Taek
    • Food Science and Preservation
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    • v.17 no.2
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    • pp.275-280
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    • 2010
  • By-products from grapes obtained during processing have been considered to be promising materialsfor various functional applications, and to have pharmaceutical properties. A grape powder was developed from a concentrate of by-products obtained during grape processing. As dextrin levels increased, the moisture content, L-, a-, and b- values all decreased, whereas sugar content generally increased. Catechin and resveratrol were detected in most samples, but quercetin was absent. Epicatechin and resveratrol levels either decreased or were not detected as dextrin concentration increased. These physiochemical properties indicate that a concentrate extracted using undiluted ethanol as solvent is optimal for industrial use.

Characterization of Vibrio ponticus KJS1 Isolated from Larvae of Maroon clownfish, Premnas biaculeatus (Maroon clownfish, Premnas biaculeatus 자어로부터 분리한 Vibrio ponticus KJS1의 특성)

  • Kim, Jong-Su;Rho, Sum;Kang, Bong-Jo
    • Journal of fish pathology
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    • v.20 no.1
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    • pp.25-31
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    • 2007
  • Vibriosis is one of the most important diseases affecting marin fish. In this study, a bacterium belonging to the Vibrio were isolated from maroon clownfish (Premnas biaculeatus) larvae (3~7 days after hatching). The bacterium was identified as Vibrio ponticus KJS1 by 16S rRNA gene sequence analysis. The result of biochemical test using the GN2 microplate (BioLog Inc, USA) showed that it has the ability to use 35 substrates including dextrin and sucrose was detected, could not degrade 52 substrates including α-cyclodextrin, and showed the weak ability to use 7 substrates including glycogen. It was sensitive to oxolinic acid, flumequine, ciprofloxacine, ofloxacin, norfloxacin, nalidixic acid, pefloxacin, and doxycycline hydrochloride.

Studies on the Physiological Characteristics of Bacterial Leaf Blight Pathogen of rice, Xanthomonas oryzae Dowson (벼 흰빛잎마름병균의 생리적 성상에 관한 시험)

  • Choi Yong Chul;Lee Kyung Whee;Cho Eui Kyoo
    • Korean journal of applied entomology
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    • v.10 no.2
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    • pp.97-101
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    • 1971
  • 1. The experiment was conducted to investigate the physiological characteristics on ten isolates of bacterial leaf blight pathogen of rice, Xanthomenas eryzae Dowson. Seven out of tin isolates were isolated from infected leaves of various rice varieties including IR strains in Korea. Isolates S-20 and S-103 were originated from IRRI in Philippines, and isolate H-5809 was allocated by National Institute of Agricultural Science in Japan. 2. All isolates Produced hydrogen sulfide and ammonia 9as from peptone sol. media, and reduced methylen blue, Gelatin liquefaction occurred by all isolates tested, although each isolate showed different degree of liquefaction No coagulation of the casein in milk by the isolates was observed, though some amount of acid production occurred in litmus milk by ail isolates tested. 3. All isolates utilized glucose and galactose, and slight utilization of esculin, mannitol, raffinose, salicin and saccharose was observed. Lactose, starch and dextrin, however, were not utilized at all by all isolates tested in the study.

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