• Journal of Embryo Transfer
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• v.20 no.1
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• pp.25-33
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• 2005

This study is a part of research that development of effective genetic resources preservation system using the in vitro spermatogenesis, in vitro insemination and culture system. We aimed for establishment of in vitro culture system with in vitro activated porcine oocytes. The porcine oocytes were matured for 48 hours in $TCM199+10\%$ FCS and activated with $7\%$ ethanol. The activated oocytes were cultured for 7 days in $TCM199+10\%$ FCS or $NCSU23+0.4\%$ BSA medium. The activated oocytes were not developed to the blastocyst stage in $TCM199+10\%$ FCS medium. However in $NCSU23+0.4\%$ medium, those were developed to blastocyst with $3\%$ of treated oocytes. We extended maturation duration of porcine follicular oocytes fur 48, 52, 56, 60, 64, 68, and 72 hours and activated with $7\%$ ethanol and cultured using $NCSU23+0.4\%$ BSA medium. The six percents of activated oocytes were developed to blastocyst in 48 hours and $10\%$ in 52 hours with comparatively low rates suggested to be not fully activated by regenerated MPF. Maturation durations from 56 hours to 68 hours supported to develop upto $11.9\~18.3\%$ of blastocysts. However the developmental rate was declined to $7.2\%$ at 72 hours of maturation duration because of cytoplasmic deterioration. The assumed time window for activation will be $56\~68$ hours of maturation duration. When the matured oocytes were activated with electric pulse of 1, 1.2, 1.4, 1.6, 1.8 and 2.0kV/cm for $80{\mu}s$, although appling the electric current once was not enough for activation, appling twice with 1.6kV/cm for $80{\mu}s$ was shown the highest developmental rate with $11.3\%$. When those were compared with activating methods, $15.7%$ of blastocyst rate was obtained in the $7\%$ ethanol. That was higher than those in electric pulse with $9.5\%$ and calcium ionophore method with $5.8\%$. In this experimental condition, the $7\%$ ethanol treatment was the most effective method for activating porcine oocytes.

• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.2
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• pp.215-221
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• 2011

To establish an indoor-rearing system for the Large Copper butterfly, $Lycaena$ $dispar$ and the Small Copper butterfly, $Lycaena$ $phlaeas$, the effect of temperature, photoperiod and host plants on larval development was investigated. The larvae of $Lycaena$ $dispar$ fed on $Rumex$ $crispus$, whereas the larvae of $Lycaena$ $phlaeas$ ate both $Rumex$ $crispus$ and $Rumex$ $acetosa$. The duration of the larval period of $Lycaena$ $dispar$ was 13.8 days on $R.$ $crispus$ and that, of $Lycaena$ $phlaeas$ was 15.9 days and 15.2 days on $R.$ $acetosa$ and $R.$ $crispus$ respectively. Laboratory experiments show that the Large Copper larvae are able to feed on other $Rumex$ species without harming their overall survival and can utilize these alternative host plants at least as efficiently as their natural host plant. This result suggests that plant chemistry is not responsible for their lack of utilization of the alternative host plants in the wild. Host plant choice by ovipositing females was measured with the two alternative hosts. $Lycaena$ $dispar$ preferred $R.$ $crispus$ to $R.$ $acetosa$, wheareas $Lycaena$ $phlaeas$ preferred $R.$ $acetosa$ to $R.$ $crispus$. Temperature has been proposed as an important determinant of developmental rate, lifespan and mortality in invertebrates. As temperature increased, length of the developmental period gradually decreased. The developmental period of the Large Copper larvae was 11.0 days and 28.5 days at $30^{\circ}C$ and $17.5^{\circ}C$, respectively.

• Korean Journal of Animal Reproduction
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• v.20 no.3
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• pp.239-250
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• 1996

The objective of the present experiments were to determine whether micromanipulative and electro-stimulation conditions for blastomere survival overlapped those for oocyte activation in porcine. Eggs selected for in vitro development potential of blastomeres isolated from 4-cell embryos and oocyte activation by electrostimulation were equilibrated for 5~10 min, in 0.3M sucrose solution containing 7.5$\mu\textrm{g}$/ml cytochalasin B, and then electrostimulated for 30$\mu$sec using one pulse of 100, 120, 150 or 180 volts DC with electrodes 0.2mm apart. Single blastomeres were inserted into empty zona pellucida prior to electrostimulaticn. Then they were cultured in 20${mu}ell$ drops of fresh BECM to observe their developmental ability in vitro in a humidified incubat or at 38.5$^{\circ}C$. The results obtained from these experiments are as follows : 1. When one pulse of 100, 120, 150 or 180 volts DC for 30$\mu$sec were applied to porcine oocytes having the slit formed on zona pellucida for activation, activation rates were 65.1, 66.7, 70.7 and 91.7%, respectively. Higher activation rate was observed in 180V. 2. Infact oocytes incubated for 30 min, in 0.3M sucrose solution after electrostimulation were significantally different from control group with increasing of voltages(p<0.05). When voltages used for electrostimulation were increased, activation rates of oocytes were improved in all treatment groups. 3. When zona punctured-oocytes were only electrostimulated, or incubated in 0.3M sucrose solution for 30 min. after electrostimulation at 180 volt DC, activation rates were 90.5 and 95.5%, respectively. And activation rates of zona punctured-oocytes were significantly different from the groups for which zona pellucida was not punctured(P<0.05). 4. When single blastomeres form 4-cell transferred into empty zona pellucida were incubated for 0, 15 and 30 min. in 0.3M sucrose solution after electrostimulation using one pulse of 180 volt DC for 30 $\mu$sec, developmental rates of electrostimulated-single blastomeres to blastocyst were 72.5, 59.0 and 51.2%, respectively, and the ratio of control group developed to blastocyst were 80.0%. 5. The average cell number in electrostimulated-blastomeres developed to blastocyst were 7.9~10.8, and reduced than the cell number in diploid control ; Also cell number decreased with increasing of voltages. The results of these experiments indicate that the optimal condition for achieving in vitro developmental ability of single 4-cell blastomeres and oocyte activatin is 1 pulse, duration 30 $\mu$sec. in 180 volt, and incubation of blastomeres and oocytes in 0.3M sucrose solution after electrostimulation was not significantally different from another treatment groups. The results also show that this condition is suitable for nuclear transplantation using porcine eggs.

• Therapeutic Science for Rehabilitation
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• v.10 no.2
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• pp.37-51
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• 2021

Objective : The purpose of this study was to analyze non-invasive treatments and drooling assessment methods in children with cerebral palsy and developmental disabilities, who drool. Methods : This study searched two hundred papers published in 2005-2019. Forty-four papers were selected based on their abstract and title, and ten papers were finally selected following a secondary search. Results : The PEDro Scale of the selected papers was high with an average of seven points. As a result of analyzing the overall trends, the study participants were primarily patients with cerebral palsy, and recently, the therapeutic intervention of oral sensory exercise was more actively studied than behavioral modification. Studies of behavioral modification and oral sensory exercise intervention methods were found to have differences in participant age and, cognitive level, number of participants, research design, treatment time, and duration. Studies to confirming the frequency and severity of the drooling measurement method were found to be the main factor. Conclusion : This study analyzed typical behavioral modification and oral sensory exercise interventions as examples of non-invasive therapeutic interventions for children with cerebral palsy and developmental disabilities and provided information to help select appropriate therapeutic intervention methods when planning non-invasive therapy using behavioral modification and oral sensory exercise therapy.

• Korean Journal of Animal Reproduction
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• v.14 no.1
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• pp.31-35
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• 1990

The viability and developmental potential of rat oocyte was assessed by the use of eosin B. Dye was prepared as a stock solution to contain 1.0 mg eosin B per ml of Brinster's Medium for Ovum Culture plus glucose (BMOC) -2 medium. Three dye concentrations were selected for the Oocytes from rat ovarian follicles were collected and assessed under various conditions of heat oocyte studies by dilution of the stock solution with BMOC: 0.12mM, 0.60mM and 1.20mM. treatment and preservation duration by dye exc¬lusion assay using eosin B. It is suggested that live oocyte excludes eosin B whereas dead oocyte does not.

• The Journal of Korean Physical Therapy
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• v.10 no.1
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• pp.53-65
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• 1998

The main purpose of this paper is to analyze the modes of therapeutic intervention. The emphasis is on the neurophysiological perpective arising out of neurological principles and developmental concepts. The obtained results are as follows. 1. The important hypostheses predicted that the group intervened by neurodevelopmental approach would improve motor function better than the group done by traditional approach and it was proved that neurodevelopmental approach was more effective in gross motor region(P<.01) 2. In the comparison of type of involovement, neurodevelopmental intervention group in spastic type showed improvements in the region of gross motor.(P<.001) 3. In the comparision of degree of disorder, neurodevelopmental intervention group showed improvement of motor function in all the gross motor region in the mild, moderate and severe case.(P<.001) 4. In the comparison of ages of intervention beginning, the group of child between 25-36, 49-60 and 61-72 months(P<.001) intervened by neurodevelopmental approach showed improvements of motor function. 5. In the comparison of intervention duration, neurodevelopmental intervention group showed improvements of motor function in gross motor region according to intervention durstion(P<.001)

• Development and Reproduction
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• v.1 no.2
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• pp.173-180
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• 1997

This study has been conducted to investigate the molting behavior and the effects of rearing temperature and day length on molting in the behavior and the effects of rearing temperature and day length on molting in the giant freshwater prawn, Macrobrachium rosenbergii reared in the laboratory. The results obtained were summarized as follow: 1. After pre-spawning molting, the protopodites of 1st, 2nd, 3rd, and 4th except 5th pleopod bore new breeding setae which conserve eggs in the brooding chamber and the basis of 3rd, 4th, and 5th pereiopods bore new breeding dresses which transport the ovulated eggs into the brooding chamber. 2. Adult females reared in 27.5-$29.4^{\circ}C$ molted 10-12 times per year at interval of 27-35 days, of which four or six moltings were common molting for growth and another four or five moltings were pre-spawning molting for spwaning and brooding. In winter season, pre-spawning molting did not happen to most of adult females in spite of the same temperature. 3. Duration of intermolt cycle was 31-38 days and 26-30 days at 25.3- $26.5^{\circ}C$ and 28.7- $30.4^{\circ}C$ of rearing temperature, respectively.

• Korean Journal of Animal Reproduction
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• v.19 no.3
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• pp.171-179
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• 1995

This study was carried out to investigate the fertilizing ability of round spematids isolated from seminiferous tubules. A round spermatid was introduced into the perivitelline space of a mature oocyte using Leitz micromanipulators and then subjected to electrofusion. Electrofusion was induced by applying a single DC pulse of 90V with a duration of 60$\mu$sec using Model 611 Square Wave Stimulator(Phipps and Bird, U.S.A) in 0.3 M sucrose fusion medium containing 0.05mM CaCl2 and 0.1mM MgSO4, Oocyte pre-activation was conducted by exposure to a single DC(80V, 80$\mu$sec) pulse in electrofusion medium at 1 hour before electrofusion. The incidence of fusion with pre-activated oocytes(23.8%, 57/239) was higher than that with nonactivated oocytes(6.7%, 3/45). The most of electro-stimulated mouse oocytes cleaved regardless of the success or failure of fusion. Karyotyping of embryos that developed into blastocysts after exposure to the fusion pulse were performe. We found that blastocysts from the fused oocytes were diploid whereas blastocysts from the unfused oocytes were haploid. About 11.7 and 11.5% of fused and unfused oocytes were developmental potentials of fused and unfused oocytes. Therefore, these results suggest that the mouse mture oocyte can be fertilized by fusion with a round spermtid and subsequently developed normally.

• Korean Journal of Biological Psychiatry
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• v.3 no.2
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• pp.149-155
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• 1996

Doctors who treat pregnant women ore usually cautious in writing their prescription for the drugs. The problem of which psychotropic medications ore sale during pregnancy seems to remain unsolved for many years. Although the rate of absorption is reduced due to a reduced rate of gastric emptying, the extent of absorption of drug is generally unchanged during pregnancy. Plasma volume and total body water increase during pregnancy. There is suggestion that drug metabolizing activity may be increased in pregnancy. Since the pregnancy increase the glomerular filtration rate significantly, drugs mainly eliminated by renal excretion will be cleared more quickly. Factors contributing to the potential teratogenecity of a drug include the type of compound, dose and duration of use, developmental stage of fetus at the time of exposure, and the effect of the drug on fetal pharmacokinetics. All major classes of psychotropic agents should be assumed to diffuse readily across the placenta to the fetus and to be present in some quantity in the breast milk. To decide when and how to start the drug treatment depends on an assessment of the risks related both with and without drug treatment of psychiatric disorders.

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.1
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• pp.21-25
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• 2003

The implications of temperature $(25, 30 and 35{\times}1^{\circ}C)$ and relative humidity $(60, 70 and 80{\times}2%)$ on the moulting pattern, moulting duration and moulting survival were studied in the silkworm, Bombyx mori L. Larvae of two pure silkworm breeds, Pure Mysore (PM) and NB$_4$D$_2$and their hybrid, $PM{\times}NB_{4} D_{2}$ were reared under experimental conditions under natural day photoperiodic (LD 12:12) condition. Two developmental marker events in the fourth moulting, settling for moult (SM) and completion of moult (CM) occurred at or around the middle of the photophase. The computed mean vector (equation omitted), based on the circular statistics also confirmed the above. Temperature and humidity did not alter the moulting rhythmicity much. However, extreme temperature and humidity conditions reduced moulting survival in PM and $PM {\times}NB_{4} D_{2}$. Further, moulting survival reduced below the economic level in $NB_{4} D_{2}$. The temperature and humidity together seem to exert synergic impact on the moulting survival of the silkworm Bombyx mori, at least in $NB_{4} D_{2}$.