• 한국동물생명공학회지
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• 제36권3호
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• pp.137-144
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• 2021

In this study, to analyze whether artificial regulation of apoptosis in the development of somatic cells can affect the stable growth and development of cells, 20 alpha-hydroxysteroid dehydrogenase (20α-HSD) and rapamycin were treated to induce apoptosis and autophagy in the both skin and muscle cells. Respectively, and 3-methyladenine was supplemented to inhibit cell death. Our results show that stimulation with rapamycin activated autophagy in both types of cells, but increased apoptosis more than autophagy in the case of skin cells. These results indicate that there was a difference in the expression of survival factors and cell development depending on the type of cell. In particular, in the expression of autophagy-related gene (MAP1LC3A) was higher than that of Casp-3, an apoptosis factor. Furthermore, cell development was the highest in all cell groups cultured by artificially inducing autophagy, however the lowest in the apoptosis-inhibited group. Especially, the noteworthy result of this study was that when apoptosis was induced using 20α-HSD, it was possible to induce apoptosis in both skin and muscle cells. Therefore, the main point of this study is that apoptosis induced during cell culture plays a pivotal role in cell remodeling.

• 한국가축번식학회지
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• 제17권4호
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• pp.287-294
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• 1994

This study was to investigate the effects of electrofusion, activation and developmental stage of donor embryos on in vitro development of nuclear transplant bovine embryos. A single blastomere nucleus from 8-cell to morula stage embryos produced by in vitro fertilization(IVF) was transferred into a recipient oocyte enucleated at 23∼25 h after in vitro maturation(IVM) or into a recipient oocyte enucleated and cultured for 14∼15 h. In one experiment the nuclear transplant embryos were subjected to additional activation treatments. Fusion rate of nuclear transplant eggs was high at direct current(D.C) voltages of 1.0 and 1.5 kV/cm 991.5 and 93.3%, respectively), but decreased at 2.0kV/cm (81.8%). Additional activation treatments by electric pulases or 7% ethanol did not affect the cleavage and development of nuclear transplant embryos. Development of nuclear transplant embryos slightly increased by delayed nuclear transfer and fusion (42∼43 h after IVM). With this system, blastocysts were obtained from transfer of 8-cell to morula stage donor nuclei (9.6%∼2.4%). The result of this study suggests that nucleo-cytoplasmic interactins, expecially activation of ooplast are very important for the development of nuclear transplant embryos, and donor cell stage does not affect the development of nuclear transplant embryos.

• 한국가축번식학회지
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• 제18권3호
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• pp.183-189
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• 1994

We examined early development in loach(Misgurnus mizolepis) embryos with parthenogenetic agents well-known in mammals. Female loach was superovulated with an intraperitoneal injection of 15 IU human chorionic gonadotrophin (hCG) per gram body weight. After 13 h of hCG injection, the oocytes were obtained from the abdomen. The oocytes were activated with 10% ethanol in tap water or fish Ringer's solution for 5, 10 and 15 minutes(eTW5, 10, 15 and eFRS5, 10, 15), respectively. The activation rates were 29% and 10% in eFRS10 and eFRS15, 5% and 6% in eTW10 and eTW15 by judging the cleaved blastomeres. Whereas, no parthenogenetic embryo was produced by tap water or fish Ringer's solution alone. The activation rate with the fish Ringer's solution was higher than that of tap water. No embryonic development was observed by calcium ionophore, A23187, at concentrations of 10, 20, 40 and 100$\mu$M when treated for 1, 2.5 and 5 minutes, respectively. The activation agents did not cause early development as in mammalian eggs. Therefore, the results suggest that fresh water fish may have a different egg activation pathway from that of mammals.

• 한국게임학회 논문지
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• 제16권1호
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• pp.73-82
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• 2016

스마트디바이스의 급속한 발전과 함께 디지털미디어적인 콘텐츠 소비속도가 매우 빨라지고 있다. 그로 인하여 다양한 이야기가 필요하게 되었고, 이야기를 창작하기 위해 문화 원형들의 재현이 많아지고 있다. 원형은 집단적 무의식 안에 내재된 행동이며, 상상과 경험에서 오는 전형적인 본보기인 만큼, 콘텐츠에 재현되는 원형이 왜곡되거나 변질되는 현상들은 민족 전통의 정체성까지도 위협할 수 있다. 본 논문은 원형 재현에 성공한 서양 콘텐츠와 원형재현에 실패한 국내 콘텐츠를 분석하고, 문화원형 재현의 실패에 따른 양상들을 파악하여, 향후의 스토리텔링 방법론을 고찰해 보았다.

• Journal of Plant Biology
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• 제28권1호
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• pp.45-55
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• 1985

홍조 Rhodochorton purpureum (Lightf.) Rosenvinge의 생식에 관한 연구를 일본 북해도산 식물을 중심으로 하여 수행하였다. 이 종은 주로 사분포자낭을 형성하지만 단포자낭은 자연상태나 실험실조건에서도 형성하지 않는다. Nemuro산 식물은 포자낭이나 배우자낭을 형성하지 않고 영양생식에 의한 번식을 하고 있다. 자연상태에서 영양생식은 사상체의 일부에 가근이 형성된 후에 그 부분이 떨어져나가 새로운 개체로 된다. Akkeshi와 Oshoro산(I) 식물에서 형성된 사분포자는 사분포자를 형성하는 식물체로 발달한다. 그러나 Muroran과 Oshoro산(II) 식물에서 형성된 사분포자는 배우체로 발달하는데, 이들 배우체는 사분포자낭과 웅성 또는 자성배우자낭을 형성하므로 배우체에 형성되는 사분포자낭은 유사분열에 의해서 형성되었으리라 생각한다.

• 한국작물학회지
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• 제44권2호
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• pp.171-175
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• 1999

Morphological alteration of floral organ development in rice affected by chilling stress was examined. Three varieties of rice were grown under natural conditions and subjected to 12$^{\circ}C$ for 3 or 6 days starting from the ineffective tillering stage, before heading stage and returned to natural condition. Headings were delayed by a 6 day chilling treatment. After heading the panicles were collected and examined for any possible alteration in floral organ development. It appears that there were some differences in sensitivity to chilling stress and degree of injury depending on treatment stages and variety. Chuchungbyeo was the most frequent in producing abnormal flowers among the three varieties examined. Meiosis stage was shown to be the most vulnerable to chilling stress in both Chuchungbyeo and Ilpumbyeo and young panicle differentiation stage was the frequent stage to alter flower development in response to chilling stress only in Chuchungbyeo. It was confirmed that abnormalities occurred in pollen due to chilling stress is a major factor leading to low yield, but to some extent the alterations in carpel development may playa certain role in determining a total yield in response to chilling stress at the reproduction stage in rice. There were abnormalities like extra stigmata, extra lemma, double ovary as well as abnormal anther formation in response to chilling stress. Further studies of the phenocopy observed in rice floral development may be useful for an understanding of the resistance against chilling injury during reproductive stages in rice.

• 한국인쇄학회지
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• 제24권1호
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• pp.23-34
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• 2006

Color reproduction is one of the most important expression factors in digital printing. In this paper, an experiment was done where the characteristics of color reproduction in digital printing. The results could summarized as follows. The printing used device profile showed a color difference of less than printing used default value in digital printing. As a result of weighting color difference, a difference between color gamut of digital original and color gamut of printing, when transforming the RGB color space to CMY color space, a exclusion the gray revision. The solution is to optimize the color transformation by gamut mapping and gray revision.

• 한국인쇄학회지
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• 제23권2호
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• pp.45-58
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• 2005

The propose of this study investigated the reproduction of color image displayed on a CRT monitor, for a range of different values of monitor gamma. We have used the GOG(gain-offset-gamma) model of the behavior of the CRT. Color difference have been computed in a color space, based on the CIELAB color appearance model. The 133 patch defined linearly color sample and 24 patch defined printing color target were used, and were subjected to the influence of nine different gamma value. The result show that neutral color is increasing the decrease range of luminance black color than white color. These results are of concern in the context of the "correct" display of color reproduction.

• 한국실내디자인학회논문집
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• 제23권4호
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• pp.157-168
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• 2014

In buildings on the street, indiscriminate redevelopment are being made by overlooking the relationship with surrounding landscape to create their own unique meaning, due to industrialization progressed rapidly with human needs of longing for something constantly. Selection and methods of two plans, conservation and utilization should be required, considering heritage values on industrial buildings which are being lost and aged in this redevelopment higher. The attempt for the use of modern industrial heritage becomes a hot topic, and several experimental challenges are being made. However, there are rather many cases of causing industrial buildings' depreciation and one-size-fits-all reproduction performance as it applies other examples as standards by recognizing the reproduction of modern industrial heritage as one trend, not focusing on the trend of the times or paradigm, what the times want. To minimize these problems, we focus on how to utilize industrial buildings beyond the importance of industrial buildings' reproduction, considering the quality of local residents' life as well as the expected effect obtainable due to security of local identity and reproduction of buildings, focusing on the concept of community design that offers and forms a way of community life as the design of shares based on unspecified individuals' common life. Community design's core can be called to have diversity rather than undifferentiated unity by pursuing whole unification, since local residents become those who plan design and all people participate in it. In addition, community design achieves integration with other fields as an enlarged design area without limiting in an environmental area, by making indigenously natural characteristics combined with cultural factors in one context so that people may understand the flow of the place. The importance of effects acquired from modern industrial heritage's reproduction has well-known, and the attempt for utilizing aged heritage as a new value and a function has constantly been made. Now, it's time to focus on how long it lasts through this method, rather than modern industrial heritage's reproduction itself. There can be a significance in this study in proposing methodological solutions on modern industrial heritage's reproduction, not to cause depreciation of industrial buildings by recognizing contemporary trends and uniform regeneration performance, multifaceted research is needed for creative, effective development on modern industrial heritage which will occur constantly.

• 한국가축번식학회지
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• 제8권2호
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• pp.110-115
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• 1984

These experiments were carried out to obtain the information about the optimal pH osmolality affecting in vitro fertilization of the mouse eggs, to elucidate the 2-cell block to development in vitro and to find out the method of controlling the subsequent embryo development in vitro. pH and osomlality was adjusted by adding NaCl or NaHCO3 to the basic salt solution. In vitro fertilization were carried out by inroducting the cumulus masses to the suspension of epididymal spermatozoa at each pH, osmolality, and 10${\mu}$M-EDTA medium. The results obtained in these experiments were summarized as follows: 1. The fertilization rates in vitro at each medium of 235, 252, 269, 286, 306, 323, 345, 368, 393 mosmol were 15.6, 38.2, 65.7, 75.6, 80.9, 74.3, 58.1, 35.1, 24.3, 11.1%, respectively. 2. The fertilization rates in vitro at each medium of pH 6.1, 6.4, 6.7, 7.0, 7.3, 7.6, 7.9, 8.1 were 11.8, 17.9, 32.4, 61.9, 79.5, 76.7, 53.5, 13.6%, respectively. 3. In case of ICR female x ICR male embryos, the development rate of 2-cell embryos to 4-8 cell embryos was 16.2% at normal medium, but the rate was increased to 49.3% in medium containging 10 ${\mu}$M-DETA; In case of C3H female x ICR male embryos, the development rate was 41.0% at normal medium, but the rate was increased to 71.7% at 10 ${\mu}$M-EDTA-medium.