• Journal of Microbiology
• /
• v.45 no.5
• /
• pp.453-459
• /
• 2007

We developed a multiplex PCR (mPCR) assay to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Corynebacterium spp. and seudomona aeruginosa. This method employs a single tube and multiple specific primers which yield 200, 281, 346, 423, 542, and 1,427 bp PCR products, respectively. All the PCR products were easily detected by agarose gel electrophoresis and were sequenced to confirm the specificity of the reactions. To test this method, DNA extracted from urine samples was collected from 96 sexually transmitted disease or prostatitis patients at a local hospital clinical center, and were subjected to the mPCR assay. The resulting amplicons were cloned and sequenced to exactly match the sequences of known pathogenic isolates. N. gonorrhoeae and Corynebacterium spp. were the most frequently observed pathogens found in the STDs and prostatitis patients, respectively. Unexpectedly, P. aeruginosa was also detected in some of the STD and prostatitis samples. More than one pathogen species was found in 10% and 80.7% of STD and prostatitis samples, respectively, indicating that STD and prostatitis patients may have other undiagnosed and associates. The sensitivity of the assay was determined by sing purified DNA from six pathogenic laboratory strains and revealed that this technique could detect pathogenic DNA at concentrations ranging from 0.018 to $1.899\;pg/{\mu}l$. Moreover, the specificities of this assay were found to be highly efficient. Thus, this mPCR assay may be useful for the rapid diagnosis of causative infectious STDs and prostatitis. useful for the infectious STDs and prostatitis.

• Korean Journal of Medicinal Crop Science
• /
• v.24 no.3
• /
• pp.237-245
• /
• 2016

Background: Cyanazine is used as a pre-emergent herbicide once during the growing season to control weeds of many upland crops worldwide. This study aimed to establish a method to determined cyanazine residue levels in major medicinal crops by using high performance liquid chromatography-UV detection/mass spectometry (HPLC-UVD/MS). Methods and Results: Cyanazine residue was extracted with acetone from the raw products of four representative medicinal plants - Scutellaria baicalensis, Paeonia lactiflora, Platycodon grandiflorum and Angelica gigas. The extract was diluted with a large volume of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. It was then purifined using optimized Florisil column chromatography. HPLC analysis conducted using an octadecylsilyl column allowed the successful separation of cyanazine from co-extractives of the samples, and the amount was sensitively quantified by ultraviolet absorption at 225 nm with no interference. The accuracy and precision of the proposed method were validated by conducting recovery experiments on each medicinal crop sample fortified with cyanazine at two concentration levels per crop in triplicate. Conclusions: The mean recoveries ranged from 91.2% to 105.3% for the four representative medicinal crops. The coefficients of variation were less than 10%, irrespective of the sample types and fortification levels. The limit of quantification of cyanazine was 0.02 mg/kg as verified by the recovery experiment. A confirmatory method was performed by liquid chromatography/MS using selected-ion monitoring technique to clearly identify the suspected residue.

• The Transactions of the Korea Information Processing Society
• /
• v.4 no.5
• /
• pp.1277-1288
• /
• 1997

In this paper,the extraciton method for individual and the recognition method for the printed dociments are discussed. In preprocessing is a technique to extract characters that are difficult to manage such as touching characters or overlapped chracters.Genrally in the existing segmentation methods,projection and edge detection are applied.However,in this paper an indvidual character is extracted by using connected pixel with one projection after the string extraction The maximum Blok Methld(MBM)is used for the recognition.The MBM is a method to enlarge the block to the last point the pixel that was found during projection. The maximum blocks are skeletonxied after the division into straight line block and oblique line block.Especially,in the recognition of chinese chracters compared to the existing method it showed improved recognition rate.

• Journal of Astronomy and Space Sciences
• /
• v.34 no.4
• /
• pp.315-330
• /
• 2017

Halo coronal mass ejections (CMEs) originating from solar activities give rise to geomagnetic storms when they reach the Earth. Variations in the geomagnetic field during a geomagnetic storm can damage satellites, communication systems, electrical power grids, and power systems, and induce currents. Therefore, automated techniques for detecting and analyzing halo CMEs have been eliciting increasing attention for the monitoring and prediction of the space weather environment. In this study, we developed an algorithm to sense and detect halo CMEs using large angle and spectrometric coronagraph (LASCO) C3 coronagraph images from the solar and heliospheric observatory (SOHO) satellite. In addition, we developed an image processing technique to derive the morphological and dynamical characteristics of halo CMEs, namely, the source location, width, actual CME speed, and arrival time at a 21.5 solar radius. The proposed halo CME automatic analysis model was validated using a model of the past three halo CME events. As a result, a solar event that occurred at 03:38 UT on Mar. 23, 2014 was predicted to arrive at Earth at 23:00 UT on Mar. 25, whereas the actual arrival time was at 04:30 UT on Mar. 26, which is a difference of 5 hr and 30 min. In addition, a solar event that occurred at 12:55 UT on Apr. 18, 2014 was estimated to arrive at Earth at 16:00 UT on Apr. 20, which is 4 hr ahead of the actual arrival time of 20:00 UT on the same day. However, the estimation error was reduced significantly compared to the ENLIL model. As a further study, the model will be applied to many more events for validation and testing, and after such tests are completed, on-line service will be provided at the Korean Space Weather Center to detect halo CMEs and derive the model parameters.

• Journal of Korean Society of Environmental Engineers
• /
• v.37 no.5
• /
• pp.293-302
• /
• 2015

Halonitromethanes (HNMs) are one of the most toxic groups of disinfection by-products. Recently, various studies have been fulfilled. An automated headspace-solid phase microextraction (SPME) gas chromatography/electron capture detector (GC-ECD) technique was developed for routine analysis of 9 HNMs in water samples. The optimization of the method is discussed. The limits of detection (LOD) and limits of quantification (LOQ) range from 90 ng/L to 260 ng/L and from 270 ng/L to 840 ng/L for 9 HNMs, respectively. Matrix effects in tap water and sea water were investigated and it was shown that the method is suitable for the analysis of trace levels of HNMs, in a wide range of waters. The method developed in the present study has the advantage of being rapid, simple and sensitive.

• Journal of the Institute of Convergence Signal Processing
• /
• v.5 no.4
• /
• pp.281-286
• /
• 2004

The wear process of end mill is a so complicated process that a more reliable technique is required for the monitoring and controling the tool life and its performance. This research presents a new tool wear monitoring method based on the sound signal generated on the machining. The experiment carried out continuous-side-milling for using the high-speed steel end mill under wet condition. The sound pressure was measured at 0.5m from the cutting zone by a dynamic microphone, and was analyzed at frequency domain. The tooth passing frequency appears as a harmonics form, and end mill wear is related with the first harmonic. It can be concluded from the result that the tool wear is correlate with the intensity of the measured sound at tooth passing frequency estimation of end mill wear using sound is possible through frequency analysis at tooth passing frequency under the given circumstances.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.22 no.2
• /
• pp.253-260
• /
• 2012

Target detection is very difficult with complex clutters in IRST(Infrared Search and Track) system for a long distance target. Especially sea-clutter and ocean-surface with non-uniform temperature distribution make it difficult to detect incoming targets in images obtained in sea environment. In this paper, we propose a novel method based on morphological method for estimation of ocean surface with non-uniform temperature flow. In order to estimate the exact ocean surface temperature flow, we divided it into upper and lower bound flow. And after estimating it, the final ocean surface temperature flow is derived by a mean value of the estimated results. Also, we apply the multi-weighted technique with a variety of sizes of structure elements to overcome sub-sampling effect by using morphology method. Experimental results for ocean surface images acquired from many different environments are compared with results of existing method to verify the performance of the proposed methods.

• Journal of Institute of Control, Robotics and Systems
• /
• v.12 no.5
• /
• pp.431-437
• /
• 2006

In this paper vector code correlation(VCC) method and an algorithm to promote the image-processing performance in building an effective measurement system using cameras are described far automatically berthing and controlling the ship equipped with side-thrusters. In order to realize automatic ship berthing, it is indispensable that the berthing assistant system on the ship should continuously trace a target in the berth to measure the distance to the target and the ship attitude, such that we can make the ship move to the specified location. The considered system is made up of 4 apparatuses compounded from a CCD camera, a camera direction controller, a popular PC with a built-in image processing board and a signal conversion unit connected to parallel port of the PC. The object of this paper is to reduce the image-processing time so that the berthing system is able to ensure the safety schedule against risks during approaching to the berth. It could be achieved by composing the vector code image to utilize the gradient of an approximated plane found with the brightness of pixels forming a certain region in an image and verifying the effectiveness on a commonly used PC. From experimental results, it is clear that the proposed method can be applied to the measurement system for automatic ship berthing and has the image-processing time of fourfold as compared with the typical template matching method.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.6 no.3
• /
• pp.33-42
• /
• 2003

As airborne laser scanning technique is developed with high vertical accuracy recently, there come many studies on DEM(digital elevation model creation, building extraction, flood risk mapping and 3D virtual city modeling. This study applied point comparative method, contour comparative method and digital map with scale 1/5,000 to calculate RMSE of DEM in according to resolution that was constructed using rawdata being acquired by airborne laser scanning. As a result, point comparative method showed lower DEM standard error than contour comparative method, it is a reason that contour comparative method was not carried out detailed grid calculation for point comparative method. Also, digital map with scale 1/5,000 showed higher DEM standard error than point comparative method and contour comparative method in below 25.4m that is average horizontal distance among contour line, and showed similar result with contour comparative method in over 25.4m.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.15 no.1
• /
• pp.172-183
• /
• 2012

In 2011, floods were at the worst stage of devastation in Chao Phraya river basin of Thailand. The purpose of this study is to trace the flood inundation area around Chao Phraya river basin by using Terra MODIS image because it has the ability of spatiotemporal dynamics. The MODIS indices, which included the enhanced vegetation index(EVI), land surface water index(LSWI), and the difference in the values of EVI and LSWI(DVEL), were extracted from MODIS product MOD09 8-day composite datasets with a spatial resolution of 500m from Jul. 29, 2011 to Jan. 09, 2012. We found that combined application of EVI, LSWI, and DVEL was suitable for monitoring flood inundation. For the extracted flood inundation area and water-related area. The result can be used to acquire the flood inundation data scattered and demonstrate the potential for the use of MODIS data for temporal and spatial detection of flood effects.