• The Plant Pathology Journal
• /
• v.27 no.1
• /
• pp.93-97
• /
• 2011

For quarantine purpose, we selected five plant RNA viruses including Cucumber vein yellowing virus (CVYV), Cucurbit yellow stunting disorder virus (CYSDV), Potato aucuba mosaic virus (PAMV), Potato yellow dwarf virus (PYDV), and Tomato chlorosis virus (ToCV), which are not reported in Korea and cause serious economic losses to the family Cucurbitaceae or Solanaceae. To detect those viruses, we employed RT-PCR technique with specific oligonucleotide primer pairs and tested their detection efficiency for each virus. To design RT-PCR primers, coat protein was used for CVYV, CYSDV, and ToCV whereas RNA polymerase and nucleocapsid regions were used for PAMV and PYDV, respectively. The development of an RT-PCR based method proved a useful tool for rapid detection and identification of quarantine virus infections.

• Parasites, Hosts and Diseases
• /
• v.25 no.1
• /
• pp.7-12
• /
• 1987

The indirect fluorescent antibody(IFA) test was used to detect serum IgG and IgM antibodies to Trichomonas vaginalis in 31 vaginal trichomoniasis, 7 candidiasis and in 20 non-infected healthy women with antigen prepared from axonic culture of Trichomenas vaginalis isolated from vulvovaginitis patient. The results were as follows: 1. In 31 vaginal trichomoniasis the positive reactions of IgG antibody were 27 in the 1/8 dilution or higher and :l in the 1/4 dilution whereas in healthy women the reaction showed significantly low as in the 1/4 dilution or below. 2. The sensitivity and specificity of IFA test for IgG antibody to trichomonad antigen in this study were 87.1% and 100%, respectively. 3. No significant difference of IgM antibody levels between vaginal trichomoniasis and healthy women was observed. 4. No relation between the levels of IgG and IsM antibodies to trichomonad antigen by IFA test was observed. 5. No relation between the time lapse and the level of serum IgG antibodies in IFA test of vaginal trichomoniasis was regarded. In conclusion the present study suggests that IFA test in trichomoniasis could be a useful tool for detection of anti-trichomonad IgG antibodies and applicable as an immunodiagnostic method.

• Parasites, Hosts and Diseases
• /
• v.49 no.3
• /
• pp.281-284
• /
• 2011

Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to promote public health. The present study compared several primer sets for small subunit (SSU) rDNA and histone genes of E. histolytica cysts. A 246 bp of the SSU rDNA gene of pure cysts contained in phosphate-buffered saline (PBS) and in stool samples was successfully amplified by nested PCR, using the 1,147-246 bp primer set, of the primary PCR products which were pre-amplified using the 1,147 bp primer as the template. The detection limit of the nested PCR using the 1,147-246 primer set was 10 cysts in both groups (PBS and stool samples). The PCR to detect histone gene showed negative results. We propose that the nested PCR technique to detect SSU rDNA can be used as a highly sensitive genetic method to detect E. histolytica cysts in stool samples.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.4 no.2
• /
• pp.43-58
• /
• 1986

In a mapping, stereo-restitution of an aerial photogrammetric process, of which is a major factor for map-base preparation dominates the accuracy and the reliability of a topographical map. The majority of a map-base preparation has nowadays been carried out by an analogue method ie, by the stereo-plotter. In consequence, it is evident that the skilled, the level of technique and personal attitude of operator have influence upon observational error which relates the accuracy and the quality of a map. This research aims at detection and analysis of operator's carrier and types of stereoplotter. The test is also examined that the level of details and features of terrain would have influence on the accuracy of map. With the results. it is also considered that the field check has impact on map accuracy ; whether the field check prior to restitution or after restitution.

• Korean Journal of Food Science and Technology
• /
• v.33 no.5
• /
• pp.521-524
• /
• 2001

A method using PCR was developed for the monitoring of glyphosate tolerant soybean (GTS) produced by the DNA recombination technique. We designed 3 pairs of specific oligonucleotide primers based on the gene sequences inserted in soybean and in lectin and ferritin genes as internal standards. Template DNAs were isolated from soybeans by the modified hexadecyl trimethyl ammonium bromide (CTAB)method and used for PCR with different primer sets. PCR, used with specific primer sets for GTS detection, showed the amplified DNA fragments with GTS template DNA but no product showed with non-GTS template. PCR amplified products were confirmed by DNA sequencing and were detected for up to 0.05% of GTS template DNA.

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
• /
• 2004.02a
• /
• pp.82-94
• /
• 2004

This paper addresses solutions to the challenges of carrier phase integer ambiguity resolution and cycle slip detection/identification, for maintaining high accuracy of an integrated GPS/Pseudolite/INS system. Such a hybrid positioning and navigation system is an augmentation of standard GPS/INS systems in localized areas. To achieve the goal of high accuracy, the carrier phase measurements with correctly estimated integer ambiguities must be utilized to update the system integration filter's states. The occurrence of a cycle slip that is undetected is, however, can significantly degrade the filter's performance. This contribution presents an effective approach to increase the reliability and speed of integer ambiguity resolution through using pseudolite and INS measurements, with special emphasis on reducing the ambiguity search space. In addition, an algorithm which can effectively detect and correct the cycle slips is described as well. The algorithm utilizes additional position information provided by the INS, and applies a statistical technique known as the cumulative-sum (CUSUM) test that is very sensitive to abrupt changes of mean values. Results of simulation studies and field tests indicate that the algorithms are performed pretty well, so that the accuracy and performance of the integrated system can be maintained, even if cycle slips exist in the raw GPS measurements.

• Journal of Korea Multimedia Society
• /
• v.10 no.2
• /
• pp.218-225
• /
• 2007

This paper presents a technique to detect a document skew that often occurs during document scanning. To correct a skewed document is essential for automatic processing system including character segmentation, character recognition and so on. The proposed algorithm can detect a skew angle exactly by searching characters baselines that have slant information of the document within a candidated area. To reduce processing time, we resized the image small and then established a ROI (region of interest) by morphology operations and connected components analysis. We compared our method with the existing method based on morphology operations and proved correctness and efficiency of the proposed algorithm through experiments and analysis with various kind of document images.

• KIPS Transactions on Computer and Communication Systems
• /
• v.1 no.1
• /
• pp.55-60
• /
• 2012

Recent botnets are widely using the DNS services at the connection of C&C server in order to evade botnet's detection. It is necessary to study on DNS analysis in order to counteract anomaly-based technique using the DNS. This paper studies collection of DNS traffic for experimental data and supervised learning for DNS traffic-based malicious domain classification such as query of domain name corresponding to C&C server from zombies. Especially, this paper would aim to determine significant features of DNS-based classification system for malicious domain extraction by the Principal Component Analysis(PCA).

• Korean Journal of Chemical Engineering
• /
• v.36 no.6
• /
• pp.975-980
• /
• 2019

Infrared (IR) spectroscopy is a powerful technique for observing organic molecules, as it combines sensitive vibrational excitations with a non-destructive probe. However, gaseous volatile compounds in the air are challenging to detect, as they are not easy to immobilize in a sensing device and give enough signal by themselves. In this study, we fabricated a thin nanocrystalline metal-organic framework (nMOF) film on a surface plasmon resonance (SPR) substrate to enhance the IR vibration signal of the gaseous volatile compounds captured within the nMOF pores. Specifically, we synthesized nanocrystalline HKUST-1 (nHKUST-1) particles of ca. 80 nm diameter and used a colloidal dispersion of these particles to fabricate nHKUST-1 films by a spin-coating process. After finding that benzene was readily adsorbed onto nHKUST-1, an nHKUST-1 film deposited on a plasmonic Au substrate was successfully applied to the IR detection of gaseous benzene in air using surface-enhanced IR spectroscopy.

• Korean Journal of Remote Sensing
• /
• v.22 no.5
• /
• pp.403-406
• /
• 2006

An algorithm for detection of yellow sand aerosols has been developed with infrared bands. This algorithm is a hybrid algorithm that has used two methods combined. The first method used the differential absorption in brightness temperature difference between $11{\mu}m\;and\;12{\mu}m\;(BTD1)$. The radiation at $11{\mu}m$ is absorbed more than at $12{\mu}m$ when yellow sand is loaded in the atmosphere, whereas it will be the other way around when cloud is present. The second method uses the brightness temperature difference between $3.7{\mu}m\;and\;11{\mu}m(BTD2)$. This technique is sensitive to dust loading, which the BTD2 is enhanced by reflection of $3.7{\mu}m$ solar radiation. First the Principle Component Analysis (PCA), a form of eigenvector statistical analysis from the two methods, is performed and the aerosol pixel with the lowest 10% of the eigenvalue is eliminated. Then the aerosol index (AI) from the combination of BTD 1 and 2 is derived. We applied this method to Multi-functional Transport Satellite-l Replacement (MTSAT-1R) data and obtained that the derived AI showed remarkably good agreements with Ozone Mapping Instrument (OMI) AI and Moderate Resolution Imaging Spectroradiometer (MODIS) aerosol optical depth.