• Title/Summary/Keyword: detection technique

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α -Cyclodextrin Modified Screen Printed Graphite Electrodes for Detection of Phenols

  • Kim, Hwa-jeong;Jang, Seung-Cheol;Shim, Yoon-Bo
    • Bulletin of the Korean Chemical Society
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    • v.23 no.3
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    • pp.427-431
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    • 2002
  • A screen printed graphite electrode has been developed for a simple and sensitive determination of phenolic compounds in an aqueous solution. The electrode developed uses a simple and effective screen printing technique with ${\alpha}-Cyclodextrin({\alpha}-CD)$ modified graphite ink. Phenols were captured on the surface of the ${\alpha}-CD$ modified electrode through complex formation. The phenol/ ${\alpha}-CD$ complex was deposited and quantified electrochemically using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV). The optimization of the experimental parameters was performed in regard to electrode composition, pH, temperature, sample preconcentration time. Interferences from other organic compounds were investigated. The detection limit for phenols was 500 ${\pm}7$ nM for DPV, with the linear range of 0.5 ${\mu}M$ -25.0 ${\mu}M$ and 30 ${\pm}2$ nM for SWV, with the linear range of 30 nM - $50{\mu}M$, respectively.

A comparison of RPLA and PCR for detection of enterotoxins in methicillin-resistant Staphylococcus aureus(MRSA) strains isolated in dogs

  • Park, Son-il;Han, Hong-ryul
    • Korean Journal of Veterinary Research
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    • v.39 no.4
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    • pp.806-810
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    • 1999
  • A multiplex-polymerase chain reaction (PCR) assay was used to detect staphylococcal enterotoxin production by 12 strains of Staphylococcus aureus isolated from clinical specimens. To evaluate the efficacy and/or sensitivity of this method, the results were compared to those obtained with the reversed passive latex agglutination kit (SET-RPLA, Denka Seiken, Japan). Of 10 strains positive by PCR were positive by RPLA but two strains, representing high sensitivity of the former method. Enterotoxin B was the most prevalent by the two methods. The kappa index between the two methods was 0.826, indicating a higher agreement and fully reliable for use. These results would suggest that sensitive, inexpensive, and relatively rapid multiplex-PCR technique may be an effective means for the detection of staphylococcal enterotoxin genes as an alternative to traditional methods such as kits or immunological methods, which depend upon the amount of enterotoxin produced.

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Detection of Impulse Signal in Noise Using a Minimum Variance Cepstrum -Application on Faults Detection in a Bearing System (최소 분산 캡스트럼을 이용한 노이즈 속에 묻힌 임펄스 검출 방법-베어링 결함 검출에의 적용)

  • 최영철;김양한
    • Journal of KSNVE
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    • v.10 no.6
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    • pp.985-990
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    • 2000
  • The signals that can be obtained from rotating machines often convey the information of machine. For example, if the machine under investigation has faults, then these signals often have pulse signals, embedded in noise. Therefore the ability to detect the fault signal in noise is major concern of fault diagnosis of rotating machine, In this paper, minimum variance cepstrum (MV cepstrum) . which can easily detect impulse in noise, has been applied to detect the type of faults of ball bearing system. To test the performance of this technique. various experiments have been performed for ball bearing elements that have man made faults. Results show that minimum variance cepstrum can easily detect the periodicity due to faults and also shows the pattern of excitation by the faults.

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Phytotoxins of Pseudomonas syringae and PCR Primers for Detection of Phytotoxin-Producing Strains (Pseudomonas syringae의 식물독소와 독소 생산 균주의 검출을 위한 PCR Primer)

  • 정재성;한효심;고영진
    • Research in Plant Disease
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    • v.7 no.3
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    • pp.123-133
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    • 2001
  • Many pathovars of the species Pseudomonas syringae are known to produce different phytotoxins as secondary metabolites. Although phytotoxins generally enhance the virulence of P. syringae, they are not required for pathogenesis. Among the phytotoxins produced by P. syringae, lipodepsipeptides, coronatine, phaseolotoxin, and tabtoxin are the most well-known toxins which have been intensively studied for their structure, mode of action, biosynthesis, and regulation. A polymerase chain reaction (PCR) technique that amplifies a segment of the phytotoxin gene cluster using a primer set has been developed in recent years. This method offers the advantages of speed and sensitivity compared to the approaches based on physiological and biochemical methods. PCR detection of genes involved in the production of toxins could be exploited for early diagnosis of plant diseases caused by P. syringae pathovars.

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Measurement of Strain of Sheet Metal by Image Processing (II) (화상처리기법을 이용한 판재의 변형률 측정(II))

  • 정재윤;황창원;허만성;김낙수
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1997.10a
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    • pp.147-152
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    • 1997
  • This paper presents a method for measuring and analyzing strains in sheet metal forming by stereo vision system. A better understanding and evaluation of the formability can be achieved by analyzing stain distribution in deformed sheet metals. By means of image processing technique, it is possible to get strain distribution efficiently. For measuring strain automatically and accurately, algorithms for camera calibration, grid detection, stereo matching and strain analysis has been developed and verified by experiments with GUI(Graphic User Interface).

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Mercury ion detection technique using KPFM (KPFM을 통한 수은이온 검출 방법)

  • Park, Chanho;Jang, Kwewhan;Lee, Sangmyung;You, Juneseok;Na, Sungsoo
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2014.10a
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    • pp.358-360
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    • 2014
  • For the several decades, various nanomaterials are broadly used in industry and research. With the growth of nanotechnology, the study of nanotoxicity is being accelerated. Particularly, mercury ion is widely used in real life. Because the mercury is representative high toxic material, it is highly recommended to detect the mercury ion. In previous reported work, thymine-thymine mismatches (T-T) capture mercury ion and create very stable base pair ($T-Hg^{2+}-T$). Here, we performed the high sensitive sensing method for direct label free detection of mercury ions and DNA binding using Kelvin Probe Force Microscope (KPFM). In this method, 30 base pairs of thymine (T-30) is used for mercury specific DNA binding ($T-Hg^{2+}-T$). KPFM is able to detect the mercury ion because there is difference between bare T-30 DNA and mercury mediated DNA ($T-Hg^{2+}-T$).

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Calibration of Detection System of Crack in Concrete Structure by Using Image Processing Technology

  • Kim, Su-Un;Shin, Sung-Woo;Park, Jeong-Hak;Choi, Man-Yong
    • Journal of the Korean Society for Nondestructive Testing
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    • v.31 no.6
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    • pp.626-634
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    • 2011
  • The investigation of concrete structure typically relies on visual inspection which is one of the basic inspection techniques. Image processing techniques play a crucial role in the growing field of automatic surface inspection technique. However, kinds of inspection equipment, environmental condition and detection algorithm have much influence on the reliability of inspection result. This paper proposes a verification method and testing procedure for the reliability of inspection results and surveys characteristics of image acquisition systems and crack inspection algorithms.

Deadlock Detection using Graph Technique in Puzzle Game Environment (퍼즐 게임 환경에서 그래프 기법을 이용한 교착상태의 발견)

  • Park, Moon-Kyoung;Choi, Yong-Suk
    • Proceedings of the Korean Information Science Society Conference
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    • 2011.06c
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    • pp.343-346
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    • 2011
  • 대부분의 퍼즐 게임에서 발생 할 수 있는 중요한 문제 중 하나는 교착상태 문제이다. 본 논문에서는 이러한 교착상태를 해결하기 위해 퍼즐 게임을 그래프 기법으로 나타낸 뒤, 이를 이용한 새로운 교착상태 발견 기법인 Cycle Detection을 제안한다. 기존의 기법들은 알고리즘을 수행하는데 너무 많은 시간이 걸리거나, 패턴에 대한 데이터베이스가 구축되어 있어야 하기 때문에 실시간으로 교착상태를 발견하기엔 문제가 있다. 본 논문에서는 이러한 문제점을 해결하기 위하여 탐색해야 하는 노드의 개수를 최대한 줄이는 Local search 기법과 Pruning 기법을 적용하여 퍼즐 게임을 플레이하는 동안 실시간으로 교착상태를 발견할 수 있는 기법을 제안한다. 본 기법은 성능을 평가하기 위해 실제 퍼즐게임 환경에서 알고리즘을 수행하고, 그 결과로 검색하는 노드의 개수와 검색 시간을 기존의 기법과 비교하여 성능향상을 확인하였다.

Measurement of Brillouin Backscattering for Distributed Temperature Sensor Applications

  • Kim, Su-Hwan;Kwon, Hyung-Woo;Kwon, Hyun-Ho;Jang, Hang-Seok;Kim, Jee-Hyun;Kang, Shin-Won
    • Journal of Sensor Science and Technology
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    • v.20 no.1
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    • pp.8-13
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    • 2011
  • We present measurements of the Brillouin frequency shift in an optical fiber using a 1550 nm distributed feedback laser diode(DFB-LD) as a light source. By modulating the probe light with an electro-optic modulator, we confirm the stimulated Brillouin gain spectrum(BGS) and spontaneous BGS using the coherent detection method. We also confirm the applicability of the technique to distributed temperature sensors that measure the change in Brillouin frequency shift due to temperature variations.

A study on the development of CW(Continuous-Wave) Doppler System using FFT (FFT를 이용한 연속초음파 도플러 장치에 관한 연구)

  • Lee, Dae-Hyung;Kang, Chung-Shin;Park, Sei-Hyun;Kim, Young-Kil
    • Proceedings of the KIEE Conference
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    • 1988.07a
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    • pp.709-712
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    • 1988
  • Ultrasonic Doppler Diagnostic System utilizes the Doppler effect for measurement of blood velocity. The sign of the Doppler frequency shift represents blood flow direction. CW(Continuous-Wave) Doppler System uses quadrature detection and phase rotation method to produce simultaneous independent audio and velocity signals for forward and reverse blood flow direction in the time-domain, had been fabricated. But time-domain analyzing such as audio evaluation and zero- crossing detection for instantaneous and mean frequnecy measurement do not provide both an accurate and quantitative result. Therefore, it is necessary to adopt frequency-domain technique to improve system performance. In this paper, we describe a unit which is composed of CW Doppler System and real-time spectrum analyzer (installed TMS 32010 DSP Chip). This unit shows time-dependent spectrum variation and mean velocity of Blood signal.

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