• Title/Summary/Keyword: detection technique

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Rapid Detection of Serum HCV RNA by Combining Reverse Transcription and PCR without RNA Extraction

  • Jang, Jeong-Su;Lee, Kong-Joo
    • Archives of Pharmacal Research
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    • v.19 no.6
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    • pp.486-489
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    • 1996
  • A simple, rapid, specific and sensitive method for the detection of serum hepatitis C virus (HCV) RNA using the reverse transcription-polymerase chain reaction (RT-PCR) technique without conventional RNA extraction was developed. HCV template RNA from serum was obtained by boiling the serum at $95^{\circ}C$ for 2 min, cooling rapidly in ice and removing the proteins by cetrifugation. RT-PCR amplifications including the reverse transcription and first PCR amplification were performed in one vessel containing both of reverse transcriptase and Taq DNA polymerase. The detection of HCV RNA from $10^{-3}{\mu}l$. serum was possible with this method. The suitability of this method for clinical analysis was evaluated by assaying HCV RNA in 225 patient samples including anti-HCV antibody negatives (13 samples) and positives (212 samples) by enzyme-linked immunosorbent assay test (ELISA). Detections of HCV RNA with this method were in 4 of 13 anti-HCV antibody negative samples (30.8%) and 95 of 212 positive samples (44.8%). The present method can be completed in 1 hr and has a wide range of application for the clinical utilities to determine the viral RNAS.

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A Generalized Blind Adaptive Multi-User Detection Algorithm for Multipath Rayleigh Fading Channel Employed in a MIMO System

  • Fahmy Yasmine A.;Mourad Hebat-Allah M.;Al-Hussaini Emad K.
    • Journal of Communications and Networks
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    • v.8 no.3
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    • pp.290-296
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    • 2006
  • In this paper, a generalized blind adaptive algorithm is introduced for multi-user detection of direct sequence code division multiple access (OS-COMA) wireless communication systems. The main property of the proposed algorithm is its ability to resolve the multipath fading channel resulting in inter symbol interference (ISI) as well as multiple access interference (MAI). Other remarkable properties are its low complexity and mitigation to the near-far problem as well as its insensitivity to asynchronous transmission. The proposed system is based on the minimization of the output energy and convergence to the minimum mean square error (MMSE) detector. It is blind in the sense that it needs no knowledge of the other users' signatures, only the intended user signature and timing are required. Furthermore, the convergence of the minimum output energy (MOE) detector to the MMSE detector is analytically proven in case of M-ary PSK. Depicted results show that the performance of the generalized system dominates those previously considered. Further improvements are obtained when multiple input multiple output (MIMO) technique is employed.

Advanced Feature Selection Method on Android Malware Detection by Machine Learning (악성 안드로이드 앱 탐지를 위한 개선된 특성 선택 모델)

  • Boo, Joo-hun;Lee, Kyung-ho
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.30 no.3
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    • pp.357-367
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    • 2020
  • According to Symantec's 2018 internet security threat report, The number of new mobile malware variants increased by 54 percent in 2017, as compared to 2016. And last year, there were an average of 24,000 malicious mobile applications blocked each day. Existing signature-based technologies of malware detection have limitations. So, malware detection technique through machine learning is being researched to detect malware variant. However, even in the case of applying machine learning, if the proper features of the malware are not properly selected, the machine learning cannot be shown correctly. We are focusing on feature selection method to find the features of malware variant in this research.

High-Performance Liquid Chromatographic Determination of Cyclosulfamuron Residues in Soil, Water, Rice Grain and Straw

  • Lee, Young-Deuk;Kwon, Chan-Hyeok
    • Korean Journal of Environmental Agriculture
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    • v.23 no.4
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    • pp.251-257
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    • 2004
  • Analytical methods were developed to determine cyclosulfamuron residues in soil, water, rice grain and straw using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. In these methods, cyclosulfamuron was extracted with aqueous $Na_2HPO_4$/acetone and acetone/methanol mixture from soil and rice samples respectively. Liquid-liquid partition coupled with ion-associated technique, Florisil column chromatography, and solid-phase extraction (SPE) were used to separate cyclosulfamuron from interfering co-extractives prior to HPLC analysis. For water sample, the residue was enriched in $C_{18}$-SPE cartridge, cleaned up in situ, and directly subjected to HPLC. Reverse-phase HPLC under ion-suppression was successfully applied to determine cyclo-sulfamuron in sample extracts with the detection at its ${\lambda}_{max}$ (254 nm). Recoveries from fortified samples averaged $87.8{\pm}7.1%$ (n=12), $97.3{\pm}7.2%$ (n=12), $90.8{\pm}6.6%$ (n=6), and $78.5{\pm}6.7%$ (n=6) for soil, water, rice grain and straw, respectively. Detection limits of the methods were 0.004 mg/kg, 0.001 mg/L, 0.01 mg/kg and 0.02 mg/kg for soil, water, rice grain and straw samples, respectively.

Efficient Signal Detection Technique Using Orthogonal Sequence for Quantum Communication (직교 시퀀스를 이용한 양자통신에서의 효율적인 신호 검출 기법)

  • Kim, Yoon-Hyun;Kim, Jin-Young
    • Journal of Satellite, Information and Communications
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    • v.7 no.1
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    • pp.21-26
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    • 2012
  • For the last 20 years, our country has been pointing to a great power for digital information technology, but quantum information technology which is already researched in many forefront nations lags significantly behind other countries. Recently, quantum information management, quantum computing and quantum communication based on the quantum mechanics have been researching actively in many fields such as cryptology. On the basis of these background, in this paper, to efficient data transmission and detection for quantum data, we apply the orthogonal sequence to quantum communication system. The performance of proposed scheme is analyzed in terms of auto and cross correlation performance.

Ultrasonic Flaw Detection in Composite Materials Using SSP-MPSD Algorithm

  • Benammar, Abdessalem;Drai, Redouane
    • Journal of Electrical Engineering and Technology
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    • v.9 no.5
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    • pp.1753-1761
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    • 2014
  • Due to the inherent inhomogeneous and anisotropy nature of the composite materials, the detection of internal defects in these materials with non-destructive techniques is an important requirement both for quality checks during the production phase and in service inspection during maintenance operations. The estimation of the time-of-arrival (TOA) and/or time-of-flight (TOF) of the ultrasonic echoes is essential in ultrasonic non-destructive testing (NDT). In this paper, we used split-spectrum processing (SSP) combined with matching pursuit signal decomposition (MPSD) to develop a dedicated ultrasonic detection system. SSP algorithm is used for Signal-to-Noise Ratio (SNR) enhancement, and the MPSD algorithm is used to decompose backscattered signals into a linear expansion of chirplet echoes and estimate the chirplet parameters. Therefore, the combination of SSP and MPSD (SSP-MPSD) presents a powerful technique for ultrasonic NDT. The SSP algorithm is achieved by using Gaussian band pass filters. Then, MPSD algorithm uses the Maximum Likelihood Estimation. The good performance of the proposed method is experimentally verified using ultrasonic traces acquired from three specimens of carbon fibre reinforced polymer multi-layered composite materials (CFRP).

Detection of Genus Phytophthora and Phytophthora cryptogea-P. drechsleri Complex Group Using Polymerase Chain Reaction with Specific Primers

  • Hong, Seung-Beom;Park, In-Cheol;Go, Seung-Joo;Ryu, Jin-Chang
    • The Plant Pathology Journal
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    • v.15 no.5
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    • pp.287-294
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    • 1999
  • A technique based on the polymerase chain reaction (PCR) for the specific detection of genus Phytophthora and Phytophthora cryptogea-P. drechsleri complex group was developed using nucleotide sequence information of ribosomal DNA (rDNA) regions. The internal transcribed spacers (ITS) including 5.8S were sequenced for P. cryptogea-P. drechsleri complex group and its related species. Two pairs of oligonucleotide primers were designed. Primer pair ITS1/Phy amplified ca. 240 bp fragment in 12 out of 13 specie of Phytophthora, but not in Pythium spp., Fusarium spp.and Rhizoctonia solani. Primer pair rPhy/Pcd amplified 549 bp fragment only in P. cryptogea-P. drechsleri complex group, but not in other Phytophthora spp.and other genera. Specific PCR amplification using the primers was successful in detecting Phytophthora and P. cryptogea-P. drechsleri complex group in diseased plants.

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Imaging of a Defect in Thin Plates Using the Time Reversal of Single Mode Lamb Wave: Simulation

  • Jeong, Hyun-Jo;Lee, Jung-Sik;Bae, Sung-Min;Lee, Hyun-Ki
    • Journal of the Korean Society for Nondestructive Testing
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    • v.30 no.3
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    • pp.261-270
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    • 2010
  • This paper presents an analytical investigation for a baseline-free imaging of a defect in plate-like structures using the time-reversal of Lamb waves. We first consider the flexural wave (A0 mode) propagation in a plate containing a defect, and reception and time reversal process of the output signal at the receiver. The received output signal is then composed of two parts: a directly propagated wave and a scattered wave from the defect. The time reversal of these waves recovers the original input signal, and produces two additional side bands that contain the time-of-flight information on the defect location. One of the side band signals is then extracted as a pure defect signal. A defect localization image is then constructed from a beamforming technique based on the time-frequency analysis of the side band signal for each transducer pair in a network of sensors. The simulation results show that the proposed scheme enables the accurate, baseline-free detection of a defect, so that experimental studies are needed to verify the proposed method and to be applied to real structure.

A Hybrid Digital Watermarking Technique for Copyright Protection and Tamper Detection on Still images (정지영상에서 저작권 보호 및 위변조 검출을 위한 하이브리드 디지털 워터마킹 기법)

  • Yoo Kil-Sang;Song Geun-Sil;Choi Hyuk;Lee Won-Hyung
    • Journal of Internet Computing and Services
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    • v.4 no.4
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    • pp.27-34
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    • 2003
  • Digital image manipulation software is now readily available on personal computers. It is therefore very simple to tamper with any image and make it available to others. Therefore. copyright protection of digital contents and insurance of digital image integrity become major issues. In this paper, we propose a hybrid watermarking method to identify locations of tampered region as well as copyright. Our proposed algorithms embed the PN-sequence into low frequency sub-band of the wavelet transform domain and it doesn't need the original image in extraction procedure. The experimental results show good robustness against any signal processing with tamper detection on still image.

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Detection of Gamma-Irradiated Grains by Using DEFT/APC Method (곡류의 감마선 조사 검지를 위한 DEFT/APC 방법의 이용)

  • Oh, Kyeung-Nam;Lee, Sook-Young;Yang, Jae-Seung
    • Korean Journal of Food Science and Technology
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    • v.34 no.3
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    • pp.380-384
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    • 2002
  • For the screening of gamma-irradiated grains, domestic rice, glutinous rice, barley, and wheat were irradiated with 0.1, 0.3, 0.5, 0.7, and 1.0 kGy and screened using the DEFT/APC (Direct Epifluorescent Filter Technique/Aerobic Plate Count) method. The log DEFT/APT units increased with the dose increment in all samples, whereas the log APC unit decreased gradually. For rice, barley, and wheat, unirradiated and irradiated samples with below 0.3 kGy had 2.0 or lower logarithmic units, whereas those with 0.5 kGy or higher had 2.0 or higher logarithmic units. For glutinous rice, the sample irradiated with 0.5 kGy showed 1.92 logarithmic unit and those with 0.7 kGy or higher had 2.0 or higher logarithmic units. These results suggest that if the grains show 2.0 or higher logarithmic units, they could be assumed to have been irradiated at a dose level of at least 0.5 kGy. In conclusion, grains could be easily screened through the DEFT/APC method.