• Title/Summary/Keyword: detection technique

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PAX1 Methylation Analysis by MS-HRM is Useful in Triage of High-grade Squamous Intraepithelial Lesions

  • Wang, Zhen-Ming
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.2
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    • pp.891-894
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    • 2014
  • This study is aimed to investigate the role of paired boxed gene 1 (PAX1) methylation analysis by methylation-sensitive high-resolution melting (MS-HRM) in the detection of high grade lesions in atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (ASC-H) and compared its performance with the Hybrid Capture 2 (HC2) human papillomavirus (HPV) test. In our study, 130 cases with a diagnosis of ASC-H from the cervical cytological screening by Thinprep cytologic test (TCT) technique were selected for triage. Their cervical scrapings were collected and evaluated by using PAX1 methylation analysis (MS-HRM) and high-risk HPV DNA test (HC2), followed by colposcopy and cervical biopsy. Chi-square test were used to test the differences of PAX1 methylation or HPV infection between groups. In the detection of CIN2+, the sensitivity, specificity, the PPV, NPV and the accuracy of PAX1 MS-HRM assay and high-risk HPV (HR-HPV) tests were respectively 80.6% vs 67.7%, 94.9% vs 54.5%, 83.3%, vs 31.8%, 94.0% vs 84.4%, and 91.5% vs 57.7%. The PAX1 MS-HRM assay proved superior to HR-HPV testing in the detection of high grade lesions (CIN2+) in ASC-H. This approach could screen out the majority of high grade lesion cases of ASC-H, and thus could reduce the referral rate to colposcopy.

Self-Reference PCSR-G Method for Detecting Defect of Flat Panel Display (평판 디스플레이 결함 검출을 위한 자기 참조 PCSR-G 기법)

  • Kim, Jin-Hyung;Lee, Tae-Young;Ko, Yun-Ho
    • Journal of Korea Multimedia Society
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    • v.18 no.3
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    • pp.312-322
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    • 2015
  • In this paper a new defect detection method for flat panel display that does not require any separately prepared reference images and shows robustness against problems with regard to pixel tolerance and nonuniform illumination condition is proposed. In order to perform defect detection under any magnification value of camera, the proposed method automatically obtains the value of pattern interval through an image analysis. Using the information for pattern interval, an advanced PCSR-G method presented in this paper utilizes neighboring patterns as its reference images instead of utilizing any separately prepared reference images. Also this paper proposes a scheme to improve the performance of the conventional PCSR-G method by extracting and applying additional information for pixel tolerance and intensity distribution considering the value of pattern interval. Simulation results show that the performance of the proposed method utilizing pixel tolerance and intensity distribution is superior to that of the conventional method. Also, it is proved that the proposed method that is implemented using parallel technique based on GPGPU can be applied to real system.

Development of Low-Cost Vision-based Eye Tracking Algorithm for Information Augmented Interactive System

  • Park, Seo-Jeon;Kim, Byung-Gyu
    • Journal of Multimedia Information System
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    • v.7 no.1
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    • pp.11-16
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    • 2020
  • Deep Learning has become the most important technology in the field of artificial intelligence machine learning, with its high performance overwhelming existing methods in various applications. In this paper, an interactive window service based on object recognition technology is proposed. The main goal is to implement an object recognition technology using this deep learning technology to remove the existing eye tracking technology, which requires users to wear eye tracking devices themselves, and to implement an eye tracking technology that uses only usual cameras to track users' eye. We design an interactive system based on efficient eye detection and pupil tracking method that can verify the user's eye movement. To estimate the view-direction of user's eye, we initialize to make the reference (origin) coordinate. Then the view direction is estimated from the extracted eye pupils from the origin coordinate. Also, we propose a blink detection technique based on the eye apply ratio (EAR). With the extracted view direction and eye action, we provide some augmented information of interest without the existing complex and expensive eye-tracking systems with various service topics and situations. For verification, the user guiding service is implemented as a proto-type model with the school map to inform the location information of the desired location or building.

Detection of Influenza A Virus by Interdigitated Nanogap Devices

  • Park, Jimin;Park, Dae Keun;Lee, Cho Yeon;Kang, Aeyeon;Yun, Wan Soo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.419-419
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    • 2014
  • Interdigitated nanogap device (IND) is an attractive tool for biomolecular detection due to its huge on-off signal ratio, great tolerance to the variation in biochemical environment, and relatively simple implementation processes. Here, we report on the IND-based detection of Influneza A virus by sandwich immunoassay. The INEs were fabricated by photo lithography followed by the in-house chemical lithographic technique for the narrowing the initial gap distance. The surface of the silicon oxide between the two gold electrodes was chemically modified to immobilize primary antibodies for the immuno-specific interaction with the influenza A virus antigen. After immersing the functionalized-IND into the sample solution containing the influenza A virus, the device was exposed to the secondary antibody conjugated Au nanoparticles (Au NPs). The INDs showed a huge jump in the electric conductance when the sample solution contained the influenza A virus of the concentration as low as 10 ng/mL. We hope that this IND-based sensing can be applied to the development of simple and reliable diagnostic means of influenza viruses.

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PCR-Based Sensitive Detection of Wood-Decaying Fungus Phellinus linteus by Specific Primer from rDNA ITS Regions

  • Park, Dong-Suk;Kang, Hee-Wan;Kim, Ki-Tae;Cho, Soo-Muk;Park, Young-Jin;Shin, Hye-Sun;Lee, Byoung-Moo;Go, Seung-Joo
    • Mycobiology
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    • v.29 no.1
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    • pp.7-10
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    • 2001
  • Based on the rDNA ITS sequences data, specific primer set for PCR detection of wood-decaying fungus Phellinus linteus was designed. The length of PCR products using designed primer set(SHF and SHR) was about 540 bp. Among 11 species, 17 isolates of Phellinus spp. including Phellinus linteus, P. pomaceus, P. spiculosus, P. baumi, P. pini, P. igniarius, P. gilvus, P. biscuspidatus, P. weirii, P. johnsonianus, P. robutus, and P. igniarius, seven isolates of Phellinus linteus showed about 540 bp-sized single band. This molecular technique could offer a useful tool for detecting and identifying Phellinus linteus.

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Image Enhancement using Weighted Cross-Shaped Moving Window Median Filter (가중 격자형 메디안 필터를 이용한 영상향상)

  • Kim, Su-Yeong;Lee, Seung-Sang;Kang, Seong-Jun;Na, Cheol-Hun
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2013.05a
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    • pp.807-810
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    • 2013
  • In this paper, a new technique for image enhancement using weighted cross-shaped median filter with edge-detection algorithm is proposed. It consists of simple hypothesis test for edge-detection, and makes use of the cross-shaped moving window. This method is applied to noise corrupted image and its results are compared with those of median filters. As for the experimental result, method of weighted cross-shaped median filter is superior to other median filters.

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A Soft Output Enhancement Technique for Spatially Multiplexed MIMO Systems (공간다중화 MIMO 시스템을 위한 Soft Output 성능향상 기법)

  • Kim, Jin-Min;Im, Tae-Ho;Kim, Jae-Kwon;Yi, Joo-Hyun;Cho, Yong-Soo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.9C
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    • pp.734-742
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    • 2008
  • In spatially multiplexed MIMO systems that enable high data rate transmission over wireless communication channels, the spatial demultiplexing at the receiver is a challenging task and various demultiplexing methods have been developed. Among the previous methods, maximum likelihood detection with QR decomposition and M-algorithm (QRM-MLD), sphere decoding (SD), QOC, and MOC schemes have been reported to achieve a (near) maximum likelihood (ML) hard decision performance. In general, however, the reliability of soft output of these schemes is not satisfactory. In this paper, we propose a method which enhances the reliability of soft output. By computer simulations, we demonstrate the improved performance by the proposed method.

Determination of Volatile Organic Compounds (VOCs) Using Tedlar Bag/Solid-phase Microextraction/Gas Chromatography/Mass Spectrometry (SPME/GC/MS) in Ambient and Workplace Air

  • Lee, Jae-Hwan;Hwang, Seung-Man;Lee, Dai-Woon;Heo, Gwi-Suk
    • Bulletin of the Korean Chemical Society
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    • v.23 no.3
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    • pp.488-496
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    • 2002
  • SPME techniques have proven to be very useful tools in the analysis of wide VOCs in the air. In this study, we estimated VOCs in ambient and workplace air using a Tedlar ba /SPME/GC/MS system. The calibration curve was set to be linear over the range of 1-30 ppbv. The detection limits ranged from 10 pptv to 0.93 ppbv for all VOCs. Reproducibility of TO-14 target gas mixtures by SPME/GC/MS averaged at 8.8 R.S.D (%). Air toxic VOCs (hazardous air pollutants, HAPs) containing a total of forty halohydrocarbons, aromatics, and haloaro-matic carbons could be analyzed with significant accuracy, detection limit and linearity at low ppbv level. Only reactive VOCs with low molecular weight, such as chloromethane, vinylchloride, ethylchloride and 1,2-dichloro-ethane, yielded relatively poor results using this technique. In ambient air samples, ten VOCs were identified and quantified after external calibration. VOC concentration in ambient and workplace air ranged from 0.04 to 1.85 ppbv. The overall process was successfully applied to identify and quantify VOCs in ambient/workplace air.

A Study on the Constitution and the Application of FIA System for Measurement of Nitrite (The Field Water Samples at Kwangju) (아질산성질소 축정용 FIA의 제작 및 용용에 관한 연구 (광주광역시 광주천 시료를 대상으로))

  • Rhee, J.S.;Park, W.C.;Lee, S.W.;Kim, Y.J.
    • Journal of Korean Society on Water Environment
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    • v.18 no.3
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    • pp.283-290
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    • 2002
  • In this study, home-made detection system by means of noble FIA is introduced on the measurement of toxic nitrite in the water samples collected from the area of Kwangju. As the standard calibration between 30 to 1000 ppb, the linearity has been shown more than 0.9999 as the correlation coefficient($R^2$) with the detection limit 1.5 ppb(S/N>2). The distribution of sample concentration was monitored as N.D. - 123 ppb which is wide span of concentrations in field water samples. The low level of nitrite is hardly detectable with other expensive sophisticated instruments including ion chromatography. Whereas the result of high concentration brings forth the necessity monitoring constantly our precious water resources. Successfully, the FIA system has played a very important role detecting wide span of nitrite in water sample. This technique can be adopted for controlling our environment in the near future.

Research on the Security of Infrastructures Using fiber Optic ROTDR Sensor (광섬유 ROTDR센서를 이용한 사회기반시설물의 보안에 관한 연구)

  • Park, Hyung-Jun;Koh, Kwang-Nak;Kwon, Il-Bum
    • Journal of the Korean Society for Nondestructive Testing
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    • v.23 no.2
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    • pp.140-147
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    • 2003
  • A detection technique is studied to determine the location and the weight of an intruder into infrastructure using fiber optic ROTDR (Rayleigh optical time domain reflectometry) sensor. Fiber optic sensing plates buried in sand are prepared to measure the intruder effects. The signal of ROTDR was analyzed to confirm the detection performance. The constructed ROTDR system could be used up to 12km at the pulse width of 30ns. The location error was less than 3m and the weight could be detected into three levels of grade, such as 20kgf, 40kgf and 60kgf.