• KSBB Journal
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• v.29 no.2
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• pp.118-123
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• 2014

It was confirmed that malodor connected with an air-conditioner in an automobile is caused by microbial volatile organic compounds (MVOCs) produced by microorganisms getting into an air-conditioner when it is operating. Chemicals such as hydrogen sulfide, dimethyl sulfide, nbutyric acid, n-valeric acid, iso-valeric acid, n-octanol and toluene were detected above the odor threshold inside the automobile. The characteristics of a funky odor in the air blown into the automobile were due to detected sulfur compounds (hydrogen sulfide and dimethyl sulfide). Dimethyl sulfide was produced by microorganisms such as Aspergillus versicolor, Methylobacterium aquaticum, Herbaspirillum sp. and Acidovorax sp. In addition, the characteristics of a sour odor in the air blown into the automobile were due to detected organic acids (n-butyric acid, n-valeric acid and iso-valeric acid). N-valeric acid and iso-valeric acid were generated from Aspergillus versicolor, while iso-valeric acid was produced by Methylobacterium aquaticum. In addition, the odor intensity of the air blown into the automobile was affected by the concentration of detected sulfur compounds and organic acids. On the other hand, it is estimated that chemicals such as hydrogen sulfide, n-octanol and n-butyric acid detected in the air blown into the automobile were produced by non-identified species of microorganisms.

• The Plant Pathology Journal
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• v.22 no.1
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• pp.11-15
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• 2006

Five seed samples of carrot were tested to detect Alternaria spp. by blotter method. A. alternata and A. radicina were detected from all the seed samples as high as $25.8-70.5\%$ and $37.5-63.5\%$, respectively. A. dauci was detected from four seed samples as low as $0.5-7.5\%$. The three Alternaria spp. were detected from the pericarp and the seed coat and endosperm of the carrot seeds but not from the embryo by component plating test. A. alternata and A. radicina were much more detected from the pericarp than the seed coat and endosperm. A. dauci was detected from the pericarp and the seed coat and endosperm at similar rate. The seed sample which was most severely infected with A. radicina showed the lowest rate of germination in the test on top of paper (TP). In the TP test, differences in total infection rate of A. radicina and A. dauci of the seed samples were very closely correlated with those in incidence of seedling rot on the seed samples. However, there was no correlation between infection rate of A. alternata and rate of germination or seedling rot of the seed samples. Soil test for seedling growth revealed that there was no correlation between differences in total infection rate of A. radicina and A. dauci and those in rate of normal seedlings of the seed samples.

• Korean Journal Plant Pathology
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• v.11 no.4
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• pp.367-373
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• 1995

The cDNAs derived from the coat protein (CP) genes of six plant RNA viruses, tobacco mosaic virus-pepper strains (TMV-P) and -ordinary strain (TMV-OM), potato virus Y (PVY), turnip mosaic virus (TuMV), cucumber mosaic virus (CMV) and potato leafroll virus (PLRV), were subcloned into the transcription vector, pSPT18, containing SP6 and T7 promoters. The digoxigenin (DIG)-labeled RNA polymerase after linearlization of the cloned pSPTs with XbaI or SacI, and were tested for their sensitivities for the detection of the six viruses. In slot-blot hybridization, dilution end points for the detection of TMV-P and TMV-OM were 10-4, while those of PVY, TuMV and CMV were 10-3. PLRV was detected at the dilution of 10-2. When each RNA probe was applied for the detection of the viruses in the preparations from the leaf disks (8 mm in diameter, and 12 to 15 mg in weight) of infected natural host plants, TMV-P, TMV-OM and TuMV could be detected from one disk, while PVY from 1 or 2 disks. CMV was detected in the preparation from two disks, and PLRV from three disks. With DIG-labeled RNA probe, PVY was detected at 5 days after inoculation, but with ELISA the virus was detected at 8 days after inoculation to tobacco (Nicotiana tabacum cv. Xanthi nc) plants on which symptoms appeared at 9 days after inoculation. No difference was observed in cross reaction between the RNA probes for the detection of TMV-P and TMV-OM.

• Ocean and Polar Research
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• v.24 no.4
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• pp.391-398
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• 2002

Trace organic contaminants in deep-sea sediments near Dokdo were analyzed. Total PAMs concentration ranged 14.8-314 ng/g dry weight and high molecular weight PAHs were dominant. The highest PAHs concentration was detected at A19 which located at Ulleung Basin. Most of organochlorines were under detection limit. Among the detected organochlorines, DDT compounds were dominant and followed by HCHs and HCB. Butyltin compounds and most of organophosphorus pesticides were not detected. Vertical distribution of PAHs showed typical sub-surface maximum and decreasing trends depending on depth. The highest PAHs concentration reached 454ng/g. Some organochlorines, DDT, HCH was detected and also showed decreasing trends. Other target organic pollutants were not detected in core sediments. Abnormally high level of PAHs concentration in A19 was discussed and the input sources were inferred to be the transport of sludge derived pollutant dumped at dumping site 'Byung' by deep current.

• Korean Journal of Veterinary Research
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• v.38 no.2
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• pp.239-245
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• 1998

The distributions and relative frequencies of insulin-, glucagon-, and somatostatin-immunoreactive cells in the pancreas of the duck(Anas platyrhynchos platyrhyncos, Linne) were investigated immunohistochemically on 23 days of incubation, at hatching, 1 week, 2 weeks, 3 weeks, 5 weeks, 6 weeks, 7 weeks, 9 weeks, 10 weeks, and 32 weeks after hatching. In the duck pancreas on 23 days of incubation and at hatching, mammalian type islets(mixed type) were only observed, thereafter three type's islets(mamalian, A and B type's islets) were identified. Insulin-immunoreactive cells were detected in central region of the islets, while glucagon- and somatostatin-immunoreactive cells were detected in marginal region of light(B type) or mammalian type islets, and in central region of dark islets(A type). Insulin-, and somatostatin-immunoreactive cells were also detected in the exocrine regions. In this region the insulin-immunoreactive cells were detected from 23 days of incubation to 6 weeks, however not detected after 7 weeks. At hatching the relative numbers of somatostatin-immunoreactive cells were more frequent than those of other groups, and then decreased with ages.

• Journal of the Optical Society of Korea
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• v.14 no.4
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• pp.376-382
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• 2010

We propose a novel method to precisely detect car license plates by locating main characters, which are printed with large font size. The regions of the main characters are directly detected without detecting the plate region boundaries, so that license regions can be detected more precisely than by other existing methods. To generate a binary image, multiple thresholds are applied, and segmented regions are selected from multiple binarized images by a criterion of size and compactness. We do not employ any character matching methods, so that many candidates for main character groups are detected; thus, we use a neural network to reject non-main character groups from the candidates. The relation of the character regions and the intensity statistics are used as the input to the neural network for classification. The detection performance has been investigated on real images captured under various illumination conditions for 1000 vehicles. 980 plates were correctly detected, and almost all non-detected plates were so stained that their characters could not be isolated for character recognition. In addition, the processing time is fast enough for a commercial automatic license plate recognition system. Therefore, the proposed method can be used for recognition systems with high performance and fast processing.

• The Journal of the Korean Society for Microbiology
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• v.21 no.2
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• pp.205-210
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• 1986

Serological investigations for the leptospirosis on hospitalized patients in Choonchun Sungsim Hospital during the periods from August to November 1985 and 841 inhabitants of Kangwondo area including Choonchun, Choonsung, Inje, Chulwon, Hwachun, Gosung, Taibaik, Samchuk and Yangju area were carried out. 1. Among 58 hospitalized patients who were suspected as leptospirosis, 10 patients were detected to have antibody against Leptospira. All of positive sera had the highest antibody titer against serogroup Icterohemorrhagiae and most positive sera were also reactive to serogroup Australis and Canicola. Antibody titer of positive sera detected by microscopic agglutination(MA) test were ranging from 1 : 40 to 1 : 2,560. Antibody titer detected by ELISA method were higher than those detected by MAT(ELISA 1 : 400$\sim$1 : 25,600) and IgM titer of positive sera were generally higher than IgG titer. 2, Of 841 inhabitants in 8 area of Kangwondo, 17 persons (2,02%) possessing antibody against Leptospira were detected by ELISA method, IgG titer in positive sera were generally higher than IgM titer. Persons possessing antibody to Leptospira were distributed in both sex and in various age group, and no significant regional and occupational fluctuations were obserbed.

• Natural Product Sciences
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• v.8 no.2
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• pp.35-43
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• 2002

Glycosides of herbal medicines, such as glycyrrhizin, ginsenosides, kalopanaxsaponins, rutin and ponicirin, were studied regarding their metabolic fates and pharmacological actions in relation to intestinal bacteria using germ-free, gnotobiotic and conventional animals. When glycyrrhizin (GL) was orally administered, $18{\beta}-glycyrrhetinic\;acid\;(GA)$, not GL, was detected in plasma and intestinal contents of gnotobiotic and conventional rats. However, GA could not be detected in germ-free rats. When GL was incubated with human intestinal bacteria, it was directly metabolized to GA (>95%) or via $18{\beta}-glycyrrhetinic\;acid-3-{\beta}-D-glucuronide$(>5%). Orally administered GL was effective in gnotobiotic and conventional rats for liver injury induced by carbon tetrachloride, but was not effective in germ-free rats. When ginseng saponins were orally administered to human beings, compound K in the plasma was detected, but the other protopanxadiol saponins were not detected. The compound K was active for tumor metastasis and allergy. When kalopanaxsaponins were incubated with human intestinal microflora, they were metabolized to kalopanaxsaponin A, kalopanaxsaponin I and hederagenin. These metabolites were active for rheumatoid arthritis and diabetic mellitus while the other kalopanxsaponins were not. When flavonoid glycosides were orally administered to animals, aglycones and/or phenolic acids were detected in the urine. The metabolic pathways proceeded by intestinal bacteria rather than by liver or blood enzymes. These metabolites, aglycones and phenolic acids, showed antitumor, antiinflammatory and antiplatelet aggregation activities. These findings suggest that glycosides of herbal medicines are prodrugs.

• Journal of Magnetics
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• v.18 no.2
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• pp.173-177
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• 2013

Cantilever-detected high-frequency electron spin resonance (ESR) is a powerful method of sub-terahertz and terahertz ESR spectroscopy for a tiny magnetic sample at low temperature. In this technique, a small magnetization change associated with ESR transition is detected as deflection of a sample-mounted cantilever. So far, we have succeeded in ESR detection at 370 GHz using a commercial piezoresistive microcantilever. The spin sensitivity was estimated to ${\sim}10^{12}$ spins/gauss. In order to further increase the sensitivity, we adopt a fiber-optic-based detection system using a Fabry-Perot interferometer in place of piezoresistive system. Fabry-Perot cavity is formed between an optical-fiber end and microcantilever surface, and a change in the interference signal, corresponding to the cantilever deflection, is sensitively detected. This system is suitable for low-temperature and high-magnetic-field experiments because of its compact setup and less heat dissipation. In this study, performance of Fabry-Perot interferometer is evaluated, and its application to cantilever-detected ESR measurement is described.

• Journal of the East Asian Society of Dietary Life
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• v.10 no.1
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• pp.62-70
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• 2000

This study was conducted to investigated the kimchi quality of the addition of green tea extract and green tea leaf. Kimchi was examined for pH, titratable acidity, the changes in the number of microorganism, color,sensory evaluation . The pH and titratable acidity of kimchi at the addition of 5%, 1% of green tea extract and green tea leaf were higher and lower than those of control kimchi. the number of total microoranism at 21 days. were detected much more in the kimchi added green tea extract and green tea leaf than in control. The number of coliforms up to 7 days of fermentation were detected. And those at 14-21 days were not detected but those of 28 days were detected. The number of coliforms at 28 days were most lowest in the kimchi added green tea extract 3%. The number of lactic acid bacteria were detected more in the kimchi added green tea leaf. As a result of sensory evaluation during fermentation, the kimchi added green tea extract 5% was the highest score in carbonated taste. texture and overall quality. As the result of correlation between sensory characteristics and mechanical characteristics, it was found that sensory characteristics of sour taste is negatively related to the mechanical characteristics of L value, while it is positively related to the texture. The a values is negatively related to the sensory characteristics of overall quality. The b values is positively related to the sensory characteristics of sour taste.