• Journal of Food Hygiene and Safety
• /
• v.38 no.3
• /
• pp.123-130
• /
• 2023

Salmonella spp. are prevalent foodborne pathogens that are infective at relatively low concentrations, thus posing a serious health threat, especially to young children and the elderly. In several countries, the management and regulation of Salmonella spp. in food, including seafood, adhere to a negative detection standard. The risk of infection is particularly high when seafood is consumed raw, which underscores the importance of timely detection of pathogenic microorganisms, such as Salmonella. Accordingly, this study aimed to develop a combined pre-treatment and detection method that includes pre-culture and DNA extraction in order to detect five species of Salmonella at concentrations below 10 CFU/mL in seafood. The effectiveness of the proposed method was assessed in terms of the composition of the enrichment (pre-culture) medium, minimum incubation time, and minimum cell concentration for pathogen detection. Furthermore, a practical DNA extraction method capable of effectively handling high salt conditions was tested and found to be successful. Through polymerase chain reaction, Salmonella spp. Were detected and positively identified in shellfish samples at cell concentrations below 10 CFU/g. Thus, the proposed method, combining sample pre-treatment and cell culture with DNA extraction, was shown to be an effective strategy for detecting low cellular concentrations of harmful bacteria. The proposed methodology is suitable as an economical and practical in situ pre-treatment for effective detection of Salmonella spp. in seafood.

• The Korean Journal of Zoology
• /
• v.13 no.3
• /
• pp.68-74
• /
• 1970

In the previous studies, the present author found that high proportion of the follicular oocytes from mouse and rabbit ovaries are able to resume their maturation division in the anterior chamber of the eye in which they have been incubated by auto- or homoplastic transplantation. Especially in the case of the homoplastic transplantation, it was known that no trouble has been detected in the process of resumption of the oval maturation in particular connection with the antigen-antibody reaction between donor and recipient. These findings provide a possibility that the follicular oocytes from various animals would be matured in the eye even after the xenoplastic transplantation. Under such an assumption, the present studies were performed to examine the behavior of the follicular oocytes in the eye chamber of the animals of different species. For the donor of the follicular oocytes, domestic rabbits, albino rats of Sprague-Dowley strain, and albino mice of A-strain bred in our laboratory were used. The oocytes obtained from the ovarian follicules were introduced to the anterior chamber of the eye of different species of animals, with an exception of rabbit in which only the female animals were used as a recipient. The procedures of collection of ova, introduction to the eye, harvest from the eye ball, fixation, and staining were the same as mentioned in the previous reports (Cho, 1967b; Cho and Kim, 1968). The conclusions obtained are summarized as below. 1. The rabbit follicular oocytes are able to mature in the eye chambers of both male mouse and rat, although the proportion of the maturation is lower than when they are incubated autoplastically in the eye. When the ova were incubated in the male mouse eye for 24 hours, 21 per cent of them showed chromosomes at metaphase I and II, whereas the rate was 32 per cent when they were incubated in the eye of the male rat. These are apparently low comparing to the rate of 52 per cent of autoplastic transplantation. 2. When rat follicular oocytes were transferred into the mouse eye chamber and recovered after 24 hours, 43 per cent of them produced the mataphase I and II chromosomes. This proportion was higher than the result of the homoplastic transplantation which yielded 23 per cent of the ova on maturation. 3. The most striking result was found in the experiment with mouse follicular oocytes. Seventy-six per cent of the oocytes resumed their maturation division within 24 hours after they were transferred into the male rat eye chamber, and this figure was significantly high compared to the result o 55 per cent obtained by the homoplastic transplantation. In the rat eye, the induction of the degenerative ova also was low (19%). On the other hand, the proportion of the oval maturation decreased to 45 per cent, while that of degeneration increased 33 per cent when they were incubated in the eye of the female rabbit. 4. It was apparent from the present experiments that the follicular oocytes can reveal their activation to maturation in the eye chamber which contains aqueous humor which is known to be composed of low protein content and of very little gamma-globulin which acts as an antibody(Oser, 1965), and that it shows higher osmolarity than blood serum(Levene, 1958). Taking these properities into consideration the humor may provide unfavourable environment to the cells and tissues incubated in. However, it could be noteworthy finding that only the follicular oocytes in the eye of the different species can grow in healthy condition although the maturation rates are varied with the animal species. The fact that the rabbit follicular oocytes show the lower proportion in maturation may be due to the greater amount of the yolk granules in the egg cytoplasm than those in the mouse and rat oocytes. That the mouse oocytes incubated in the eye of the rat mouse and rat oocytes. That the mouse oocytes incubated in the eye of the rat resumed their maturation process in greater proportion would e explained by the fact that the rat eye chamber particularly provides the better environment to the mouse oocytes than the eye chamber of mouse does.

• Journal of Korea Entertainment Industry Association
• /
• v.13 no.7
• /
• pp.375-384
• /
• 2019

The purpose of this study was to investigate the effects of long-term aerobic dancing exercise on body composition, physical fitness and mental function in older women over 70 years old. All subjects were limited to older women aged 70 to under 85. The experimental group performed aerobic exercise of 2-3 times a week for 9.2 ± 4.6 yrs, and the control group did not perform any regular exercise and spent 3-4 times a week mainly in the relaxing facility for older. As a results in this study, the weight, muscle mass and body fat percentage of the aerobic group were not significantly different from those of the control group, but there were significantly positive effects of aerobic group in visceral fat mass, abdominal obesity rate(%), body water content and systolic blood pressure(p < .05). In terms of physical fitness, Sit & reach, one-legged stand (right) and TUG of the aerobic group were significantly higher than those of the control group(p < .05), but there were no significant differences between two groups in the shoulder flexibilities and grip strength. MMSE as a cognitive function was not significantly different between the two groups, but GDS was significantly lower in the aerobic group than in the control group(p < .05). According to the correlation analysis between all variables and MMSE and GDS, MMSE was significantly inversely correlated with height (r = -0.425)(p < .05), and no correlation was detected with other variables. However, GDS have signitively negative correlations with resting heart rate(r = -0.464), sit & reach(r = -0.525) left(r = -0.491) and right grip strengths(r = -0.635) as well as positive correlation systolic blood pressure(r = 0.482) and TUG (r = 0.479), respectively(p < .05). In conclusion, long-term aerobic exercise of elderly women over 70 older had positive effects on variables related to metabolic disease (visceral fat and resting heart rate), silver fitness variables related to falls, and depression level in the elderly.

• Journal of the Korean Society of International Agriculture
• /
• v.23 no.5
• /
• pp.537-545
• /
• 2011

This study was conducted to obtain basic information on physiological and proteomic responses of barley seedlings to salt stress. Shoot dry weight decreased significantly as the level of soil salinity increased. Salt stress-induced decrease of relative shoot dry weight was lower in cv. "Sanglok" than in cv. "Sunwoo". Under the salt stress, SPAD value decreased, and the value was higher in cv. "Sanglok" than in cv. "Sunwoo". Sodium ion content in the leaves increased as NaCl concentration increased, and the content was higher in cv. "Sunwoo" than in cv. "Sanglok". The K⁺/Na⁺ ratio was higher in cv. "Sanglok" than in cv. "Sunwoo". Salt stress-induced alterations in protein expression of the leaves were detected by two dimensional electrophoresis, and 47 protein spots showing altered expression were selected. Among the selected protein spots, 17 protein spots were up-regulated and 28 spots down-regulated in cv. "Sanglok". In cv. "Sunwoo", 14 protein spots were up-regulated and 27 spots down-regulated. Out of 47 deferentially expressed protein spots, 18 protein spots were identified using mass spectrometry and NCBI protein database. Among the identified proteins, ten proteins are known to be involved in various stress responses, but the others are not directly involved in stress responses.

• Journal of Korea Water Resources Association
• /
• v.56 no.8
• /
• pp.497-508
• /
• 2023

Agricultural reservoirs are crucial structures for water resources monitoring especially in Korea where the resources are seasonally unevenly distributed. Optical and Synthetic Aperture Radar (SAR) satellites, being utilized as tools for monitoring the reservoirs, have unique limitations in that optical sensors are sensitive to weather conditions and SAR sensors are sensitive to noises and multiple scattering over dense vegetations. In this study, we tried to improve water body detection accuracy through optical-SAR data fusion, and quantitatively analyze the complementary effects. We first detected water bodies at Edong, Cheontae reservoir using the Compact Advanced Satellite 500(CAS500), Kompsat-3/3A, and Sentinel-2 derived Normalized Difference Water Index (NDWI), and SAR backscattering coefficient from Sentinel-1 by K-means clustering technique. After that, the improvements in accuracies were analyzed by applying K-means clustering to the 2-D grid space consists of NDWI and SAR. Kompsat-3/3A was found to have the best accuracy (0.98 at both reservoirs), followed by Sentinel-2(0.83 at Edong, 0.97 at Cheontae), Sentinel-1(both 0.93), and CAS500(0.69, 0.78). By applying K-means clustering to the 2-D space at Cheontae reservoir, accuracy of CAS500 was improved around 22%(resulting accuracy: 0.95) with improve in precision (85%) and degradation in recall (14%). Precision of Kompsat-3A (Sentinel-2) was improved 3%(5%), and recall was degraded 4%(7%). More precise water resources monitoring is expected to be possible with developments of high-resolution SAR satellites including CAS500-5, developments of image fusion and water body detection techniques.

• Journal of Food Hygiene and Safety
• /
• v.38 no.6
• /
• pp.457-463
• /
• 2023

To investigate residual pesticide levels in agricultural products contained in Meal-kits, 27 Meal-kit products were collected from marts, Meal-kit shops, and online stores in Incheon City, South Korea. Seventy-six vegetable and thirty-seven mushroom products were analyzed for residual levels of 339 pesticides. Residual pesticides were detected in 23 out of 76 vegetables and were not present in the 37 mushroom products. The residual pesticide detection rate was 20.4% (23/113 cases). The pesticides famoxadone 0.034 mg/kg (standard: 0.01 mg/kg or less, PLS) and fenpyroximate 0.302 mg/kg (standard: 0.01 mg/kg or less, PLS) exceeded their maximum residue levels (MRL). This survey revealed that various types of pesticides remain in agricultural products in Meal-kits. Due to the nature of Meal-kit products, there is no separate standard for residual pesticides in agricultural products. Therefore, continuous monitoring of residual pesticides is necessary.

• Korean Journal of Plant Resources
• /
• v.36 no.5
• /
• pp.496-507
• /
• 2023

The in vitro organogenesis is one of important issues in plant embryology, and somaclonal variations are existing in calli and/or regenerants induced from a process of the organogenesis with in vitro circumstances. In this study, expressions of organogenesis-related genes were evaluated and genetic stability of regenerants derived from the process of in vitro organogenesis were measured using ISSR markers in Imperata cylindrica 'Rubra', Poaceae. The expressions of organogenesis-related genes were detected all of regenerants at the process of the organogenesis. All ISSR markers produced with an average of 71 bands per in vitro-cultured regenerants, and the scorable bands were varied from two to eight with an average of 5.14 bands per a primer. The polymorphism rates of the in vitro regenerants were higher than that of mother plants (1.4%), showing 4.1% (pot-cultured regenerants), 4.3% (field-cultured regenerants), 4.2% (in vitro-cultured regenerants), 5.6% (calli with green shoots) and 1.4% (calli), respectively. The genetic similarity matrix (GSM) among all accessions ranged from 0.747 to 1.0 with a mean of 0.868. GSM of the regenerants showed differences (from 0.972 to 1.00) compared with that of mother plants (0.991). According to the clustering analysis, two independent groups were divided into; the one is mother plants and regenerants cultured at room and open field, the other is regenerants cultured in vitro. The results give a new insight for understanding the dynamics of organogenesis in monocot plant.

• Korean Journal of Remote Sensing
• /
• v.39 no.5_4
• /
• pp.1173-1183
• /
• 2023

Over the past 20 years, Korea's overall import and export cargo volume has increased at an average annual rate of approximately 5.3%. About 99% of the cargo is still being transported by sea. Due to recent increases in maritime cargo volume, congestion in maritime logistics has become challenging due to factors such as the COVID-19 pandemic and conflicts. Continuous monitoring of ports has become crucial. Various ground observation systems and Automatic Identification System (AIS) data have been utilized for monitoring ports and conducting numerous preliminary studies for the efficient operation of container terminals and cargo volume prediction. However, small and developing countries' ports face difficulties in monitoring due to environmental issues and aging infrastructure compared to large ports. Recently, with the increasing utility of artificial satellites, preliminary studies have been conducted using satellite imagery for continuous maritime cargo data collection and establishing ocean monitoring systems in vast and hard-to-reach areas. This study aims to visually detect ships docked at berths in the Busan New Port using high-resolution satellite imagery and quantitatively evaluate berth utilization rates. By utilizing high-resolution satellite imagery from Compact Advanced Satellite 500-1 (CAS500-1), Korea Multi-Purpose satellite-3 (KOMPSAT-3), PlanetScope, and Sentinel-2A, ships docked within the port berths were visually detected. The berth utilization rate was calculated using the total number of ships that could be docked at the berths. The results showed variations in berth utilization rates on June 2, 2022, with values of 0.67, 0.7, and 0.59, indicating fluctuations based on the time of satellite image capture. On June 3, 2022, the value remained at 0.7, signifying a consistent berth utilization rate despite changes in ship types. A higher berth utilization rate indicates active operations at the berth. This information can assist in basic planning for new ship operation schedules, as congested berths can lead to longer waiting times for ships in anchorages, potentially resulting in increased freight rates. The duration of operations at berths can vary from several hours to several days. The results of calculating changes in ships at berths based on differences in satellite image capture times, even with a time difference of 4 minutes and 49 seconds, demonstrated variations in ship presence. With short observation intervals and the utilization of high-resolution satellite imagery, continuous monitoring within ports can be achieved. Additionally, utilizing satellite imagery to monitor changes in ships at berths in minute increments could prove useful for small and developing country ports where harbor management is not well-established, offering valuable insights and solutions.

• Journal of Korea Water Resources Association
• /
• v.56 no.10
• /
• pp.631-639
• /
• 2023

Flash drought (FD), characterized by the rapid onset and intensification, can significantly impact ecosystems and induce immediate water stress. A more comprehensive understanding of the causes and characteristics of FD events is required to enhance drought monitoring. Therefore, we investigated the FD events took place over the Korean peninsula using Global Land Data Assimilation System (GLDAS) data from 2012 to 2022. We first detected FD events using the stress-based method (Standardized Evaporative Stress Ratio, SESR), and analyzed the frequency and duration of FDs. The FD events were classified into three cases based on the variations in Actual Evapotranspiration (AET) and potential Evapotranspiration (PET), and spatially analyzed. Results revealed that there are regional disparities in frequency and duration of FDs, with a mean frequency of 6.4 and duration of 31 days. When classified into Case 1 (normal condition), Case 2 (AET-driven), and Case 3 (PET-driven), we found that Case 2 FDs emerged approximately 1.5 times more frequently than those driven by PET (Case 3) across the Korean peninsula. Case 2 FDs were found to be induced under water-limited conditions, and led both AET and PET to be decreased. Conversely, Case 3 FDs occurred under energy-limited conditions, with increase in both. Case 2 FDs predominantly affected the northwestern and central-southern agricultural regions, while Case 3 occurred in the eastern region, characterized by forested land cover. These findings offers insights into our understanding of FDs over the Korean peninsula, considering climate factors, land cover, and water availability.

• Journal of Life Science
• /
• v.33 no.11
• /
• pp.887-896
• /
• 2023

The inflammatory response have been considered as one of important targets for cancer treatment because they play a key role during all steps of tumor development including initiation, promotion, malignant conversion and progression. To investigate the anti-inflammatory response during anti-tumor activity of an aqueous extracts of Ecklonia cava (AEC), alterations on the distribution of mast cells and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), nuclear factor (NF)-κB, inflammasome compositional protein and inflammatory cytokines were examined in CT26 colon tumor-bearing BALB/cKorl syngeneic mice after administrating AEC for five weeks. After treatment of AEC, total weight of tumor and necrotic region of tumor section were significantly decreased compared to vehicle treated group. The number of infiltered mast cells was higher in AEC treated group than vehicle treated group, while the expression levels of COX-2 and iNOS were decreased in AEC treated group. Also, similar decrease pattern were detected in the expression levels of NF-κB, NLR family pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and caspase-1 (Cas-1) after AEC treatment although the decrease rate was varied. Furthermore, the mRNA expressions of three inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α) and interleukin-6 (IL-6) were remarkably decreased in AEC treated group compared to vehicle treated group. These results suggest that inhibition of inflammatory response may be tightly associated with anti-tumor activity of AEC in CT26 colon tumor-bearing BALB/cKorl syngeneic mice.