• 한국재료학회지
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• 제20권2호
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• pp.82-89
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• 2010

Benzotriazole (B.T.A) which has been mainly used for the stabilization processing method of excavated copper and bronze artifacts is vaporized within 2~3 years after the usage because it is unstable at the acid conditions and cannot protect the surface of artifacts. In this study, NaOH method which has been used for the steel artifacts was applied as a stabilization process for the method of copper and bronze artifacts to gush chlorine ion out. For the reproduction of excavated samples, copper and bronze plates were dipped in 0.1M HCl for 26 hrs to form CuCl, rusted at $70^{\circ}C$ with RH 75% for the formation of corrosion products, and desalted in 0.1 M NaOH solution. The concentration of chlorine ion was measured by using ionchromatography. During the desalting process, a large quantity of chlorine ions was gushed out in early period and corrosion products were not additionally generated through the re-corrosion experiment. This NaOH desalting process was found to be a method of stabilization process for copper and bronze artifacts from the formation of Tenorite (CuO) during desalting as a protection layer for corrosion.

• 한국식품영양과학회지
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• 제22권3호
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• pp.317-322
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• 1993

배추의 염절임(5$0^{\circ}C$, 25% 소금용액)및 탈염(5$0^{\circ}C$, 증류수)과정중 침지시간에 따른 물질이동을 수분흡수 또는 유출, 소금유출 또는 흡수 및 환원당유출로써 조사하였다. 염절임 과정중 수분유출과 소금흡수는 초기 6시간 침지과정에서 빠르게 이루어진 후 증가현상이 둔화되었다. 24시간 염절임 과정 후 초기 100g 배추무게당 수분유출과 소금흡수는 각각 33.35g과 6.26g이었다. 염절임과정중 수분함량은 94.29%에서 83.11%로 줄어들었다. 환원당농도 역시 29.2mg/$m\ell$에서 6.5mg/$m\ell$로 낮아졌으며, 침지시간(hr) 의 평방근을 이용한 직선식은 Y=30.1841-5.0269√t이었다. 24시간 염절임 한후 탈염과정에서의 수분흡수와 소금유출은 초기 4시간 침지과정에서 빠르게 이루어진 후 직선적으로 계속적인 증가현상을 나타내었다. 12시간 탈염과정 후 초기 100g배추 무게당 수분흡수는 20.82g이 었으며, 소금유출은 9.14g이었다. 탈염과정에서의 소금유출이 염절임과정의 소금흡수보다 높은 이유는 염절임 및 탈염과정에서 배추의 고형분 유출에 기인된 것이었다. 12시간의 탈염공정으로 김치 제조시 배추의 적정 소금농도인 3%로 낮출수 있었다. 이때의 환원당농도는 1.6mg/$m\ell$로 낮아졌으며, 침지시간의 평방근을 이용한 직선식은 Y=6.7854-1.5992√t로써 염절임 공정의 기울기값 (-5.0269)과 비교할때 탈염공정에서의 환원당 겉보기 유출속도는 낮았다.

• Mass Spectrometry Letters
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• 제4권2호
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• pp.25-29
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• 2013

Proteomics for biomarker validation needs high throughput instrumentation to analyze huge set of clinical samples for quantitative and reproducible analysis at a minimum time without manual experimental errors. Sample preparation, a vital step in proteomics plays a major role in identification and quantification of proteins from biological samples. Tryptic digestion a major check point in sample preparation for mass spectrometry based proteomics needs to be more accurate with rapid processing time. The present study focuses on establishing a high throughput automated online system for proteolytic digestion and desalting of proteins from biological samples quantitatively and qualitatively in a reproducible manner. The present study compares online protein digestion and desalting of BSA with conventional off-line (in-solution) method and validated for real time sample for reproducibility. Proteins were identified using SEQUEST data base search engine and the data were quantified using IDEALQ software. The present study shows that the online system capable of handling high throughput samples in 96 well formats carries out protein digestion and peptide desalting efficiently in a reproducible and quantitative manner. Label free quantification showed clear increase of peptide quantities with increase in concentration with much linearity compared to off line method. Hence we would like to suggest that inclusion of this online system in proteomic pipeline will be effective in quantification of proteins in comparative proteomics were the quantification is really very crucial.

• 멤브레인
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• 제16권3호
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• pp.213-220
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• 2006

본 연구에서는 정밀여과 및 나노여과 분리막 시스템을 활용하여 염료 제조 시 필요한 탈염/정제/농축처리 기술을 개발하고 그에 따른 염료의 품질과 품질의 안정성을 향상시키며, 기존공정의 단수를 축소하여 생산성 향상, 인력저감 및 원재료의 비용을 감소하고자 하였다. 염료의 탈염/정제/농축처리시 염료의 다양한 농도에 적합한 분리막의 제조 및 가압식 막 element를 제작하고, 가압식 분리막 시스템에 맞는 막 모듈의 설계와 pilot-scale 시스템을 통해 염료의 처리수질 분석 및 운전인자를 도출하였다.

• 한국식품저장유통학회지
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• 제19권6호
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• pp.893-900
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• 2012

본 연구에서는 시판 멸치액젓에 주정을 일정한 비율로 첨가하여 소금의 용해도를 낮춤으로서 소금을 석출시키는 탈염방법을 활용하여 저염 멸치액젓의 제조공정을 확립하고자 하였다. 또한 시판 멸치액젓과 주정을 활용하여 탈염처리 한 저염 멸치액젓의 품질 특성을 비교하기 위해 일반성분, 총질소, 아미노태 질소, 대장균 및 아미노산 함량을 비교분석 하였다. 시판 멸치액젓에 대해 주정의 첨가량이 증가할수록 멸치액젓의 염도는 낮아지는 경향을 보였다. 즉, 멸치액젓 100 mL에 대해 주정을 각각 100% 및 300%(v/v)를 첨가한 경우에 멸치액젓의 염도는 14.3% 및 10.3%를 나타내었다. 이와 같은 현상은 염도 23%인 식염수 용액의 실험에서도 같은 경향을 보였다. 탈염처리 한 멸치 액젓의 총질소 함량은 1.20%이었고, 아미노태 질소 함량은 784.03 mg/100 g으로, 시판 멸치액젓보다 높은 함량을 나타내었다. 시판 멸치액젓과 탈염처리 한 저염 멸치액젓의 주요 총아미노산은 glutamic acid, alanine, lysine 및 leucine이었고, 필수아미노산의 함량은 탈염처리 한 멸치액젓에서 높게 나타났다. 또한, 시판 멸치액젓과 탈염처리 한 저염 멸치액젓의 주요 유리아미노산은 공통적으로 glutamic acid, leucine, alanine, valine, lysine 및 isoleucine 이었다. 이상의 결과에서 주정을 이용하여 소금을 원하는 농도만큼 특별한 장비없이 효과적으로 제거할 수 있는 멸치액젓의 탈염공정을 확립할 수 있었다. 또한 주정을 사용하여 멸치액젓을 탈염하는 방법은 총질소, 아미노태 질소 함량 및 아미노산 함량에 긍정적인 영향을 주어 맛을 향상시켜 줄 수 있을 것으로 기대되며, 본 연구의 결과로 저염 멸치액젓 상품화 연구의 기초자료로 활용 및 인류 보건향상에 크게 기여할 것으로 전망된다.

• 한국수산과학회지
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• 제53권2호
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• pp.174-180
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• 2020

The mouse bioassay has been used widely for the monitoring of paralytic shellfish toxins (PSTs) in many countries. However, this method shows low sensitivity and high limit of detection (LOD), as well as it cannot confirm toxic profiles. Recently, LC-MS/MS method was studied for the quantitative of PSTs, however, the method has any problems with unstable retention times by ionization suppression caused by high salt concentration in shellfish extracts. To establish an alternative method for PSTs analysis, we tried to original LC-MS/MS methods adding desalting operation using amorphous graphitized polymer carbon solid-phase extraction cartridges. The method validation was conducted to determine linearity, limit of detection, limit of quantification (LOQ), accuracy, and precision in quantifying PSTs. The correlation coefficients for all tested PSTs maintained over 0.999. The LODs and LOQs for all PSTs were about 0.19-1.05 ㎍/kg and 0.58-3.18 ㎍/kg, respectively. The accuracies for PSTs were 95.4-107.7% for saxitoxin group, 97.1-100.9% for gonyautoxin group, 99.0-100.8% for N-sulfocarbamoyl toxin group, and 96.8-104.6% for decarbamoyl toxin group. These results indicate that the modified LC-MS/MS method was appropriate for analyzing the PSTs in shellfish and tunicates.

• Ocean and Polar Research
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• 제28권2호
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• pp.187-199
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• 2006

The Kuroko-type seafloor massive sulfide deposits found in the western Pacific have been considered to have potentials for economic recovery of Au, Ag, Cu, Zn, and Pb. In this study, a preliminary model was developed for the technical and economic evaluation of them. The FRSC site on Lau Basin in the Tonga EEZ was selected as a target. In this study, no construction In for the metallurgical processing subsystem was accounted for. Instead, it was assumed to sell the Cu, Zn, and Pb concentrates to the existing sulfide customer smelter. The low total investment costs for the development make the venture very attractive. However, the result of the economic feasibility evaluation is still less attractive with the mean metal yield of the Kuroko on land. It is considered that commercial mining may be plausible if the richer metal yields are applied to the development. Quantitative information for metal yield is necessary for a more accurate evaluation. However, the important resource potential information regarding the amount of ore body, the inside structure, and the metal yields have not yet been clarified sufficiently. h addition, the flotation of ore body using seawater has not been tested yet. It is necessary to solve these problems through the experimental R&D and a survey.

• 생약학회지
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• 제20권1호
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• pp.25-31
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• 1989

In order to establish the standard method for the preparation of processed Aconiti Tuber, Aconiti Tubers were processed under various conditions and the amount and the composition of alkaloids were determined by HPLC. The ratio of sum of benzoylhypaconine and benzoylmesaconine over the sum of acinitine, mesaconitine, benzoylmesaconine and benzoylhypaconine was used as a detoxification index ((BM+BH)${\times}$100/MA+AC+BM+BH). The adequate value of index was obtained from Japanese 'ka-gong bu-ja' which has been used in Japan. The processing procedure was largely devided into two categories. First is heat treating at $120^{\circ}$ and 1. 2 lbs for 60 min. Second is treatment with various kinds of alkaline solutions followed by heat treatment at $120^{\circ}$ and 1. 2 lbs for 60 min. Among the source of processed Aconiti Tubers, dried bu-ja and yom bu-ja, dried bu-ja was more adequate than yom bu-ja because yom bu-ja has the lower value of index than dried bu-ja and lost active components through the desalting periods. Dried bu-ja whish was treated with alkaline solutions followed by heat treatment has the detoxification index, 50% and dried bu-ja which was treated only with hear has 71. 8%. Compared to the value of index of Japanese 'ka-gong bu-ja', 72%, the dried bu-ja treated with heat at $120^{\circ}$ and 1, 2 lbs for 60min was the most adequate. The $LD_{50}$ value of the processed bu-ja was higher than 15 g crude drugs/kg, p.o. in mice.

• 한국식품과학회지
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• 제25권2호
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• pp.118-122
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• 1993

염장저장한 고냉지 배추의 1주간 저장후의 탈수율은 39.21%, 2주 후에는 44.62%로서 저장후 2주까지 탈수가 계속되었고, 염도는 3주차에 가장 높아서 5% 처리구는 3.46%, 10% 처리구는 7.27%, 15% 처리구는 9.04%, 20% 처리구는 10.59%, 30% 처리구는 14.82%를 나타냈으며, 이들 배추를 4시간 동안 3배의 물에 담그어 탈염한 결과 염도는 각각 2.71%, 4.67%, 5.38%, 6.84%, 9.43%로 낮아졌고, 저장기간 중 pH는 소금농도가 낮을수록 빨리 저하되어 5% 처리구는 2주후 3.75, 10% 처리구와 15%처리구는 4주후 각각 3.86, 3.55로 저하되었으며, 5% 처리구에서 1주차에 4.38, 2주차에 3.75로 낮아져 pH저하속도가 빨랐으며 10% 처리구는 1주차에 4.93으로 빨리 떨어지고 그후 직선적으로 떨어져 4주후에는 3.86으로 되고 15% 처리구에서는 4주차까지 직선적으로 떨어져서 3.55가 되었다. 이들 저장배추의 염도, pH, 맛, 향, 색, 조직감 등을 종합적으로 판단한 결과 소금농도 10% 이하로 저장된 배추는 저장 4주차까지 김치재료로 사용이 가능한 것으로 판단되었다.