• Title/Summary/Keyword: density of media

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Tight junctional inhibition of entry of Toxoplasma gondii into MDCK cells (MDCK세포의 tight junction 형성이 Toxoplusmu gondii의 숙주세포 침투에 미치는 효과)

  • 남호우;윤지혜
    • Parasites, Hosts and Diseases
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    • v.28 no.4
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    • pp.197-206
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    • 1990
  • Various conditions of cultures were performed to investigate the role of tight junctions formed between adjacent MDCK cells on the entry of Toxoplasma. When MDCK cells were cocultured with excess number of Toxoplasma at the seeding density of 1×105, 3×105, and 5×105 cells/ml for 4 days, the number of intracellular parasites decreased rapidly as the host cells reached saturation density, i.e., the formation of tight junctions. When the concentration of calcium in the media (1.8 mM in general) was shifted to $5{\mu}M$ that resulted in the elimination of tight junction, the penetration of Toxoplasma increased about 2-fold(p<0.05) in the saturated culture, while that of non-saturated culture decreased by half. Trypsin-EDTA which was treated to conquer the tight junctions of saturated culture favored the entry of Toxoplasma about 2.5-fold(P<0.05) compared to the non-treated, while that of non- saturated culture decreased to about one fifth. It was suggested that the tight junctions of epithelial cells play a role as a barrier for the entry of Toxoplasma and Toxoplasma penetrate into hoot cells through membrane structure-specific, i.e., certain kind of receptors present on the basolateral rather than apical surface of MDCK cells.

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Influence of Paper Mill Sludge and Briquet Ash on the Growth of Zoysiagrass (제지(製紙)슬러지와 연탄재가 들잔디의 생육(生育)에 미치는 영향(影響))

  • Ku, Ja Hyeong;Kim, Tae Il;Ahn, Joo Won;Kim, Moon Kyu
    • Korean Journal of Agricultural Science
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    • v.19 no.2
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    • pp.153-160
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    • 1992
  • To investigate the potential of paper mill sludge and briquet ash as cultural media in turfgrass, zoysiagrass was grown in the mixtures prepared with paper mill sludge and briquet ash. The mixtures were consisted of sludge and briquet ash in the ratio by volume 3:1, 2:1, 1:1, 1:2, and 1:3 in the order. To compare the growth responses, some plants were grown in the medium containing sand(3), field soil(1) and peatmoss (1) by volume anti regarded as control. 1. Activated sludge mixed with sand increased plant height, fresh weight, and dry weight more than 1.5-2.0 times compared to those of the control. The highest plant growth was shown in mixtures containing 67% activated sludge. 2. Plant density per $100cm^2$ and chlorophyll content were higher in all mixtures containing activated sludge than control. 3. The growth of zoysiagrass was reduced along with the increase of non-activated sludge ratio, but no difference was found in chlorophyll content. 4. Plant height, fresh weight and dry weight were greater in activated sludge combined with sand compared to the briquet ash mixtures, but the difference of shoot density between two mixtures was not shown. 5. Even though non-activated sludge appeared not to be appropriate to the growth of seedlings, the number of tillers of plant propagated with rhizome was more increased compared to control when briquet ash content was less than 75%.

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Effect of Split Nitrogen Application Times on Turf Vegetation of Creeping Bentgrass (질소 분시횟수가 Creeping Bentgrass 잔디초지의 식생에 미치는 영향)

  • Park, Sung-Jun;Cho, Nam-Ki;Kang, Young-Kil;Song, Chang-Khil;Cho, Young-Il
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.2
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    • pp.119-124
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    • 2005
  • This study was conducted from March 21 to July 8 in 2004 at JeJu Island to investigate the influences of split nitrogen application times on creeping bentgrass vegetation. Nitrogen rate was 20 kg/10a and it was applied from once to 5 times. The result obtained were summarized as follows; plant height was getting longer as nitrogen was split applied from once to 5 times. So it was longest at 5 times split nitrogen application, but it was no significance from 4 to 5 times. Root length, Minolta SPAD-502 chlorophyll meter reading value, leave and root weight n[e directly proportional plant height response. Degree of land cover Iud density of creeping bentgrass increased as nitrogen was split applied from once to 5 times. But degree of land cover md density of reed decreased. The number of reed species on decreased as nitrogen was split applied from once to 5 times. Then ranking of the dominant weeds were Portulaca oleracea Polygonum hydropiper and Stellaria media (at once split time nitrogen application), Portulaca oleracea, Polygonum hydropiper and Digitaria adscendens (at 2 split times nitrogen application), Digitaria adscendens and Portulaca oleracea Polygonum hydropiper, (at 3 split times nitrogen application), Portulaca oleracea, Poa annua and Polygonum hydropiper (at 4 md 5 split times nitrogen application). These results indicate that the optimum frequency of split N applications is four times for growth of creeping bentgrass in volcanic ash soils of Jeju island.

Management of Diabetic Mellitus in Low-income Rural Patients (일개 농촌지역 저소득층 당뇨병환자의 관리 상태)

  • Kim, Hye-Yeon;Yun, Woo-Jun;Shin, Min-Ho;Kweon, Sun-Seong;Ahn, Hye-Ran;Choi, Seong-Woo;Lee, Young-Hoon;Cho, Dong-Hyeok;Rhee, Jung-Ae
    • Journal of Preventive Medicine and Public Health
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    • v.42 no.5
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    • pp.315-322
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    • 2009
  • Objectives : Knowledge about the management status of diabetic melitus (DM) is essential to improve diabetic management. Moreover, low income is associated with poor adherence to treatment and increased mortality. This study was performed to evaluate the management status of DM in low-income patients in a rural area. Methods : We enrolled 370 patients with type 2 DM living in Gokseong county, JeollaNamdo. A well-trained examiner measured the height, weight, waist circumference, blood pressure, total cholesterol, triglyceride, high density lipoprotein cholesterol, fasting blood sugar and glycosylated hemoglobin (HbA1c) levels. Carotid ultrasonography was used to measure carotid artery carotid artery intima media thickness (IMT) and plaque. anklebrachial index (ABI) was used to evaluate peripheral artery disease. A fundoscopic examination was performed to evaluate diabetic retinopathy. A history of diabetes complications and health-related questionnaires were also completed. Results : The age of diabetic subjects was 68.7$\pm$8.7 years and the duration of diabetes was 8.9$\pm$8.2 years. Most (63.5%) had hypertension, and 45.7% had triglycerides below 150 mg/dl, 38.1% had low density lipoprotein cholesterol (LDL) cholesterol below 100 mg/dl, 48.7% had urine albumin to creatinine ratio (UACR) below 30 mg/g. Less than half (45.9%) achieved the goal of HbA1c less than 7% suggested by the American Diabetes Association (ADA). 10.6% had peripheral vascular disease, 11.9% had retinopathy, and 60.8% had chronic kidney disease. Conclusions : DM management in low income patients is very poor and requires further work to improve.

Development on Artificial Cultivation method of Hatakeshimeji (Lyophyllum decastes) using fermented sawdust substrate (잿빛만가닥버섯(Lyophyllum decastes)의 발효톱밥에 의한 인공재배 특성에 관한 연구)

  • Woo, Sung-Mi;Park, Yong-Hwan;Yoo, Young-Bok;Shin, Pyung-Gyun;Jang, Kab-Yeul;Lee, Kang-Hyo;Sung, Jae-Mo
    • Journal of Mushroom
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    • v.7 no.4
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    • pp.156-162
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    • 2009
  • These experiments were carried out to determine the optimal culture conditions for nine strains of collected Hatakeshimeji, Lyophyllum decastes (Fr.:Fr.) Sing. SPA 202 and SPA 205 strains were selected because mycelium grew fast and showed fine density. All strains showed fast mycelial growth and mycelial density on BC(Burke compost) media for 20 days of incubation. The optimal sawdust species for the mycelial growth were the fermented sawdusts of Quercus aliena and Populus deltoides. Spawn running period on the fermented sawdust substrate required 50 days at 20 to $25^{\circ}C$ and additional 7 days after soil casing. Cultivation period and temperature forprimordia formation and fruitbody development appeared from 10 to 11 days and from 7 to 8 days at 17 to $18^{\circ}C$ respectively. The length of pilei and stipes of SPA 202 harvested in optimal stage showed 60mm and 67mm, respectively. Yield of SPA 202 strain grown on fermented sawdust substrate was 130g per 1,100ml in bottle cultivation. The length of pilei and stipes of SPA 205harvested in optimal stage showed 51mm and 81mm, respectively. Yield of SPA 205 strain grown on fermented sawdust substrate was 129g per 1,100 ml in bottle cultivation. SPA 202 strain and SPA 205 strain in artificial bottle cultivation of Lyophyllum decastes used in fermented sawdust substrate were selected as themost appropriate strain in yield.

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Layer-separable PES Packetization and Processing Scheme for SVC-based Satellite Broadcasting Service (SVC 기반의 위성방송 서비스를 위한 계층 분리형 PES 패킷화 및 처리 기법)

  • Chi, Won-Sup;Seo, Kwang-Deok;Kim, Jin-Soo;Lee, In-Ki;Chang, Dae-Ig
    • Journal of Broadcast Engineering
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    • v.14 no.5
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    • pp.561-572
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    • 2009
  • In this paper, we propose an efficient layer-separable PES packetization and processing scheme for DVB-S2 satellite broadcasting service based on SVC video. Unlike the conventional single layer-based video coding such as MPEG-2, MPEG-4 and H.264, SVC can combine numerous number of video layers, which are aggregated to a single bitstream. Therefore, it is necessary to devise a new PES packetization scheme that can efficiently separate multiple video layers of SVC. In order to combine the layered characteristics of the SVC video and the robust channel coding capability of LDPC (Low Density Parity Check) of DVB-S2 for unequal error protection, we propose an efficient PES packetization in the transmitter side and PES packet processing scheme in the receiver side of DVB-S2. We prove the effectiveness of the proposed scheme in terms of processing speed and time delay required for processing of the separated layers of SVC video in the satellite broadcasting service.

A Study on Efficacy Of Small Examination According to The W/V% Barium Suspension Mixed With Sodium-Carboxy Methyl Cellulose ($BaSO_4$ Mixed CMC-Na 조영제의 특성과 W/V%농도 변화에 따른 경구 소장조영 검사법의 유용성에 관한 연구)

  • Uhm, Joon-Yong;Lee, Eun-Joo;Lee, Yang-Sub;Lee, Won-Hong;Cho, Cheong-Chan;Ryu, Myung-Seon
    • Journal of radiological science and technology
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    • v.26 no.1
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    • pp.39-44
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    • 2003
  • The aim of this study is to evaluate a efficient w/v% barium suspension and w/v% sodium carboxy methyl cellulose (SCMC) for small bowel examination. Between november 2001 and june 2002, 370 patients were examined small bowel examination with oral administration of barium suspension mixed with SCMC. we classified into six groups including A(fine type 40% $BaSO_4$ mixed 0.5% SCMC and 600 ml 0.5% SCMC administration), B(fine type 30% $BaSO_4$ mixed 0.5% SCMC), C(fine type 20% $BaSO_4$ mixed 0.5% SCMC), D(fine type 25% $BaSO_4$ mixed 0.5% SCMC), E(D mixed coarse type 25% $BaSO_4$ mixed 0.5% SCMC), F(D mixed coarse type 25% $BaSO_4$ mixed 0.75% SCMC). We measured transparency rate of contrast media transit time, administration dose, viscosity and particle size. The transparency rate was higher then 80 kV and 90 kV in 100 kV in the same 20 mAs condition. Transit time was the fastest in A group(mean trans time 56 minutes) and the slowest in E group(mean 100 minutes), Administration dose was the smallest in A group(mean dose 541 ml and the most in E group(mean 1,100 ml), viscosity was the lowest in E(125.1 mpa/s) and the highest in A(375.5 mpa/s), and particle size was $1.0\;{\mu}m$ in A, B, C, and D group, $0.6\;{\mu}m,\;1.0\;{\mu}m,\;10.0\;{\mu}m$ in E, F group. In conclusion, we propose that the efficient condition for small examination is high voltage technique, high density $BaSO_4$, and 0.625% w/v SCMC.

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Control of the Root-Knot Nematode (Meloidogyne spp.) on Cucumber by a Liquid Bio-Formulation Containing Chitinolytic Bacteria, Chitin and Their Products (키틴분해세균, 키틴 및 그들의 산물이 함유된 미생물제에 의한 오이의 뿌리혹선충(Meloidogyne spp.) 방제)

  • Ha, Woo Jong;Kim, Young Cheol;Jung, Hyuncha;Park, Seur Kee
    • Research in Plant Disease
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    • v.20 no.2
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    • pp.112-118
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    • 2014
  • A liquid bio-formulation containing chitinolytic bacteria, chitin and their products was assessed for its potential biological control against root-knot nematodes on cucumber. The bio-formulation was prepared by cultures of three chitinolytic bacteria, Chromobacterium sp. strain C-61, Lysobacter engymogenes and Serratia plymuthica in minimal medium supplemented with chitin. Under pot conditions, the bio-formulation showed better growth of cucumber plants, and less root galls and population density of Meloidogyne spp. than control media without the bio-formulation. In a greenhouse, 75-fold diluted bio-formulations were treated instead of water around cucumber plants through hoses for drip irrigation six times at 5-day intervals from the transplanting date. After 30 and 60 days, the treatment provided about 7% and 10% enhancement in the plant height and about 78% and 69% reduction in population density of Meloidogyne spp. in the rhizosphere, respectively. In addition, the experiments showed that the control effects occurred only in the soils contacted with the bio-formulation. Undiluted bio-formulations were drenched three times at 10-day intervals around cucumber plants severely infested with Meloidogyne spp. The treatment showed about 37% plant enhancement without dead plants compared with 37% death in the untreated control, and about 82% nematode reduction. These results suggest that the bio-formulation can be practically used to control the root-knot nematode on cucumber.

Magnetic Properties of Fe4N Nanoparticles and Magnetic Fe17Sm2Nx Powders (Fe4N 나노분말과 Fe17Sm2Nx 자성분말의 자기적 특성)

  • Oh, Young-Woo;Lee, Jung-Goo;Park, Sang-Jun
    • Journal of the Korean Magnetics Society
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    • v.22 no.3
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    • pp.79-84
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    • 2012
  • Nano-magnetic materials such as iron-nitrides have been actively studied as an alternative to the application of high density, high performance needs for next generation information storage and also alternative to the rare earth and neodymium magnet. $Fe_4N$ is the basic materials for magnetic storage media and is one of the important magnetic materials in focus because of its higher magnetic recording density and chemical stability. Single phase ${\gamma}^{\prime}-Fe_4N$ nanoparticles have been prepared by a PAD (Plasma Arc Discharge) method and nitriding in a $NH_3-H_2$ mixed gases at temperature, $400^{\circ}C$ for 4 hrs. Also $Fe_{17}Sm_2N_x$ powders were synthesized by nitriding after reduction/diffusion of $Fe_{17}Sm_2$ to compare the magnetic properties with nano-sized $Fe_4N$ particles. The saturation magnetization of $Fe_4N$ and $Fe_{17}Sm_2N_x$ were 149 and 117 emu/g, respectively, but the coercive force was considerably smaller than that of bulk or acicular $Fe_4N$.

A new approach for overlay text detection from complex video scene (새로운 비디오 자막 영역 검출 기법)

  • Kim, Won-Jun;Kim, Chang-Ick
    • Journal of Broadcast Engineering
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    • v.13 no.4
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    • pp.544-553
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    • 2008
  • With the development of video editing technology, there are growing uses of overlay text inserted into video contents to provide viewers with better visual understanding. Since the content of the scene or the editor's intention can be well represented by using inserted text, it is useful for video information retrieval and indexing. Most of the previous approaches are based on low-level features, such as edge, color, and texture information. However, existing methods experience difficulties in handling texts with various contrasts or inserted in a complex background. In this paper, we propose a novel framework to localize the overlay text in a video scene. Based on our observation that there exist transient colors between inserted text and its adjacent background a transition map is generated. Then candidate regions are extracted by using the transition map and overlay text is finally determined based on the density of state in each candidate. The proposed method is robust to color, size, position, style, and contrast of overlay text. It is also language free. Text region update between frames is also exploited to reduce the processing time. Experiments are performed on diverse videos to confirm the efficiency of the proposed method.