• Molecules and Cells
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• 제40권7호
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• pp.495-502
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• 2017

The $5-HT_6R$ has been considered as an attractive therapeutic target in the brain due to its exclusive expression in the brain. However, the mechanistic linkage between $5-HT_6Rs$ and brain functions remains poorly understood. Here, we examined the effects of $5-HT_6R$-mediated cell morphological changes using immunocytochemistry, Western blot, and live-cell imaging assays. Our results showed that the activation of $5-HT_6Rs$ caused morphological changes and increased cell surface area in HEK293 cells expressing $5-HT_6Rs$. Treatment with 5-HT specifically increased RhoA-GTP activity without affecting other Rho family proteins, such as Rac1 and Cdc42. Furthermore, live-cell imaging in hippocampal neurons revealed that activation of $5-HT_6Rs$ using a selective agonist, ST1936, increased the density and size of dendritic protrusions along with the activation of RhoA-GTP activity and that both effects were blocked by pretreatment with a selective $5-HT_6R$ antagonist, SB258585. Taken together, our results show that $5-HT_6R$ plays an important role in the regulation of cell morphology via a RhoA-dependent pathway in mammalian cell lines and primary neurons.

• 생명과학회지
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• 제19권8호
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• pp.1062-1066
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• 2009

N-Acetylglucosamine kinase (GlcNAc kinase or NAGK; EC 2.7.1.59)는 GlcNAc를 인산화하여 GlcNAc-6-phosphate를 만드는 효소이다. 효소자체에 대한 자세한 연구에도 불구하고 포유류에서NAGK의 표현에 대한 연구는 거의 없다. 배양한 흰쥐의 해마신경세포에서 NAGK은 세포체/가지돌기 영역에서 클러스터(cluster)를 형성한다. 본 연구에서는 가지돌기의 긴 축에서부터 가쪽으로 돌출되는 NAGK 클러스터에 대하여 연구하였다. 배양한 해마신경세포를 NAGK와 다양한 연접표지단백질에 대한 항체로 이중염색한 결과 NAGK 클러스터가 가지돌기의 바닥에는 있었지만 억제성 연접후부위에는 존재하지 않았다. 또한 흰쥐 전뇌(forebrain)의 균질액(homogenate, BH), 연접체(synaptosome, S), 연접후치밀질(postsynaptic density, PSD) 분획을 NAGK 항체로 면역염색한 결과 NAGK는 연접체에는 있었지만 PSD 분획에는 존재하지 않았다. 이러한 결과들은 NAGK가 가지돌기가시(spine)의 바닥쪽으로 돌출됨을 의미한다.

• 한국고분자학회:학술대회논문집
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• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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• pp.69-70
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• 2006

In lithium-ion batteries, separator membrane's, main role is to physically isolate a cathode and an anode while maintaining rapid transport of ionic charge carriers during the passage of electric current. As far as battery safety is concerned, the electrical isolation of electrodes is most crucial since unexpected short-circuits across the membrane induces hot spots where thermal runaway may break out. Internal short-circuits are generally believed to occur by protrusions on the electrode surface either by unavoidable deposits of metallic impurities or by dendritic lithium growth during battery operation. Another cause is shrinkage of the separator membrane when exposed to heat. If separator membrane can be engineered to prevent the internal short-circuit, it will not be difficult to improve lithium-ion batteries' safety. Commonly the separators employed in lithium-ion batteries are made of polyethylene (PE) and/or polypropylene (PP). These materials have terrible limitations in preventing the fore-mentioned internal short-circuit between electrodes due to their poor dimensional stability and mechanical strength. In this study we have developed a novel separator membrane that possesses very high thermal and mechanical stability. The cells employing this separator provided noticeable safety improvement in the various abuse tests.