• 대한수의학회지
• /
• 제45권2호
• /
• pp.207-214
• /
• 2005

The nonstructural protein 4 (NSP4) of rotavirus encoded by gene 10, plays an important role in rotavirus pathogenicity. In this study, NSP4 gene of avian rotavirus (AvRV-1, AvRV-2) was analyzed and expressed using baculovirus expression system. The sequence data indicated that the NSP4 gene of AvRV-1 and AvRV-2 were 727 bases in length, encoded one open reading frame of 169 amino acids beginning at base 41 and terminating at base 550, and had two glycosylation sites. Nucleotide sequences of NSP4 gene of AvRV-1 and AvRV-2 exhibited a high degree of homology ($88.1{\pm}7.6%$) with avian rotaviruses, namely Ty1, Ty3 and PO-13. Phylogenetic analysis showed that AvRV-1 and AvRV-2 belonged to genotype NSP4[E], which is widely found in group A avian rotaviruses. The baculovirus-expressed NSP4 migrated at 20-28 kDa and reacted with NSP4-specific antiserum by FA and Western blot. Furthermore, it was found to be a glycoprotein by using tunicamycin, which is a specific inhibitor of N-linked glycosylation.

• Journal of Microbiology and Biotechnology
• /
• 제22권5호
• /
• pp.642-648
• /
• 2012

We determined the nucleotide sequence of the URA3 gene encoding orotidine-5'-phosphate decarboxylase (OMPDCase) of the erythritol-producing osmotolerant yeast Candida magnoliae by degenerate polymerase chain reaction and genome walking. Sequence analysis revealed the presence of an uninterrupted open-reading frame of 795 bp, encoding a 264 amino acid residue protein with the highest identity to the OMPDCase of the yeast Kluyveromyces marxianus. Phylogenetic analysis of the deduced amino acid sequence revealed that it shared a high degree of identity with other yeast OMPDCase homologs. The cloned URA3 gene successfully complemented the ura3 null mutation in Saccharomyces cerevisiae, revealing that it encodes a functional OMPDCase in C. magnoliae. An enzyme activity assay and reverse transcription polymerase chain reaction indicated that the expression level of the C. magnoliae URA3 gene in S. cerevisiae was not as high as that of the S. cerevisiae URA3 gene. The GenBank accession number for C. magnoliae URA3 is JF521441.

• 한국전기전자재료학회논문지
• /
• 제18권5호
• /
• pp.484-489
• /
• 2005

Because of its effectiveness for the PD(Partial Discharge) pattern recognition, PSA(Pulse Sequence Analysis) has been considered as a new analytic method instead of conventional PRPDA(Phase Resolved Partial Discharge Analysis). However, it is generally thought that PSA has some possibility to misjudge patterns in case of data-missing resulting from poor sensitivity because it analyses the correlation between sequential pulses, which leads to hesitate to apply it to on-site. Therefore, in this paper, the problems of PSA such as data-missing and noise-adding cases were investigated. for the purpose, PD data obtained from various defects including noise-adding data were used and analyzed. As a result, it was shown that both cases could cause fatal errors in recognizing PD patterns. In case of the data missing, the error was dependant on the kinds of defect and the degree of degradation Also, it could be noticed that the error due to adding noises was larger than that due to some data missing.

• Investigative Magnetic Resonance Imaging
• /
• 제13권1호
• /
• pp.9-14
• /
• 2009

T1, T2강조영상, FLAIR (fluid attenuated inversion recovery) 영상기법은 뇌 MRI의 가장 기본적인 영상기법들이다. T1강조영상은 짧은 TR과 짧은 TE를 이용한 스핀에코 기법으로서 조직의 T1이완시간의 차이를 신호 차이로 반영하는 기법이다. 짧은 TR을 사용하면 조직 간에 종축 자기화의 회복 정도가 크게 차이나게 되며 이를 신호에 반영하는 것이다. T2강조영상은 긴 TR과 긴 TE를 이용한 스핀에코 기법으로서 조직의 T2이완시간의 차이를 신호 차이로 반영하는 기법이다. 긴 TE을 사용하면 조직 간에 횡축 자기화의 붕괴가 크게 차이나게 되며 이를 신호에 반영하는 것이다. FLAIR는 180도 반전펄스를 먼저 가하는 반전회복 (inversion recovery) 기법의 일종으로서 뇌척수액의 신호를 억제하기 위하여 2500 msec 정도의 반전시간을 적용한다.

• 방송공학회논문지
• /
• 제26권7호
• /
• pp.838-843
• /
• 2021

This paper presents an algorithm for effective retrieval of score information to an input score image. The originality of the proposed algorithm is that it is designed to be robust to recognition errors by an OMR (Optical Music Recognition), while existing methods such as pitch histogram requires error induced OMR result be corrected before retrieval process. This approach helps people to retrieve score without training on music score for error correction. OMR takes a score image as input, recognizes musical symbols, and produces structural symbolic notation of the score as output, for example, in MusicXML format. Among the musical symbols on a score, it is observed that filled noteheads are rarely detected with errors with its simple black filled round shape for OMR processing. Barlines that separate measures also strong to OMR errors with its long uniform length vertical line characteristic. The proposed algorithm consists of a descriptor for a score and a similarity measure between a query score and a reference score. The descriptor is based on note-count, the number of filled noteheads in a measure. Each part of a score is represented by a sequence of note-count numbers. The descriptor is an n-gram sequence of the note-count sequence. Simulation results show that the proposed algorithm works successfully to a certain degree in score image-based retrieval for an erroneous OMR output.

• 미생물학회지
• /
• 제54권4호
• /
• pp.445-447
• /
• 2018

한국의 전통시장에서 구입한 김치에서 분리된 Leuconostoc lactis CCK940은 sucrose와 maltose를 이용하여 중합도가 4이상인 올리고당을 생산하였다. L. lactis CCK940의 유전체는 1,741,511 bp의 2개 contig로 구성된 염색체로 조합되었으며 G + C의 비율은 43.33%로 나타났다. 염색체 DNA에서 1,698개의 코딩 유전자, 12개의 rRNA, 68개의 tRNA 유전자가 확인되었다. L. lactis CCK940은 올리고당을 생산할 수 있는 sucrose phosphorylase, maltose phosphorylase, ${\beta}$-galactosidase 등의 glucosyltransferase 생합성 유전자들을 지니고 있었다.

• Animal cells and systems
• /
• 제1권3호
• /
• pp.463-466
• /
• 1997

We have recently demonstrated, by protein and cDNA sequence analysis, that prorenin converting enzyme (PRECE) in the mouse submandibular gland is identical to the epidermal growth factor-binding protein (EGF-BP)type B. However, type A and C did not show prorenin converting activity. To demonstrate whether r-NGF is involved in prorenin processing, we have cloned cDNA of r-NGF and examined prorenin converting activity using the CHO cell expression system, Trypsin converted the 33 kDa r-NGF precursor produced in CHO cells to a two-chain form, 9.4 and 16.4 kDa polypeptide chains, which has been known as an active form of r-NGF in mouse SMG (Server and Shooter, 1976). However, the two chain forms of r-NGF did not reveal prorenin-processing activity. Thus, only PRECE is involved in prorenin processing in mouse SMG. This result shows that their substrate specificities appear to be very strict, although some kallikreins share a high degree of amino acid sequence identity.

• 한국측량학회지
• /
• 제27권1호
• /
• pp.723-733
• /
• 2009

This study proposes a sequence of procedures to extract building boundaries and planar patches through segmentation of rasterized lidar data. Although previous approaches to building extraction have been shown satisfactory, there still exist needs to increase the degree of automation. The methodologies proposed in this study are as follows: Firstly, lidar data are rasterized into grid form in order to exploit its rapid access to neighboring elevations and image operations. Secondly, propagation of errors in raw data is taken into account for in assessing the quality of gradients-driven properties and further in choosing suitable parameters. Thirdly, extraction of planar patches is conducted through a sequence of processes: histogram analysis, least squares fitting, and region merging. Experimental results show that the geometric components of building models could be extracted by the proposed approach in a streamlined way.

• 대한산업공학회지
• /
• 제21권1호
• /
• pp.51-65
• /
• 1995

This paper presents an automatic fixture planning system for machining processes of prismatic parts. A rationalized approach to integrate fixture planning with process planning is proposed and representation schemes for workpiece, part design information with features, machine tools, cutting tools and fixtures are developed. The proposed system implements two activities of fixture planning such as machining of reference surfaces and machining of features. For machining of reference surfaces, the machining sequence of reference surfaces is determined by using decision tables, which are drawn from relations of part dimension, degree of surface roughness, fixture type and its capacity, cutting tool's capacity and experienced planners' knowledge. For machining of features, a preferential machining orientation is selected for its feature which can be machined in more than one direction, and features with the same machining orientation are grouped, and the machining sequence of features is determined by interactive mode. A case study is performed to show the performance of the proposed system.

• Journal of Microbiology and Biotechnology
• /
• 제11권6호
• /
• pp.946-950
• /
• 2001

Slow-growing pathogenic mycobacterium species, including Mycobacterium bovis BCG, secrete a large amount of glutamine synthetase into culture media. Extracellular and intracellular glutamine synthetases were purified from M. bovis BCG. While the native molecular weights of both glutamine synthetases were estimated to be 370.2 kDa, those of the subunits were 61.7 kDa, indicating that the native forms were composed of 6 subunits. The enzymes showed a hhigh thermal stability and high degree of sequence similarity with the glutamine synthetase from M. tuberculosis in the N-terminal amino acid sequence. Western blotting analysis indicated that the antibodies prepared against both the extracellular and intracellular enzymes exhibited common antigen determinants.