• 한국연초학회지
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• 제17권2호
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• pp.126-138
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• 1995

To investigate the changes of physicochemical properties of leaf tobacco during storage, cured leaves (both flue-cured(KF109) and burley(Burley 21) : 4 grades of leaves) were stored under the natural warehouse condition(room temperature) and processed leaves(both Rue-cured(NC82) and burly(Burley 21) : 6 grades of leaves) were stored at storerooms in tobacco processing plants(flue-cured Cheongju plant ; 2nd, 4th and 5th floor, burley : Kwangju plant ; 2nd floor, Chonju plant : 3rd and 5th floor). Tobacco leaves were sampled and analyzed every 3 months. Total sugar content of flue-cured leaf decreased slightly and the redness degree of leaf increased after 15 months' storage under the natural warehouse conditions. The pH of cured leaves were lowered both flue-cured and burley, and the decreasing rate of pH was large in flue-cured(0.24) as compared with burley(0.14). There was no significant differences of physicochemical properties of processed leaf among storerooms during 15 months' storage. The decreasing rate of processed leaf pH was somewhat large in flue-cured(0.26) as compared with burly (0.20), and in thick leaves as compared with thin leaves. The redness degree of flue-cured leaf increased slightly, while the degree of lightness and yellowness lowered slightly during storage. The lightness degree of burley leaf lowered slightly, too. The ageing process of cured leaf was similar to that of processed leaf, it is considered that the passing days after curing will be more reasonable than the passing days after processing for the establishment of proper ageing period.

• 한국연초학회지
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• 제16권1호
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• pp.26-33
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• 1994

A tobacco ripeness grading meter was designed and constructed using the color sensor, its performance was evaluated. A degree of ripeness grading of a leaf is very closely related to the measured tobacco leaf color. Measuring the small amount of the reflectance precisely depends on the apparatus including color sensor, light source, detector sensitivity, and geometric characteristics of appratus. To analyze and minimize the variational effects, experiments to select the proper condition were performed. Because of the combined effect mentioned above, the system has some variation on its response. Basis on the results of the experiments, prototype was developed and interfaced to a computer system. The main components of prototype included a tungsten lamp as a light source, Amorphous full color sensor with three filters, regulated D.C. power supply, OP - AMP(741 TC) for amplification, AR - B3001 board for interfacing to a computer with analog to digital conversion, and a compatible IBM PC XT computer. The experimental results of the developed ripeness tobacco leaf measurement system are summarized as following: [1] The output readings of ripeness grade meter for tobacco leaf, which is based on harvesting time, showed the apparent difference in variety of different quality. It was considered suitable that three filters(red, green, blue) in Amorphous full color sensor could be used in four different ripeness degree measurement of tobacco leaf. [2] The output readings of ripeness grade meter for tobacco leaf, which is based on government procurement, showed apparent difference in variety of different quality. Tobacco leaf varieties to stalk position are divided into tips, leaf, cutters, and primings, It is considered suitable that only red filter in the sensor could be used to classify the grade of tobacco leaf within the same kind tobacco stalk. However, the ripeness grade meter was not adequate to classify all the tobacco grades in the four different tobacco leaves.

• 한국연초학회지
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• 제18권2호
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• pp.107-112
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• 1996

The occurrence and chemical properties of grey tobacco leaves (Type III) found in 1995 crop of flue-cured tobacco cultivated in paddy field were investigated to compare with those of normal leaves. Cured leaves of NC82 were separated by visual characters into 3 classes of slight, fair and severe symptoms of grey leaf by the percentage of grey parts to whole leaf area. Number of samples classified with discoloring portion was the order of greyish brown > reddish brown > greyish yellow, respectively. Grey leaves of this type were mostly found among the leaves harvested from upper stalk position, and it was estimated that growth rate of upper leaves also influenced on the occurrence of grey leaves. Grey leaves showed remarkably lower b* and L* values than those of normal leaves, while a* value was mostly higher in grey leaves. These tendencies in chromatic aberration showed more remarkable difference in the degree of grey symptoms. Chemical analyses of grey leaf samples indicated that they contained less total nitrogen and nicotine, and more total sugar and starch than those of normal leaves. In chemical traits, these tendencies were accordance with the degree of grey symptoms, and within the same leaf, grey parts were decreased in total nitrogen and ether extract content compared with those of normal parts, but there was no difference in nicotine and Cl contents. Key words : grey leaf tobacco (type III), grey symptom, color, chemical properties.

• 한국연초학회지
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• 제22권1호
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• pp.25-30
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• 2000

Bacterial wilt(Ralstonia solanacearum) is one of the major diseases of flue-cured tobacco (Nicotiana tabacum L.) in the world. This study was conducted to investigate degree of dominance, selection, and correlation between leaf shape and degree of bacterial wilt resistance in flue-cured tobacco. The degree of disease caused by bacterial wilt was evaluated in parents, F$_1$, F$_2$ and F$_3$ populations of two crosses, BY 4 x NC 95 and BY 4 x Coker 86, in the infected field. The leaf shape index was also measured in parents and F$_2$ population of BY 4 x NC 95. The incidence of bacterial wilt was observed in the middle of June and peaked in late July, when the highest value of pathogen density reached 1.0 x 10$^{6}$ colony forming unit per gram. It was concluded that the inheritance mode of risestance to bacterial wilt in the above two crosses of susceptible and resistant varieties was recessive and polygenic. The resistance to bacterial wilt was significantly correlated with leaf shape in F2 generation of BY 4 x NC 95. But certain plants having narrower leaves were also resistant to bacterial wilt. It is considered that the bacterial wilt resistant lines having narrower leaves could be selected. The selection for bacterial wilt resistance in the F$_2$ population might be effective.

• 한국연초학회지
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• 제20권2호
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• pp.139-143
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• 1998

The occurrence and physico-chemical properties of flat leaves found in 1997 curing trial related to the prevention of occurrence of flat leaves at bulk curing of flue-cured tobacco were investigated and compared with those of normal leaves. Cured leaves of NC82 were separated into 4 classes of none, slight, fair and severe symptoms of flat leaf by the percentage of flattened parts to whole leaf area. The flat leaves were mostly found among the leaves of lower stalk position, and it was estimated that growth rate of lower leaves also influenced on the occurrence of flat leaves. In chromatic aberration of cured leaf, flat leaves showed remarkably lower b and L values than in those of normals but there was no difference in a value. On the other hand, in chemical analyses of flat leaf samples, nicotine, total sugar, ether extract and total nitrogen contents were decreased with the degree of flat symptoms. In physical properties, filling capacity of cured leaves was decreased with the degree of flat symptoms comparing with those of normal leaves, while shatter index was showed a reverse tendency, and then within the same leaf, flat parts were decreased in total sugar, ether extract and filling capacity compared with those of normal ones, but remarkably increased in shatter index, and there was no difference in nicotine and total nitrogen contents.

• 한국연초학회지
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• 제18권1호
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• pp.66-75
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• 1996

This study was carried out to investigate the effects of high temperature and humidity on the ageing of processed leaf tobacco. Four(1993) or six(1994) grades (Both flued-cured and burley) of processed leaf packed in carton box were stored under the natural and artificial conditions. When the Processed leaf was stored in the controlled mom at 40℃ with 75% R.H.(1993) for 40 days, the leaf pH was decreased. The decreasing rate of leaf pH was similar to that of leaf stored under the natural warehouse condition for 15 months. The degree of lightness and yellowness of leaf also decreased, while the decaying or darkening of the leaf was observed. When the processed leaf was stored in the controlled room at 35℃ with 65% R.H.(1994) for 90 days, the pH of flue-cured was decreased 0.22, which was similar to that of the leaf stored under the natural warehouse condition for 15 months, and the lightness, and redness of the leaf were higher than those of the control. As compared with the leaf stored under the natural condition for 2 years, the smoking quality of leaf stored under this condition was similar or somewhat better. The pH of burley tobacco changed little compared to that of flue-cured during storage in this study.

• 한국연초학회지
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• 제27권2호
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• pp.226-234
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• 2005

The uncertainty of measurement in nitrate from burley leaf tobacco by continuous-flow analysis method was evaluated following internationally accepted guidelines. The sources of uncertainty associated with the analysis of nitrate were weight of standard and sample, purity of standard, dilution of standard solution, calibration curve, water content, etc. The calculation of uncertainty based on the GUM(Guide to the Expression of Uncertainty in Measurement) and EURACHEM/CITAC Guide. An expanded uncertainty was obtained by multiplying the combined standard uncertainty with a coverage factor (k) calculated from the effective degree of freedom. The concentration of nitrate from burley leaf tobacco was $2.09\%$ and the expanded uncertainty by multiplying by the coverage factor(k, 2.20) was $0.13\%\;at\;a\;95\%$ confidence level.

• 한국연초학회지
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• 제23권2호
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• pp.149-155
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• 2001

This study was carried out to evaluate the correlations among degree of carbonization, chemical components and chromatic characteristics, and to establish the criterion of carbonizaion in leaf tobacco during aging storage. The flue-cured (B2O) and burley (B2T) leaf tobaccos were redried with conventional practices for each other type, and moisture contents of leaf tobaccos were adjusted to 13, 15, 17 and 19% after redrying. The leaf tobaccos were packed in carton box and stored for 8 months in the controlled-environment rooms($35^{\circ}C$, 65% R.H. and $40^{\circ}C$, 80% R.H.). Degree of carbonization, chemical components, and chromatic characteristics(L ; black-white, a ; red to green, b ; yellow to blue) were invetigated every month. Carbonization Index (C.I.) was established as (equation omitted) in accordance with degree of carbonization in two types. The C.I. value of flue-cured leaf(56.5) for slight carbonization was higher than that of burley leaf(48.0). C.I. and rate of usable leaf, L and b were positively correlated in two types. C.I. was significantly shown positive correlation with pH in two types. The correlations between the predicted and the observed values of slight carbonizating times(month after storage) using C.I. and pH were positive significant in two types. The results suggest that cabonization index may be useful to forecast and prevent the carbonization, and pH may be useful to estimate cabonization indirectly for flue-cured and burley leaves during aging storage.

• 한국연초학회지
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• 제23권1호
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• pp.19-30
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• 2001

This study was carried out to investigate the influences of environmental condition in storage room, moisture content and packing weight of leaf on the carbonization of processed flue-cured leaf tobacco, and find out a regression model able to pre-estimate the degree of carbonization. The influence of temperature and humidity in storage room on the carbonization was high,, while that of packing weight was relatively low, However, high moisture content and packing weight of leaf accelerated the carbonization under the high temperature and humidity condition. Thus the leaf tobacco under the condition of 4$0^{\circ}C$, 7$0^{\circ}C$ R.H. in storage room, 16% moisture content and 286 kg/box of packing weight could be carbonized within one month. The pH, total sugar content, lightness(L) and yellowness(b) of leaf were closely related to carbonization of leaf during storage. There were significant regression and yellowness of leaf. It is considered that the certification and/or pre-estimation of carbonization during storage may be possible by applying the regression equation.

• 한국연초학회지
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• 제9권2호
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• pp.25-35
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• 1987

Effects of application time And rate of chlorine on the chlorine absorption and distribution at different stalk positions of the plants were Investigated in burley tobacco grown under the pot condition. Treatment consisted of 6 application times (from 2 weeks before transplanting to 8 weeks after transplanting with 2 weeks' interval) and 2 application rates (2g and 4g-KCl/pot) by 6X2 factorial experimental design. The tobacco plants took up the chlorine rapidly just after chlorine application, and the chlorine content of leaf was increased proportionally to the rate of chlorine application. The chlorine was accumulated when much in lower leaves when chlorine was applied early, while the chlorine accumulated much in upper leaves when chlorine was applied late. The chlorine content of leaf was negatively correlated to the degree of brightness, red color and yellow color of cured leaf. Significant Increases In cured leaf ratio, leaf thickness, leaf weight and weight Per unit leaf area of harvested leaf were obtained with increased chlorine application. When the chlorine was applied early, the leaf weight showed Increasing tendency.