• 제목/요약/키워드: defined medium

검색결과 478건 처리시간 0.03초

자동화 시스템내 셀 콘트롤러의 통신소프트웨어 개발 및 실험

  • 정병수;도성희;박경진;강무진
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 1992년도 추계학술대회 논문집
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    • pp.243-248
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    • 1992
  • Computer Integrated Manufacturing(CIM) is a growing technology as a solution for the total automation of a manufacturing system. However, the implementation in the shop floor is extremely difficult due to many reasons. Flexible Manufacturing System (FMS) is usually considered as a solution for the shop floor automation. One of the difficulties in FMS is the communications problem. Since various machinaries with different communications protocols are included, applying a unified scheme is almost impossible. Therefore, a systematic approach is a key point to solve the communication problem. A cell is defined as an automation unit where closely related for a job reside together. A cell is a messenger between upper level computers and lower level machine equipment. In this research, the functions of the cell are defined to have more capabilities than conventional cell since a cell can be often a total manufacturing system in a small to medium sized factory. The cell conducts communications with different machines through the communications schemes established here. A set of software system has been developed according to the defined communications. The software has been tested for a simulation and real experiments for proof.

Xylose 발효효모의 Xylanase 생성 (Nutritional Conditions of Xylanase Production from Xylose Fermenting Yeast)

  • 배명애;김남순;방병호;서정훈
    • 한국미생물·생명공학회지
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    • 제17권2호
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    • pp.85-87
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    • 1989
  • 토양으로부터 분리한 Xylose 발효효모 X-6-41 (Candida sp.)의 xylanase 효소생성 조건을 조사해 본 결과, 본 실험에 사용한 X-6-41 균주는 배지조성중 yeast extract 혹은 polypeptone이 첨가되면 xylanase 효소생성이 없었다. 즉 특정 아미노산인 isoleucine에 의해서는 그 생성이 억제되었고, tryptophan 25$\mu$g/$m\ell$ 농도의 존재하에서는 xylanase 생성이 증가했다.

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중형 엔진 터보차져의 원심압축기에 관한 공력학적 3차원 형상 및 구동용 연소기 설계 (Aerodynamic Three Dimensional Geometry and Combustor Design for the Compressor of the Medium Speed Diesel Engine Turbocharger)

  • 김홍원;류승협;갈상학;하지수;김승국
    • 유체기계공업학회:학술대회논문집
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    • 유체기계공업학회 2005년도 연구개발 발표회 논문집
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    • pp.517-524
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    • 2005
  • An aerodynamic design for centrifugal compressor which was applied to medium speed diesel engine has done. First of all, exact compressor specifications must be defined by accurate engine system matching. This matching program has been developed. Using the mean1ine prediction method, geometric design and performance curve for compressor was done and verified by comparing three dimensional viscous CFD results. The deviation at the design point was about 2.3%. Combustor has been designed and manufactured for the performance test of medium speed diesel engine turbocharger. Fuel nozzle of combustor was designed and performed by PIV and PDPA test equipment. Through these results, spray characteristics were studied and flow coefficient equation was deduced.

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The Benefit of Ambiguity Resolution Using Triple Frequency

  • Tominaga, Reiji;Gomi, Yasuto;Zhang, Yun;Kubo, Nobuaki;Yasuda, Akio
    • 한국항해항만학회:학술대회논문집
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    • 한국항해항만학회 2006년도 International Symposium on GPS/GNSS Vol.2
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    • pp.23-26
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    • 2006
  • Modernized GPS will have three frequencies modulated with three signals, which will be accessible to all users in the near future. This new frequency provides an opportunity to resolve the double differenced (DD) integer ambiguity very fast and with almost no baseline constraints. In order to study the performance of triple frequency system for Ambiguity Resolution (AR) over the medium baseline under different ionospheric levels, the Klobuchar Model was implemented and used in our triple simulation to generate the ionospheric delay. Furthermore, the White-Gaussian noise applying to distance-dependent parameters was added to the DD ionospheric delay. For medium baseline (defined as here 20 to 40kms), success rates of AR has been pretty improved. In this paper, the medium baseline AR strategies that take advantage of carrier phase measurement on the third frequency will be discussed.

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Candida albicans의 시험관 희석법에 의한 항균력 검사시 배지가 항균력에 미치는 영향 (Effect of Culture Medium on Results of Maerobroth Dilution Antifungal Susceptibility Testing of Candida albicans)

  • 고춘명;김수기
    • 대한미생물학회지
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    • 제22권3호
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    • pp.301-307
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    • 1987
  • A total of 42 strains of Candida albicans were examined for susceptibility to three antifungal agents, amphotericin B(AMB), 5-fluorocytosine(5-FC), and ketoconazole(KTZ), using defined medium, synthetic amino acid medium-fungal(SAAM-F), supplemented yeast nitrogen base(SYNB) and undefined medium Sabouraud's dextrose broth(SDB) and Kimmig broth media. A tube dilution method was used with minimum inhibitory concentrations(MICs) determined after incubation for 24 hour and 48 hours. All testes were performed in duplicate. In general, MICs were more reproducible after 48 hour of incubation. Forthermore, MICs determined after incubation for 48 hours were significantly higher than those determined after 24 hours. The actural MICs obtained with the different antifungal agents were clearly influenced by the test medium used. The rank order of AMB MICs according to the test medium was as follows: SAAM-F>SYNB>SDB>Kimmig broth. With 5-FC, the following pattern was observed: SYNB>SAAM-F>SDB>Kimmig borth. For ketoconazole, the MICs according to the test medium was SAAM-F>SDB>SYNB> Kimmig broth. In amphotericin B, the MICs mean value with the test medium was as follows: SDB, 0.24 mcg/ml; Kimmig broth, 0.29 mcg/ml; SYNB, 0.21 mcg/ml and SAAM-F, 0.15mcg/ml. The actural value of 5-FC was; SDB, 37.20 mcg/ml; Kimmig broth, 67.41mcg/ml; SYNB, 21.29 mcg/ml and SAAM-F, 24.61 mcg/ml and in ketoconazole, the MICs value was; SDB, 1.83 mcg/ml; Kimmig broth, 4.08 mcg/ml; SYNB, 1.95 mcg/ml and SAAM-F, 1.41 mcg/ml. The results of this investigation suggested that broth dilution susceptibility testing of yeast and yeast-like fungi are best performed with an incubation period of 48 hours. Furthermore, medium composition can significantly influence the results of such testing.

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호르몬, 아미노산 및 단백질이 첨가되지 않는 단순배양배지내 소 난포란의 탄수화물 요구량 (Carbohydrate Requirements of Follicular Bovine Oocytes Cultured in a Chemically Defined, Hormone-, Amino Acid- and Protein-Free Medium)

  • 임정묵
    • 한국발생생물학회지:발생과생식
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    • 제1권1호
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    • pp.1-8
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    • 1997
  • 본 연구는 단순배양체계내 소 난포란의 성숙에 요구된는 탄수화물 (glucose, lactate와 pyruvate)을 검토하기 위해 수행하였다. GV(germinal vesicle) 단계의 난구세포로 둘러싸인 소 난자를 단백질, 아미노산 및 호르몬이 첨가되지 않은 modified Tyrodes (mT)에서 24시간, 5% $CO_{2}$ 배양기를 이용하여 성숙배양하였다. Glucose 무첨가군 (0-61%)에 비해 5.6mM의 glucose 첨가군 (71-74%)이 유의적으로 높은 (P<0.05) M-II단계로의 난자 발육을 나타내었다. Glucose를 함유한 배지내에서는 lactate(10mM0와 pyruvate(0.5mM)의 첨가에 따르는 M-II 단계로의 발육에 있어 차이를 보이지 않았다. 그러나 glucose 무첨가 배지에서는 pyruvate와 lactate를 첨가하는 것이 첨가하지 않은 것에 비해 condensed GV(76%vs. 0-2%), M-II, (43-61%vs. 0%)단계에 이른 난자수가 유의적으로 높았다. Glucose 함유 mT배지에 lactate와 pyruvate를 첨가하여 난자를 배양하였을 때, 동결융해 정자와 24시간 정치한 후 난자중 87-93%가 정자침투되었고 39-44%가 전핵단계로 발육하였다. 침투난자의 26-30%는 다정자수정이었다. 결론적으로, GV 단계의 소 난포란은 에너지원으로 lactate, glucose와 Pyruvate를 이용하지만, 감수분열 성숙을 유지하는데 glucose가 가장 효과적이다. 이 결과들로 보아 단순배양배지는 소 난포란의 체외성숙에 영향을 미치는 다양한 물질들을 연구하는데 잠재적으로 유용하다.

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Stochastic MAC-layer Interference Model for Opportunistic Spectrum Access: A Weighted Graphical Game Approach

  • Zhao, Qian;Shen, Liang;Ding, Cheng
    • Journal of Communications and Networks
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    • 제18권3호
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    • pp.411-419
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    • 2016
  • This article investigates the problem of distributed channel selection in opportunistic spectrum access networks from a perspective of interference minimization. The traditional physical (PHY)-layer interference model is for information theoretic analysis. When practical multiple access mechanisms are considered, the recently developed binary medium access control (MAC)-layer interference model in the previous work is more useful, in which the experienced interference of a user is defined as the number of competing users. However, the binary model is not accurate in mathematics analysis with poor achievable performance. Therefore, we propose a real-valued one called stochastic MAC-layer interference model, where the utility of a player is defined as a function of the aggregate weight of the stochastic interference of competing neighbors. Then, the distributed channel selection problem in the stochastic MAC-layer interference model is formulated as a weighted stochastic MAC-layer interference minimization game and we proved that the game is an exact potential game which exists one pure strategy Nash equilibrium point at least. By using the proposed stochastic learning-automata based uncoupled algorithm with heterogeneous learning parameter (SLA-H), we can achieve suboptimal convergence averagely and this result can be verified in the simulation. Moreover, the simulated results also prove that the proposed stochastic model can achieve higher throughput performance and faster convergence behavior than the binary one.

Bacillus sp.에 의한 균체외 단백질의 생산 (Production of Extracellular Protein from Bacillus sp.)

  • 이재숙;김찬조;이종수
    • Applied Biological Chemistry
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    • 제31권2호
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    • pp.187-192
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    • 1988
  • 미생물에 의한 단백질 생산과 그의 분비기작에 관한 기초 자료를 얻고자 토양으로 부터 균체외 단백질 생산균주를 분리하고 동정한 결과 Bacillus sp.로 추정 되었다. 선정균주를 3.0%의 glucose와 1.3%의 urea를 첨가한 chemically defined medium에 접종하여 $30^{\circ}C$에서 48시간 배양하였을때 1.2mg/ml의 단백질을 생산 하였고 $CaCO_3$의 첨가로 단백질 생산이 촉진 되었으며 $250{\mu}g/ml$의 cephalexin를 첨가하여 96시간 배양 하였을때 2.0mg/ml의 단백질을 생산 하였다.

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기술연관분석을 활용한 기술융합구조 분석: 중소기업 융·복합기술개발사업 사례 (Analysis of Technology Convergence Structure Using technology Input-output Analysis: Case of Convergence R&D Development Project for Small and Medium Businesses)

  • 이광민;김다운;홍재범
    • 기술혁신연구
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    • 제22권3호
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    • pp.1-35
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    • 2014
  • 본 연구는 기술연관분석으로 단기 상용화를 위한 기술개발과제에 투입된 기술간 융합현황을 분석하였다. 기술연관분석은 산업구조분석의 체계를 기술기획 분야에 적용한 것이다. 본 사례는 기술연관분석을 활용하여 기술과 제품 간 연관도를 중심으로 분석하였으며 분석대상은 중소기업기술정보진흥원의 ' '12년 중소기업 융 복합기술개발사업'에 신청한 401개 과제이다. 분석과정은 다음과 같다. 401개 개별과제에 대해서 투입된 기술은 기술로, 개발과제에서 산출을 제품으로 정의하고 이를 투입과 산출의 행렬로 정리하였다. 투입으로 정의한 기술은 국가과학기술표준분류 상의 소분류를 분류하고, 산출로 정의한 제품은 한국표준산업분류 상의 세세분류로 정의하였다. 즉, 기술을 투입으로, 제품을 산출로 정의하여 기술의 파급도를 분석하였으며 이에 기초하여 기술연관도를 작성하였다 분석결과, IT융합에서는 임베디드 S/W의 기술파급도가 가장 높았으며, ET융합에서는 금형의 기술파급도가 가장 높았고 BT융합은 기능성 화장품 개발기술의 기술파급도가 가장 높았다. 전체적으로 IT융합에서 기술파급도가 높은 요소기술이 많았으며, ET융합에서 기술파급도가 높은 요소기술이 작았다. 따라서 기술파급도가 높은 요소기술에 대한 투자가 융합을 활성화하는 데 중요하며 전체적으로 IT분야가 요소기술의 투자 효과가 높을 것으로 판단한다.

진균류(眞菌類)의 상호작용(相互作用)에 의한 Aflatoxin 생성능(生成能)에 관(關)한 연구(硏究) (Effect of Various Fungi on the Aflatoxin Productivity in tile Culture of Asp. Flavus)

  • 김운주;정용;권숙표
    • Journal of Preventive Medicine and Public Health
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    • 제9권1호
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    • pp.77-86
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    • 1976
  • This study was attempted to know that the interactions of various fungi, and methionine and $MgSO_4$ introduced as the substrate of culture media for fungi were affected to produce aflatoxins by Asp. flavus. 5 different fungi were isolated from the fermented soybean mash and were cultured in Chemically Defined medium (C. D. media) and soybean mash at $25^{\circ}C$ for 10 days. (1) It was confirmed that Asp. flavus produced aflatoxins in the C. D. medium and soybean mast, but that Asp. niger, Asp. oryzae, Asp. awamori and Asp. terreus did not produced them respectively. (2) Asp. flavus cultured with Asp. niger did not produce aflatoxins in C. D. medium, but produced in soybean mash, in other hand, Asp. flavus with other fungi except Asp. niger produced aflatoxins in C. D. medium and soybean mash. (3) The growth of fungi were more prosperous in the seperate culture than in the mixed culture. (4) In the C. D. medium added 20% of cultured medium of Asp. niger, Asp. flavus did not produce aflatoxins but other cultured medium did not prohibit the production of aflatoxins by Asp. flavus. (5) On the contrary, $MgSO_4$ increasing the productivity of aflatoxins by Asp. flavus in the C. D. medium, methionine known as one of precursor of aflatoxins did not affected the increasing productivity with significance.

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