• Microbiology and Biotechnology Letters
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• v.33 no.4
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• pp.281-287
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• 2005

The endoinulinase gene (inu, 2.733 kb, EC 3.2.1.7) from Paenibacillus polymyxa was subcloned into an Escherichia coli-yeast shuttle vector with GALl promoter for the expression in Saccharomyces cerevisiae. The constructed plasmid, pYGENIU27 (8.6 kb) was introduced into S. cerevisiae SEY2102 cell and then the yeast transformant was selected on the synthetic defined media lacking uracil and on the inulin-containing media. The recombinant endoinulinase was predominantly localized in the periplasmic space of the yeast cell. The total activity of the endoinulinase reached 1.81 unit/ml by cultivation of yeast transformant on YPDG medium. The optimized conditions determined for the inulooligosaccharides (IOSs) production from inulin were as follows; pH, 8.0; reaction temperature, $45^{\circ}C$; inulin source, Jerusalem artichoke. Enzyme activity was stably maintained up to the pH of 10.0. Under the optimized condition and with endoinulinase of 36 unit/g-inulin, IOSs started to be produced after 10 min of enzymatic reaction. By the reaction with inulin, IOSs consisting of inulobiose (F2), inulotriose (F3), and inulotetraose (F4) were produced and F3 was the major product. Consequently, these data would be used as a fundamental parameters for the production of functional sweetener IOSs from inulin by recombinant yeast endoinulinase.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.1
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• pp.705-713
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• 2018

An automotive ball joint connects the suspension system to the steering system and helps to enable rotational and linear motion between the two elements for steering. This study examines a ball joint used in medium and large-sized pickup trucks. Ball joints consist of a stud, socket, bearing, and plug. The main structural performance metrics of ball joints are the pull-out strength and push-out strength. These structural parameters must meet certain criteria to avoid serious accidents. Test and simulation methods are used to investigate the design requirements, but tests are time-consuming and costly. In this study, we modeled ball joints in SolidWorks and performed a finite element analysis in Abaqus to predict structural performance. The analysis was used to obtain the structural performance required for the static analysis of a 2D axisymmetric model. The uncertainties in the manufacturing of the ball joint were assumed to be the manufacturing tolerances, and the dimensional design variables were identified through case studies. The manufacturing tolerances at each level were defined, and the results were compared with experimental results.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.5
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• pp.1460-1466
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• 2008

The purpose of this study is to verify how the accounting information of a bankrupt firm which is defined as a dishonor, an impaired total capital, a poor financial performance of a business, a rejection of auditor's opinion and an incongruity of auditor's opinion differs from that of a healthy firm on the basis of the index of financial affairs if the accounting information released by KOSDAQ is valuable. The sampling firms consists of 45 KOSDAQ firms that went bankrupt from 2000 to 2007 and 45 healthy firms which are selected in accordance with the sizes of assets. It has also selected the 30 sampling firms for the confirmation of the model in the same way. According to the result of the in-depth analysis, the variables related to security among the 17 indexes of financial affairs that have been used in this study for 5 years show a noticeable difference between a bankrupt firm and a healthy one. The accuracy of failed firms using this model for confirmation demonstrates 76.7% in 5 years before the bankruptcy, 76.7% in 4 years before that, 65.0% in 3 years before it, 76.7% in 2 years, 88.3% in 1 year. This data shows that the process from a healthy firm to a bankrupt one has progressed gradually and confirms the value of the index of financial affairs, exhibiting the accuracy with 83.8% of a presuming sample and 76.7% of a confirming sample for 5 years.

• Microbiology and Biotechnology Letters
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• v.34 no.2
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• pp.101-108
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• 2006

YNB54 strain which shows inhibitory activities specific to the plant pathogenic Phytophthora sp. on potato dextrose agar medium was screened among lots of strains isolated from Korean soils. To identify taxonomy of the Phytophthora specific antagonistic bacteria YNB54, 165 rDNA sequence, MIDI fatty acid composition, DNA-DNA hybridization, GC content, and commercial multitest systems such as API 20E and Biolog GN were performed. Results of commercial kits including lots of biochemical and physiological reactions showed that this strain was closely related to taxa including Enterobacter cloacae and Enterobacter cancerogenus species than other genera(Citerobacter Klebsiella, Leclercia). Also, analysis of its MIDI, G+C contents, and DNA-DNA hybridization suggests that this strain was more similiar to the Genus Enterobacter than other genera (Citerobacter Klebsiella, Leclercia). This strain was potentially identified as Enterobacter sp. by these results. But our 16S ribosomal DNA sequences (rDNA) analysis confirmed that it was more closely related to the cluster of Citerobacter freundii ATCC 29935 than any other Enterobacter species. In the absence of defined phylogenetic critia for delineating genera, the results observed with Citrobacter and Enterobacter species suggest that further studies are needed to clarify their relationships. This investigation demonstrates that YNB54 strain is genetically diverse and potentially more taxonomically complex than hitherto realized. Further study is necessary to confirm their taxonomic positions.

• The Journal of the Petrological Society of Korea
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• v.11 no.2
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• pp.65-73
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• 2002

The wavy extinction of quartz grains can be used as a good indicator to show the degree of rock deformation. To determine degree of the rock deformation, intensity of wavy extinction (IWE) of quartz grains was measured by using polarizing microscope, digital camera, and NIH image (or Scion Image). In this experiment, the measurement was performed along the line perpendicular to the subgrain boundary of wavy extinction. IWE was measured when a quartz grain shows maximum extinction in polarizing microscope. NIH image was represented in terms of 256 gray-scale brightness, which was associated with intensity of the wavy extinction. IWE was determined by the degree of brightness versus the measuring length of a quartz grain. IWE was analysed in the distribution chart with the collection unit of 5. Regarding the characteristics of data distribution, the median between the mode and the median of the histogram is defined as a representative value for the IWE, and this value is subdivided into the five levels of rock deformation zones (lowest- or non-, low-, medium-, high-, and highest deformation) with their respective indices (D1, D2, D3, D4, and D5). The correlation between the Eonyang Granite and the Yangsan Fault was investigated by using the indices. The results indicate that IWE values decreased away from the fault.

• Journal of the Korea Society for Simulation
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• v.23 no.4
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• pp.51-64
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• 2014

Small and medium Enterprises (SMEs), which have a manufacturing method of small quantity batch production produce goods using a general-purpose equipment and attached auxiliary tools. Many previous studies have focused on finding the effective resource allocations for improving the firms' productivity. It is very important for SMEs to keep costs low in assigning jobs to each resource, because they should meet the future uncertain demand of consumers under the limited budget. Using the concept of salvage cost, this paper proposes how to effectively allocate the tasks to main resources in the production process. The salvage cost is defined that purchasing cost minus decrease in value by workload, the method considering this is expected to reduce total purchasing costs during business period. To validate the effect of the proposed method, we proceed the real case study targeting on S company, PCB manufacturer to compare purchase amounts and its costs between the allocation proposed based salvage cost and current allocation method of current S company. As a results, In short-term (3 year) business period, salvage allocation have remarkable superior outcome to existing method, but gradually have cancelled out the effects in long-term (8 year) plans. Unlike the cycle allocation method, there exists the idle-equipments in allocation based salvage value. we additionally analyze the profits with respect to rental strategy of them during business period.

• Nuclear Engineering and Technology
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• v.28 no.2
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• pp.175-184
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• 1996

A nuclide transfer by utilizing mass transfer coefficient and barrier response function defined for each barrier is proposed, by which the final nuclide transfer rate into the sea water can be evaluated. When simple and immediate quantification of the nuclide release is necessary in the conservative aspect, using this kind of approach may be advantageous since each layered barrier can be treated separately from other media in series in the repository system, making it possible to apply separate solutions in succession to other various media. Although one disadvantage is that while flux continuity can be maintained at the interface by using the exit nuclide flux from the first medium as the source flux for the next one, there may be no guarantee for concentration continuity, this problem could be eliminated assuming that there is no boundary resistance to mass transfer across the interface. Mass transfer coefficient can be determined by the assumption that the nuclide concentration gradient at the interface between adjacent barriers remains constant and barrier response function is obtained from an analytical expression for nuclide flow rate out of each barrier in response to a unit impulse into the barrier multiplied by mass transfer coefficient. Total time-dependent nuclide transfer rate from the barrier can then be obtained by convoluting the response function for the barrier with a previously calculated set of time-varying input of nuclide flow rate for the previous barrier.

• Safety and Health at Work
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• v.10 no.4
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• pp.482-503
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• 2019

Introduction: A new third version of the Copenhagen Psychosocial Questionnaire (COPSOQ III) has been developed in response to trends in working life, theoretical concepts, and international experience. A key component of the COPSOQ III is a defined set of mandatory core items to be included in national short, middle, and long versions of the questionnaire. The aim of the present article is to present and test the reliability of the new international middle version of the COPSOQ III. Methods: The questionnaire was tested among 23,361 employees during 2016-2017 in Canada, Spain, France, Germany, Sweden, and Turkey. A total of 26 dimensions (measured through scales or single items) of the middle version and two from the long version were tested. Psychometric properties of the dimensions were assessed regarding reliability (Cronbach α), ceiling and floor effects (fractions with extreme answers), and distinctiveness (correlations with other dimensions). Results: Most international middle dimensions had satisfactory reliability in most countries, though some ceiling and floor effects were present. Dimensions with missing values were rare. Most dimensions had low to medium intercorrelations. Conclusions: The COPSOQ III offers reliable and distinct measures of a wide range of psychosocial dimensions of modern working life in different countries; although a few measures could be improved. Future testing should focus on validation of the COPSOQ items and dimensions using both qualitative and quantitative approaches. Such investigations would enhance the basis for recommendations using the COPSOQ III.

• The Journal of the Petrological Society of Korea
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• v.19 no.4
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• pp.283-292
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• 2010

The Suljeongri east three-story stone pagoda in Changnyeong (National Treasure No. 34) has been damaged mainly by lots of cracks. The stones used for this pagoda are medium-granied equigranular pinkish biotite granite. Measured magnetic susceptibility values are of from 2 to 9 (${\times}10^{-3}$ SI unit). From the ${\gamma}$-ray spectrometer mesurement K, eU, and eTh contents of the stones are 3 to 7%, 8 to 19 ppm, and 11 to 35 ppm, respectively. Comparing the petrographical and chemical characteristics between the stones of the pagoda and the country rocks near Suljeongri, it is suggested that the most similar rock could be equigranular biotite granite in the western slope of the Mt. Hwawangsan. Vertical, horizontal and diagonal cracks are intensely developed at the lower part of the stone pagoda. Biotite granite has intrinsic microcracks defined as rift and grain rock cleavages. Both rock cleavages are assumed to have led to the crack growth and consequent mechanical damage of the pagoda. It seems that vertical cracks have been grown parallel to the principal compressional stress, and that horizontal cracks to the reacting tensional stress. Diagonal cracks seems likely to have been resulted from conjugate rift and grain rock cleavages.

• Journal of Food Hygiene and Safety
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• v.26 no.2
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• pp.91-99
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• 2011

Risk ranking must be determined for various hazards/food combinations to conduct microbial risk management effectively. Risk Ranger is a simple, easy-to-use calculation tool developed in Microsoft Excel and designed to rank the risk (low, medium, and high) for semi-quantitative microbial risk assessment. The user is required to answer 11 questions in Risk Ranger related to 1) severity of the hazard, 2) likelihood of a disease-causing dose of the hazard being present in the meal, and 3) the probability of exposure to the hazard in a defined time. This study determined the risk ranking for twenty three combinations of foodborne pathogens/potentially hazardous foods (PHFs) using a Risk Ranger. In this study, pathogenic E. coli in fresh cut produce salad was scored as 79, which was the highest rank among the 23 combinations of the foodborne pathogens and PHFs. On the other hand, zero risk was obtained with V parahaemolyticus in sushi, Salmonella in meat products and E. coli O157:H7 in hamburger patties. Although Risk Ranger is very simple method to rate the risk of foodborne pathogens and PHFs combination, the accuracy of result was mainly affected by the availability and accuracy of data in the literature. According to the result of literature review, the data are needed for contamination rate of raw materials, consumption amount/frequency of PHFs, and the effect of processing on pathogen. Risk ranking must be continuously revalidated with new data.